Sequence of pAsRed2

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Sequence of pAsRed2

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Sequence of pAsRed2 : 3334 BP; 793 A; 902 C; 886 G; 753 T; 0 other;

.......... .......... .......... .......... .......... PvuII(55)

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

1 tcgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

61 tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cactcaatcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

121 agtgagtaat ccgtggggtc cgaaatgtga aatacgaagg ccgagcatac aacacacctt 180

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gc caagcttg 240

181 aacactcgcc tattgttaaa gtgtgtcctt tgtcgatact ggtactaatg cggttcgaac 240

....... M13_pUC_rev_pri .... M13_reverse_pri ORF 1 .......

PstI,SalI BamHI SmaI,AgeI276),KpnI(277) NcoI(287)

241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tcgccaccat ggcctctttg 300

241 gtacggacgt ccagctgaga tctcctaggg gcccatggcc agcggtggta ccggagaaac 300

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ctgaagaaga ccatgccctt caggaccacc atcgagggca ccgtgaacgg ccactacttc 360

301 gacttcttct ggtacgggaa gtcctggtgg tagctcccgt ggcacttgcc ggtgatgaag 360

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aagtgcaccg gcaagggcga gggcaacccc ctggagggca cccaggagat gaagatcgag 420

361 ttcacgtggc cgttcccgct cccgttgggg gacctcccgt gggtcctcta cttctagctc 420

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gtgatcgagg gcggccccct gcccttcgcc ttccacatcc tgtccacctc ctgcatgtac 480

421 cactagctcc cgccggggga cgggaagcgg aaggtgtagg acaggtggag gacgtacatg 480

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggctccaagg ccttcatcaa gtacgtgtcc ggcatccccg actacttcaa gcagtccctc 540

481 ccgaggttcc ggaagtagtt catgcacagg ccgtaggggc tgatgaagtt cgtcagggag 540

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cccgagggct tcacctggga gcgcaccacc acctacgagg acggcggctt cctgaccgcc 600

541 gggctcccga agtggaccct cgcgtggtgg tggatgctcc tgccgccgaa ggactggcgg 600

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caccaggaca cctccctgga cggcgactgc ctggtgtaca aggtgaagat cctgggcaac 660

601 gtggtcctgt ggagggacct gccgctgacg gaccacatgt tccacttcta ggacccgttg 660

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 aacttccccg ccgacggccc cgtgatgcag aacaaggccg gccgctggga gccctccacc 720

661 ttgaaggggc ggctgccggg gcactacgtc ttgttccggc cggcgaccct cgggaggtgg 720

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gagatcgtgt acgaggtgga cggcgtgctg cgcggccagt ccctgatggc cctggagtgc 780

721 ctctagcaca tgctccacct gccgcacgac gcgccggtca gggactaccg ggacctcacg 780

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cccggcggtc gccacctgac ctgccacctg cacaccacct accgctccaa gaagcccgcc 840

781 gggccgccag cggtggactg gacggtggac gtgtggtgga tggcgaggtt cttcgggcgg 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tccgccctga agatgcccgg cttccacttc gaggaccacc gcatcgagat cctggaggag 900

841 aggcgggact tctacgggcc gaaggtgaag ctcctggtgg cgtagctcta ggacctcctc 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtggagaagg gcaagtgcta caagcagtac gaggccgccg tgggccgcta ctgcgacgcc 960

901 cacctcttcc cgttcacgat gttcgtcatg ctccggcggc acccggcgat gacgctgcgg 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... NotI(990) .......... .......... ..........

961 gccccctcca agctgggcca caactgaagc ggccgcgact ctagaattcc aactgagcgc 1020

961 cgggggaggt tcgacccggt gttgacttcg ccggcgctga gatcttaagg ttgactcgcg 1020

.......... ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggcctact agtcggccgt 1080

1021 gccagcgatg gtaatggttg aacagaccac agtttttatt atccggatga tcagccggca 1080

.......... .......... .......... .......... .......... ..........

ApaI(1087) .......... .......... .......... .......... ..........

1081 acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc 1140

1081 tgcccgggaa agcagagcgc gcaaagccac tactgccact tttggagact gtgtacgtcg 1140

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

1141 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg 1200

1141 agggcctctg ccagtgtcga acagacattc gcctacggcc ctcgtctgtt cgggcagtcc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga 1260

1201 cgcgcagtcg cccacaaccg cccacagccc cgaccgaatt gatacgccgt agtctcgtct 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 1320

1261 aacatgactc tcacgtggta tacgccacac tttatggcgt gtctacgcat tcctctttta 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg 1380

1321 tggcgtagtc cgccggaatt cccggagcac tatgcggata aaaatatcca attacagtac 1380

.......... .......... .......... .......... .......... ..........

