*B*B(LOCUS pBABE-MN-IRES-E-GFP 5944 bp DNA SYN 24-Aug-2007 DEFINITION pBABE-MN-IRES-E-GFP ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5944 /organism="pBABE-MN-IRES-E-GFP" /mol_type="other DNA" misc_feature 194..1480 /label="psi_plus_pack2" misc_feature 670..1164 /label="gag" CDS 670..1344 /label="ORF frame 1" /translation="MGPGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAE WPTFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPF VHPKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGAPIWPYEILYGAPPPLVNF PDPDMTRVTNSPSLQAHLQALYLVQHEVWRPLAARTKNNWTDRWYLTLTESATQCGSA DTRLRT*" misc_feature 1025..1047 /label="MSCV_primer" misc_feature 1025..1047 /label="pLXSN_5_primer" misc_feature 1063..1079 /label="pBABE_5_primer" misc_feature 1416..1432 /label="pBMN_5_primer" misc_feature 1549..2064 /label="IRES" misc_feature complement(2181..2202) /label="EGFP_N_primer" CDS 2124..2855 /label="EGFP" /gene="EGFP" /note="ORF frame 3" /translation="MATTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERT IFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMAD KQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNE KRDHMVLLEFVTAAGITHGMDELYK*" gene 2136..2852 /label="EGFP" /gene="EGFP" misc_feature 2789..2810 /label="EGFP_C_primer" misc_feature 2923..3516 /label="3MoMuLV_LTR" misc_feature 2965..3516 /label="5_LTR2" rep_origin complement(3803..4422) /label="pBR322_origin" CDS complement(4580..5440) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4580..5440) /label="Ampicillin" /gene="Ampicillin" ORIGIN 1 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct 61 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc 121 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc 181 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga 241 cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt 301 ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc 361 ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg agacgtccca 421 gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga tcgttttgga 481 ctctttggtg cacccccctt agaggaggga tatgtggttc tggtaggaga cgagaaccta 541 aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg 601 cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg 661 tctgaaaata tgggcccggg ccagactgtt accactccct taagtttgac cttaggtcac 721 tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg 781 gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc 841 tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga 901 cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc 961 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct 1021 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact 1081 ccttctctag gcgcccccat atggccatat gagatcttat atggggcacc cccgcccctt 1141 gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct ccaagctcac 1201 ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc acgtaccaag 1261 aacaactgga ccgaccggtg gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc 1321 gccgacacca gactaagaac ctagaacctc gctggaaagg accttacaca gtcctgctga 1381 ccacccccac cgccctcaaa gtagacggca tcgcagcttg gatacacgcc gcccacgtga 1441 aggctgccga ccccgggggt ggaccatcct ctagactgcc GGATCCATTT AAATTCGAAT 1501 TCCTGCAGGC CTCGAGGGCC GGCGCGCCGC GGCCGCTACG TAAATTCCGC CCCTCTCCCT 1561 CCCCCCCCCC TAACGTTACT GGCCGAAGCC GCTTGGAATA AGGCCGGTGT GCGTTTGTCT 1621 ATATGTTATT TTCCACCATA TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC 1681 CTGTCTTCTT GACGAGCATT CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC 1741 TGTTGAATGT CGTGAAGGAA GCAGTTCCTC TGGAAGCTTC TTGAAGACAA ACAACGTCTG 1801 TAGCGACCCT TTGCAGGCAG CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGCCAAA 1861 AGCCACGTGT ATAAGATACA CCTGCAAAGG CGGCACAACC CCAGTGCCAC GTTGTGAGTT 1921 GGATAGTTGT GGAAAGAGTC AAATGGCTCT CCTCAAGCGT ATTCAACAAG GGGCTGAAGG 1981 ATGCCCAGAA GGTACCCCAT TGTATGGGAT CTGATCTGGG GCCTCGGTGC ACATGCTTTA 2041 CATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT 2101 CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC GAGGAGCTGT 2161 TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTGAACGGC CACAAGTTCA 2221 GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT 2281 GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG 2341 TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA 2401 TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA 2461 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA 2521 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC 2581 ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC 