Sequence of pBABE-MN-IRES-E-GFP

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Sequence of pBABE-MN-IRES-E-GFP

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Sequence of pBABE-MN-IRES-E-GFP: 5944 BP; 1413 A; 1659 C; 1458 G; 1414 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct 60

1 cgaagacgag gggctcgagt tattttctcg ggtgttgggg agtgagcccc gcggtcagga 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc 120

61 ggctaactga ctcagcgggc ccatgggcac ataggttatt tgggagaacg tcaacgtagg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc 180

121 ctgaacacca gagcgacaag gaaccctccc agaggagact cactaactga tgggcagtcg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga 240

181 cccccagaaa gtaaaccccc gagcaggccc tagccctctg gggacgggtc cctggtggct 240

.......... .......... ........ psi_plus_pack2 ........ ..........

.......... .......... .......... .......... .......... ..........

241 cccaccaccg ggaggtaagc tggccagcaa cttatctgtg tctgtccgat tgtctagtgt 300

241 gggtggtggc cctccattcg accggtcgtt gaatagacac agacaggcta acagatcaca 300

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

301 ctatgactga ttttatgcgc ctgcgtcggt actagttagc taactagctc tgtatctggc 360

301 gatactgact aaaatacgcg gacgcagcca tgatcaatcg attgatcgag acatagaccg 360

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... AatII(416)

361 ggacccgtgg tggaactgac gagttcggaa cacccggccg caaccctggg agacgtccca 420

361 cctgggcacc accttgactg ctcaagcctt gtgggccggc gttgggaccc tctgcagggt 420

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 gggacttcgg gggccgtttt tgtggcccga cctgagtcca aaaatcccga tcgttttgga 480

421 ccctgaagcc cccggcaaaa acaccgggct ggactcaggt ttttagggct agcaaaacct 480

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 ctctttggtg cacccccctt agaggaggga tatgtggttc tggtaggaga cgagaaccta 540

481 gagaaaccac gtggggggaa tctcctccct atacaccaag accatcctct gctcttggat 540

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaacagttcc cgcctccgtc tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg 600

541 tttgtcaagg gcggaggcag acttaaaaac gaaagccaaa ccctggcttc ggcgcggcgc 600

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 cgtcttgtct gctgcagcat cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg 660

601 gcagaacaga cgacgtcgta gcaagacaca acagagacag actgacacaa agacataaac 660

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........
< td>taagtttgac
661 tctgaaaata tgggcccggg ccagactgtt accactccct cttaggtcac 720

661 agacttttat acccgggccc ggtctgacaa tggtgaggga attcaaactg gaatccagtg 720

.......... ........ psi_plus_pack2 gag ORF 1 ........ ..........

.......... .......... .......... .......... .......... ..........
< td>atgtcaagaa
721 tggaaagatg tcgagcggat cgctcacaac cagtcggtag gagacgttgg 780

721 acctttctac agctcgccta gcgagtgttg gtcagccatc tacagttctt ctctgcaacc 780

.......... ... psi_plus_pack2 gag ORF 1 ... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>gatggccgcg
781 gttaccttct gctctgcaga atggccaacc tttaacgtcg agacggcacc 840

781 caatggaaga cgagacgtct taccggttgg aaattgcagc ctaccggcgc tctgccgtgg 840

.......... ... psi_plus_pack2 gag ORF 1 ... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>tttcacctgg
841 tttaaccgag acctcatcac ccaggttaag atcaaggtct cccgcatgga 900

841 aaattggctc tggagtagtg ggtccaattc tagttccaga aaagtggacc gggcgtacct 900

.......... ... psi_plus_pack2 gag ORF 1 ... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>tggcttttga
901 cacccagacc aggtccccta catcgtgacc tgggaagcct cccccctccc 960

901 gtgggtctgg tccaggggat gtagcactgg acccttcgga accgaaaact ggggggaggg 960

.......... ... psi_plus_pack2 gag ORF 1 ... .......... ..........

.......... .......... .......... .......... .......... ..........
< td>ttcctccatc
961 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc cgccccgtct 1020

961 acccagttcg ggaaacatgt gggattcgga ggcggaggag aaggaggtag gcggggcaga 1020

.......... ... psi_plus_pack2 gag ORF 1 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact 1080

1021 gagggggaac ttggaggagc aagctggggc ggagctagga gggaaatagg tcgggagtga 1080

MSCV_pri pLXSN_5_pri psi_plus_pack2 gag ORF 1 ..... pBABE_5_pri

.......... .......... ......... BglII(1112) ......... ..........