.......... ......... AatII(1402) ......... .......... ..........

1381 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 1440

1381 tattattacc aaagaatctg cagtccaccg tgaaaagccc ctttacacgc gccttgggga 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 1500

1441 taaacaaata aaaagattta tgtaagttta tacataggcg agtactctgt tattgggact 1500

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1501 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 1560

1501 atttacgaag ttattataac tttttccttc tcatactcat aagttgtaaa ggcacagcgg 1560

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

1561 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 1620

1561 gaataaggga aaaaacgccg taaaacggaa ggacaaaaac gagtgggtct ttgcgaccac 1620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 1680

1621 tttcattttc tacgacttct agtcaaccca cgtgctcacc caatgtagct tgacctagag 1680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact 1740

1681 ttgtcgccat tctaggaact ctcaaaagcg gggcttcttg caaaaggtta ctactcgtga 1740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc 1800

1741 aaatttcaag acgatacacc gcgccataat agggcataac tgcggcccgt tctcgttgag 1800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 1860

1801 ccagcggcgt atgtgataag agtcttactg aaccaactca tgagtggtca gtgtcttttc 1860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 1920

1861 gtagaatgcc taccgtactg tcattctctt aatacgtcac gacggtattg gtactcacta 1920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 1980

1921 ttgtgacgcc ggttgaatga agactgttgc tagcctcctg gcttcctcga ttggcgaaaa 1980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 2040

1981 aacgtgttgt accccctagt acattgagcg gaactagcaa cccttggcct cgacttactt 2040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(2098)

2041 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 2100

2041 cggtatggtt tgctgctcgc actgtggtgc tacggacatc gttaccgttg ttgcaacgcg 2100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 2160

2101 tttgataatt gaccgcttga tgaatgagat cgaagggccg ttgttaatta tctgacctac 2160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 2220

2161 ctccgcctat ttcaacgtcc tggtgaagac gcgagccggg aaggccgacc gaccaaataa 2220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 2280

2221 cgactattta gacctcggcc actcgcaccc agagcgccat agtaacgtcg tgaccccggt 2280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 2340

2281 ctaccattcg ggagggcata gcatcaatag atgtgctgcc cctcagtccg ttgataccta 2340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 2400

2341 cttgctttat ctgtctagcg actctatcca cggagtgact aattcgtaac cattgacagt 2400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 2460

2401 ctggttcaaa tgagtatata tgaaatctaa ctaaattttg aagtaaaaat taaattttcc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 2520

2461 tagatccact tctaggaaaa actattagag tactggtttt agggaattgc actcaaaagc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 2580

2521 aaggtgactc gcagtctggg gcatcttttc tagtttccta gaagaactct aggaaaaaaa 2580

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2581 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 2640

2581 gacgcgcatt agacgacgaa cgtttgtttt tttggtggcg atggtcgcca ccaaacaaac 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 2700

2641 ggcctagttc tcgatggttg agaaaaaggc ttccattgac cgaagtcgtc tcgcgtctat 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 2760

2701 ggtttatgac aggaagatca catcggcatc aatccggtgg tgaagttctt gagacatcgt 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 2820

2761 ggcggatgta tggagcgaga cgattaggac aatggtcacc gacgacggtc accgctattc 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 2880

2821 agcacagaat ggcccaacct gagttctgct atcaatggcc tattccgcgt cgccagcccg 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2940

2881 acttgccccc caagcacgtg tgtcgggtcg aacctcgctt gctggatgtg gcttgactct 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 3000

2941 atggatgtcg cactcgatac tctttcgcgg tgcgaagggc ttccctcttt ccgcctgtcc 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 3060

3001 ataggccatt cgccgtccca gccttgtcct ctcgcgtgct ccctcgaagg tccccctttg 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 3120

3061 cggaccatag aaatatcagg acagcccaaa gcggtggaga ctgaactcgc agctaaaaac 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 3180

3121 actacgagca gtccccccgc ctcggatacc tttttgcggt cgttgcgccg gaaaaatgcc 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 3240

3181 aaggaccgga aaacgaccgg aaaacgagtg tacaagaaag gacgcaatag gggactaaga 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 3300

3241 cacctattgg cataatggcg gaaactcact cgactatggc gagcggcgtc ggcttgctgg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gagcgcagcg agtcagtgag cgaggaagcg gaag...... .......... .......... 3334

3301 ctcgcgtcgc tcagtcactc gctccttcgc cttc...... .......... .......... 3334

.......... .......... .......... .......... .......... ..........