Sequence of pAdTrack

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

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Sequence of pAdTrack

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Sequence of pAdTrack : 8175 BP; 1923 A; 2051 C; 2222 G; 1882 T; 97 other;

.......... .......... .......... .......... .......... ..........

1 nnnttaatta annntccctt ccagctctct gccccttttg gattgaagcc aatatgataa 60

1 nnnaattaat tnnnagggaa ggtcgagaga cggggaaaac ctaacttcgg ttatactatt 60

.......... .......... .......... .......... RITR ..........

.......... .......... .......... .......... .......... ..........

61 tgagggggtg gagtttgtga cgtggcgcgg ggcgtgggaa cggggcgggt gacgtagtag 120

61 actcccccac ctcaaacact gcaccgcgcc ccgcaccctt gccccgccca ctgcatcatc 120

.......... .......... RITR LITR .......... ..........

.......... .......... .......... .......... .......... ..........

121 tgtggcggaa gtgtgatgtt gcaagtgtgg cggaacacat gtaagcgacg gatgtggcaa 180

121 acaccgcctt cacactacaa cgttcacacc gccttgtgta cattcgctgc ctacaccgtt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aagtgacgtt tttggtgtgc gccggtgtac acaggaagtg acaattttcg cgcggtttta 240

181 ttcactgcaa aaaccacacg cggccacatg tgtccttcac tgttaaaagc gcgccaaaat 240

.......... .......... .......... .......... .......... Encap

.......... .......... .......... .......... .......... ..........

241 ggcggatgtt gtagtaaatt tgggcgtaac cgagtaagat ttggccattt tcgcgggaaa 300

241 ccgcctacaa catcatttaa acccgcattg gctcattcta aaccggtaaa agcgcccttt 300

.......... .......... Encap .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 actgaataag aggaagtgaa atctgaataa ttttgtgtta ctcatagcgc gtaannncgc 360

301 tgacttattc tccttcactt tagacttatt aaaacacaat gagtatcgcg cattnnngcg 360

.......... .......... Encap .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gttaagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt 420

361 caattctatg taactactca aacctgtttg gtgttgatct tacgtcactt tttttacgaa 420

.......... ........ EBV_rev_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca 480

421 ataaacactt taaacactac gataacgaaa taaacattgg taatattcga cgttatttgt 480

.......... .......... .......... .......... .......... ..........

HpaI(484) .......... .......... .......... .......... ..........

481 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt 540

481 tcaattgttg ttgttaacgt aagtaaaata caaagtccaa gtccccctcc acaccctcca 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag 600

541 aaaaatttcg ttcattttgg agatgtttac accataccga ctaatactag tcaatagatc 600

.......... .......... .......... .......... ORF2 ..........

.......... .......... .......... .......... .......... ..........

601 atccggtgga tctgagtccg gacttgtaca gctcgtccat gccgagagtg atcccggcgg 660

601 taggccacct agactcaggc ctgaacatgt cgagcaggta cggctctcac tagggccgcc 660

.......... .......... ORF2 ORF2 .......... EGFP ..........

.......... .......... .......... .......... .......... ..........

661 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg 720

661 gccagtgctt gaggtcgtcc tggtacacta gcgcgaagag caaccccaga aacgagtccc 720

...... EGFP_C_primer ORF2 ORF2 EGFP ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg 780

721 gcctgaccca cgagtccatc accaacagcc cgtcgtcgtg ccccggcagc ggctaccccc 780

.......... ........ ORF2 ORF2 EGFP ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct 840

781 acaagacgac catcaccagc cgctcgacgt gcgacggcag gagctacaac accgcctaga 840

.......... ........ ORF2 ORF2 EGFP ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt 900

841 acttcaagtg gaactacggc aagaagacga acagccggta ctatatctgc aacaccgaca 900

.......... ........ ORF2 ORF2 EGFP ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct 960

901 acatcaacat gaggtcgaac acggggtcct acaacggcag gaggaacttc agctacggga 960

.......... ........ ORF2 ORF2 EGFP ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt 1020

