Sequence of pBAD-TOPO_LacZ

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Sequence of pBAD-TOPO_LacZ_V5-His

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Sequence of pBAD-TOPO/LacZ/V5-His : 7183 BP; 1711 A; 1900 C; 1903 G; 1669 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaacccg 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... .......... .......... .......... NcoI(343) ..........

301 tagaaataat tttgtttaac tttaagaagg agatatacat acccatgggc tctggatccg 360

301 atctttatta aaacaaattg aaattcttcc tctatatgta tgggtacccg agacctaggc 360

.......... ....... T7_transl_en_RBS ....... .......... ORF 3

.......... .......... .......... .......... .......... ..........

361 gtgatgacga tgacaagctc gcccttatga tagatcccgt cgttttacaa cgtcgtgact 420

361 cactactgct actgttcgag cgggaatact atctagggca gcaaaatgtt gcagcactga 420

EK .......... ORF 3 ..... lacZ M13_pUC_fwd_pri .....

.......... .......... .......... .......... .......... ..........

421 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 480

421 cccttttggg accgcaatgg gttgaattag cggaacgtcg tgtaggggga aagcggtcga 480

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 540

481 ccgcattatc gcttctccgg gcgtggctag cgggaagggt tgtcaacgcg tcggacttac 540

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt 600

541 cgcttaccgc gaaacggacc aaaggccgtg gtcttcgcca cggcctttcg accgacctca 600

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg 660

601 cgctagaagg actccggcta tgacagcagc aggggagttt gaccgtctac gtgccaatgc 660

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 atgcgcccat ctacaccaac gtgacctatc ccattacggt caatccgccg tttgttccca 720

661 tacgcgggta gatgtggttg cactggatag ggtaatgcca gttaggcggc aaacaagggt 720

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg 780

721 gcctcttagg ctgcccaaca atgagcgagt gtaaattaca actactttcg accgatgtcc 780

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aaggccagac gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg 840

781 ttccggtctg cgcttaataa aaactaccgc aattgagccg caaagtagac accacgttgc 840

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggcgctgggt cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat 900

841 ccgcgaccca gccaatgccg gtcctgtcag caaacggcag acttaaactg gactcgcgta 900

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct gcgttggagt gacggcagtt 960

901 aaaatgcgcg gcctcttttg gcggagcgcc actaccacga cgcaacctca ctgccgtcaa 960

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... ......... AatII(1009) .........

961 atctggaaga tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc 1020

961 tagaccttct agtcctatac accgcctact cgccgtaaaa ggcactgcag agcaacgacg 1020

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ataaaccgac tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca 1080

1021 tatttggctg atgtgtttag tcgctaaagg tacaacggtg agcgaaatta ctactaaagt 1080

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gccgcgctgt actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg 1140

1081 cggcgcgaca tgacctccga cttcaagtct acacgccgct caacgcactg atggatgccc 1140

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 taacagtttc tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg 1200

1141 attgtcaaag aaataccgtc ccactttgcg tccagcggtc gccgtggcgc ggaaagccgc 1200

.......... .......... ORF 3 lacZ .......... .......... ..........

ClaI(1210) .......... .......... .......... .......... ..........

1201 gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg 1260

1201 cactttaata gctactcgca ccaccaatac ggctagcgca gtgtgatgca gacttgcagc 1260

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aaaacccgaa actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc 1320

1261 ttttgggctt tgacacctcg cggctttagg gcttagagat agcacgccac caacttgacg 1320

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acaccgccga cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc 1380

1321 tgtggcggct gccgtgcgac taacttcgtc ttcggacgct acagccaaag gcgctccacg 1380

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc 1440

1381 cctaactttt accagacgac gacgacttgc cgttcggcaa cgactaagct ccgcaattgg 1440

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gtcacgagca tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata 1500

1441 cagtgctcgt agtaggagac gtaccagtcc agtacctact cgtctgctac cacgtcctat 1500

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tcctgctgat gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc 1560

1501 aggacgacta cttcgtcttg ttgaaattgc ggcacgcgac aagcgtaata ggcttggtag 1560

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg 1620

1561 gcgacaccat gtgcgacacg ctggcgatgc cggacataca ccacctactt cggttataac 1620

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aaacccacgg catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga 1680

1621 tttgggtgcc gtaccacggt tacttagcag actggctact aggcgcgacc gatggccgct 1680

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1732)

1681 tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct 1740

1681 actcgcttgc gcattgcgct taccacgtcg cgctagcatt agtgggctca cactagtaga 1740

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggtcgctggg gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca 1800

1741 ccagcgaccc cttacttagt ccggtgccgc gattagtgct gcgcgacata gcgacctagt 1800

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca 1860

1801 ttagacagct aggaagggcg ggccacgtca tacttccgcc gcctcggctg tggtgccggt 1860

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc 1920

1861 ggctataata aacgggctac atgcgcgcgc acctacttct ggtcgggaag ggccgacacg 1920

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cgaaatggtc catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt 1980

