Sequence of pAmCyan1-N1

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Sequence of pAmCyan1-N1

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Sequence of pAmCyan1-N1 : 4703 BP; 1115 A; 1295 C; 1229 G; 1064 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ....... CMV_immearly_pro .. CMV_fwd_pri CMV_pro .....

.......... .......... .......... .......... .......... ..........

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

BglII,XhoI SacI,HindIII(622) EcoRI(629) SalI(639)

601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 660

601 ggcctgagtc tagagctcga gttcgaagct taagacgtca gctgccatgg cgcccgggcc 660

.......... .......... .......... .......... .......... ..........

AgeI(666) .......... .......... .......... .......... ..........

661 gatccaccgg tcgccaccat ggccctgtcc aacaagttca tcggcgacga catgaagatg 720

661 ctaggtggcc agcggtggta ccgggacagg ttgttcaagt agccgctgct gtacttctac 720

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

721 acctaccaca tggacggctg cgtgaacggc cactacttca ccgtgaaggg cgagggcagc 780

721 tggatggtgt acctgccgac gcacttgccg gtgatgaagt ggcacttccc gctcccgtcg 780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggcaagccct acgagggcac ccagacctcc accttcaagg tgaccatggc caacggcggc 840

781 ccgttcggga tgctcccgtg ggtctggagg tggaagttcc actggtaccg gttgccgccg 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cccctggcct tctccttcga catcctgtcc accgtgttca tgtacggcaa ccgctgcttc 900

841 ggggaccgga agaggaagct gtaggacagg tggcacaagt acatgccgtt ggcgacgaag 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 accgcctacc ccaccagcat gcccgactac ttcaagcagg ccttccccga cggcatgtcc 960

901 tggcggatgg ggtggtcgta cgggctgatg aagttcgtcc ggaaggggct gccgtacagg 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tacgagagaa ccttcaccta cgaggacggc ggcgtggcca ccgccagctg ggagatcagc 1020

961 atgctctctt ggaagtggat gctcctgccg ccgcaccggt ggcggtcgac cctctagtcg 1020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctgaagggca actgcttcga gcacaagtcc accttccacg gcgtgaactt ccccgccgac 1080

1021 gacttcccgt tgacgaagct cgtgttcagg tggaaggtgc cgcacttgaa ggggcggctg 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggccccgtga tggccaagaa gaccaccggc tgggacccct ccttcgagaa gatgaccgtg 1140

1081 ccggggcact accggttctt ctggtggccg accctgggga ggaagctctt ctactggcac 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgcgacggca tcttgaaggg cgacgtgacc gccttcctga tgctgcaggg cggcggcaac 1200

1141 acgctgccgt agaacttccc gctgcactgg cggaaggact acgacgtccc gccgccgttg 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tacagatgcc agttccacac ctcctacaag accaagaagc ccgtgaccat gccccccaac 1260

1201 atgtctacgg tcaaggtgtg gaggatgttc tggttcttcg ggcactggta cggggggttg 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cacgtggtgg agcaccgcat cgccagaacc gacctggaca agggcggcaa cagcgtgcag 1320

1261 gtgcaccacc tcgtggcgta gcggtcttgg ctggacctgt tcccgccgtt gtcgcacgtc 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctgaccgagc acgccgtggc ccacatcacc tccgtggtgc ccttctgaag cggccgcgac 1380

1321 gactggctcg tgcggcaccg ggtgtagtgg aggcaccacg ggaagacttc gccggcgctg 1380

.......... .......... ORF 1 .......... .......... ..........

XbaI(1381) .......... .......... .......... .......... ..........

1381 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc 1440

1381 agatctagta ttagtcggta tggtgtaaac atctccaaaa tgaacgaaat tttttggagg 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta 1500

1441 gtgtggaggg ggacttggac tttgtatttt acttacgtta acaacaacaa ttgaacaaat 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 1560

1501 aacgtcgaat attaccaatg tttatttcgt tatcgtagtg tttaaagtgt ttatttcgta 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... AflII(1609) .........