1081 ccttctctag gcgcccccat atggccatat gagatcttat atggggcacc cccgcccctt 1140

1081 ggaagagatc cgcgggggta taccggtata ctctagaata taccccgtgg gggcggggaa 1140

.......... ... psi_plus_pack2 gag ORF 1 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtaaacttcc ctgaccctga catgacaaga gttactaaca gcccctctct ccaagctcac 1200

1141 catttgaagg gactgggact gtactgttct caatgattgt cggggagaga ggttcgagtg 1200

..... gag psi_plus_pack2 ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttacaggctc tctacttagt ccagcacgaa gtctggagac ctctggcggc acgtaccaag 1260

1201 aatgtccgag agatgaatca ggtcgtgctt cagacctctg gagaccgccg tgcatggttc 1260

.......... ..... psi_plus_pack2 ORF 1 ..... .......... ..........

.......... AgeI(1274) .......... .......... .......... ..........

1261 aacaactgga ccgaccggtg gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc 1320

1261 ttgttgacct ggctggccac catggagtgg gaatggctca gccgctgtgt cacacccagg 1320

.......... ..... psi_plus_pack2 ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gccgacacca gactaagaac ctagaacctc gctggaaagg accttacaca gtcctgctga 1380

1321 cggctgtggt ctgattcttg gatcttggag cgacctttcc tggaatgtgt caggacgact 1380

........ ORF 1 psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccacccccac cgccctcaaa gtagacggca tcgcagcttg gatacacgcc g cccacgtga 1440

1381 ggtgggggtg gcgggagttt catctgccgt agcgtcgaac ctatgtgcgg cgggtgcact 1440

.......... ........ psi_plus_pack2 ........ pBMN_5_pri ..........

.......... .......... .......... ......... BamHI(1481) .........

1441 aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatccattt aaattcgaat 1500

1441 tccgacggct ggggccccca cctggtagga gatctgacgg cctaggtaaa tttaagctta 1500

........ psi_plus_pack2 ........ .......... .......... ..........

... StuI(1509),XhoI(1511) SacII(1530) ... .......... ..........

1501 tcctgcaggc ctcgagggcc ggcgcgccgc ggccgctacg taaattccgc ccctctccct 1560

1501 aggacgtccg gagctcccgg ccgcgcggcg ccggcgatgc atttaaggcg gggagaggga 1560

.......... .......... .......... .......... .......... IRES

.......... .......... .......... .......... .......... ..........

1561 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct 1620

1561 gggggggggg attgcaatga ccggcttcgg cgaaccttat tccggccaca cgcaaacaga 1620

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc 1680

1621 tatacaataa aaggtggtat aacggcagaa aaccgttaca ctcccgggcc tttggaccgg 1680

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc 1740

1681 gacagaagaa ctgctcgtaa ggatccccag aaaggggaga gcggtttcct tacgttccag 1740

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 1800

1741 acaacttaca gcacttcctt cgtcaaggag accttcgaag aacttctgtt tgttgcagac 1800

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa 1860

1801 atcgctggga aacgtccgtc gccttggggg gtggaccgct gtccacggag acgccggttt 1860

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt 1920

1861 tcggtgcaca tattctatgt ggacgtttcc gccgtgttgg ggtcacggtg caacactcaa 1920

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg 1980

1921 cctatcaaca cctttctcag tttaccgaga ggagttcgca taagttgttc cccgacttcc 1980

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta 2040

1981 tacgggtctt ccatggggta acatacccta gactagaccc cggagccacg tgtacgaaat 2040

.......... .......... IRES .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt 2100

2041 gtacacaaat cagctccaat tttttgcaga tccggggggc ttggtgcccc tgcaccaaaa 2100

.......... IRES .......... .......... .......... ..........

.......... .......... .......... NcoI(2134) .......... ..........

2101 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt 2160

2101 ggaaactttt tgtgctacta ttataccggt gttggtacca ctcgttcccg ctcctcgaca 2160

.......... .......... .......... .......... ORF 3 EGFP ..........

.......... .......... .......... .......... .......... ..........

2161 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtgaacggc cacaagttca 2220

2161 agtggcccca ccacgggtag gaccagctcg acctgccgct gcacttgccg gtgttcaagt 2220

.......... .......... ORF 3 EGFP EGFP_N_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct 2280

