Sequence of pBAD-DEST49

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Sequence of pBAD-DEST49

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Sequence of pBAD-DEST49 : 6160 BP; 1607 A; 1482 C; 1506 G; 1565 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ctagaaataa ttttgtttaa ctttaagaag gagatataca tacccatggg atctgataaa 360

301 gatctttatt aaaacaaatt gaaattcttc ctctatatgt atgggtaccc tagactattt 360

.......... ....... T7_transl_en_RBS ....... ........ His_patch_thio

.......... .......... .......... .......... .......... ..........

361 attattcatc tgactgatga ttcttttgat actgatgtac ttaaggcaga tggtgcaatc 420

361 taataagtag actgactact aagaaaacta tgactacatg aattccgtct accacgttag 420

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctggttgatt tctgggcaca ctggtgcggt ccgtgcaaaa tgatcgctcc gattctggat 480

421 gaccaactaa agacccgtgt gaccacgcca ggcacgtttt actagcgagg ctaagaccta 480

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... ClaI(530) ..........

481 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcacaac 540

481 ctttagcgac tgcttatagt cccgtttgac tggcaacgtt ttgacttgta gctagtgttg 540

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccgggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac 600

541 ggcccgtgac gcggctttat accgtaggca ccatagggct gagacgacga caagtttttg 600

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc 660

601 ccacttcacc gccgttggtt tcacccacgt gacagatttc cagtcaactt tctcaaggag 660

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 gacgctaacc tggccggctc tggatccggt gatgacgatg acaagctggg aattatcaca 720

661 ctgcgattgg accggccgag acctaggcca ctactgctac tgttcgaccc ttaatagtgt 720

His_patch_thio ...... .......... EK .......... ..........

.......... .......... .......... .......... .......... ..........

721 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat 780

721 tcaaacatgt tttttcgact tgctctttgc attttactat atttatagtt atataattta 780

.......... .......... attR1 .......... attR2 ..........

.......... .......... .......... .......... .......... ..........

781 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta 840

781 atctaaaacg tattttttgt ctgatgtatt atgacatttt gtgttgtata ggtcagtgat 840

.......... ......... attR1 attR2 ......... .......... ..........

NotI(844),EagI(844) .......... .......... .......... ..........

841 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt 900

841 accgccggcg taatccgtgg ggtccgaaat gtgaaatacg aaggccgagc atattacaca 900

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggattttgag ttaggatccg gcgagatttt caggagctaa ggaagctaaa atggagaaaa 960

901 cctaaaactc aatcctaggc cgctctaaaa gtcctcgatt ccttcgattt tacctctttt 960

.......... .......... .......... .......... ........ ORF 3 CAT/CamR

.......... .......... .......... .......... .......... ..........

961 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 1020

961 tttagtgacc tatatggtgg caactatata gggttaccgt agcatttctt gtaaaactcc 1020

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct 1080

1021 gtaaagtcag tcaacgagtt acatggatat tggtctggca agtcgaccta taatgccgga 1080

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg 1140

1081 aaaatttctg gcatttcttt ttattcgtgt tcaaaatagg ccggaaataa gtgtaagaac 1140

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... ......... EcoRI(1164) ......... .......... ..........

1141 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga 1200

1141 gggcggacta cttacgagta ggccttaagg cataccgtta ctttctgcca ctcgaccact 1200

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 1260

1201 ataccctatc acaagtggga acaatgtggc aaaaggtact cgtttgactt tgcaaaagta 1260

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 1320

1261 gcgagacctc acttatggtg ctgctaaagg ccgtcaaaga tgtgtatata agcgttctac 1320

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt 1380

1321 accgcacaat gccacttttg gaccggataa agggatttcc caaataactc ttatacaaaa 1380

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg 1440

1381 agcagagtcg gttagggacc cactcaaagt ggtcaaaact aaatttgcac cggttatacc 1440

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc 1500

1441 tgttgaagaa gcgggggcaa aagtggtacc cgtttataat atgcgttccg ctgttccacg 1500

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa 1560

1501 actacggcga ccgctaagtc caagtagtac ggcagacact accgaaggta cagccgtctt 1560

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat 1620

1561 acgaattact taatgttgtc atgacgctac tcaccgtccc gccccgcatt tgcgcaccta 1620

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa 1680

1621 ggccgaatga ttttcggtct attgtcatac gcataaacgc gcgactaaaa acgccatatt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg 1740

