Sequence of pBABE-hygro

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Sequence of pBABE-hygro

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Sequence of pBABE-hygro : 5558 BP; 1265 A; 1516 C; 1436 G; 1341 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 60

1 gacgtcggac ttatacccgg tttgtcctat agacaccatt cgtcaaggac ggggccgagt 60

.......... .......... ...... 5_LTR2 3MoMuLV_LTR ...... ..........

.......... .......... .......... .......... .......... ..........

61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt < font color="#000000" style="font-size:10px" face="Courier">ggtaagcagt 120

61 cccggttctt gtctaccttg tcgacttata cccggtttgt cctatagaca ccattcgtca 120

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 180

121 aggacggggc cgagtcccgg ttcttgtcta ccaggggtct acgccaggtc gggagtcgtc 180

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 240

181 aaagatctct tggtagtcta caaaggtccc acggggttcc tggactttac tgggacacgg 240

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 300

241 aataaacttg attggttagt caagcgaaga gcgaagacaa gcgcgcgaag acgaggggct 300

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 360

301 cgagttattt tctcgggtgt tggggagtga gccccgcggt caggaggcta actgactcag 360

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 420

361 cgggcccatg ggcacatagg ttatttggga gaacgtcaac gtaggctgaa caccagagcg 420

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 480

421 acaaggaacc ctcccagagg agactcacta actgatgggc agtcgccccc agaaagtaaa 480

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 540

481 cccccgagca ggccctagcc ctctggggac gggtccctgg tggctgggtg gtggccctcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 600

541 attcgaccgg tcgttgaata gacacagaca ggctaacaga tcacagatac tgactaaaat 600

.......... .......... ........ psi_plus_pack ........ ..........

.......... SpeI(616) .......... .......... .......... ..........

601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 660

601 acgcggacgc agccatgatc aatcgattga tcgagacata gaccgcctgg gcaccacctt 660

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 720

661 gactgctcaa gacttgtggg ccggcgttgg gaccctctgc agggtccctg aaacccccgg 720

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 780

721 caaaaacacc gggctggact ccttccctca gctacacctt aggctggggc agtcctatac 780

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 840

781 accaagacca tcctctgctc ttggattttg tcaagggcgg aggcagactt aaaaacgaaa 840

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 900

841 gccaaacctt ggcttcggcg cgcagaacag acgacgtcgt agcaagacac aacagagaca 900

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 960

901 gactgacaca aagacataaa cagactttta atcccggtct gacaatggtg agggaattca 960

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 1020

961 aactggaatc tagtgacctt tctacagctc gccgagcgag tgttggtcag ccatctacag 1020

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 1080

1021 ttcttctctg caacccaatg gaagacgaga cgtcttaccg gttggaaatt gcagcctacc 1080

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 1140

1081 ggcgctctgc cgtggaaatt ggctctggag tagtgggtcc aattctagtt ccagaaaagt 1140

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 1200

1141 ggaccgggcg tacctgtggg tctggtccag gggatgtagc actggaccct tcggaaccga 1200

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt 1260

1201 aaactggggg gagggaccca gttcgggaaa catgtgggat tcggaggcgg aggagaagaa 1260

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt 1320

1261 ggtaggcgcg gcagagaggg ggaacttgga ggagaaagct ggggcggagt taggagggaa 1320

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... ......... BamHI(1355) ......... ..........

1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga 1380

1321 ataggtcggg agtgaggaag agatccgcgg ccggcctagg gtcacaccac catgcatcct 1380

pBABE_5_pri psi_plus_pack .... .......... .......... ..........

.......... SalI(1397) .......... .......... .......... ..........

1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc 1440

1381 taagcggtcg tgtcaccagc tgggacacct tacacacagt caatcccaca cctttcaggg 1440

.......... .......... ......... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 1500

1441 gtccgagggg tcgtccgtct tcatacgttt cgtacgtaga gttaatcagt cgttggtcca 1500

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 1560

1501 cacctttcag gggtccgagg ggtcgtccgt cttcatacgt ttcgtacgta gagttaatca 1560

.......... ...... SV40_enh SV40_pro ...... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 1620

1561 gtcgttggta tcagggcggg gattgaggcg ggtagggcgg ggattgaggc gggtcaaggc 1620

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... .......... .......... .......... .......... ..........

1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 1680

1621 gggtaagagg cggggtaccg actgattaaa aaaaataaat acgtctccgg ctccggcgga 1680

SV40_enh SV40_pro SV40_pro SV40_origin SV40pro_F_pri ......

.......... .......... .......... .......... .......... ..........

1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 1740

1681 gccggagact cgataaggtc ttcatcactc ctccgaaaaa acctccggat ccgaaaacgt 1740

SV40_pro .......... SV40_pro .......... .......... ..........