961 agtcgagcta cgccaagtgg tcccacagcg ggagcttgaa gtggagccgc gcccagaaca 1020

.......... ........ ORF2 ORF2 EGFP ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg 1080

1021 tcaacggcag caggaacttc ttctaccacg cgaggacctg catcggaagc ccgtaccgcc 1080

.......... ........ ORF2 ORF2 EGFP ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt 1140

1081 tgaacttctt cagcacgacg aagtacacca gccccatcgc cgacttcgtg acgtgcggca 1140

.......... ........ ORF2 ORF2 EGFP ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga 1200

1141 tccagtccca ccagtgctcc cacccggtcc cgtgcccgtc gaacggccac cacgtctact 1200

.......... ........ ORF2 ORF2 EGFP ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact 1260

1201 tgaagtccca gtcgaacggc atccaccgta gcgggagcgg gagcggcctg tgcgacttga 1260

.......... ........ ORF2 ORF2 EGFP ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct 1320

1261 acaccggcaa atgcagcggc aggtcgagct ggtcctaccc gtggtggggc cacttgtcga 1320

.......... . ORF2 ORF2 EGFP EGFP_N_primer . .......... ..........

.......... .......... AgeI(1347) .......... .......... ..........

1321 cctcgccctt gctcaccatg gtggcgaccg gtagcgctag cggatctgac ggttcactaa 1380

1321 ggagcgggaa cgagtggtac caccgctggc catcgcgatc gcctagactg ccaagtgatt 1380

ORF2 EGFP ORF2 .......... .......... ......... CMV_promoter

.......... .......... .......... .......... .......... ..........

1381 accagctctg cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg 1440

1381 tggtcgagac gaatatatct ggagggtggc atgtgcggat ggcgggtaaa cgcagttacc 1440

.......... . CMV_promoter CMV_immearly_promoter CMV_fwd_primer .

.......... .......... .......... .......... .......... ..........

1441 ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc 1500

1441 ccgcctcaac aatgctgtaa aacctttcag ggcaactaaa accacggttt tgtttgaggg 1500

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc 1560

1501 taactgcagt taccccacct ctgaaccttt aggggcactc agtttggcga taggtgcggg 1560

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta 1620

1561 taactacatg acggttttgg cgtagtggta ccattatcgc tactgattat g catctacat 1620

.......... .... CMV_immearly_promoter ... CAG_enhancer .........

.......... .......... .......... .......... .......... ..........

1621 ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt 1680

1621 gacggttcat cctttcaggg tattccagta catgacccgt attacggtcc gcccggtaaa 1680

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... NdeI(1715) .......... ..........

1681 accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa 1740

1681 tggcagtaac tgcagttatc ccccgcatga accgtatact atgtgaacta catgacggtt 1740

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

1741 gtgggcagtt taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt 1800

1741 cacccgtcaa atggcattta tgaggtgggt aactgcagtt acctttcagg gataaccgca 1800

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

1801 tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc 1860

1801 atgataccct tgtatgcagt aataactgca gttacccgcc cccagcaacc cgccagtcgg 1860

........ CMV_immearly_promoter CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

1861 aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa 1920

1861 tccgcccggt aaatggcatt caatacattg cgccttgagg tatatacccg atacttgatt 1920

... CAG_enhancer CMV_immearly_promoter ... .......... ..........

.......... .......... ... BglII(1953) KpnI(1963),SalI(1965) ...

1921 tgaccccgta attgattact attannncta gcagatctgg taccgtcgac gcggccgcga 1980

1921 actggggcat taactaatga taatnnngat cgtctagacc atggcagctg cgccggcgct 1980

CMV_immearly_promoter ........ .......... .......... ..........

EcoRV(1981),XhoI(1985) HindIII(1991) .... .......... ..........