1681 cttatatatg actatacata tgggcttcat acagtttttc tccacacgat acttcgtcgc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat 1800

1741 ataatgtcac tgtcaactgt cgctgtcgat agtcaacgag ttccgtatat actacagtta 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc 1860

1801 tagaggccag accattcgtg ttggtacgtc ttacttcggg cagcagacgc acggcttgcg 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc 1920

1861 acctttcgcc ttttagtcct tccctaccga ctccagcggg ccaaataact ttacttgccg 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tcttttgctg acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag 1980

1921 agaaaacgac tgctcttgtc cctgaccact ttacgtcaaa ttccaaatgt ggatattttc 1980

.......... .......... .......... .......... ccdB ..........

.......... .......... .......... .......... .......... ..........

1981 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg 2040

1981 tctctcggca atagcagaca aacacctaca tgtctcacta taataactgt gcgggcccgc 2040

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact 2100

2041 tgcctaccac tagggggacc ggtcacgtgc agacgacagt ctatttcaga gggcacttga 2100

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag 2160

2101 aatgggccac cacgtatagc ccctactttc gaccgcgtac tactggtggc tataccggtc 2160

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat 2220

2161 acacggccag aggcaatagc cccttcttca ccgactagag tcggtggcgc ttttactgta 2220

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccg ttatacacag 2280

2221 gtttttgcgg taattggact acaagacccc ttatatttac agtccgaggc aatatgtgtc 2280

.......... ccdB .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct 2340

2281 ggtcagacgt ccagctggta tcactgacct atacaacaca aaatgtcata atacatcaga 2340

.......... .......... ......... attR2 attR1 ......... ..........

.......... .......... .......... .......... .......... ..........

2341 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt 2400

2341 caaaaaatac gttttagatt aaattatata actataaata tagtaaaatg caaagagcaa 2400

.......... ......... attR2 attR1 ......... .......... ..........

.......... ... BclI(2422),HindIII(2427) ... .......... ..........

2401 cagctttctt gtacaaagtg gtgatcaagc ttgaaggtaa gcctatccct aaccctctcc 2460

2401 gtcgaaagaa catgtttcac cactagttcg aacttccatt cggataggga ttgggagagg 2460

.......... .......... .......... .......... V5 ..........

.......... .......... .......... .......... .......... ..........

2461 tcggtctcga ttctacgcgt accggtcatc atcaccatca ccattgagtt taaacggtct 2520

2461 agccagagct aagatgcgca tggccagtag tagtggtagt ggtaactcaa atttgccaga 2520

V5 .......... .......... 6xHis .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ccagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga 2580

2521 ggtcgaaccg acaaaaccgc ctactctctt ctaaaagtcg gactatgtct aatttagtct 2580

.......... .......... .......... . pBAD_rev_pri pTrcHis_rev_pri .

.......... .......... .......... .......... .......... ..........

2581 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc 2640

2581 tgcgtcttcg ccagactatt ttgtcttaaa cggaccgccg tcatcgcgcc accagggtgg 2640

.......... .......... .......... .......... rrnB_term ..........

.......... .......... .......... .......... .......... ..........

2641 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc 2700

2641 actggggtac ggcttgagtc ttcactttgc ggcatcgcgg ctaccatcac accccagagg 2700

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag t cgaaagact 2760

2701 ggtacgctct catcccttga cggtccgtag tttattttgc tttccgagtc agctttctga 2760

.......... .......... rrnB_term ......... rrnB_T1_term .........

.......... .......... .......... .......... .......... ..........