HindIII(1743) ...... .......... .......... .......... ..........

1741 aaaagcttac catgaccgag tacaagccca tgaaaaagcc tgaactcacc gcgacgtctg 1800

1741 ttttcgaatg gtactggctc atgttcgggt actttttcgg acttgagtgg cgctgcagac 1800

.......... .......... .......... ORF 3 .......... hygroB

.......... .......... .......... .......... .......... ..........

1801 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg 1860

1801 agctcttcaa agactagctt ttcaagctgt cgcagaggct ggactacgtc gagagcctcc 1860

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa 1920

1861 cgcttcttag agcacgaaag tcgaagctac atcctcccgc acctatacag gacgcccatt 1920

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg 1980

1921 tatcgacgcg gctaccaaag atgtttctag caatacaaat agccgtgaaa cgtagccggc 1980

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cgctcccgat tccggaagtg cttgacattg gggagttcag cgagagcctg acctattgca 2040

1981 gcgagggcta aggccttcac gaactgtaac ccctcaagtc gctctcggac tggataacgt 2040

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg 2100

2041 agagggcggc acgtgtccca cagtgcaacg ttctggacgg actttggctt gacgggcgac 2100

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga 2160

2101 aagacgtcgg ccagcgcctc cggtacctac gctagcgacg ccggctagaa tcggtctgct 2160

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... NdeI(2220)

2161 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca 2220

2161 cgcccaagcc gggtaagcct ggcgttcctt agccagttat gtgatgtacc gcactaaagt 2220

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca 2280

2221 atacgcgcta acgactaggg gtacacatag tgaccgtttg acactacctg ctgtggcagt 2280

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag 2340

2281 cacgcaggca gcgcgtccga gagctactcg actacgaaac ccggctcctg acggggcttc 2340

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca 2400

2341 aggccgtgga gcacgtgcgc ctaaagccga ggttgttaca ggactgcctg ttaccggcgt 2400

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca 2460

2401 attgtcgcca gtaactgacc tcgctccgct acaagcccct aagggttatg ctccagcggt 2460

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc 2520

2461 tgtagaagaa gacctccggc accaaccgaa catacctcgt cgtctgcgcg atgaagctcg 2520

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... ......... SacII(2550) ......... .......... ..........

2521 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc 2580

2521 cctccgtagg cctcgaacgt cctagcggcg ccgaggcccg catatacgag gcgtaaccag 2580

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg 2640

2581 aactggttga gatagtctcg aaccaactgc cgttaaagct actacgtcga acccgcgtcc 2640

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc 2700

2641 cagctacgct gcgttagcag gctaggcctc ggccctgaca gcccgcatgt gtttagcggg 2700

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc 2760

2701 cgtcttcgcg ccggcagacc tggctaccga cacatcttca tgagcggcta tcacctttgg 2760

.......... ......... ORF 3 hygroB ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gacgccccag cactcgtccg agggcaaagg aatatcgata aaataaaaga ttttatttag 2820

2761 ctgcggggtc gtgagcaggc tcccgtttcc ttatagctat tttattttct aaaataaatc 2820

.......... hygroB ORF 3 .......... .......... ..........

.......... .......... .......... .......... NheI(2869) ..........

2821 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt 2880

2821 agaggtcttt ttcccccctt actttctggg gtggacatcc aaaccgttcg atcgaattca 2880

.......... .......... ......... 3MoMuLV_LTR ......... ..........

.......... .......... .......... .......... .......... ..........

2881 aacgccattt tgcaaggcat ggaaaaatac ataactgaga atagagaagt tcagatcaag 2940

2881 ttgcggtaaa acgttccgta cctttttatg tattgactct tatctcttca agtctagttc 2940

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtcaggaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc 3000

2941 cagtccttgt ctaccttgtc gacttatacc cggtttgtcc tatagacacc attcgtcaag 3000

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctgccccggc tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc 3060

3001 gacggggccg agtcccggtt cttgtctacc ttgtcgactt atacccggtt tgtcctatag 3060

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc 3120

3061 acaccattcg tcaaggacgg ggccgagtcc cggttcttgt ctaccagggg tctacgccag 3120

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cagccctcag cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa 3180

3121 gtcgggagtc gtcaaagatc tcttggtagt ctacaaaggt cccacggggt tcctggactt 3180

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc 3240

3181 tactgggaca cggaataaac ttgattggtt agtcaagcga agagcgaaga caagcgcgcg 3240

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc 3300

3241 aagacgaggg gctcgagtta ttttctcggg tgttggggag tgagccccgc ggtcaggagg 3300

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga 3360

3301 ctaactgact cagcgggccc atgggcacat aggttatttg ggagaacgtc aacgtaggct 3360

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg 3420

3361 gaacaccaga gcgacaagga accctcccag aggagactca ctaactgatg ggcagtcgcc 3420

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gggtctttca tttccgactt gtggtctcgc tgccttggga gggtctcctc tgagtgattg 3480