1981 tatcctcgag aagctttcta gagnnntaag ggtgggaaag aatatataag gtgggggtct 2040

1981 ataggagctc ttcgaaagat ctcnnnattc ccaccctttc ttatatattc cacccccaga 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatgtagttt tgtatctgtt ttgcagcagc cgccgccgcc atgagcacca actcgtttga 2100

2041 atacatcaaa acatagacaa aacgtcgtcg gcggcggcgg tactcgtggt tgagcaaact 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tggaagcatt gtgagctcat atttgacaac gcgcatgccc ccatgggccg gggtgcgtca 2160

2101 accttcgtaa cactcgagta taaactgttg cgcgtacggg ggtacccggc cccacgcagt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gaatgtgatg ggctccagca ttgatggtcg ccccgtcctg cccgcaaact ctactacctt 2220

2161 cttacactac ccgaggtcgt aactaccagc ggggcaggac gggcgtttga gatgatggaa 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gacctacgag accgtgtctg gaacgccgtt ggagactgca gcctccgccg ccgcttcagc 2280

2221 ctggatgctc tggcacagac cttgcggcaa cctctgacgt cggaggcggc ggcgaagtcg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgctgcagcc accgcccgcg ggattgtgac tgactttgct ttcctgagcc cgcttgcaag 2340

2281 gcgacgtcgg tggcgggcgc cctaacactg actgaaacga aaggactcgg gcgaacgttc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cagtgcagct tcccgttcat ccgcccgcga tgacaagttg acggctcttt tggcacaatt 2400

2341 gtcacgtcga agggcaagta ggcgggcgct actgttcaac tgccgagaaa accgtgttaa 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggattctttg acccgggaac ttaatgtcgt ttctcagcag ctgttggatc tgcgccagca 2460

2401 cctaagaaac tgggcccttg aattacagca aagagtcgtc gacaacctag acgcggtcgt 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggtttctgcc ctgaaggctt cctcccctcc caatgcggtt taaaacataa ataaaaaacc 2520

2461 ccaaagacgg gacttccgaa ggaggggagg gttacgccaa attttgtatt tattttttgg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agactctgtt tggatttgga tcaagcaagt gtcttgctgt ctttatttag gggttttgcg 2580

2521 tctgagacaa acctaaacct agttcgttca cagaacgaca gaaataaatc cccaaaacgc 2580

.......... .......... .......... .......... ......... Orf frame 1

.......... .......... .......... .......... .......... ..........

2581 cgcgcggtag gcccgggacc agcggtctcg gtcgttgagg gtcctgtgta ttttttccag 2640

2581 gcgcgccatc cgggccctgg tcgccagagc cagcaactcc caggacacat aaaaaaggtc 2640

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gacgtggtaa aggtgactct ggatgttcag atacatgggc ataagcccgt ctctggggtg 2700

2641 ctgcaccatt tccactgaga cctacaagtc tatgtacccg tattcgggca gagaccccac 2700

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaggtagcac cactgcagag cttcatgctg cggggtggtg ttgtagatga tccagtcgta 2760

2701 ctccatcgtg gtgacgtctc gaagtacgac gccccaccac aacatctact aggtcagcat 2760

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcaggagcgc tgggcgtggt gcctaaaaat gtctttcagt agcaagctga ttgccagggg 2820

2761 cgtcctcgcg acccgcacca cggattttta cagaaagtca tcgttcgact aacggtcccc 2820

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 caggcccttg gtgtaagtgt ttacaaagcg gttaagctgg gatgggtgca tacgtgggga 2880

2821 gtccgggaac cacattcaca aatgtttcgc caattcgacc ctacccacgt atgcacccct 2880

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tatgagatgc atcttggact gtatttttag gttggctatg ttcccagcca tatccctccg 2940

2881 atactctacg tagaacctga cataaaaatc caaccgatac aagggtcggt atagggaggc 2940

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gggattcatg ttgtgcagaa ccaccagcac agtgtatccg gtgcacttgg gaaatttgtc 3000

2941 ccctaagtac aacacgtctt ggtggtcgtg tcacataggc cacgtgaacc ctttaaacag 3000

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 atgtagctta gaaggaaatg cgtggaagaa cttggagacg cccttgtgac ctccaagatt 3060

3001 tacatcgaat cttcctttac gcaccttctt gaacctctgc gggaacactg gaggttctaa 3060

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttccatgcat tcgtccataa tgatggcaat gggcccacgg gcggcggcct gggcgaagat 3120