2641 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA 2701 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA 2761 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG 2821 GGATCACTCA CGGCATGGAC GAGCTGTACA AGTAAAgcgg ccgccagcac agtggtcgac 2881 gatAAAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG ACCCCACCTG 2941 TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG GCATGGAAAA ATACATAACT 3001 GAGAATAGAG AAGTTCAGAT CAAGGTCAGG AACAGATGGA ACAGCTGAAT ATGGGCCAAA 3061 CAGGATATCT GTGGTAAGCA GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGAACAGC 3121 TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG 3181 AACAGATGGT CCCCAGATGC GGTCCAGCCC TCAGCAGTTT CTAGAGAACC ATCAGATGTT 3241 TCCAGGGTGC CCCAAGGACC TGAAATGACC CTGTGCCTTA TTTGAACTAA CCAATCAGTT 3301 CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC TCCCCGAGCT CAATAAAAGA GCCCACAACC 3361 CCTCACTCGG GGCGCCAGTC CTCCGATTGA CTGAGTCGCC CGGGTACCCG TGTATCCAAT 3421 AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT 3481 GAGTGATTGA CTACCCGTCA GCGGGGGTCT TTCATTCACA TGCAGCATGT ATCAAAATTA 3541 ATTTGGTTTT TTTTCTTAAG TATTTACATT AAATGGCCAT AGTTGCATTA ATGAATCGGC 3601 CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGCGCTCTTC CGCTTCCTCG CTCACTGACT 3661 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC 3721 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 3781 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG 3841 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA 3901 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC 3961 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC 4021 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 4081 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG 4141 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT 4201 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 4261 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 4321 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 4381 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 4441 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 4501 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTGCGCAA ATCAATCTAA AGTATATATG 4561 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 4621 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 4681 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 4741 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 4801 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 4861 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 4921 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 4981 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 5041 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 5101 CATCCGTAAG ATGCTTTTCT GTGACTGGTG agtactcaac caagtcattc tgagaatagt 5161 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata 5221 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 5281 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 5341 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 5401 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 5461 attgaagcat ttatcagggt tattgtctca tgacattaac ctataaaaat aggcgtatca 5521 cgaggccctt tcgtcttcaa gaacagcttt gctcttagga gtttcctaat acatcccaaa 5581 ctcaaatata taaagcattt gacttgttct atgccctagg gggcgggggg aagctaagcc 5641 agcttttttt aacatttaaa atgttaattc cattttaaat gcacagatgt ttttatttca 5701 taagggtttc aatgtgcatg aatgctgcaa tattcctgtt accaaagcta gtataaataa 5761 aaatagataa acgtggaaat tacttagagt ttctgtcatt aacgtttcct tcctcagttg 5821 acaacataaa tgcgctgctg agaagccagt ttgcatctgt caggatcaat ttcccattat 5881 gccagtcata ttaattacta gtcaattagt tgatttttat ttttgacata tacatgCtct 5941 tgaa //8pBABE-MN-IRES-E-GFP"Times New Roman*BC8psi_plus_pack2"Times New Roman*BC8gag"Times New Roman(*BCɆ8@ORF 1"Times New Roman*B8MSCV_pri"Times New Roman=*BC8 pLXSN_5_pri"Times New RomanR*BC8'7 pBABE_5_pri"Times New Roman=*BCɆ8 pBMN_5_pri"Times New Roman*B8 IRES"Times New Roman*BC8L' ORF 3"Times New Roman*BCɆ8X$ EGFP"Times New Roman*BC8 EGFP_N_pri"Times New Roman)*B8  EGFP_C_pri"Times New Roman)*BC8k  3MoMuLV_LTR"Times New Roman*BC8 5_LTR2"Times New Roman*BCɆ8F pBR322_origin"Times New Roman*B8@ORF 2"Times New Roman*BC8@ Ampicillin"Times New Roman *BAatII"Times New Roman8qWyE2c#2 *BBglII"Times New RomanX8H *BAgeI"Times New Roman8Y *BEBamHI(1481),BstBI(1495),EcoRI(1497),StuI(1509),XhoI(1511),SacII(1530)"Times New Roman8# #"iO *BNcoI"Times New RomanV8! *BSalI(2875),NheI(2953)"Times New Roman; 8( * --K *Btest"Times New Roman8@