Sequence of pBABE-puro

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Sequence of pBABE-puro

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Sequence of pBABE-puro : 5169 BP; 1148 A; 1489 C; 1336 G; 1196 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 60

1 gacgtcggac ttatacccgg tttgtcctat agacaccatt cgtcaaggac ggggccgagt 60

.......... .......... ...... 5_LTR2 3MoMuLV_LTR ...... ..........

.......... .......... .......... .......... .......... ..........

61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt < font color="#000000" style="font-size:10px" face="Courier">ggtaagcagt 120

61 cccggttctt gtctaccttg tcgacttata cccggtttgt cctatagaca ccattcgtca 120

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 180

121 aggacggggc cgagtcccgg ttcttgtcta ccaggggtct acgccaggtc gggagtcgtc 180

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 240

181 aaagatctct tggtagtcta caaaggtccc acggggttcc tggactttac tgggacacgg 240

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 300

241 aataaacttg attggttagt caagcgaaga gcgaagacaa gcgcgcgaag acgaggggct 300

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 360

301 cgagttattt tctcgggtgt tggggagtga gccccgcggt caggaggcta actgactcag 360

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 420

361 cgggcccatg ggcacatagg ttatttggga gaacgtcaac gtaggctgaa caccagagcg 420

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 480

421 acaaggaacc ctcccagagg agactcacta actgatgggc agtcgccccc agaaagtaaa 480

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 540

481 cccccgagca ggccctagcc ctctggggac gggtccctgg tggctgggtg gtggccctcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 600

541 attcgaccgg tcgttgaata gacacagaca ggctaacaga tcacagatac tgactaaaat 600

.......... .......... ........ psi_plus_pack ........ ..........

.......... SpeI(616) .......... .......... .......... ..........

601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 660

601 acgcggacgc agccatgatc aatcgattga tcgagacata gaccgcctgg gcaccacctt 660

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 720

661 gactgctcaa gacttgtggg ccggcgttgg gaccctctgc agggtccctg aaacccccgg 720

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 780

721 caaaaacacc gggctggact ccttccctca gctacacctt aggctggggc agtcctatac 780

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 840

781 accaagacca tcctctgctc ttggattttg tcaagggcgg aggcagactt aaaaacgaaa 840

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 900

841 gccaaacctt ggcttcggcg cgcagaacag acgacgtcgt agcaagacac aacagagaca 900

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 960

901 gactgacaca aagacataaa cagactttta atcccggtct gacaatggtg agggaattca 960

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 1020

961 aactggaatc tagtgacctt tctacagctc gccgagcgag tgttggtcag ccatctacag 1020

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 1080

1021 ttcttctctg caacccaatg gaagacgaga cgtcttaccg gttggaaatt gcagcctacc 1080

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 1140

1081 ggcgctctgc cgtggaaatt ggctctggag tagtgggtcc aattctagtt ccagaaaagt 1140

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 1200

1141 ggaccgggcg tacctgtggg tctggtccag gggatgtagc actggaccct tcggaaccga 1200

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt 1260

1201 aaactggggg gagggaccca gttcgggaaa catgtgggat tcggaggcgg aggagaagaa 1260

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt 1320

1261 ggtaggcgcg gcagagaggg ggaacttgga ggagaaagct ggggcggagt taggagggaa 1320

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... ......... BamHI(1355) ......... ..........

1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga 1380

1321 ataggtcggg agtgaggaag agatccgcgg ccggcctagg gtcacaccac catgcatcct 1380

pBABE_5_pri psi_plus_pack .... .......... .......... ..........

.......... SalI(1397) .......... .......... .......... ..........

1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc 1440

1381 taagcggtcg tgtcaccagc tgggacacct tacacacagt caatcccaca cctttcaggg 1440

.......... .......... ......... pBABE_3_pri SV40_enh SV40_pro

.......... .......... .......... .......... .......... ..........

1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 1500

1441 gtccgagggg tcgtccgtct tcatacgttt cgtacgtaga gttaatcagt cgttggtcca 1500

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 1560

1501 cacctttcag gggtccgagg ggtcgtccgt cttcatacgt ttcgtacgta gagttaatca 1560

.......... ...... SV40_enh SV40_pro ...... .......... SV40_pro

.......... .......... .......... .......... .......... ..........