3421 cccagaaagt aaaggctgaa caccagagcg acggaaccct cccagaggag actcactaac 3480

3MoMuLV_LTR 5_LTR2 .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 actacccgtc agcgggggtc ttcacatgca gcatgtatca aaattaattt ggtttttttt 3540

3481 tgatgggcag tcgcccccag aagtgtacgt cgtacatagt tttaattaaa ccaaaaaaaa 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cttaagtatt tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag 3600

3541 gaattcataa atgtaattta ccggtatcaa cgtaattact tagccggttg cgcgcccctc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aggcggtttg cgtattggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 3660

3601 tccgccaaac gcataaccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 3720

3661 caagccgacg ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 3780

3721 agtcccctat tgcgtccttt cttgtacact cgttttccgg tcgttttccg gtccttggca 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 3840

3781 tttttccggc gcaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt 3840

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 3900

3841 ttagctgcga gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 3960

3901 gggggacctt cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 4020

3961 aggcggaaag agggaagccc ttcgcaccgc gaaagagtat cgagtgcgac atccatagag 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 4080

4021 tcaagccaca tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 4140

4081 ctggcgacgc ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 4200

4141 agcggtgacc gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 4260

4201 tgtctcaaga acttcaccac cggattgatg ccgatgtgat cttcctgtca taaaccatag 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 4320

4261 acgcgagacg acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 4380

4321 gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 4440

4381 tttcctagag ttcttctagg aaactagaaa agatgcccca gactgcgagt caccttgctt 4440

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 4500

4441 ttgagtgcaa ttccctaaaa ccagtactct aatagttttt cctagaagtg gatctaggaa 4500

.......... .......... .......... .......... .......... ..........

.......... .......... NotI(4522) .......... .......... ..........

4501 ttaaattaaa aatgaagttt gcggccgcaa atcaatctaa agtatatatg agtaaacttg 4560

4501 aatttaattt ttacttcaaa cgccggcgtt tagttagatt tcatatatac tcatttgaac 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 4620

4561 cagactgtca atggttacga attagtcact ccgtggatag agtcgctaga cagataaagc 4620

.......... .......... .......... Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 4680

4621 aagtaggtat caacggactg aggggcagca catctattga tgctatgccc tcccgaatgg 4680

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 4740

4681 tagaccgggg tcacgacgtt actatggcgc tctgggtgcg agtggccgag gtctaaatag 4740

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 4800

4741 tcgttatttg gtcggtcggc cttcccggct cgcgtcttca ccaggacgtt gaaataggcg 4800

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 4860

4801 gaggtaggtc agataattaa caacggccct tcgatctcat tcatcaagcg gtcaattatc 4860

.......... .......... Ampicillin .......... .......... ..........

FspI(4865) .......... .......... .......... .......... ..........

4861 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat 4920

4861 aaacgcgttg caacaacggt aacgatgtcc gtagcaccac agtgcgagca gcaaaccata 4920

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 4980

4921 ccgaagtaag tcgaggccaa gggttgctag ttccgctcaa tgtactaggg ggtacaacac 4980

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 5040

4981 gttttttcgc caatcgagga agccaggagg ctagcaacag tcttcattca accggcgtca 5040

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 5100

5041 caatagtgag taccaatacc gtcgtgacgt attaagagaa tgacagtacg gtaggcattc 5100

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 5160

5101 tacgaaaaga cactgaccac tcatgagttg gttcagtaag actcttatca catacgccgc 5160

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt 5220

5161 tggctcaacg agaacgggcc gcagttgtgc cctattatgg cgcggtgtat cgtcttgaaa 5220

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 5280

5221 ttttcacgag tagtaacctt ttgcaagaag ccccgctttt gagagttcct agaatggcga 5280

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 5340

5281 caactctagg tcaagctaca ttgggtgagc acgtgggttg actagaagtc gtagaaaatg 5340

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 5400

5341 aaagtggtcg caaagaccca ctcgtttttg tccttccgtt ttacggcgtt ttttccctta 5400

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 5460

5401 ttcccgctgt gcctttacaa cttatgagta tgagaaggaa aaagttataa taacttcgta 5460

.......... Ampicillin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 5520

5461 aatagtccca ataacagagt actcgcctat gtataaactt acataaatct ttttatttgt 5520

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aataggggtt ccgcgcacat ttccccgaaa agtgccac.. .......... .......... 5558

5521 ttatccccaa ggcgcgtgta aaggggcttt tcacggtg.. .......... .......... 5558

.......... .......... .......... .......... .......... ..........