LOCUS pBAD-TOPO 4127 bp DNA SYN 29-Oct-2007 DEFINITION pBAD-TOPO ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4127 /organism="pBAD-TOPO" /mol_type="other DNA" CDS complement(3223..25) /label="ORF frame 1" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 4..19 /label="araO2" misc_feature 161..172 /label="araO1" misc_feature 203..216 /label="CAP_BS" misc_feature 208..227 /label="pBAD_fwd_primer" misc_feature 213..251 /label="AraI1I2" promoter 248..276 /label="ARA_promoter" misc_feature 317..333 /label="T7_transl_en_RBS" misc_feature 364..378 /label="EK" misc_feature 403..444 /label="V5" misc_feature 454..471 /label="6xHis" misc_feature complement(527..544) /label="pBAD_rev_primer" misc_feature complement(527..544) /label="pTrcHis_rev_primer" terminator 577..734 /label="rrnB_terminator" terminator 700..743 /label="rrnB_T1_terminator" terminator 875..902 /label="rrnB_T2_terminator" promoter 944..972 /label="AmpR_promoter" CDS 1014..1874 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 1014..1874 /label="Ampicillin" /gene="Ampicillin" rep_origin 2029..2648 /label="pBR322_origin" misc_feature 3045..3067 /label="pGEX_3_primer" misc_feature complement(3223..4101) /label="AraC" ORIGIN 1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT 61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA 121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG 181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG 241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG 301 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TACCCATGGG CTCTGGATCC 361 GGTGATGACG ATGACAAGCT CGCCCTTAAG GGCGAGCTTG AAGGTAAGCC TATCCCTAAC 421 CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC ACCATCACCA TTGAGTTTAA 481 ACGGTCTCCA GCTTGGCTGT TTTGGCGGAT GAGAGAAGAT TTTCAGCCTG ATACAGATTA 541 AATCAGAACG CAGAAGCGGT CTGATAAAAC AGAATTTGCC TGGCGGCAGT AGCGCGGTGG 601 TCCCACCTGA CCCCATGCCG AACTCAGAAG TGAAACGCCG TAGCGCCGAT GGTAGTGTGG 661 GGTCTCCCCA TGCGAGAGTA GGGAACTGCC AGGCATCAAA TAAAACGAAA GGCTCAGTCG 721 AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA ACGCTCTCCT GAGTAGGACA 781 AATCCGCCGG GAGCGGATTT GAACGTTGCG AAGCAACGGC CCGGAGGGTG GCGGGCAGGA 841 CGCCCGCCAT AAACTGCCAG GCATCAAATT AAGCAGAAGG CCATCCTGAC GGATGGCCTT 901 TTTGCGTTTC TACAAACTCT TTTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC 961 TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA 1021 TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG 1081 CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG 1141 GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC 1201 GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG 1261 ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT 1321 ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG 1381 CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC 1441 CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT 1501 GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG 1561 CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 1621 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 1681 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 1741 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 1801 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 1861 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC 1921 TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA 1981 TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT 2041 CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC 2101 TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG 2161 GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC 2221 ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG 2281 CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG 2341 ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA 2401 CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG 2461 AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA 2521 GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT 2581 GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA 2641 GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC 2701 CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG 2761 CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC 2821 TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC 2881 TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG 2941 TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC 3001 TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG 3061 TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CAGATCAATT CGCGCGCGAA 3121 GGCGAAGCGG CATGCATAAT GTGCCTGTCA AATGGACGAA GCAGGGATTC TGCAAACCCT 3181 ATGCTACTCC GTCAAGCCGT CAATTGTCTG ATTCGTTACC AATTATGACA ACTTGACGGC 3241 TACATCATTC ACTTTTTCTT CACAACCGGC ACGGAACTCG CTCGGGCTGG CCCCGGTGCA 3301 TTTTTTAAAT ACCCGCGAGA AATAGAGTTG ATCGTCAAAA CCAACATTGC GACCGACGGT 3361 GGCGATAGGC ATCCGGGTGG TGCTCAAAAG CAGCTTCGCC TGGCTGATAC GTTGGTCCTC 3421 GCGCCAGCTT AAGACGCTAA TCCCTAACTG CTGGCGGAAA AGATGTGACA GACGCGACGG 3481 CGACAAGCAA ACATGCTGTG CGACGCTGGC GATATCAAAA TTGCTGTCTG CCAGGTGATC 3541 GCTGATGTAC TGACAAGCCT CGCGTACCCG ATTATCCATC GGTGGATGGA GCGACTCGTT 3601 AATCGCTTCC ATGCGCCGCA GTAACAATTG CTCAAGCAGA TTTATCGCCA GCAGCTCCGA 3661 ATAGCGCCCT TCCCCTTGCC CGGCGTTAAT GATTTGCCCA AACAGGTCGC TGAAATGCGG 3721 CTGGTGCGCT TCATCCGGGC GAAAGAACCC CGTATTGGCA AATATTGACG GCCAGTTAAG 3781 CCATTCATGC CAGTAGGCGC GCGGACGAAA GTAAACCCAC TGGTGATACC ATTCGCGAGC 3841 CTCCGGATGA CGACCGTAGT GATGAATCTC TCCTGGCGGG AACAGCAAAA TATCACCCGG 3901 TCGGCAAACA AATTCTCGTC CCTGATTTTT CACCACCCCC TGACCGCGAA TGGTGAGATT 3961 GAGAATATAA CCTTTCATTC CCAGCGGTCG GTCGATAAAA AAATCGAGAT AACCGTTGGC 4021 CTCAATCGGC GTTAAACCCG CCACCAGATG GGCATTAAAC GAGTATCCCG GCAGCAGGGG 4081 ATCATTTTGC GCTTCAGCCA TACTTTTCAT ACTCCCGCCA TTCAGAG // pBAD-TOPO "Times New RomanC ORF 1"Times New Roman(C AraC"Times New RomanCaraO2"Times New RomanCɆaraO1"Times New RomanCAP_BS"Times New RomanC pBAD_fwd_pri"Times New RomanCAraI1I2"Times New Roman*CɆARA_pro"Times New Roman=MT7_transl_en_RBS"Times New RomanClzEK"Times New RomanCV5"Times New RomanCɆ6xHis"Times New Roman  pBAD_rev_pri"Times New RomanC pTrcHis_rev_pri"Times New RomanCA rrnB_term"Times New RomanCɆ rrnB_T1_term"Times New Romank rrnB_T2_term"Times New RomanCAmpR_pro"Times New RomanCRORF 3"Times New RomanCɆR Ampicillin"Times New RomanX  pBR322_origin"Times New RomanC  pGEX_3_pri"Times New Roman NcoI"Times New RomanXFP@@ FspI(1578),AseI(1626)"Times New Roman*lxz NdeI"Times New Roman4 %, EcoRV"Times New Roman @ 4'' NruI"Times New RomanumdE test"Times New Roman]]ff? test"Times New RomanuuP