1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 1620

1561 gtcgttggta tcagggcggg gattgaggcg ggtagggcgg ggattgaggc gggtcaaggc 1620

.......... .. SV40_enh SV40_pro SV40_pro . SV40_origin .........

.......... NcoI(1634) .......... .......... .......... ..........

1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 1680

1621 gggtaagagg cggggtaccg actgattaaa aaaaataaat acgtctccgg ctccggcgga 1680

SV40_enh SV40_pro SV40_pro SV40_origin SV40pro_F_pri ......

.......... .......... .......... .......... .......... ..........

1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 1740

1681 gccggagact cgataaggtc ttcatcactc ctccgaaaaa acctccggat ccgaaaacgt 1740

SV40_pro .......... SV40_pro .......... .......... ..........

HindIII(1743) ...... .......... .......... .......... ..........

1741 aaaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 1800

1741 ttttcgaatg gtactggctc atgttcgggt gccacgcgga gcggtgggcg ctgctgcagg 1800

.......... .......... ...... ORF 3 puro(0-600) ...... ..........

.......... .......... .......... .......... .......... ..........

1801 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 1860

1801 ggtcccggca tgcgtgggag cggcggcgca agcggctgat ggggcggtgc gcggtgtggc 1860

.......... ...... ORF 3 puro(0-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 1920

1861 agctaggcct ggcggtgtag ctcgcccagt ggctcgacgt tcttgagaag gagtgcgcgc 1920

.......... ...... ORF 3 puro(0-600) ...... .......... ..........

.......... .......... .......... ......... SacII(1966) .........

1921 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 1980

1921 agcccgagct gtagccgttc cacacccagc gcctgctgcc gcggcgccac cgccagacct 1980

.......... ...... ORF 3 puro(0-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 2040

1981 ggtgcggcct ctcgcagctt cgcccccgcc acaagcggct ctagccgggc gcgtaccggc 2040

.......... ...... ORF 3 puro(0-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 2100

2041 tcaactcgcc aagggccgac cggcgcgtcg ttgtctacct tccggaggac cgcggcgtgg 2100

.......... ...... ORF 3 puro(0-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 2160

2101 ccgggttcct cgggcgcacc aaggaccggt ggcagccgca gagcgggctg gtggtcccgt 2160

.......... ...... ORF 3 puro(0-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 2220

2161 tcccagaccc gtcgcggcag cacgaggggc ctcacctccg ccggctcgcg cggccccacg 2220

.......... ...... ORF 3 puro(0-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 2280

2221 ggcggaagga cctctggagg cgcggggcgt tggaggggaa gatgctcgcc gagccgaagt 2280

.......... ...... ORF 3 puro(0-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg acccgcaagc 2340

2281 ggcagtggcg gctgcagctc acgggcttcc tggcgcgctg gaccacgtac tgggcgttcg 2340

.......... ...... ORF 3 puro(0-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc 2400

2341 ggccacggac tgcgggcggg gtgctgggcg tcgcgggctg gctttcctcg cgtgctgggg 2400

ORF 3 puro(0-600) .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atgcatcgat aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc 2460

2401 tacgtagcta ttttattttc taaaataaat cagaggtctt tttcccccct tactttctgg 2460

.......... .......... .......... .......... ......... 3MoMuLV_LTR

.......... NheI(2480) .......... .......... .......... ..........

2461 ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca t ggaaaaata 2520

2461 ggtggacatc caaaccgttc gatcgaattc attgcggtaa aacgttccgt acctttttat 2520

.......... ......... 3MoMuLV_LTR ......... 5_LTR2 ..........

.......... .......... .......... .......... .......... ..........

