Sequence of pBABE

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

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Sequence of pBABE

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Sequence of pBABE-neo : 5330 BP; 1200 A; 1459 C; 1372 G; 1299 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 60

1 gacgtcggac ttatacccgg tttgtcctat agacaccatt cgtcaaggac ggggccgagt 60

.......... .......... ...... 5_LTR2 3MoMuLV_LTR ...... ..........

.......... .......... .......... .......... .......... ..........

61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt < font color="#000000" style="font-size:10px" face="Courier">ggtaagcagt 120

61 cccggttctt gtctaccttg tcgacttata cccggtttgt cctatagaca ccattcgtca 120

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 180

121 aggacggggc cgagtcccgg ttcttgtcta ccaggggtct acgccaggtc gggagtcgtc 180

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 240

181 aaagatctct tggtagtcta caaaggtccc acggggttcc tggactttac tgggacacgg 240

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 300

241 aataaacttg attggttagt caagcgaaga gcgaagacaa gcgcgcgaag acgaggggct 300

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 360

301 cgagttattt tctcgggtgt tggggagtga gccccgcggt caggaggcta actgactcag 360

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 420

361 cgggcccatg ggcacatagg ttatttggga gaacgtcaac gtaggctgaa caccagagcg 420

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 480

421 acaaggaacc ctcccagagg agactcacta actgatgggc agtcgccccc agaaagtaaa 480

.......... ...... 5_LTR2 3MoMuLV_LTR ...... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 540

481 cccccgagca ggccctagcc ctctggggac gggtccctgg tggctgggtg gtggccctcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 600

541 attcgaccgg tcgttgaata gacacagaca ggctaacaga tcacagatac tgactaaaat 600

.......... .......... ........ psi_plus_pack ........ ..........

.......... SpeI(616) .......... .......... .......... ..........

601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 660

601 acgcggacgc agccatgatc aatcgattga tcgagacata gaccgcctgg gcaccacctt 660

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... AatII(701) ..........

661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 720

661 gactgctcaa gacttgtggg ccggcgttgg gaccctctgc agggtccctg aaacccccgg 720

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 780

721 caaaaacacc gggctggact ccttccctca gctacacctt aggctggggc agtcctatac 780

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 840

781 accaagacca tcctctgctc ttggattttg tcaagggcgg aggcagactt aaaaacgaaa 840

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 900

841 gccaaacctt ggcttcggcg cgcagaacag acgacgtcgt agcaagacac aacagagaca 900

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt 960

901 gactgacaca aagacataaa cagactttta atcccggtct gacaatggtg agggaattca 960

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 ttgaccttag atcactggaa agatgtcgag cggctcgctc acaaccagtc ggtagatgtc 1020

961 aactggaatc tagtgacctt tctacagctc gccgagcgag tgttggtcag ccatctacag 1020

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg 1080

1021 ttcttctctg caacccaatg gaagacgaga cgtcttaccg gttggaaatt gcagcctacc 1080

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca 1140

1081 ggcgctctgc cgtggaaatt ggctctggag tagtgggtcc aattctagtt ccagaaaagt 1140

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct 1200

1141 ggaccgggcg tacctgtggg tctggtccag gggatgtagc actggaccct tcggaaccga 1200

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttctt 1260

1201 aaactggggg gagggaccca gttcgggaaa catgtgggat tcggaggcgg aggagaagaa 1260

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccatccgcgc cgtctctccc ccttgaacct cctctttcga ccccgcctca atcctccctt 1320

1261 ggtaggcgcg gcagagaggg ggaacttgga ggagaaagct ggggcggagt taggagggaa 1320

.......... ........ psi_plus_pack ........ .......... ..........

.......... .......... ......... BamHI(1355) ......... ..........

1321 tatccagccc tcactccttc tctaggcgcc ggccggatcc cagtgtggtg gtacgtagga 1380

1321 ataggtcggg agtgaggaag agatccgcgg ccggcctagg gtcacaccac catgcatcct 1380

pBABE_5_primer psi_plus_pack .. .......... .......... ..........

.......... SalI(1397) .......... .......... .......... ..........

1381 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc 1440

1381 taagcggtcg tgtcaccagc tgggacacct tacacacagt caatcccaca cctttcaggg 1440

.......... .......... .... pBABE_3_primer SV40_enhancer SV40_promoter

.......... .......... .......... .......... .......... ..........

1441 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 1500

1441 gtccgagggg tcgtccgtct tcatacgttt cgtacgtaga gttaatcagt cgttggtcca 1500

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 1560

1501 cacctttcag gggtccgagg ggtcgtccgt cttcatacgt ttcgtacgta gagttaatca 1560

.......... . SV40_enhancer SV40_promoter . ........ SV40_promoter

.......... .......... .......... .......... .......... ..........

1561 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 1620

1561 gtcgttggta tcagggcggg gattgaggcg ggtagggcgg ggattgaggc gggtcaaggc 1620

... SV40_enhancer SV40_promoter SV40_promoter SV40_origin ...

