Sequence of pAsRed2-N1

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Sequence of pAsRed2-N1

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Sequence of pAsRed2-N1 : 4712 BP; 1104 A; 1301 C; 1244 G; 1063 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ....... CMV_immearly_pro .. CMV_fwd_pri CMV_pro .....

.......... .......... .......... .......... .......... ..........

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

BglII,XhoI SacI,HindIII EcoRI PstI(638),SalI(639)

601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 660

601 ggcctgagtc tagagctcga gttcgaagct taagacgtca gctgccatgg cgcccgggcc 660

.......... .......... .......... .......... .......... ..........

AgeI(666) .......... .......... .......... .......... ..........

661 gatccaccgg tcgccaccat ggcctctttg ctgaagaaga ccatgccctt caggaccacc 720

661 ctaggtggcc agcggtggta ccggagaaac gacttcttct ggtacgggaa gtcctggtgg 720

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

721 atcgagggca ccgtgaacgg ccactacttc aagtgcaccg gcaagggcga gggcaacccc 780

721 tagctcccgt ggcacttgcc ggtgatgaag ttcacgtggc cgttcccgct cccgttgggg 780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ctggagggca cccaggagat gaagatcgag gtgatcgagg gcggccccct gcccttcgcc 840

781 gacctcccgt gggtcctcta cttctagctc cactagctcc cgccggggga cgggaagcgg 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttccacatcc tgtccacctc ctgcatgtac ggctccaagg ccttcatcaa gtacgtgtcc 900

841 aaggtgtagg acaggtggag gacgtacatg ccgaggttcc ggaagtagtt catgcacagg 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggcatccccg actacttcaa gcagtccctc cccgagggct tcacctggga gcgcaccacc 960

901 ccgtaggggc tgatgaagtt cgtcagggag gggctcccga agtggaccct cgcgtggtgg 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 acctacgagg acggcggctt cctgaccgcc caccaggaca cctccctgga cggcgactgc 1020

961 tggatgctcc tgccgccgaa ggactggcgg gtggtcctgt ggagggacct gccgctgacg 1020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctggtgtaca aggtgaagat cctgggcaac aacttccccg ccgacggccc cgtgatgcag 1080

1021 gaccacatgt tccacttcta ggacccgttg ttgaaggggc ggctgccggg gcactacgtc 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aacaaggccg gccgctggga gccctccacc gagatcgtgt acgaggtgga cggcgtgctg 1140

1081 ttgttccggc cggcgaccct cgggaggtgg ctctagcaca tgctccacct gccgcacgac 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgcggccagt ccctgatggc cctggagtgc cccggcggtc gccacctgac ctgccacctg 1200

1141 gcgccggtca gggactaccg ggacctcacg gggccgccag cggtggactg gacggtggac 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cacaccacct accgctccaa gaagcccgcc tccgccctga agatgcccgg cttccacttc 1260

1201 gtgtggtgga tggcgaggtt cttcgggcgg aggcgggact tctacgggcc gaaggtgaag 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaggaccacc gcatcgagat cctggaggag gtggagaagg gcaagtgcta caagcagtac 1320

1261 ctcctggtgg cgtagctcta ggacctcctc cacctcttcc cgttcacgat gttcgtcatg 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gaggccgccg tgggccgcta ctgcgacgcc gccccctcca agctgggcca caactgaagc 1380

1321 ctccggcggc acccggcgat gacgctgcgg cgggggaggt tcgacccggt gttgacttcg 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa 1440

1381 ccggcgctga gatctagtat tagtcggtat ggtgtaaaca tctccaaaat gaacgaaatt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... HpaI(1499)

1441 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta 1500

1441 ttttggaggg tgtggagggg gacttggact ttgtatttta cttacgttaa caacaacaat 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 1560

1501 tgaacaaata acgtcgaata ttaccaatgt ttatttcgtt atcgtagtgt ttaaagtgtt 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... AflII(1618)

1561 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 1620

1561 tatttcgtaa aaaaagtgac gtaagatcaa caccaaacag gtttgagtag ttacatagaa 1620

.......... .......... ......... EBV_rev_pri ......... ..........

.......... .......... .......... .......... .......... ..........

1621 aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc 1680

1621 ttccgcattt aacattcgca attataaaac aattttaagc gcaatttaaa aacaatttag 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag 1740

1681 tcgagtaaaa aattggttat ccggctttag ccgttttagg gaatatttag ttttcttatc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg 1800

1741 tggctctatc ccaactcaca acaaggtcaa accttgttct caggtgataa tttcttgcac 1800

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca 1860

1801 ctgaggttgc agtttcccgc tttttggcag atagtcccgc taccgggtga tgcacttggt 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa 1920

1861 agtgggatta gttcaaaaaa ccccagctcc acggcatttc gtgatttagc cttgggattt 1920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg 1980

1921 ccctcggggg ctaaatctcg aactgcccct ttcggccgct tgcaccgctc tttccttccc 1980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta 2040

1981 ttctttcgct ttcctcgccc gcgatcccgc gaccgttcac atcgccagtg cgacgcgcat 2040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg 2100

2041 tggtggtgtg ggcggcgcga attacgcggc gatgtcccgc gcagtccacc gtgaaaagcc 2100

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 2160

2101 cctttacacg cgccttgggg ataaacaaat aaaaagattt atgtaagttt atacataggc 2160

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

2161 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag 2220

2161 gagtactctg ttattgggac tatttacgaa gttattataa ctttttcctt ctcaggactc 2220

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 2280

2221 cgcctttctt ggtcgacacc ttacacacag tcaatcccac acctttcagg ggtccgaggg 2280

.......... ......... pBABE_3_pri ..... SV40_enh SV40_pro ......