1921 gctttaccag gtagtttttt accgaaagcg atggacctct ctgcgcgggc gactaggaaa 1980

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt 2040

1981 cgcttatgcg ggtgcgctac ccattgtcag aaccgccaaa gcgatttatg accgtccgca 2040

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttcgtcagta tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga 2100

2041 aagcagtcat aggggcaaat gtcccgccga agcagaccct gacccaccta gtcagcgact 2100

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttaaatatga tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc 2160

2101 aatttatact acttttgccg ttgggcacca gccgaatgcc gccactaaaa ccgctatgcg 2160

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgaacgatcg ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag 2220

2161 gcttgctagc ggtcaagaca tacttgccag accagaaacg gctggcgtgc ggcgtaggtc 2220

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgctgacgga agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca 2280

2221 gcgactgcct tcgttttgtg gtcgtcgtca aaaaggtcaa ggcaaatagg cccgtttggt 2280

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... SacI(2326) ..........

2281 tcgaagtgac cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg 2340

2281 agcttcactg gtcgcttatg gacaaggcag tatcgctatt gctcgaggac gtgacctacc 2340

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag 2400

2341 accgcgacct accattcggc gaccgttcgc cacttcacgg agacctacag cgaggtgttc 2400

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtaaacagtt gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc 2460

2401 catttgtcaa ctaacttgac ggacttgatg gcgtcggcct ctcgcggccc gttgagaccg 2460

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg 2520

2461 agtgtcatgc gcatcacgtt ggcttgcgct ggcgtaccag tcttcggccc gtgtagtcgc 2520

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg 2580

2521 ggaccgtcgt caccgcagac cgccttttgg agtcacactg cgaggggcgg cgcagggtgc 2580

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccatcccgca tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt 2640

2581 ggtagggcgt agactggtgg tcgctttacc taaaaacgta gctcgaccca ttattcgcaa 2640

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggcaatttaa ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac 2700

2641 ccgttaaatt ggcggtcagt ccgaaagaaa gtgtctacac ctaaccgcta ttttttgttg 2700

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa 2760

2701 acgactgcgg cgacgcgcta gtcaagtggg cacgtggcga cctattgctg taaccgcatt 2760

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt 2820

2761 cacttcgctg ggcgtaactg ggattgcgga cccagcttgc gaccttccgc cgcccggtaa 2820

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 accaggccga agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga 2880

2821 tggtccggct tcgtcgcaac aacgtcacgt gccgtctatg tgaacgacta cgccacgact 2880

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct 2940

2881 aatgctggcg agtgcgcacc gtcgtagtcc ccttttggaa taaatagtcg gccttttgga 2940

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 accggattga tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata 3000

2941 tggcctaact accatcacca gtttaccgct aatggcaact acaacttcac cgctcgctat 3000

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 caccgcatcc ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa 3060

3001 gtggcgtagg ccgcgcctaa ccggacttga cggtcgaccg cgtccatcgt ctcgcccatt 3060

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 actggctcgg attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg 3120

3061 tgaccgagcc taatcccggc gttcttttga tagggctggc ggaatgacgg cggacaaaac 3120

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 accgctggga tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg 3180

3121 tggcgaccct agacggtaac agtctgtaca tatggggcat gcagaagggc tcgcttttgc 3180

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtctgcgctg cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc 3240

3181 cagacgcgac gccctgcgcg cttaacttaa taccgggtgt ggtcaccgcg ccgctgaagg 3240

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agttcaacat cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc 3300

3241 tcaagttgta gtcggcgatg tcagttgtcg ttgactacct ttggtcggta gcggtagacg 3300

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgcacgcgga agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg 3360

3301 acgtgcgcct tcttccgtgt accgacttat agctgccaaa ggtatacccc taaccaccgc 3360

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... ......... EcoRI(3390) ......... .......... ..........

3361 acgactcctg gagcccgtca gtatcggcgg aattccagct gagcgccggt cgctaccatt 3420

3361 tgctgaggac ctcgggcagt catagccgcc ttaaggtcga ctcgcggcca gcgatggtaa 3420

.......... .......... ORF 3 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 accagttggt ctggtgtcaa aaaaagggcg agcttgaagg taagcctatc cctaaccctc 3480

3421 tggtcaacca gaccacagtt tttttcccgc tcgaacttcc attcggatag ggattgggag 3480

.......... lacZ ORF 3 .......... V5 ..........

.......... .......... .......... .......... .......... ..........