3061 aaggtacgta agcaggtatt actaccgtta cccgggtgcc cgccgccgga cccgcttcta 3120

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 atttctggga tcactaacgt catagttgtg ttccaggatg agatcgtcat aggccatttt 3180

3121 taaagaccct agtgattgca gtatcaacac aaggtcctac tctagcagta tccggtaaaa 3180

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tacaaagcgc gggcggaggg tgccagactg cggtataatg gttccatccg gcccaggggc 3240

3181 atgtttcgcg cccgcctccc acggtctgac gccatattac caaggtaggc cgggtccccg 3240

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtagttaccc tcacagattt gcatttccca cgctttgagt tcagatgggg ggatcatgtc 3300

3241 catcaatggg agtgtctaaa cgtaaagggt gcgaaactca agtctacccc cctagtacag 3300

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tacctgcggg gcgatgaaga aaacggtttc cggggtaggg gagatcagct gggaagaaag 3360

3301 atggacgccc cgctacttct tttgccaaag gccccatccc ctctagtcga cccttctttc 3360

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 caggttcctg agcagctgcg acttaccgca gccggtgggc ccgtaaatca cacctattac 3420

3361 gtccaaggac tcgtcgacgc tgaatggcgt cggccacccg ggcatttagt gtggataatg 3420

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgggtgcaac tggtagttaa gagagctgca gctgccgtca tccctgagca ggggggccac 3480

3421 gcccacgttg accatcaatt ctctcgacgt cgacggcagt agggactcgt ccccccggtg 3480

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttcgttaagc atgtccctga ctcgcatgtt ttccctgacc aaatccgcca gaaggcgctc 3540

3481 aagcaattcg tacagggact gagcgtacaa aagggactgg tttaggcggt cttccgcgag 3540

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gccgcccagc gatagcagtt cttgcaagga agcaaagttt ttcaacggtt tgagaccgtc 3600

3541 cggcgggtcg ctatcgtcaa gaacgttcct tcgtttcaaa aagttgccaa actctggcag 3600

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgccgtaggc atgcttttga gcgtttgacc aagcagttcc aggcggtccc acagctcggt 3660

3601 gcggcatccg tacgaaaact cgcaaactgg ttcgtcaagg tccgccaggg tgtcgagcca 3660

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cacctgctct acggcatctc gatccagcat atctcctcgt ttcgcgggtt ggggcggctt 3720

3661 gtggacgaga tgccgtagag ctaggtcgta tagaggagca aagcgcccaa ccccgccgaa 3720

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcgctgtacg gcagtagtcg gtgctcgtcc agacgggcca gggtcatgtc tttccacggg 3780

3721 agcgacatgc cgtcatcagc cacgagcagg tctgcccggt cccagtacag aaaggtgccc 3780

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgcagggtcc tcgtcagcgt agtctgggtc acggtgaagg ggtgcgctcc gggctgcgcg 3840

3781 gcgtcccagg agcagtcgca tcagacccag tgccacttcc ccacgcgagg cccgacgcgc 3840

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctggccaggg tgcgcttgag gctggtcctg ctggtgctga agcgctgccg gtcttcgccc 3900

3841 gaccggtccc acgcgaactc cgaccaggac gaccacgact tcgcgacggc cagaagcggg 3900

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgcgcgtcgg ccaggtagca tttgaccatg gtgtcatagt ccagcccctc cgcggcgtgg 3960

3901 acgcgcagcc ggtccatcgt aaactggtac cacagtatca ggtcggggag gcgccgcacc 3960

......... Orf frame 1 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cccttggcgc gcagcttgcc cttggaggag gcgccgcacg aggggcagtg cagacttttg 4020

3961 gggaaccgcg cgtcgaacgg gaacctcctc cgcggcgtgc tccccgtcac gtctgaaaac 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agggcgtaga gcttgggcgc gagaaatacc gattccgggg agtaggcatc cgcgccgcag 4080

4021 tcccgcatct cgaacccgcg ctctttatgg ctaaggcccc tcatccgtag gcgcggcgtc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gccccgcaga cggtctcgca ttccacgagc caggtgagct ctggccgttc ggggtcaaaa 4140

