Sequence of pAmCyan

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Sequence of pAmCyan

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Sequence of pAmCyan : 3325 BP; 848 A; 802 C; 852 G; 823 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

1 tcgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

61 tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cactcaatcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

121 agtgagtaat ccgtggggtc cgaaatgtga aatacgaagg ccgagcatac aacacacctt 180

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gc caagcttg 240

181 aacactcgcc tattgttaaa gtgtgtcctt tgtcgatact ggtactaatg cggttcgaac 240

....... M13_pUC_rev_pri .... M13_reverse_pri ORF 1 .......

PstI250),SalI(252) BamHI(264) SmaI(271) KpnI(277) ..........

241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggctctttca 300

241 gtacggacgt ccagctgaga tctcctaggg gcccatggcc agcggtggta ccgagaaagt 300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aacaagttta tcggagatga catgaaaatg acctaccata tggatggctg tgtcaatggg 360

301 ttgttcaaat agcctctact gtacttttac tggatggtat acctaccgac acagttaccc 360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cattacttta ccgtcaaagg tgaaggcagc gggaagccat acgaagggac gcagacctcg 420

361 gtaatgaaat ggcagtttcc acttccgtcg cccttcggta tgcttccctg cgtctggagc 420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 acttttaaag tcaccatggc caacggtggg ccccttgcat tctcctttga catactatct 480

421 tgaaaatttc agtggtaccg gttgccaccc ggggaacgta agaggaaact gtatgataga 480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... ClaI(501) .......... .......... ..........

481 acagtgttca tgtatggaaa tcgatgcttt actgcgtatc ctaccagtat gcccgactat 540

481 tgtcacaagt acataccttt agctacgaaa tgacgcatag gatggtcata cgggctgata 540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ttcaaacaag catttcctga cggaatgtca tatgaaagga cttttaccta tgaagatgga 600

541 aagtttgttc gtaaaggact gccttacagt atactttcct gaaaatggat acttctacct 600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggagttgcta cagccagttg ggaaataagc cttaaaggca actgctttga gcacaaatcc 660

601 cctcaacgat gtcggtcaac cctttattcg gaatttccgt tgacgaaact cgtgtttagg 660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acgtttcatg gagtgaactt tcctgctgat ggacctgtga tggcgaagat gacaactggt 720

661 tgcaaagtac ctcacttgaa aggacgacta cctggacact accgcttcta ctgttgacca 720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgggacccat cttttgagaa aatgactgtc tgcgatggaa tattgaaggg tgatgtcacc 780

721 accctgggta gaaaactctt ttactgacag acgctacctt ataacttccc actacagtgg 780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gcgttcctca tgctgcaagg aggtggcaat tacagatgcc aattccacac ttcttacaag 840

781 cgcaaggagt acgacgttcc tccaccgtta atgtctacgg ttaaggtgtg aagaatgttc 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 acaaaaaaac cggtgacgat gccaccaaac catgcggtgg aacatcgcat tgcgaggacc 900

841 tgtttttttg gccactgcta cggtggtttg gtacgccacc ttgtagcgta acgctcctgg 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gaccttgaca aaggtggcaa cagtgttcag ctgacggagc acgctgttgc acatataacc 960

901 ctggaactgt ttccaccgtt gtcacaagtc gactgcctcg tgcgacaacg tgtatattgg 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... NotI(981) .......... .......... ..........

961 tctgttgtcc ctttctgaag cggccgcgac tctagaattc caactgagcg ccggtcgcta 1020

961 agacaacagg gaaagacttc gccggcgctg agatcttaag gttgactcgc ggccagcgat 1020

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .... StuI(1055),SpeI(1059) .... ..........

1021 ccattaccaa cttgtctggt gtcaaaaata ataggcctac tagtcggccg tacgggccct 1080

1021 ggtaatggtt gaacagacca cagtttttat tatccggatg atcagccggc atgcccggga 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 1140

1081 aagcagagcg cgcaaagcca ctactgccac ttttggagac tgtgtacgtc gagggcctct 1140

.......... .......... .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

1141 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 1200

1141 gccagtgtcg aacagacatt cgcctacggc cctcgtctgt tcgggcagtc ccgcgcagtc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 1260

1201 gcccacaacc gcccacagcc ccgaccgaat tgatacgccg tagtctcgtc taacatgact 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 1320

1261 ctcacgtggt atacgccaca ctttatggcg tgtctacgca ttcctctttt atggcgtagt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggcggcctta agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg 1380

1321 ccgccggaat tcccggagca ctatgcggat aaaaatatcc aattacagta ctattattac 1380

.......... .......... .......... .......... .......... ..........

