**5LOCUS pBAD-TOPO/LacZ/V5-His 7183 bp DNA SYN 22-Oct-2007 DEFINITION pBAD-TOPO/LacZ/V5-His ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7183 /organism="pBAD-TOPO/LacZ/V5-His" /mol_type="other DNA" CDS complement(6279..25) /label="ORF frame 3" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 4..19 /label="araO2" misc_feature 161..172 /label="araO1" misc_feature 203..216 /label="CAP_BS" misc_feature 208..227 /label="pBAD_fwd_primer" misc_feature 213..251 /label="AraI1I2" promoter 248..276 /label="ARA_promoter" misc_feature 316..332 /label="T7_transl_en_RBS" misc_feature 363..377 /label="EK" misc_feature complement(401..423) /label="M13_pUC_fwd_primer" gene 399..3443 /label="lacZ" /gene="lacZ" CDS 345..3530 /label="lacZ" /gene="lacZ" /note="ORF frame 3" /translation="MGSGSGDDDDKLALMIDPVVLQRRDWENPGVTQLNRLAAHPPFA SWRNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQM HGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAF HLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGI FRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQ VASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIE AEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQN NFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERV TRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDI ICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAF RQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPH PALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDV APQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTL PAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAP LDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQG KTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQE NYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRY SQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVW CQKKGELEGKPIPNPLLGLDSTRTGHHHHHH*" misc_feature 3459..3500 /label="V5" misc_feature 3510..3527 /label="6xHis" misc_feature complement(3583..3600) /label="pBAD_rev_primer" misc_feature complement(3583..3600) /label="pTrcHis_rev_primer" terminator 3633..3790 /label="rrnB_terminator" terminator 3756..3799 /label="rrnB_T1_terminator" terminator 3931..3958 /label="rrnB_T2_terminator" promoter 4000..4028 /label="AmpR_promoter" CDS 4070..4930 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 4070..4930 /label="Ampicillin" /gene="Ampicillin" rep_origin 5085..5704 /label="pBR322_origin" misc_feature 6101..6123 /label="pGEX_3_primer" misc_feature complement(6279..7157) /label="AraC" ORIGIN 1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT 61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA 121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG 181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG 241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGC 301 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ACCCATGGGC TCTGGATCCG 361 GTGATGACGA TGACAAGCTC