4081 cggggcgtct gccagagcgt aaggtgctcg gtccactcga gaccggcaag ccccagtttt 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 accaggtttc ccccatgctt tttgatgcgt ttcttacctc tggtttccat gagccggtgt 4200

4141 tggtccaaag ggggtacgaa aaactacgca aagaatggag accaaaggta ctcggccaca 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EcoRI(4258)

4201 ccacgctcgg tgacgaaaag gctgtccgtg tccccgtata cagactnnng tttaaacgaa 4260

4201 ggtgcgagcc actgcttttc cgacaggcac aggggcatat gtctgannnc aaatttgctt 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ttcnnntata aaatgcaagg tgctgctcaa aaaatcaggc aaagcctcgc gcaaaaaaga 4320

4261 aagnnnatat tttacgttcc acgacgagtt ttttagtccg tttcggagcg cgttttttct 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aagcacatcg tagtcatgct catgcagata aaggcaggta agctccggaa ccaccacaga 4380

4321 ttcgtgtagc atcagtacga gtacgtctat ttccgtccat tcgaggcctt ggtggtgtct 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aaaagacacc atttttctct caaacatgtc tgcgggtttc tgcataaaca caaaataaaa 4440

4381 ttttctgtgg taaaaagaga gtttgtacag acgcccaaag acgtatttgt gttttatttt 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 taacaaaaaa acatttaaac attagaagcc tgtcttacaa caggaaaaac aacccttata 4500

4441 attgtttttt tgtaaatttg taatcttcgg acagaatgtt gtcctttttg ttgggaatat 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 agcataagac ggactacggc catgccggcg tgaccgtaaa aaaactggtc accgtgatta 4560

4501 tcgtattctg cctgatgccg gtacggccgc actggcattt ttttgaccag tggcactaat 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aaaagcacca ccgacagctc ctcggtcatg tccggagtca taatgtaaga ctcggtaaac 4620

4561 ttttcgtggt ggctgtcgag gagccagtac aggcctcagt attacattct gagccatttg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acatcaggtt gattcatcgg tcagtgctaa aaagcgaccg aaatagcccg ggggaataca 4680

4621 tgtagtccaa ctaagtagcc agtcacgatt tttcgctggc tttatcgggc ccccttatgt 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tacccgcagg cgtagagaca acattacagc ccccatagga ggtataacaa aattaatagg 4740

4681 atgggcgtcc gcatctctgt tgtaatgtcg ggggtatcct ccatattgtt ttaattatcc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agagaaaaac acataaacac ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg 4800

4741 tctctttttg tgtatttgtg gactttttgg gaggacggat ccgttttatc gtgggagggc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctccagaaca acatacagcg cttcacagcg gcagcctaac agtcagcctt accagtaaaa 4860

4801 gaggtcttgt tgtatgtcgc gaagtgtcgc cgtcggattg tcagtcggaa tggtcatttt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aagaaaacct attaaaaaaa caccactcga cacggcacca gctcaatcag tcacagtgta 4920

4861 ttcttttgga taattttttt gtggtgagct gtgccgtggt cgagttagtc agtgtcacat 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaaaagggcc aagtgcagag cgagtatata taggactaaa aaatgacgta acggttaaag 4980

4921 tttttcccgg ttcacgtctc gctcatatat atcctgattt tttactgcat tgccaatttc 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tccacaaaaa acacccagaa aaccgcacgc gaacctacgc ccagaaacga aagccaaaaa 5040

4981 aggtgttttt tgtgggtctt ttggcgtgcg cttggatgcg ggtctttgct ttcggttttt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 acccacaact tcctcaaatc gtcacttccg ttttcccacg ttacgtaact tcccatttta 5100

5041 tgggtgttga aggagtttag cagtgaaggc aaaagggtgc aatgcattga agggtaaaat 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 agaaaactac aattcccaac acatacaagt tactccgccc taaaacctac gtcacccgcc 5160

5101 tcttttgatg ttaagggttg tgtatgttca atgaggcggg attttggatg cagtgggcgg 5160

.......... .......... .......... .......... .......... LITR RITR

.......... .......... .......... .......... .......... ..........