.......... .......... .......... .......... .......... ..........

2281 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 2340

2281 gtcgtccgtc ttcatacgtt tcgtacgtag agttaatcag tcgttggtcc acacctttca 2340

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 2400

2341 ggggtccgag gggtcgtccg tcttcatacg tttcgtacgt agagttaatc agtcgttggt 2400

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 2460

2401 atcagggcgg ggattgaggc gggtagggcg gggattgagg cgggtcaagg cgggtaagag 2460

.......... ...... SV40_enh SV40_pro ..... SV40_origin .........

.......... .......... .......... .......... .......... ..........

2461 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 2520

2461 gcggggtacc gactgattaa aaaaaataaa tacgtctccg gctccggcgg agccggagac 2520

.......... ... SV40_pro SV40_origin SV40pro_F_pri ... ..........

.......... .......... .......... .......... .......... ClaI(2576)

2521 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat 2580

2521 tcgataaggt cttcatcact cctccgaaaa aacctccgga tccgaaaacg tttctagcta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct 2640

2581 gttctctgtc ctactcctag caaagcgtac taacttgttc tacctaacgt gcgtccaaga 2640

.......... .......... .......... ........ ORF 1 Neomycin ........

.......... .......... .......... .......... .......... ..........

2641 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc 2700

2641 ggccggcgaa cccacctctc cgataagccg atactgaccc gtgttgtctg ttagccgacg 2700

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc 2760

2701 agactacggc ggcacaaggc cgacagtcgc gtccccgcgg gccaagaaaa acagttctgg 2760

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc 2820

2761 ctggacaggc cacgggactt acttgacgtt ctgctccgtc gcgccgatag caccgaccgg 2820

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... FspI(2838) .......... .......... .......... ..........

2821 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg 2880

2821 tgctgcccgc aaggaacgcg tcgacacgag ctgcaacagt gacttcgccc ttccctgacc 2880

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag 2940

2881 gacgataacc cgcttcacgg ccccgtccta gaggacagta gagtggaacg aggacggctc 2940

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc 3000

2941 tttcataggt agtaccgact acgttacgcc gccgacgtat gcgaactagg ccgatggacg 3000

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt 3060

3001 ggtaagctgg tggttcgctt tgtagcgtag ctcgctcgtg catgagccta ccttcggcca 3060

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc 3120

3061 gaacagctag tcctactaga cctgcttctc gtagtccccg agcgcggtcg gcttgacaag 3120

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc 3180

3121 cggtccgagt tccgctcgta cgggctgccg ctcctagagc agcactgggt accgctacgg 3180

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg 3240

3181 acgaacggct tatagtacca ccttttaccg gcgaaaagac ctaagtagct gacaccggcc 3240

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag 3300

3241 gacccacacc gcctggcgat agtcctgtat cgcaaccgat gggcactata acgacttctc 3300

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg 3360

3301 gaaccgccgc ttacccgact ggcgaaggag cacgaaatgc catagcggcg agggctaagc 3360

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg 3420

3361 gtcgcgtagc ggaagatagc ggaagaactg ctcaagaaga ctcgccctga gaccccaagc 3420

.......... Neomycin ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct 3480

3421 tttactggct ggttcgctgc gggttggacg gtagtgctct aaagctaagg tggcggcgga 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc 3540

3481 agatactttc caacccgaag ccttagcaaa aggccctgcg gccgacctac taggaggtcg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga 3600

3541 cgcccctaga gtacgacctc aagaagcggg tgggatcccc ctccgattga ctttgtgcct 3600

.......... .......... .......... .......... TK_PA_term ..........

.......... .......... .......... .......... .......... ..........

3601 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc 3660

3601 tcctctgtta tggccttcct tgggcgcgat actgccgtta tttttctgtc ttattttgcg 3660

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg 3720

3661 tgccacaacc cagcaaacaa gtatttgcgc cccaagccag ggtcccgacc gtgagacagc 3720

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc 3780

3721 tatggggtgg ctctggggta accccggtta tgcgggcgca aagaaggaaa aggggtgggg 3780

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg 3840

3781 tggggggttc aagcccactt ccgggtcccg agcgtcggtt gcagccccgc cgtccgggac 3840

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta 3900

3841 ggtatcggag tccaatgagt atatatgaaa tctaactaaa ttttgaagta aaaattaaat 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 3960

3901 tttcctagat ccacttctag gaaaaactat tagagtactg gttttaggga attgcactca 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 4020

3961 aaagcaaggt gactcgcagt ctggggcatc ttttctagtt tcctagaaga actctaggaa 4020

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4021 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 4080

4021 aaaaagacgc gcattagacg acgaacgttt gtttttttgg tggcgatggt cgccaccaaa 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 4140

4081 caaacggcct agttctcgat ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 4200

4141 tctatggttt atgacaggaa gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 4260

4201 atcgtggcgg atgtatggag cgagacgatt aggacaatgg tcaccgacga cggtcaccgc 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 4320

4261 tattcagcac agaatggccc aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 4380

4321 gcccgacttg ccccccaagc acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 4440

4381 actctatgga tgtcgcactc gatactcttt cgcggtgcga agggcttccc tctttccgcc 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 4500

4441 tgtccatagg ccattcgccg tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 4560

4501 ctttgcggac catagaaata tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 4620

4561 aaaacactac gagcagtccc cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 4680

4621 atgccaagga ccggaaaacg accggaaaac gagtgtacaa gaaaggacgc aataggggac 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 attctgtgga taaccgtatt accgccatgc at........ .......... .......... 4712

4681 taagacacct attggcataa tggcggtacg ta........ .......... .......... 4712

.......... .......... .......... .......... .......... ..........