GCCCTTATGA TAGATCCCGT CGTTTTACAA CGTCGTGACT 421 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT 481 GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG 541 GCGAATGGCG CTTTGCCTGG TTTCCGGCAC CAGAAGCGGT GCCGGAAAGC TGGCTGGAGT 601 GCGATCTTCC TGAGGCCGAT ACTGTCGTCG TCCCCTCAAA CTGGCAGATG CACGGTTACG 661 ATGCGCCCAT CTACACCAAC GTGACCTATC CCATTACGGT CAATCCGCCG TTTGTTCCCA 721 CGGAGAATCC GACGGGTTGT TACTCGCTCA CATTTAATGT TGATGAAAGC TGGCTACAGG 781 AAGGCCAGAC GCGAATTATT TTTGATGGCG TTAACTCGGC GTTTCATCTG TGGTGCAACG 841 GGCGCTGGGT CGGTTACGGC CAGGACAGTC GTTTGCCGTC TGAATTTGAC CTGAGCGCAT 901 TTTTACGCGC CGGAGAAAAC CGCCTCGCGG TGATGGTGCT GCGTTGGAGT GACGGCAGTT 961 ATCTGGAAGA TCAGGATATG TGGCGGATGA GCGGCATTTT CCGTGACGTC TCGTTGCTGC 1021 ATAAACCGAC TACACAAATC AGCGATTTCC ATGTTGCCAC TCGCTTTAAT GATGATTTCA 1081 GCCGCGCTGT ACTGGAGGCT GAAGTTCAGA TGTGCGGCGA GTTGCGTGAC TACCTACGGG 1141 TAACAGTTTC TTTATGGCAG GGTGAAACGC AGGTCGCCAG CGGCACCGCG CCTTTCGGCG 1201 GTGAAATTAT CGATGAGCGT GGTGGTTATG CCGATCGCGT CACACTACGT CTGAACGTCG 1261 AAAACCCGAA ACTGTGGAGC GCCGAAATCC CGAATCTCTA TCGTGCGGTG GTTGAACTGC 1321 ACACCGCCGA CGGCACGCTG ATTGAAGCAG AAGCCTGCGA TGTCGGTTTC CGCGAGGTGC 1381 GGATTGAAAA TGGTCTGCTG CTGCTGAACG GCAAGCCGTT GCTGATTCGA GGCGTTAACC 1441 GTCACGAGCA TCATCCTCTG CATGGTCAGG TCATGGATGA GCAGACGATG GTGCAGGATA 1501 TCCTGCTGAT GAAGCAGAAC AACTTTAACG CCGTGCGCTG TTCGCATTAT CCGAACCATC 1561 CGCTGTGGTA CACGCTGTGC GACCGCTACG GCCTGTATGT GGTGGATGAA GCCAATATTG 1621 AAACCCACGG CATGGTGCCA ATGAATCGTC TGACCGATGA TCCGCGCTGG CTACCGGCGA 1681 TGAGCGAACG CGTAACGCGA ATGGTGCAGC GCGATCGTAA TCACCCGAGT GTGATCATCT 1741 GGTCGCTGGG GAATGAATCA GGCCACGGCG CTAATCACGA CGCGCTGTAT CGCTGGATCA 1801 AATCTGTCGA TCCTTCCCGC CCGGTGCAGT ATGAAGGCGG CGGAGCCGAC ACCACGGCCA 1861 CCGATATTAT TTGCCCGATG TACGCGCGCG TGGATGAAGA CCAGCCCTTC CCGGCTGTGC 1921 CGAAATGGTC CATCAAAAAA TGGCTTTCGC TACCTGGAGA GACGCGCCCG CTGATCCTTT 1981 GCGAATACGC CCACGCGATG GGTAACAGTC TTGGCGGTTT CGCTAAATAC TGGCAGGCGT 2041 TTCGTCAGTA TCCCCGTTTA CAGGGCGGCT TCGTCTGGGA CTGGGTGGAT CAGTCGCTGA 2101 TTAAATATGA TGAAAACGGC AACCCGTGGT CGGCTTACGG CGGTGATTTT GGCGATACGC 2161 CGAACGATCG CCAGTTCTGT ATGAACGGTC TGGTCTTTGC CGACCGCACG CCGCATCCAG 2221 CGCTGACGGA AGCAAAACAC CAGCAGCAGT TTTTCCAGTT CCGTTTATCC GGGCAAACCA 2281 TCGAAGTGAC CAGCGAATAC CTGTTCCGTC ATAGCGATAA CGAGCTCCTG CACTGGATGG 2341 TGGCGCTGGA TGGTAAGCCG CTGGCAAGCG GTGAAGTGCC TCTGGATGTC GCTCCACAAG 2401 GTAAACAGTT GATTGAACTG CCTGAACTAC CGCAGCCGGA GAGCGCCGGG CAACTCTGGC 2461 TCACAGTACG CGTAGTGCAA CCGAACGCGA CCGCATGGTC AGAAGCCGGG CACATCAGCG 2521 CCTGGCAGCA GTGGCGTCTG GCGGAAAACC TCAGTGTGAC GCTCCCCGCC GCGTCCCACG 2581 CCATCCCGCA TCTGACCACC AGCGAAATGG ATTTTTGCAT CGAGCTGGGT AATAAGCGTT 2641 GGCAATTTAA CCGCCAGTCA GGCTTTCTTT CACAGATGTG GATTGGCGAT AAAAAACAAC 2701 TGCTGACGCC GCTGCGCGAT CAGTTCACCC GTGCACCGCT GGATAACGAC ATTGGCGTAA 2761 GTGAAGCGAC CCGCATTGAC CCTAACGCCT GGGTCGAACG CTGGAAGGCG GCGGGCCATT 2821 ACCAGGCCGA AGCAGCGTTG TTGCAGTGCA CGGCAGATAC ACTTGCTGAT