2221 cgcacaggcc gctcccgctc ccgctacggt ggatgccgtt cgactgggac ttcaagtaga 2280

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg 2340

2281 cgtggtggcc gttcgacggg cacgggaccg ggtgggagca ctggtgggac tggatgccgc 2340

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca 2400

2341 acgtcacgaa gtcggcgatg gggctggtgt acttcgtcgt gctgaagaag ttcaggcggt 2400

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga 2460

2401 acgggcttcc gatgcaggtc ctcgcgtggt agaagaagtt cctgctgccg ttgatgttct 2460

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca 2520

2461 gggcgcggct ccacttcaag ctcccgctgt gggaccactt ggcgtagctc gacttcccgt 2520

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc 2580

2521 agctgaagtt cctcctgccg ttgtaggacc ccgtgttcga cctcatgttg atgttgtcgg 2580

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc 2640

2581 tgttgcagat atagtaccgg ctgttcgtct tcttgccgta gttccacttg aagttctagg 2640

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca 2700

2641 cggtgttgta gctcctgccg tcgcacgtcg agcggctggt gatggtcgtc ttgtgggggt 2700

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga 2760

2701 agccgctgcc ggggcacgac gacgggctgt tggtgatgga ctcgtgggtc aggcgggact 2760

.......... .......... ORF 3 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg 2820

2761 cgtttctggg gttgctcttc gcgctagtgt accaggacga cctcaagcac tggcggcggc 2820

.......... .......... ORF 3 EGFP EGFP_C_pri .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggatcactca cggcatggac gagctgtaca agtaaagcgg ccgccagcac agtggtcgac 2880

2821 cctagtgagt gccgtacctg ctcgacatgt tcatttcgcc ggcggtcgtg tcaccagctg 2880

.......... ORF 3 EGFP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg 2940

2881 ctattttatt ttctaaaata aatcagaggt ctttttcccc ccttactttc tggggtggac 2940

.......... .......... .......... .......... ......... 3MoMuLV_LTR

.......... NheI(2953) .......... .......... .......... ..........

2941 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact 3000

2941 atccaaaccg ttcgatcgaa ttcattgcgg taaaacgttc cgtacctttt tatgtattga 3000

.......... ......... 3MoMuLV_LTR ......... 5_LTR2 ..........

.......... .......... .......... .......... .......... ..........

3001 gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa 3060

3001 ctcttatctc ttcaagtcta gttccagtcc ttgtctacct tgtcgactta tacccggttt 3060

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc 3120

3061 gtcctataga caccattcgt caaggacggg gccgagtccc ggttcttgtc taccttgtcg 3120

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag 3180

3121 acttataccc ggtttgtcct atagacacca ttcgtcaagg acggggccga gtcccggttc 3180

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt 3240

3181 ttgtctacca ggggtctacg ccaggtcggg agtcgtcaaa gatctcttgg tagtctacaa 3240

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt 3300

3241 aggtcccacg gggttcctgg actttactgg gacacggaat aaacttgatt ggttagtcaa 3300

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc 3360

3301 gcgaagagcg aagacaagcg cgcgaagacg aggggctcga gttattttct cgggtgttgg 3360

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat 3420

3361 ggagtgagcc ccgcggtcag gaggctaact gactcagcgg gcccatgggc acataggtta 3420

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct 3480

3421 tttgggagaa cgtcaacgta ggctgaacac cagagcgaca aggaaccctc ccagaggaga 3480

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gagtgattga ctacccgtca gcgggggtct ttcattcaca tgcagcatgt atcaaaatta 3540

3481 ctcactaact gatgggcagt cgcccccaga aagtaagtgt acgtcgtaca tagttttaat 3540

...... 3MoMuLV_LTR 5_LTR2 ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 atttggtttt ttttcttaag tatttacatt aaatggccat agttgcatta atgaatcggc 3600

3541 taaaccaaaa aaaagaattc ataaatgtaa tttaccggta tcaacgtaat tacttagccg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 caacgcgcgg ggagaggcgg tttgcgtatt ggcgctcttc cgcttcctcg ctcactgact 3660

3601 gttgcgcgcc cctctccgcc aaacgcataa ccgcgagaag gcgaaggagc gagtgactga 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 3720

3661 gcgacgcgag ccagcaagcc gacgccgctc gccatagtcg agtgagtttc cgccattatg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 3780

3721 ccaataggtg tcttagtccc ctattgcgtc ctttcttgta cactcgtttt ccggtcgttt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 3840

3781 tccggtcctt ggcatttttc cggcgcaacg accgcaaaaa ggtatccgag gcggggggac 3840

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3841 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 3900

3841 tgctcgtagt gtttttagct gcgagttcag tctccaccgc tttgggctgt cctgatattt 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 3960

3901 ctatggtccg caaaggggga ccttcgaggg agcacgcgag aggacaaggc tgggacggcg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 4020

3961 aatggcctat ggacaggcgg aaagagggaa gcccttcgca ccgcgaaaga gtatcgagtg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 4080

4021 cgacatccat agagtcaagc cacatccagc aagcgaggtt cgacccgaca cacgtgcttg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 4140

4081 gggggcaagt cgggctggcg acgcggaata ggccattgat agcagaactc aggttgggcc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 4200

4141 attctgtgct gaatagcggt gaccgtcgtc ggtgaccatt gtcctaatcg tctcgctcca 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 4260