2761 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc 2820

2761 cccggaaagc aaaatagaca acaaacagcc acttgcgaga ggactcatcc tgtttaggcg 2820

rrnB_T1_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc 2880

2821 gccctcgcct aaacttgcaa cgcttcgttg ccgggcctcc caccgcccgt cctgcgggcg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt 2940

2881 gtatttgacg gtccgtagtt taattcgtct tccggtagga ctgcctaccg gaaaaacgca 2940

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

2941 ttctacaaac tctttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 3000

2941 aagatgtttg agaaaaacaa ataaaaagat ttatgtaagt ttatacatag gcgagtactc 3000

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

3001 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 3060

3001 tgttattggg actatttacg aagttattat aactttttcc ttctcatact cataagttgt 3060

.......... .......... .......... .......... ....... ORF 2 Ampicillin

.......... .......... .......... .......... .......... ..........

3061 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 3120

3061 aaaggcacag cgggaataag ggaaaaaacg ccgtaaaacg gaaggacaaa aacgagtggg 3120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 3180

3121 tctttgcgac cactttcatt ttctacgact tctagtcaac ccacgtgctc acccaatgta 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 3240

3181 gcttgaccta gagttgtcgc cattctagga actctcaaaa gcggggcttc ttgcaaaagg 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg 3300

3241 ttactactcg tgaaaatttc aagacgatac accgcgccat aatagggcac aactgcggcc 3300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 3360

3301 cgttctcgtt gagccagcgg cgtatgtgat aagagtctta ctgaaccaac tcatgagtgg 3360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 3420

3361 tcagtgtctt ttcgtagaat gcctaccgta ctgtcattct cttaatacgt cacgacggta 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 3480

3421 ttggtactca ctattgtgac gccggttgaa tgaagactgt tgctagcctc ctggcttcct 3480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 3540

3481 cgattggcga aaaaacgtgt tgtaccccct agtacattga gcggaactag caacccttgg 3540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 3600

3541 cctcgactta cttcggtatg gtttgctgct cgcactgtgg tgctacggac atcgttaccg 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... FspI(3611) .......... .......... .......... AseI(3659)

3601 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 3660

3601 ttgttgcaac gcgtttgata attgaccgct tgatgaatga gatcgaaggg ccgttgttaa 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 3720

3661 ttatctgacc tacctccgcc tatttcaacg tcctggtgaa gacgcgagcc gggaaggccg 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 3780

3721 accgaccaaa taacgactat ttagacctcg gccactcgca cccagagcgc catagtaacg 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 3840

3781 tcgtgacccc ggtctaccat tcgggagggc atagcatcaa tagatgtgct gcccctcagt 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 3900

3841 ccgttgatac ctacttgctt tatctgtcta gcgactctat ccacggagtg actaattcgt 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 3960

3901 aaccattgac agtctggttc aaatgagtat atatgaaatc taactaaatt ttgaagtaaa 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 4020

3961 aattaaattt tcctagatcc acttctagga aaaactatta gagtactggt tttagggaat 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 4080

4021 tgcactcaaa agcaaggtga ctcgcagtct ggggcatctt ttctagtttc ctagaagaac 4080

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4081 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 4140

4081 tctaggaaaa aaagacgcgc attagacgac gaacgtttgt ttttttggtg gcgatggtcg 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 4200

4141 ccaccaaaca aacggcctag ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 4260

4201 gtctcgcgtc tatggtttat gacaggaaga tcacatcggc atcaatccgg tggtgaagtt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 4320

4261 cttgagacat cgtggcggat gtatggagcg agacgattag gacaatggtc accgacgacg 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 4380

4321 gtcaccgcta ttcagcacag aatggcccaa cctgagttct gctatcaatg gcctattccg 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 4440

4381 cgtcgccagc ccgacttgcc ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 4500

4441 gtggcttgac tctatggatg tcgcactcga tactctttcg cggtgcgaag ggcttccctc 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 4560

4501 tttccgcctg tccataggcc attcgccgtc ccagccttgt cctctcgcgt gctccctcga 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 4620

4561 aggtccccct ttgcggacca tagaaatatc aggacagccc aaagcggtgg agactgaact 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 4680

4621 cgcagctaaa aacactacga gcagtccccc cgcctcggat acctttttgc ggtcgttgcg 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 4740

4681 ccggaaaaat gccaaggacc ggaaaacgac cggaaaacga gtgtacaaga aaggacgcaa 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 4800

4741 taggggacta agacacctat tggcataatg gcggaaactc actcgactat ggcgagcggc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg 4860

4801 gtcggcttgc tggctcgcgt cgctcagtca ctcgctcctt cgccttctcg cggactacgc 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NdeI(4901) ..........