......... AatII(1393) ......... .......... .......... ..........

1381 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta 1440

1381 caaagaatct gcagtccacc gtgaaaagcc cctttacacg cgccttgggg ataaacaaat 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 1500

1441 aaaaagattt atgtaagttt atacataggc gagtactctg ttattgggac tatttacgaa 1500

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 1560

1501 gttattataa ctttttcctt ctcatactca taagttgtaa aggcacagcg ggaataaggg 1560

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

1561 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 1620

1561 aaaaaacgcc gtaaaacgga aggacaaaaa cgagtgggtc tttgcgacca ctttcatttt 1620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 1680

1621 ctacgacttc tagtcaaccc acgtgctcac ccaatgtagc ttgacctaga gttgtcgcca 1680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 1740

1681 ttctaggaac tctcaaaagc ggggcttctt gcaaaaggtt actactcgtg aaaatttcaa 1740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc 1800

1741 gacgatacac cgcgccataa tagggcataa ctgcggcccg ttctcgttga gccagcggcg 1800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg 1860

1801 tatgtgataa gagtcttact gaaccaactc atgagtggtc agtgtctttt cgtagaatgc 1860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg 1920

1861 ctaccgtact gtcattctct taatacgtca cgacggtatt ggtactcact attgtgacgc 1920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac 1980

1921 cggttgaatg aagactgttg ctagcctcct ggcttcctcg attggcgaaa aaacgtgttg 1980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca 2040

1981 taccccctag tacattgagc ggaactagca acccttggcc tcgacttact tcggtatggt 2040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... FspI(2089) ..........

2041 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta 2100

2041 ttgctgctcg cactgtggtg ctacggacat cgttaccgtt gttgcaacgc gtttgataat 2100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 2160

2101 tgaccgcttg atgaatgaga tcgaagggcc gttgttaatt atctgaccta cctccgccta 2160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 2220

2161 tttcaacgtc ctggtgaaga cgcgagccgg gaaggccgac cgaccaaata acgactattt 2220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 2280

2221 agacctcggc cactcgcacc cagagcgcca tagtaacgtc gtgaccccgg tctaccattc 2280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 2340

2281 gggagggcat agcatcaata gatgtgctgc ccctcagtcc gttgatacct acttgcttta 2340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 2400

2341 tctgtctagc gactctatcc acggagtgac taattcgtaa ccattgacag tctggttcaa 2400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg 2460

2401 atgagtatat atgaaatcta actaaatttt gaagtaaaaa ttaaattttc ctagatccac 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga 2520

2461 ttctaggaaa aactattaga gtactggttt tagggaattg cactcaaaag caaggtgact 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 2580

2521 cgcagtctgg ggcatctttt ctagtttcct agaagaactc taggaaaaaa agacgcgcat 2580

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 2640

2581 tagacgacga acgtttgttt ttttggtggc gatggtcgcc accaaacaaa cggcctagtt 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 2700

2641 ctcgatggtt gagaaaaagg cttccattga ccgaagtcgt ctcgcgtcta tggtttatga 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 2760

2701 caggaagatc acatcggcat caatccggtg gtgaagttct tgagacatcg tggcggatgt 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 2820

2761 atggagcgag acgattagga caatggtcac cgacgacggt caccgctatt cagcacagaa 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 2880

2821 tggcccaacc tgagttctgc tatcaatggc ctattccgcg tcgccagccc gacttgcccc 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 2940

2881 ccaagcacgt gtgtcgggtc gaacctcgct tgctggatgt ggcttgactc tatggatgtc 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 3000

2941 gcactcgata ctctttcgcg gtgcgaaggg cttccctctt tccgcctgtc cataggccat 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 3060

3001 tcgccgtccc agccttgtcc tctcgcgtgc tccctcgaag gtcccccttt gcggaccata 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 3120

3061 gaaatatcag gacagcccaa agcggtggag actgaactcg cagctaaaaa cactacgagc 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 3180

3121 agtccccccg cctcggatac ctttttgcgg tcgttgcgcc ggaaaaatgc caaggaccgg 3180

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 3240

3181 aaaacgaccg gaaaacgagt gtacaagaaa ggacgcaata ggggactaag acacctattg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 3300

3241 gcataatggc ggaaactcac tcgactatgg cgagcggcgt cggcttgctg gctcgcgtcg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gagtcagtga gcgaggaagc ggaag..... .......... .......... .......... 3325

3301 ctcagtcact cgctccttcg ccttc..... .......... .......... .......... 3325

.......... .......... .......... .......... .......... ..........