GCGGTGCTGA 2881 TTACGACCGC TCACGCGTGG CAGCATCAGG GGAAAACCTT ATTTATCAGC CGGAAAACCT 2941 ACCGGATTGA TGGTAGTGGT CAAATGGCGA TTACCGTTGA TGTTGAAGTG GCGAGCGATA 3001 CACCGCATCC GGCGCGGATT GGCCTGAACT GCCAGCTGGC GCAGGTAGCA GAGCGGGTAA 3061 ACTGGCTCGG ATTAGGGCCG CAAGAAAACT ATCCCGACCG CCTTACTGCC GCCTGTTTTG 3121 ACCGCTGGGA TCTGCCATTG TCAGACATGT ATACCCCGTA CGTCTTCCCG AGCGAAAACG 3181 GTCTGCGCTG CGGGACGCGC GAATTGAATT ATGGCCCACA CCAGTGGCGC GGCGACTTCC 3241 AGTTCAACAT CAGCCGCTAC AGTCAACAGC AACTGATGGA AACCAGCCAT CGCCATCTGC 3301 TGCACGCGGA AGAAGGCACA TGGCTGAATA TCGACGGTTT CCATATGGGG ATTGGTGGCG 3361 ACGACTCCTG GAGCCCGTCA GTATCGGCGG AATTCCAGCT GAGCGCCGGT CGCTACCATT 3421 ACCAGTTGGT CTGGTGTCAA AAAAAGGGCG AGCTTGAAGG TAAGCCTATC CCTAACCCTC 3481 TCCTCGGTCT CGATTCTACG CGTACCGGTC ATCATCACCA TCACCATTGA GTTTAAACGG 3541 TCTCCAGCTT GGCTGTTTTG GCGGATGAGA GAAGATTTTC AGCCTGATAC AGATTAAATC 3601 AGAACGCAGA AGCGGTCTGA TAAAACAGAA TTTGCCTGGC GGCAGTAGCG CGGTGGTCCC 3661 ACCTGACCCC ATGCCGAACT CAGAAGTGAA ACGCCGTAGC GCCGATGGTA GTGTGGGGTC 3721 TCCCCATGCG AGAGTAGGGA ACTGCCAGGC ATCAAATAAA ACGAAAGGCT CAGTCGAAAG 3781 ACTGGGCCTT TCGTTTTATC TGTTGTTTGT CGGTGAACGC TCTCCTGAGT AGGACAAATC 3841 CGCCGGGAGC GGATTTGAAC GTTGCGAAGC AACGGCCCGG AGGGTGGCGG GCAGGACGCC 3901 CGCCATAAAC TGCCAGGCAT CAAATTAAGC AGAAGGCCAT CCTGACGGAT GGCCTTTTTG 3961 CGTTTCTACA AACTCTTTTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT 4021 GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA 4081 ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA 4141 CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA 4201 CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT 4261 TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC 4321 CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC 4381 ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC 4441 CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA 4501 GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA 4561 ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT 4621 GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA 4681 ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC 4741 GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT 4801 TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG 4861 TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA 4921 GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA 4981 TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC 5041 TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 5101 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 5161 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 5221 CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 5281 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 5341 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 5401 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 5461 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 5521 