4861 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac 4920

4861 cataaaagag gaatgcgtag acacgccata aagtgtggcg tataccacgt gagagtcatg 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg 4980

4921 ttagacgaga ctacggcgta tcaattcggt catatgtgag gcgatagcga tgcactgacc 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 5040

4981 cagtaccgac gcggggctgt gggcggttgt gggcgactgc gcgggactgc ccgaacagac 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 5100

5041 gagggccgta ggcgaatgtc tgttcgacac tggcagaggc cctcgacgta cacagtctcc 5100

.......... .......... .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

5101 ttttcaccgt catcaccgaa acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag 5160

5101 aaaagtggca gtagtggctt tgcgcgctcc gtcgtctagt taagcgcgcg cttccgcttc 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cggcatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 5220

5161 gccgtacgta ttacacggac agtttacctg cttcgtccct aagacgtttg ggatacgatg 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 5280

5221 aggcagttcg gcagttaaca gactaagcaa tggttaatac tgttgaactg ccgatgtagt 5280

.......... .......... .......... .......... AraC ..........

.......... .......... .......... .......... .......... ..........

5281 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 5340

5281 aagtgaaaaa gaagtgttgg ccgtgccttg agcgagcccg accggggcca cgtaaaaaat 5340

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 5400

5341 ttatgggcgc tctttatctc aactagcagt tttggttgta acgctggctg ccaccgctat 5400

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 5460

5401 ccgtaggccc accacgagtt ttcgtcgaag cggaccgact atgcaaccag gagcgcggtc 5460

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 5520

5461 gaattctgcg attagggatt gacgaccgcc ttttctacac tgtctgcgct gccgctgttc 5520

.......... .......... AraC .......... .......... ..........

.......... ......... EcoRV(5546) ......... .......... ..........

5521 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 5580

5521 gtttgtacga cacgctgcga ccgctatagt tttaacgaca gacggtccac tagcgactac 5580

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 5640

5581 atgactgttc ggagcgcatg ggctaatagg tagccaccta cctcgctgag caattagcga 5640

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 5700

5641 aggtacgcgg cgtcattgtt aacgagttcg tctaaatagc ggtcgtcgag gcttatcgcg 5700

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 5760

5701 ggaaggggaa cgggccgcaa ttactaaacg ggtttgtcca gcgactttac gccgaccacg 5760

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 5820

5761 cgaagtaggc ccgctttctt ggggcataac cgtttataac tgccggtcaa ttcggtaagt 5820

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... NruI(5868) ..........

5821 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 5880

5821 acggtcatcc gcgcgcctgc tttcatttgg gtgaccacta tggtaagcgc tcggaggcct 5880

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 5940

5881 actgctggca tcactactta gagaggaccg cccttgtcgt tttatagtgg gccagccgtt 5940

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 6000

5941 tgtttaagag cagggactaa aaagtggtgg gggactggcg cttaccactc taactcttat 6000

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 6060

6001 attggaaagt aagggtcgcc agccagctat ttttttagct ctattggcaa ccggagttag 6060

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 6120

6061 ccgcaatttg ggcggtggtc tacccgtaat ttgctcatag ggccgtcgtc ccctagtaaa 6120

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tgcgcttcag ccatactttt catactcccg ccattcagag .......... .......... 6160

6121 acgcgaagtc ggtatgaaaa gtatgagggc ggtaagtctc .......... .......... 6160

AraC .......... .......... .......... .......... ..........