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pBABE-puro
Gene/insert name: none
Insert size (bp): Unknown
Vector backbone: pBABE-puro (Search Vector Database)
Type of vector: Mammalian expression,Retroviral
Backbone size (bp): 5169
5' Sequencing primer: pBABE 5' (List of Sequencing Primers)
3' Sequencing primer: pBABE 3'
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: Puromycin
Plasmid Provided In: DH5a
Principal Investigator: Bob Weinberg
Comments: Morgenstern JP, Land H., 1990, Nucleic Acids Research 18(12):3587-96.
If you are using the pBABE protocol from the Weinberg Lab to generate virus, please note that Addgene supplies pCL-Eco (#12371), VSV-G (#8454), and a gag/pol expression vector (#8455).