3481 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaacgg 3540

3481 aggagccaga gctaagatgc gcatggccag tagtagtggt agtggtaact caaatttgcc 3540

V5 .......... ORF 3 6xHis .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tctccagctt ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc 3600

3541 agaggtcgaa ccgacaaaac cgcctactct cttctaaaag tcggactatg tctaatttag 3600

.......... .......... .......... .......... . pBAD_rev_pri pTrcHis_rev_pri

.......... .......... .......... .......... .......... ..........

3601 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc 3660

3601 tcttgcgtct tcgccagact attttgtctt aaacggaccg ccgtcatcgc gccaccaggg 3660

.......... .......... .......... .......... rrnB_term ..........

.......... .......... .......... .......... .......... ..........

3661 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc 3720

3661 tggactgggg tacggcttga gtcttcactt tgcggcatcg cggctaccat cacaccccag 3720

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct c agtcgaaag 3780

3721 aggggtacgc tctcatccct tgacggtccg tagtttattt tgctttccga gtcagctttc 3780

.......... .......... rrnB_term ......... rrnB_T1_term .........

.......... .......... .......... .......... .......... ..........

3781 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc 3840

3781 tgacccggaa agcaaaatag acaacaaaca gccacttgcg agaggactca tcctgtttag 3840

rrnB_term rrnB_T1_term ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc 3900

3841 gcggccctcg cctaaacttg caacgcttcg ttgccgggcc tcccaccgcc cgtcctgcgg 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg 3960

3901 gcggtatttg acggtccgta gtttaattcg tcttccggta ggactgccta ccggaaaaac 3960

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

3961 cgtttctaca aactcttttt gtttattttt ctaaatacat tcaaatatgt atccgctcat 4020

3961 gcaaagatgt ttgagaaaaa caaataaaaa gatttatgta agtttataca taggcgagta 4020

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4021 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca 4080

4021 ctctgttatt gggactattt acgaagttat tataactttt tccttctcat actcataagt 4080

.......... .......... .......... .......... ....... ORF 2 Ampicillin

.......... .......... .......... .......... .......... ..........

4081 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca 4140

4081 tgtaaaggca cagcgggaat aagggaaaaa acgccgtaaa acggaaggac aaaaacgagt 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta 4200

4141 gggtctttgc gaccactttc attttctacg acttctagtc aacccacgtg ctcacccaat 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt 4260

4201 gtagcttgac ctagagttgt cgccattcta ggaactctca aaagcggggc ttcttgcaaa 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc 4320

4261 aggttactac tcgtgaaaat ttcaagacga tacaccgcgc cataataggg cacaactgcg 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc 4380

4321 gcccgttctc gttgagccag cggcgtatgt gataagagtc ttactgaacc aactcatgag 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc 4440

4381 tggtcagtgt cttttcgtag aatgcctacc gtactgtcat tctcttaata cgtcacgacg 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa 4500

4441 gtattggtac tcactattgt gacgccggtt gaatgaagac tgttgctagc ctcctggctt 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga 4560

4501 cctcgattgg cgaaaaaacg tgttgtaccc cctagtacat tgagcggaac tagcaaccct 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat 4620

4561 tggcctcgac ttacttcggt atggtttgct gctcgcactg tggtgctacg gacatcgtta 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca 4680

4621 ccgttgttgc aacgcgtttg ataattgacc gcttgatgaa tgagatcgaa gggccgttgt 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

AseI(4682) .......... .......... .......... .......... ..........

4681 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc 4740

4681 taattatctg acctacctcc gcctatttca acgtcctggt gaagacgcga gccgggaagg 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat 4800

4741 ccgaccgacc aaataacgac tatttagacc tcggccactc gcacccagag cgccatagta 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag 4860

4801 acgtcgtgac cccggtctac cattcgggag ggcatagcat caatagatgt gctgcccctc 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa 4920

4861 agtccgttga tacctacttg ctttatctgt ctagcgactc tatccacgga gtgactaatt 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca 4980

4921 cgtaaccatt gacagtctgg ttcaaatgag tatatatgaa atctaactaa attttgaagt 4980

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc 5040

4981 aaaaattaaa ttttcctaga tccacttcta ggaaaaacta ttagagtact ggttttaggg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 5100

5041 aattgcactc aaaagcaagg tgactcgcag tctggggcat cttttctagt ttcctagaag 5100

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

5101 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 5160

5101 aactctagga aaaaaagacg cgcattagac gacgaacgtt tgtttttttg gtggcgatgg 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 5220

5161 tcgccaccaa acaaacggcc tagttctcga tggttgagaa aaaggcttcc attgaccgaa 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt 5280

5221 gtcgtctcgc gtctatggtt tatgacagga agatcacatc ggcatcaatc cggtggtgaa 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 5340

5281 gttcttgaga catcgtggcg gatgtatgga gcgagacgat taggacaatg gtcaccgacg 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 5400

5341 acggtcaccg ctattcagca cagaatggcc caacctgagt tctgctatca atggcctatt 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 5460