1561 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa 1620

1561 aaaaaagtga cgtaagatca acaccaaaca ggtttgagta gttacataga attccgcatt 1620

.......... .......... ......... EBV_rev_pri ......... ..........

.......... .......... .......... .......... .......... ..........

1621 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt 1680

1621 taacattcgc aattataaaa caattttaag cgcaatttaa aaacaattta gtcgagtaaa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata 1740

1681 aaattggtta tccggcttta gccgttttag ggaatattta gttttcttat ctggctctat 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac 1800

1741 cccaactcac aacaaggtca aaccttgttc tcaggtgata atttcttgca cctgaggttg 1800

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa 1860

1801 cagtttcccg ctttttggca gatagtcccg ctaccgggtg atgcacttgg tagtgggatt 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc 1920

1861 agttcaaaaa accccagctc cacggcattt cgtgatttag ccttgggatt tccctcgggg 1920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg 1980

1921 gctaaatctc gaactgcccc tttcggccgc ttgcaccgct ctttccttcc cttctttcgc 1980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca 2040

1981 tttcctcgcc cgcgatcccg cgaccgttca catcgccagt gcgacgcgca ttggtggtgt 2040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg 2100

2041 gggcggcgcg aattacgcgg cgatgtcccg cgcagtccac cgtgaaaagc ccctttacac 2100

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga 2160

2101 gcgccttggg gataaacaaa taaaaagatt tatgtaagtt tatacatagg cgagtactct 2160

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

2161 caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga 2220

2161 gttattggga ctatttacga agttattata actttttcct tctcaggact ccgcctttct 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 2280

2221 tggtcgacac cttacacaca gtcaatccca cacctttcag gggtccgagg ggtcgtccgt 2280

......... pBABE_3_pri ......... SV40_enh SV40_pro ..........

.......... .......... .......... .......... .......... ..........

2281 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 2340

2281 cttcatacgt ttcgtacgta gagttaatca gtcgttggtc cacacctttc aggggtccga 2340

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 2400

2341 ggggtcgtcc gtcttcatac gtttcgtacg tagagttaat cagtcgttgg tatcagggcg 2400

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 2460

2401 gggattgagg cgggtagggc ggggattgag gcgggtcaag gcgggtaaga ggcggggtac 2460

.......... ..... SV40_enh SV40_pro SV40_origin ..... ..........

.......... .......... .......... .......... .......... ..........

2461 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 2520

2461 cgactgatta aaaaaaataa atacgtctcc ggctccggcg gagccggaga ctcgataagg 2520

... SV40_origin SV40_pro SV40pro_F_pri ... .......... ..........

.......... .......... .......... .......... ClaI(2567) ..........

2521 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca 2580

2521 tcttcatcac tcctccgaaa aaacctccgg atccgaaaac gtttctagct agttctctgt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc t ccggccgct 2640

2581 cctactccta gcaaagcgta ctaacttgtt ctacctaacg tgcgtccaag aggccggcga 2640

.......... .......... .......... ORF 1 Neomycin ..........

.......... .......... .......... .......... .......... ..........

2641 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc 2700

2641 acccacctct ccgataagcc gatactgacc cgtgttgtct gttagccgac gagactacgg 2700

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc 2760

2701 cggcacaagg ccgacagtcg cgtccccgcg ggccaagaaa aacagttctg gctggacagg 2760

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc 2820

2761 ccacgggact tacttgacgt tctgctccgt cgcgccgata gcaccgaccg gtgctgcccg 2820

.......... ........ ORF 1 Neomycin ........ .......... ..........

FspI(2829) .......... .......... .......... .......... ..........