2521 cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg 2580

2521 gtattgactc ttatctcttc aagtctagtt ccagtccttg tctaccttgt cgacttatac 2580

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg 2640

2581 ccggtttgtc ctatagacac cattcgtcaa ggacggggcc gagtcccggt tcttgtctac 2640

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag 2700

2641 cttgtcgact tatacccggt ttgtcctata gacaccattc gtcaaggacg gggccgagtc 2700

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc 2760

2701 ccggttcttg tctaccaggg gtctacgcca ggtcgggagt cgtcaaagat ctcttggtag 2760

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca 2820

2761 tctacaaagg tcccacgggg ttcctggact ttactgggac acggaataaa cttgattggt 2820

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc 2880

2821 tagtcaagcg aagagcgaag acaagcgcgc gaagacgagg ggctcgagtt attttctcgg 2880

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt 2940

2881 gtgttgggga gtgagccccg cggtcaggag gctaactgac tcagcgggcc catgggcaca 2940

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt 3000

2941 taggttattt gggagaacgt caacgtaggc tgaacaccag agcgacaagg aaccctccca 3000

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttccgact tgtggtctcg 3060

3001 gaggagactc actaactgat gggcagtcgc ccccagaaag taaaggctga acaccagagc 3060

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctgccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt cttcacatgc 3120

3061 gacggaaccc tcccagagga gactcactaa ctgatgggca gtcgccccca gaagtgtacg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt 3180

3121 tcgtacatag ttttaattaa accaaaaaaa agaattcata aatgtaattt accggtatca 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc gctcttccgc 3240

3181 acgtaattac ttagccggtt gcgcgcccct ctccgccaaa cgcataaccg cgagaaggcg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 3300

3241 aaggagcgag tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 3360

3301 gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 3420

3361 tcgttttccg gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt 3420

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 3480

3421 atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 3540

3481 gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 3600

3541 acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 3660

3601 cgaaagagta tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 3720

3661 cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 3780

3721 agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 3840

3781 ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 3900

3841 gccgatgtga tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 3960

3901 tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 4020

3961 acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 4080

4021 aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NotI(4133)

4081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt tgcggccgca 4140

4081 taatagtttt tcctagaagt ggatctagga aaatttaatt tttacttcaa acgccggcgt 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 4200

4141 ttagttagat ttcatatata ctcatttgaa ccagactgtc aatggttacg aattagtcac 4200

.......... .......... .......... .......... Ampicillin ..........

.......... .......... .......... .......... .......... ..........

4201 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 4260

4201 tccgtggata gagtcgctag acagataaag caagtaggta tcaacggact gaggggcagc 4260

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 4320

4261 acatctattg atgctatgcc ctcccgaatg gtagaccggg gtcacgacgt tactatggcg 4320

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 4380

4321 ctctgggtgc gagtggccga ggtctaaata gtcgttattt ggtcggtcgg ccttcccggc 4380

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 4440

4381 tcgcgtcttc accaggacgt tgaaataggc ggaggtaggt cagataatta acaacggccc 4440

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... FspI(4476) .......... ..........

4441 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 4500

4441 ttcgatctca ttcatcaagc ggtcaattat caaacgcgtt gcaacaacgg taacgatgtc 4500

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 4560

4501 cgtagcacca cagtgcgagc agcaaaccat accgaagtaa gtcgaggcca agggttgcta 4560

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 4620

4561 gttccgctca atgtactagg gggtacaaca cgttttttcg ccaatcgagg aagccaggag 4620

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 4680

4621 gctagcaaca gtcttcattc aaccggcgtc acaatagtga gtaccaatac cgtcgtgacg 4680

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 4740

4681 tattaagaga atgacagtac ggtaggcatt ctacgaaaag acactgacca ctcatgagtt 4740

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac 4800

4741 ggttcagtaa gactcttatc acatacgccg ctggctcaac gagaacgggc cgcagttgtg 4800

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 4860

4801 ccctattatg gcgcggtgta tcgtcttgaa attttcacga gtagtaacct tttgcaagaa 4860

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 4920

4861 gccccgcttt tgagagttcc tagaatggcg acaactctag gtcaagctac attgggtgag 4920

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 4980

4921 cacgtgggtt gactagaagt cgtagaaaat gaaagtggtc gcaaagaccc actcgttttt 4980

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 5040

4981 gtccttccgt tttacggcgt tttttccctt attcccgctg tgcctttaca acttatgagt 5040

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 5100

5041 atgagaagga aaaagttata ataacttcgt aaatagtccc aataacagag tactcgccta 5100

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

5101 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 5160

5101 tgtataaact tacataaatc tttttatttg tttatcccca aggcgcgtgt aaaggggctt 5160

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aagtgccac. .......... .......... .......... .......... .......... 5169

5161 ttcacggtg. .......... .......... .......... .......... .......... 5169

.......... .......... .......... .......... .......... ..........