5161 ccgttcccac gccccgcgcc acgtcacaaa ctccaccccc tcattatcat attggcttca 5220

5161 ggcaagggtg cggggcgcgg tgcagtgttt gaggtggggg agtaatagta taaccgaagt 5220

.......... .......... LITR RITR .......... .......... ..........

.......... .......... ......... BamHI(5260) ......... ..........

5221 atccaaaata aggtatatta ttgatgatnn nttaattaag gatccnnncg gtgtgaaata 5280

5221 taggttttat tccatataat aactactann naattaattc ctaggnnngc cacactttat 5280

.......... LITR RITR .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact 5340

5281 ggcgtgtcta cgcattcctc ttttatggcg tagtccgcga gaaggcgaag gagcgagtga 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 5400

5341 ctgagcgacg cgagccagca agccgacgcc gctcgccata gtcgagtgag tttccgccat 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 5460

5401 tatgccaata ggtgtcttag tcccctattg cgtcctttct tgtacactcg ttttccggtc 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 5520

5461 gttttccggt ccttggcatt tttccggcgc aacgaccgca aaaaggtatc cgaggcgggg 5520

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 5580

5521 ggactgctcg tagtgttttt agctgcgagt tcagtctcca ccgctttggg ctgtcctgat 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 5640

5581 atttctatgg tccgcaaagg gggaccttcg agggagcacg cgagaggaca aggctgggac 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 5700

5641 ggcgaatggc ctatggacag gcggaaagag ggaagccctt cgcaccgcga aagagtatcg 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 5760

5701 agtgcgacat ccatagagtc aagccacatc cagcaagcga ggttcgaccc gacacacgtg 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 5820

5761 cttggggggc aagtcgggct ggcgacgcgg aataggccat tgatagcaga actcaggttg 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 5880

5821 ggccattctg tgctgaatag cggtgaccgt cgtcggtgac cattgtccta atcgtctcgc 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 5940

5881 tccatacatc cgccacgatg tctcaagaac ttcaccaccg gattgatgcc gatgtgatct 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 6000

5941 tcctgtcata aaccatagac gcgagacgac ttcggtcaat ggaagccttt ttctcaacca 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 6060

6001 tcgagaacta ggccgtttgt ttggtggcga ccatcgccac caaaaaaaca aacgttcgtc 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 6120

6061 gtctaatgcg cgtctttttt tcctagagtt cttctaggaa actagaaaag atgccccaga 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 6180

6121 ctgcgagtca ccttgctttt gagtgcaatt ccctaaaacc agtactctaa tagtttttcc 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 6240

6181 tagaagtgga tctaggaaaa tttaattttt acttcaaaat ttagttagat ttcatatata 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 6300

6241 ctcatttgaa ccagactgtc aatggttacg aattagtcac tccgtggata gagtcgctag 6300

.......... .......... .......... Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

6301 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 6360

6301 acagataaag caagtaggta tcaacggact gaggggcagc acatctattg atgctatgcc 6360

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 6420

6361 ctcccgaatg gtagaccggg gtcacgacgt tactatggcg ctctgggtgc gagtggccga 6420

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 6480

6421 ggtctaaata gtcgttattt ggtcggtcgg ccttcccggc tcgcgtcttc accaggacgt 6480

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 6540

6481 tgaaataggc ggaggtaggt cagataatta acaacggccc ttcgatctca ttcatcaagc 6540

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ccagttaata gtttgcgcaa cgttgttgnn nnnnaaaaag gatcttcacc tagatccttt 6600

6541 ggtcaattat caaacgcgtt gcaacaacnn nnnntttttc ctagaagtgg atctaggaaa 6600

Ampicillin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg 6660

6601 agtgcatctt tcggtcaggc gtctttgcca cgactggggc ctacttacag tcgatgaccc 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta 6720

6661 gatagacctg ttcccttttg cgttcgcgtt tctctttcgt ccatcgaacg tcacccgaat 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg 6780

6721 gtaccgctat cgatctgacc cgccaaaata cctgtcgttc gcttggcctt aacggtcgac 6780

.......... .......... .......... .......... ....... NEOKAN_promoter

.......... .......... .......... .......... .......... ..........