2821 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg 2880

2821 caaggaacgc gtcgacacga gctgcaacag tgacttcgcc cttccctgac cgacgataac 2880

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc 2940

2881 ccgcttcacg gccccgtcct agaggacagt agagtggaac gaggacggct ctttcatagg 2940

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac 3000

2941 tagtaccgac tacgttacgc cgccgacgta tgcgaactag gccgatggac gggtaagctg 3000

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat 3060

3001 gtggttcgct ttgtagcgta gctcgctcgt gcatgagcct accttcggcc agaacagcta 3060

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc 3120

3061 gtcctactag acctgcttct cgtagtcccc gagcgcggtc ggcttgacaa gcggtccgag 3120

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg 3180

3121 ttccgctcgt acgggctgcc gctcctagag cagcactggg taccgctacg gacgaacggc 3180

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg 3240

3181 ttatagtacc accttttacc ggcgaaaaga cctaagtagc tgacaccggc cgacccacac 3240

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc 3300

3241 cgcctggcga tagtcctgta tcgcaaccga tgggcactat aacgacttct cgaaccgccg 3300

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc 3360

3301 cttacccgac tggcgaagga gcacgaaatg ccatagcggc gagggctaag cgtcgcgtag 3360

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg 3420

3361 cggaagatag cggaagaact gctcaagaag actcgccctg agaccccaag ctttactggc 3420

........ ORF 1 Neomycin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 3480

3421 tggttcgctg cgggttggac ggtagtgctc taaagctaag gtggcggcgg aagatacttt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 3540

3481 ccaacccgaa gccttagcaa aaggccctgc ggccgaccta ctaggaggtc gcgcccctag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa 3600

3541 agtacgacct caagaagcgg gtgggatccc cctccgattg actttgtgcc ttcctctgtt 3600

.......... .......... .......... .......... TK_PA_term ..........

.......... .......... .......... .......... .......... ..........

3601 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg 3660

3601 atggccttcc ttgggcgcga tactgccgtt atttttctgt cttattttgc gtgccacaac 3660

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac 3720

3661 ccagcaaaca agtatttgcg ccccaagcca gggtcccgac cgtgagacag ctatggggtg 3720

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa 3780

3721 gctctggggt aaccccggtt atgcgggcgc aaagaaggaa aaggggtggg gtggggggtt 3780

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct 3840

3781 caagcccact tccgggtccc gagcgtcggt tgcagccccg ccgtccggga cggtatcgga 3840

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 3900

3841 gtccaatgag tatatatgaa atctaactaa attttgaagt aaaaattaaa ttttcctaga 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 3960

3901 tccacttcta ggaaaaacta ttagagtact ggttttaggg aattgcactc aaaagcaagg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 4020

3961 tgactcgcag tctggggcat cttttctagt ttcctagaag aactctagga aaaaaagacg 4020

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4021 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 4080

4021 cgcattagac gacgaacgtt tgtttttttg gtggcgatgg tcgccaccaa acaaacggcc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 4140

4081 tagttctcga tggttgagaa aaaggcttcc attgaccgaa gtcgtctcgc gtctatggtt 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 4200

4141 tatgacagga agatcacatc ggcatcaatc cggtggtgaa gttcttgaga catcgtggcg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 4260

4201 gatgtatgga gcgagacgat taggacaatg gtcaccgacg acggtcaccg ctattcagca 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 4320

4261 cagaatggcc caacctgagt tctgctatca atggcctatt ccgcgtcgcc agcccgactt 4320

.......... ........ pBR322_origin ........ .......... ..........

......... ApaLI(4331) ......... .......... .......... ..........

4321 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 4380

4321 gccccccaag cacgtgtgtc gggtcgaacc tcgcttgctg gatgtggctt gactctatgg 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 4440

4381 atgtcgcact cgatactctt tcgcggtgcg aagggcttcc ctctttccgc ctgtccatag 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 4500

4441 gccattcgcc gtcccagcct tgtcctctcg cgtgctccct cgaaggtccc cctttgcgga 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 4560

4501 ccatagaaat atcaggacag cccaaagcgg tggagactga actcgcagct aaaaacacta 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 4620

4561 cgagcagtcc ccccgcctcg gatacctttt tgcggtcgtt gcgccggaaa aatgccaagg 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 4680

4621 accggaaaac gaccggaaaa cgagtgtaca agaaaggacg caatagggga ctaagacacc 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ataaccgtat taccgccatg cat....... .......... .......... .......... 4703

4681 tattggcata atggcggtac gta....... .......... .......... .......... 4703

.......... .......... .......... .......... .......... ..........