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 5581 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 5641 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 5701 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC 5761 GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG 5821 CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT 5881 GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT GCACTCTCAG 5941 TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC 6001 TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT 6061 CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG 6121 AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCAGA TCAATTCGCG CGCGAAGGCG 6181 AAGCGGCATG CATAATGTGC CTGTCAAATG GACGAAGCAG GGATTCTGCA AACCCTATGC 6241 TACTCCGTCA AGCCGTCAAT TGTCTGATTC GTTACCAATT ATGACAACTT GACGGCTACA 6301 TCATTCACTT TTTCTTCACA ACCGGCACGG AACTCGCTCG GGCTGGCCCC GGTGCATTTT 6361 TTAAATACCC GCGAGAAATA GAGTTGATCG TCAAAACCAA CATTGCGACC GACGGTGGCG 6421 ATAGGCATCC GGGTGGTGCT CAAAAGCAGC TTCGCCTGGC TGATACGTTG GTCCTCGCGC 6481 CAGCTTAAGA CGCTAATCCC TAACTGCTGG CGGAAAAGAT GTGACAGACG CGACGGCGAC 6541 AAGCAAACAT GCTGTGCGAC GCTGGCGATA TCAAAATTGC TGTCTGCCAG GTGATCGCTG 6601 ATGTACTGAC AAGCCTCGCG TACCCGATTA TCCATCGGTG GATGGAGCGA CTCGTTAATC 6661 GCTTCCATGC GCCGCAGTAA CAATTGCTCA AGCAGATTTA TCGCCAGCAG CTCCGAATAG 6721 CGCCCTTCCC CTTGCCCGGC GTTAATGATT TGCCCAAACA GGTCGCTGAA ATGCGGCTGG 6781 TGCGCTTCAT CCGGGCGAAA GAACCCCGTA TTGGCAAATA TTGACGGCCA GTTAAGCCAT 6841 TCATGCCAGT AGGCGCGCGG ACGAAAGTAA ACCCACTGGT GATACCATTC GCGAGCCTCC 6901 GGATGACGAC CGTAGTGATG AATCTCTCCT GGCGGGAACA GCAAAATATC ACCCGGTCGG 6961 CAAACAAATT CTCGTCCCTG ATTTTTCACC ACCCCCTGAC CGCGAATGGT GAGATTGAGA 7021 ATATAACCTT TCATTCCCAG CGGTCGGTCG ATAAAAAAAT CGAGATAACC GTTGGCCTCA 7081 ATCGGCGTTA AACCCGCCAC CAGATGGGCA TTAAACGAGT ATCCCGGCAG CAGGGGATCA 7141 TTTTGCGCTT CAGCCATACT TTTCATACTC CCGCCATTCA GAG // pBAD-TOPO/LacZ/V5-His "Times New Roman$*C ORF 3"Times New Roman*C araO2"Times New Roman*CɆ araO1"Times New Roman* CAP_BS"Times New Roman*C  pBAD_fwd_pri"Times New Roman*C AraI1I2"Times New Roman**CɆ ARA_pro"Times New Roman* <LT7_transl_en_RBS"Times New Roman* Y ORF 3"Times New Roman*C kyEK"Times New Roman*CɆ s lacZ"Times New Roman*C M13_pUC_fwd_pri"Times New Roman)*C  V5"Times New Roman*C  6xHis"Times New Roman*CɆ   pBAD_rev_pri"Times New Roman*  pTrcHis_rev_pri"Times New Roman*C 1 rrnB_term"Times New Roman*C  rrnB_T1_term"Times New Roman*CɆ [v rrnB_T2_term"Times New Roman* AmpR_pro"Times New Roman*C BORF 2"Times New Roman*C B Ampicillin"Times New Roman*CɆ H pBR322_origin"Times New Roman*  pGEX_3_pri"Times New Roman*C AraC"Times New Roman *NcoI"Times New RomanW 4  % B  *AatII"Times New Roman |obSb *ClaI"Times New Roman z *BclI"Times New Roman  *SacI"Times New Roman  t}~ *EcoRI"Times New Roman>   ,' *AseI"Times New RomanJ   *AflII"Times New RomanT OUDE84%84 *NruI"Times New Roman 2 m  *test"Times New Roman ,L,h *test"Times New Roman ,