5401 ccgcgtcgcc agcccgactt gccccccaag cacgtgtgtc gggtcgaacc tcgcttgctg 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 5520

5461 gatgtggctt gactctatgg atgtcgcact cgatactctt tcgcggtgcg aagggcttcc 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 5580

5521 ctctttccgc ctgtccatag gccattcgcc gtcccagcct tgtcctctcg cgtgctccct 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 5640

5581 cgaaggtccc cctttgcgga ccatagaaat atcaggacag cccaaagcgg tggagactga 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 5700

5641 actcgcagct aaaaacacta cgagcagtcc ccccgcctcg gatacctttt tgcggtcgtt 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc 5760

5701 gcgccggaaa aatgccaagg accggaaaac gaccggaaaa cgagtgtaca agaaaggacg 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg 5820

5761 caatagggga ctaagacacc tattggcata atggcggaaa ctcactcgac tatggcgagc 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat 5880

5821 ggcgtcggct tgctggctcg cgtcgctcag tcactcgctc cttcgccttc tcgcggacta 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag 5940

5881 cgccataaaa gaggaatgcg tagacacgcc ataaagtgtg gcgtatacca cgtgagagtc 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac 6000

5941 atgttagacg agactacggc gtatcaattc ggtcatatgt gaggcgatag cgatgcactg 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 6060

6001 acccagtacc gacgcggggc tgtgggcggt tgtgggcgac tgcgcgggac tgcccgaaca 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 6120

6061 gacgagggcc gtaggcgaat gtctgttcga cactggcaga ggccctcgac gtacacagtc 6120

.......... .......... .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

6121 aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga tcaattcgcg cgcgaaggcg 6180

6121 tccaaaagtg gcagtagtgg ctttgcgcgc tccgtcgtct agttaagcgc gcgcttccgc 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 aagcggcatg cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc 6240

6181 ttcgccgtac gtattacacg gacagtttac ctgcttcgtc cctaagacgt ttgggatacg 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tactccgtca agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca 6300

6241 atgaggcagt tcggcagtta acagactaag caatggttaa tactgttgaa ctgccgatgt 6300

.......... .......... .......... .......... AraC ..........

.......... .......... .......... .......... .......... ..........

6301 tcattcactt tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt 6360

6301 agtaagtgaa aaagaagtgt tggccgtgcc ttgagcgagc ccgaccgggg ccacgtaaaa 6360

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg 6420

6361 aatttatggg cgctctttat ctcaactagc agttttggtt gtaacgctgg ctgccaccgc 6420

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ataggcatcc gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc 6480

6421 tatccgtagg cccaccacga gttttcgtcg aagcggaccg actatgcaac caggagcgcg 6480

.......... .......... AraC .......... .......... ..........

AflII(6484) ........ .......... .......... .......... ..........

6481 cagcttaaga cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac 6540

6481 gtcgaattct gcgattaggg attgacgacc gccttttcta cactgtctgc gctgccgctg 6540

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 aagcaaacat gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg 6600

6541 ttcgtttgta cgacacgctg cgaccgctat agttttaacg acagacggtc cactagcgac 6600

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 atgtactgac aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc 6660

6601 tacatgactg ttcggagcgc atgggctaat aggtagccac ctacctcgct gagcaattag 6660

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gcttccatgc gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag 6720

6661 cgaaggtacg cggcgtcatt gttaacgagt tcgtctaaat agcggtcgtc gaggcttatc 6720

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cgcccttccc cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg 6780

6721 gcgggaaggg gaacgggccg caattactaa acgggtttgt ccagcgactt tacgccgacc 6780

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat 6840

6781 acgcgaagta ggcccgcttt cttggggcat aaccgtttat aactgccggt caattcggta 6840

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... NruI(6891)

6841 tcatgccagt aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc 6900

6841 agtacggtca tccgcgcgcc tgctttcatt tgggtgacca ctatggtaag cgctcggagg 6900

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ggatgacgac cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg 6960

6901 cctactgctg gcatcactac ttagagagga ccgcccttgt cgttttatag tgggccagcc 6960

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 caaacaaatt ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga 7020

6961 gtttgtttaa gagcagggac taaaaagtgg tgggggactg gcgcttacca ctctaactct 7020

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 atataacctt tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca 7080

7021 tatattggaa agtaagggtc gccagccagc tattttttta gctctattgg caaccggagt 7080

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 atcggcgtta aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca 7140

7081 tagccgcaat ttgggcggtg gtctacccgt aatttgctca tagggccgtc gtcccctagt 7140

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ttttgcgctt cagccatact tttcatactc ccgccattca gag....... .......... 7183

7141 aaaacgcgaa gtcggtatga aaagtatgag ggcggtaagt ctc....... .......... 7183

AraC .......... .......... .......... .......... ..........