4201 tacatccgcc acgatgtctc aagaacttca ccaccggatt gatgccgatg tgatcttcct 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 4320

4261 gtcataaacc atagacgcga gacgacttcg gtcaatggaa gcctttttct caaccatcga 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 4380

4321 gaactaggcc gtttgtttgg tggcgaccat cgccaccaaa aaaacaaacg ttcgtcgtct 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 4440

4381 aatgcgcgtc tttttttcct agagttcttc taggaaacta gaaaagatgc cccagactgc 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 4500

4441 gagtcacctt gcttttgagt gcaattccct aaaaccagta ctctaatagt ttttcctaga 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tcacctagat ccttttaaat taaaaatgaa gtttgcgcaa atcaatctaa agtatatatg 4560

4501 agtggatcta ggaaaattta atttttactt caaacgcgtt tagttagatt tcatatatac 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 4620

4561 tcatttgaac cagactgtca atggttacga attagtcact ccgtggatag agtcgctaga 4620

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4621 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 4680

4621 cagataaagc aagtaggtat caacggactg aggggcagca catctattga tgctatgccc 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 4740

4681 tcccgaatgg tagaccgggg tcacgacgtt actatggcgc tctgggtgcg agtggccgag 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 4800

4741 gtctaaatag tcgttatttg gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 4860

4801 gaaataggcg gaggtaggtc agataattaa caacggccct tcgatctcat tcatcaagcg 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 4920

4861 gtcaattatc aaacgcgttg caacaacggt aacgatgtcc gtagcaccac agtgcgagca 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 4980

4921 gcaaaccata ccgaagtaag tcgaggccaa gggttgctag ttccgctcaa tgtactaggg 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 5040

4981 ggtacaacac gttttttcgc caatcgagga agccaggagg ctagcaacag tcttcattca 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 5100

5041 accggcgtca caatagtgag taccaatacc gtcgtgacgt attaagagaa tgacagtacg 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 5160

5101 gtaggcattc tacgaaaaga cactgaccac tcatgagttg gttcagtaag actcttatca 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata 5220

5161 catacgccgc tggctcaacg agaacgggcc gcagttgtgc cctattatgg cgcggtgtat 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 5280

5221 cgtcttgaaa ttttcacgag tagtaacctt ttgcaagaag ccccgctttt gagagttcct 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 5340

5281 agaatggcga caactctagg tcaagctaca ttgggtgagc acgtgggttg actagaagtc 5340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 5400

5341 gtagaaaatg aaagtggtcg caaagaccca ctcgtttttg tccttccgtt ttacggcgtt 5400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 5460

5401 ttttccctta ttcccgctgt gcctttacaa cttatgagta tgagaaggaa aaagttataa 5460

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 attgaagcat ttatcagggt tattgtctca tgacattaac ctataaaaat aggcgtatca 5520

5461 taacttcgta aatagtccca ataacagagt actgtaattg gatattttta tccgcatagt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cgaggccctt tcgtcttcaa gaacagcttt gctcttagga gtttcctaat acatcccaaa 5580

5521 gctccgggaa agcagaagtt cttgtcgaaa cgagaatcct caaaggatta tgtagggttt 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ctcaaatata taaagcattt gacttgttct atgccctagg gggcgggggg aagctaagcc 5640

5581 gagtttatat atttcgtaaa ctgaacaaga tacgggatcc cccgcccccc ttcgattcgg 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 agcttttttt aacatttaaa atgttaattc cattttaaat gcacagatgt ttttatttca 5700

5641 tcgaaaaaaa ttgtaaattt tacaattaag gtaaaattta cgtgtctaca aaaataaagt 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 taagggtttc aatgtgcatg aatgctgcaa tattcctgtt accaaagcta gtataaataa 5760

5701 attcccaaag ttacacgtac ttacgacgtt ataaggacaa tggtttcgat catatttatt 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 aaatagataa acgtggaaat tacttagagt ttctgtcatt aacgtttcct tcctcagttg 5820

5761 tttatctatt tgcaccttta atgaatctca aagacagtaa ttgcaaagga aggagtcaac 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 acaacataaa tgcgctgctg agaagccagt ttgcatctgt caggatcaat ttcccattat 5880

5821 tgttgtattt acgcgacgac tcttcggtca aacgtagaca gtcctagtta aagggtaata 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gccagtcata ttaattacta gtcaattagt tgatttttat ttttgacata tacatgctct 5940

5881 cggtcagtat aattaatgat cagttaatca actaaaaata aaaactgtat atgtacgaga 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tgaa...... .......... .......... .......... .......... .......... 5944

5941 actt...... .......... .......... .......... .......... .......... 5944

.......... .......... .......... .......... .......... ..........