.......... .......... .......... .......... .......... ..........

1621 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 1680

1621 gggtaagagg cggggtaccg actgattaaa aaaaataaat acgtctccgg ctccggcgga 1680

SV40_enhancer SV40_promoter SV40_promoter SV40_origin SV40pro_F_primer

.......... .......... .......... .......... StuI(1726) ..........

1681 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 1740

1681 gccggagact cgataaggtc ttcatcactc ctccgaaaaa acctccggat ccgaaaacgt 1740

SV40_promoter .... SV40_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aaaagcttac catgaccgag tacaagccca tgattgaaca agatggattg c acgcaggtt 1800

1741 ttttcgaatg gtactggctc atgttcgggt actaacttgt tctacctaac gtgcgtccaa 1800

.......... .......... ......... Orf frame 3 Neomycin .........

.......... .......... .......... .......... .......... ..........

1801 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct 1860

1801 gaggccggcg aacccacctc tccgataagc cgatactgac ccgtgttgtc tgttagccga 1860

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga 1920

1861 cgagactacg gcggcacaag gccgacagtc gcgtccccgc gggccaagaa aaacagttct 1920

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg 1980

1921 ggctggacag gccacgggac ttacttgacg tcctgctccg tcgcgccgat agcaccgacc 1980

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact 2040

1981 ggtgctgccc gcaaggaacg cgtcgacacg agctgcaaca gtgacttcgc ccttccctga 2040

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg 2100

2041 ccgacgataa cccgcttcac ggccccgtcc tagaggacag tagagtggaa cgaggacggc 2100

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct 2160

2101 tctttcatag gtagtaccga ctacgttacg ccgccgacgt atgcgaacta ggccgatgga 2160

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg 2220

2161 cgggtaagct ggtggttcgc tttgtagcgt agctcgctcg tgcatgagcc taccttcggc 2220

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt 2280

2221 cagaacagct agtcctacta gacctgcttc tcgtagtccc cgagcgcggt cggcttgaca 2280

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg 2340

2281 agcggtccga gttccgcgcg tacgggctgc cgctcctaga gcagcactgg gtaccgctac 2340

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc 2400

2341 ggacgaacgg cttatagtac caccttttac cggcgaaaag acctaagtag ctgacaccgg 2400

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag 2460

2401 ccgacccaca ccgcctggcg atagtcctgt atcgcaaccg atgggcacta taacgacttc 2460

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt 2520

2461 tcgaaccgcc gcttacccga ctggcgaagg agcacgaaat gccatagcgg cgagggctaa 2520

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... ClaI(2567) ..........

2521 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagatcga taaaataaaa 2580

2521 gcgtcgcgta gcggaagata gcggaagaac tgctcaagaa gactctagct attttatttt 2580

.......... ..... Orf frame 3 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa 2640

2581 ctaaaataaa tcagaggtct ttttcccccc ttactttctg gggtggacat ccaaaccgtt 2640

.......... .......... .......... ......... 3MoMuLV_LTR .........

NheI(2641) .......... .......... .......... .......... ..........

2641 gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga gaatagagaa 2700

2641 cgatcgaatt cattgcggta aaacgttccg taccttttta tgtattgact cttatctctt 2700

.......... ......... 3MoMuLV_LTR 5_LTR2 ......... ..........

.......... .......... .......... .......... .......... ..........

2701 gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca ggatatctgt 2760

2701 caagtctagt tccagtcctt gtctaccttg tcgacttata cccggtttgt cctatagaca 2760

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg aatatgggcc 2820

2761 ccattcgtca aggacggggc cgagtcccgg ttcttgtcta ccttgtcgac ttatacccgg 2820

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc 2880

2821 tttgtcctat agacaccatt cgtcaaggac ggggccgagt cccggttctt gtctaccagg 2880

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccagatgcgg tccagccctc agcagtttct agagaaccat cagatgtttc cagggtgccc 2940

2881 ggtctacgcc aggtcgggag tcgtcaaaga tctcttggta gtctacaaag gtcccacggg 2940

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt 3000

2941 gttcctggac tttactggga cacggaataa acttgattgg ttagtcaagc gaagagcgaa 3000

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg 3060

3001 gacaagcgcg cgaagacgag gggctcgagt tattttctcg ggtgttgggg agtgagcccc 3060

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgccagtcct ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc 3120

3061 gcggtcagga ggctaactga ctcagcgggc ccatgggcac ataggttatt tgggagaacg 3120

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact 3180

3121 tcaacgtagg ctgaacacca gagcgacaag gaaccctccc agaggagact cactaactga 3180

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 acccgtcagc gggggtcttt catttccgac ttgtggtctc gctgccttgg gagggtctcc 3240

3181 tgggcagtcg cccccagaaa gtaaaggctg aacaccagag cgacggaacc ctcccagagg 3240

...... 3MoMuLV_LTR 5_LTR2 ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tctgagtgat tgactacccg tcagcggggg tcttcacatg cagcatgtat caaaattaat 3300

