ppLOCUS pAsRed2 3334 bp DNA SYN 13-Sep-2007 DEFINITION pAsRed2 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3334 /organism="pAsRed2" /mol_type="other DNA" promoter 143..172 /label="lac_promoter" misc_feature 186..208 /label="M13_pUC_rev_primer" misc_feature 207..225 /label="M13_reverse_primer" CDS 217..987 /label="ORF frame 1" /translation="MTMITPSLHACRSTLEDPRVPVATMASLLKKTMPFRTTIEGTVN GHYFKCTGKGEGNPLEGTQEMKIEVIEGGPLPFAFHILSTSCMYGSKAFIKYVSGIPD YFKQSLPEGFTWERTTTYEDGGFLTAHQDTSLDGDCLVYKVKILGNNFPADGPVMQNK AGRWEPSTEIVYEVDGVLRGQSLMALECPGGRHLTCHLHTTYRSKKPASALKMPGFHF EDHRIEILEEVEKGKCYKQYEAAVGRYCDAAPSKLGHN*" misc_feature complement(1124..1146) /label="pGEX_3_primer" promoter 1464..1492 /label="AmpR_promoter" CDS 1534..2394 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 1534..2394 /label="Ampicillin" /gene="Ampicillin" rep_origin 2549..3168 /label="pBR322_origin" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg 241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggcctctttg 301 ctgaagaaga ccatgccctt caggaccacc atcgagggca ccgtgaacgg ccactacttc 361 aagtgcaccg gcaagggcga gggcaacccc ctggagggca cccaggagat gaagatcgag 421 gtgatcgagg gcggccccct gcccttcgcc ttccacatcc tgtccacctc ctgcatgtac 481 ggctccaagg ccttcatcaa gtacgtgtcc ggcatccccg actacttcaa gcagtccctc 541 cccgagggct tcacctggga gcgcaccacc acctacgagg acggcggctt cctgaccgcc 601 caccaggaca cctccctgga cggcgactgc ctggtgtaca aggtgaagat cctgggcaac 661 aacttccccg ccgacggccc cgtgatgcag aacaaggccg gccgctggga gccctccacc 721 gagatcgtgt acgaggtgga cggcgtgctg cgcggccagt ccctgatggc cctggagtgc 781 cccggcggtc gccacctgac ctgccacctg cacaccacct accgctccaa gaagcccgcc 841 tccgccctga agatgcccgg cttccacttc gaggaccacc gcatcgagat cctggaggag 901 gtggagaagg gcaagtgcta caagcagtac gaggccgccg tgggccgcta ctgcgacgcc 961 gccccctcca agctgggcca caactgaagc ggccgcgact ctagaattcc aactgagcgc 1021 cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggcctact agtcggccgt 1081 acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 1141 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 1201 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 1261 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 1321 accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 1381 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 1441 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 1501 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 1561 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 1621 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 1681 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 1741 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 1801 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 1861 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 1921 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 1981 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 2041 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 2101 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 2161 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 2221 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 2281 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 2341 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 2401 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 2461 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 2521 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 2581 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 2641 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 2701 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 2761 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 2821 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 2881 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2941 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 3001 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 3061 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 3121 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 3181 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 3241 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 3301 gagcgcagcg agtcagtgag cgaggaagcg gaag // pAsRed2 "Times New RomanpC lac_pro"Times New RomanpC M13_pUC_rev_pri"Times New RomanpCɆ M13_reverse_pri"Times New Romanp ORF 1"Times New RomanpC dz pGEX_3_pri"Times New RomanpC AmpR_pro"Times New RomanpCɆ Z ORF 1"Times New Roman(p Z  Ampicillin"Times New RomanpC `  pBR322_origin"Times New Roman pPvuII"Times New Roman7 }n} pSHindIII(234),PstI(250),SalI(252),BamHI(264),SmaI(271),AgeI(276),KpnI(277),NcoI(287)"Times New Roman !);;  p+NotI(990),EcoRI(1004),SpeI(1068),ApaI(1087)"Times New Roman   p"NdeI(1279),AflII(1336),AatII(1402)"Times New Roman V7dFqUqU pFspI"Times New Roman2 #1>>M ptest"Times New Roman d-