6781 gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttctcgccgc 6840

6781 cccgcgggag accattccaa cccttcggga cgtttcattt gacctaccga aagagcggcg 6840

....... NEOKAN_promoter ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga ggatcgtttc 6900

6841 gttcctagac taccgcgtcc cctagttcga gactagttct ctgtcctact cctagcaaag 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat 6960

6901 cgtactaact tgttctacct aacgtgcgtc caagaggccg gcgaacccac ctctccgata 6960

.......... .......... ........ ORF3 Neomycin ........ ..........

.......... .......... .......... .......... .......... ..........

6961 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt 7020

6961 agccgatact gacccgtgtt gtctgttagc cgacgagact acggcggcac aaggccgaca 7020

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7021 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac 7080

7021 gtcgcgtccc cgcgggccaa gaaaaacagt tctggctgga caggccacgg gacttacttg 7080

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7081 tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg 7140

7081 acgttctgct ccgtcgcgcc gatagcaccg accggtgctg cccgcaagga acgcgtcgac 7140

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7141 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc 7200

7141 acgagctgca acagtgactt cgcccttccc tgaccgacga taacccgctt cacggccccg 7200

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7201 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa 7260

7201 tcctagagga cagtagagtg gaacgaggac ggctctttca taggtagtac cgactacgtt 7260

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc 7320

7261 acgccgccga cgtatgcgaa ctaggccgat ggacgggtaa gctggtggtt cgctttgtag 7320

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7321 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg 7380

7321 cgtagctcgc tcgtgcatga gcctaccttc ggccagaaca gctagtccta ctagacctgc 7380

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7381 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg 7440

7381 ttctcgtagt ccccgagcgc ggtcggcttg acaagcggtc cgagttccgc tcgtacgggc 7440

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7441 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa 7500

7441 tgccgctcct agagcagcac tgggtaccgc tacggacgaa cggcttatag taccaccttt 7500

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7501 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg 7560

7501 taccggcgaa aagacctaag tagctgacac cggccgaccc acaccgcctg gcgatagtcc 7560

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7561 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct 7620

7561 tgtatcgcaa ccgatgggca ctataacgac ttctcgaacc gccgcttacc cgactggcga 7620

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7621 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc 7680

7621 aggagcacga aatgccatag cggcgagggc taagcgtcgc gtagcggaag atagcggaag 7680

.......... ........ ORF3 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7681 ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tcagctcatt ttttaaccaa 7740

7681 aactgctcaa gaagacttaa aacaatttta aaaacaattt agtcgagtaa aaaattggtt 7740

ORF3 Neomycin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 taggccgaaa tcggcaacat cccttataaa tcaaaagaat agaccgcgat agggttgagt 7800

7741 atccggcttt agccgttgta gggaatattt agttttctta tctggcgcta tcccaactca 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg 7860

7801 caacaaggtc aaaccttgtt ctcaggtgat aatttcttgc acctgaggtt gcagtttccc 7860

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

7861 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa atcaagtttt 7920

7861 gctttttggc agatagtccc gctaccgggt gatgcacttg gtagtgggtt tagttcaaaa 7920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ttgcggtcga ggtgccgtaa agctctaaat cggaacccta aagggagccc ccgatttaga 7980

7921 aacgccagct ccacggcatt tcgagattta gccttgggat ttccctcggg ggctaaatct 7980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg 8040

7981 cgaactgccc ctttcggccg cttgcaccgc tctttccttc ccttctttcg ctttcctcgc 8040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgcgcgc 8100

8041 ccgcgatccc gcgaccgttc acatcgccag tgcgacgcgc attggtggtg tgggcgcgcg 8100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 ttaatgcgcc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8160

8101 aattacgcgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 nnnnnnnnnn nnnnn..... .......... .......... .......... .......... 8175

8161 nnnnnnnnnn nnnnn..... .......... .......... .......... .......... 8175

.......... .......... .......... .......... .......... ..........