3241 agactcacta actgatgggc agtcgccccc agaagtgtac gtcgtacata gttttaatta 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat gaatcggcca 3360

3301 aaccaaaaaa aagaattcat aaatgtaatt taccggtatc aacgtaatta cttagccggt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 acgcgcgggg agaggcggtt tgcgtattgg cgctcttccg cttcctcgct cactgactcg 3420

3361 tgcgcgcccc tctccgccaa acgcataacc gcgagaaggc gaaggagcga gtgactgagc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 3480

3421 gacgcgagcc agcaagccga cgccgctcgc catagtcgag tgagtttccg ccattatgcc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 3540

3481 aataggtgtc ttagtcccct attgcgtcct ttcttgtaca ctcgttttcc ggtcgttttc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 3600

3541 cggtccttgg catttttccg gcgcaacgac cgcaaaaagg tatccgaggc ggggggactg 3600

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3601 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 3660

3601 ctcgtagtgt ttttagctgc gagttcagtc tccaccgctt tgggctgtcc tgatatttct 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 3720

3661 atggtccgca aagggggacc ttcgagggag cacgcgagag gacaaggctg ggacggcgaa 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 3780

3721 tggcctatgg acaggcggaa agagggaagc ccttcgcacc gcgaaagagt atcgagtgcg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 3840

3781 acatccatag agtcaagcca catccagcaa gcgaggttcg acccgacaca cgtgcttggg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 3900

3841 gggcaagtcg ggctggcgac gcggaatagg ccattgatag cagaactcag gttgggccat 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 3960

3901 tctgtgctga atagcggtga ccgtcgtcgg tgaccattgt cctaatcgtc tcgctccata 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 4020

3961 catccgccac gatgtctcaa gaacttcacc accggattga tgccgatgtg atcttcctgt 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 4080

4021 cataaaccat agacgcgaga cgacttcggt caatggaagc ctttttctca accatcgaga 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 4140

4081 actaggccgt ttgtttggtg gcgaccatcg ccaccaaaaa aacaaacgtt cgtcgtctaa 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 4200

4141 tgcgcgtctt tttttcctag agttcttcta ggaaactaga aaagatgccc cagactgcga 4200

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 4260

4201 gtcaccttgc ttttgagtgc aattccctaa aaccagtact ctaatagttt ttcctagaag 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NotI(4294) .......... ..........

4261 acctagatcc ttttaaatta aaaatgaagt ttgcggccgc aaatcaatct aaagtatata 4320

4261 tggatctagg aaaatttaat ttttacttca aacgccggcg tttagttaga tttcatatat 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 4380

4321 actcatttga accagactgt caatggttac gaattagtca ctccgtggat agagtcgcta 4380

.......... .......... .......... .......... Ampicillin ..........

.......... .......... .......... .......... .......... ..........

4381 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 4440

4381 gacagataaa gcaagtaggt atcaacggac tgaggggcag cacatctatt gatgctatgc 4440

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 4500

4441 cctcccgaat ggtagaccgg ggtcacgacg ttactatggc gctctgggtg cgagtggccg 4500

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 4560

4501 aggtctaaat agtcgttatt tggtcggtcg gccttcccgg ctcgcgtctt caccaggacg 4560

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 4620

4561 ttgaaatagg cggaggtagg tcagataatt aacaacggcc cttcgatctc attcatcaag 4620

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 4680

4621 cggtcaatta tcaaacgcgt tgcaacaacg gtaacgatgt ccgtagcacc acagtgcgag 4680

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 4740

4681 cagcaaacca taccgaagta agtcgaggcc aagggttgct agttccgctc aatgtactag 4740

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 4800

4741 ggggtacaac acgttttttc gccaatcgag gaagccagga ggctagcaac agtcttcatt 4800

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 4860

4801 caaccggcgt cacaatagtg agtaccaata ccgtcgtgac gtattaagag aatgacagta 4860

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 4920

4861 cggtaggcat tctacgaaaa gacactgacc actcatgagt tggttcagta agactcttat 4920

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 4980

4921 cacatacgcc gctggctcaa cgagaacggg ccgcagttgt gccctattat ggcgcggtgt 4980

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 5040

4981 atcgtcttga aattttcacg agtagtaacc ttttgcaaga agccccgctt ttgagagttc 5040

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 5100

5041 ctagaatggc gacaactcta ggtcaagcta cattgggtga gcacgtgggt tgactagaag 5100

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 5160

5101 tcgtagaaaa tgaaagtggt cgcaaagacc cactcgtttt tgtccttccg ttttacggcg 5160

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 5220

5161 ttttttccct tattcccgct gtgcctttac aacttatgag tatgagaagg aaaaagttat 5220

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 5280

5221 aataacttcg taaatagtcc caataacaga gtactcgcct atgtataaac ttacataaat 5280

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

5281 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac .......... 5330

5281 ctttttattt gtttatcccc aaggcgcgtg taaaggggct tttcacggtg .......... 5330

.......... .......... .......... .......... .......... ..........