Sequence of pBAD-TOPO

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Sequence of pBAD-TOPO

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Sequence of pBAD-TOPO : 4127 BP; 1038 A; 1063 C; 1019 G; 1007 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... .......... .......... .......... NcoI(344) ..........

301 ctagaaataa ttttgtttaa ctttaagaag gagatataca tacccatggg ctctggatcc 360

301 gatctttatt aaaacaaatt gaaattcttc ctctatatgt atgggtaccc gagacctagg 360

.......... ....... T7_transl_en_RBS ....... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ggtgatgacg atgacaagct cgcccttaag ggcgagcttg aaggtaagcc tatccctaac 420

361 ccactactgc tactgttcga gcgggaattc ccgctcgaac ttccattcgg atagggattg 420

.......... EK .......... .......... .......... V5

.......... .......... .......... .......... .......... ..........

421 cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttaa 480

421 ggagaggagc cagagctaag atgcgcatgg ccagtagtag tggtagtggt aactcaaatt 480

.......... V5 .......... .......... 6xHis ..........

.......... .......... .......... .......... .......... ..........

481 acggtctcca gcttggctgt tttggcggat gagagaagat tttcagcctg atacagatta 540

481 tgccagaggt cgaaccgaca aaaccgccta ctctcttcta aaagtcggac tatgtctaat 540

.......... .......... .......... .......... . pBAD_rev_pri pTrcHis_rev_pri

.......... .......... .......... .......... .......... ..........

541 aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt agcgcggtgg 600

541 ttagtcttgc gtcttcgcca gactattttg tcttaaacgg accgccgtca tcgcgccacc 600

.......... .......... .......... .......... rrnB_term ..........

.......... .......... .......... .......... .......... ..........

601 tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg 660

601 agggtggact ggggtacggc ttgagtcttc actttgcggc atcgcggcta ccatcacacc 660

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa gg ctcagtcg 720

661 ccagaggggt acgctctcat cccttgacgg tccgtagttt attttgcttt ccgagtcagc 720

.......... .......... rrnB_term ......... rrnB_T1_term .........

.......... .......... .......... .......... .......... ..........

721 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca 780

721 tttctgaccc ggaaagcaaa atagacaaca aacagccact tgcgagagga ctcatcctgt 780

rrnB_term rrnB_T1_term ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga 840

781 ttaggcggcc ctcgcctaaa cttgcaacgc ttcgttgccg ggcctcccac cgcccgtcct 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt 900

841 gcgggcggta tttgacggtc cgtagtttaa ttcgtcttcc ggtaggactg cctaccggaa 900

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

901 tttgcgtttc tacaaactct ttttgtttat ttttctaaat acattcaaat atgtatccgc 960

901 aaacgcaaag atgtttgaga aaaacaaata aaaagattta tgtaagttta tacataggcg 960

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

961 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 1020

961 agtactctgt tattgggact atttacgaag ttattataac tttttccttc tcatactcat 1020

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 1080

1021 aagttgtaaa ggcacagcgg gaataaggga aaaaacgccg taaaacggaa ggacaaaaac 1080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 1140

1081 gagtgggtct ttgcgaccac tttcattttc tacgacttct agtcaaccca cgtgctcacc 1140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 1200

1141 caatgtagct tgacctagag ttgtcgccat tctaggaact ctcaaaagcg gggcttcttg 1200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 1260

1201 caaaaggtta ctactcgtga aaatttcaag acgatacacc gcgccataat agggcacaac 1260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 1320

1261 tgcggcccgt tctcgttgag ccagcggcgt atgtgataag agtcttactg aaccaactca 1320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 1380

1321 tgagtggtca gtgtcttttc gtagaatgcc taccgtactg tcattctctt aatacgtcac 1380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 1440

1381 gacggtattg gtactcacta ttgtgacgcc ggttgaatga agactgttgc tagcctcctg 1440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 1500

1441 gcttcctcga ttggcgaaaa aacgtgttgt accccctagt acattgagcg gaactagcaa 1500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 1560

1501 cccttggcct cgacttactt cggtatggtt tgctgctcgc actgtggtgc tacggacatc 1560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 1620

1561 gttaccgttg ttgcaacgcg tttgataatt gaccgcttga tgaatgagat cgaagggccg 1620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

AseI(1626) .......... .......... .......... .......... ..........

1621 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 1680

1621 ttgttaatta tctgacctac ctccgcctat ttcaacgtcc tggtgaagac gcgagccggg 1680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 1740

1681 aaggccgacc gaccaaataa cgactattta gacctcggcc actcgcaccc agagcgccat 1740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 1800

1741 agtaacgtcg tgaccccggt ctaccattcg ggagggcata gcatcaatag atgtgctgcc 1800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 1860

1801 cctcagtccg ttgataccta cttgctttat ctgtctagcg actctatcca cggagtgact 1860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 1920

1861 aattcgtaac cattgacagt ctggttcaaa tgagtatata tgaaatctaa ctaaattttg 1920

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 1980

1921 aagtaaaaat taaattttcc tagatccact tctaggaaaa actattagag tactggtttt 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 2040

1981 agggaattgc actcaaaagc aaggtgactc gcagtctggg gcatcttttc tagtttccta 2040

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

2041 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 2100

2041 gaagaactct aggaaaaaaa gacgcgcatt agacgacgaa cgtttgtttt tttggtggcg 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 2160

2101 atggtcgcca ccaaacaaac ggcctagttc tcgatggttg agaaaaaggc ttccattgac 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 2220

2161 cgaagtcgtc tcgcgtctat ggtttatgac aggaagatca catcggcatc aatccggtgg 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 2280

2221 tgaagttctt gagacatcgt ggcggatgta tggagcgaga cgattaggac aatggtcacc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 2340

2281 gacgacggtc accgctattc agcacagaat ggcccaacct gagttctgct atcaatggcc 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 2400

2341 tattccgcgt cgccagcccg acttgccccc caagcacgtg tgtcgggtcg aacctcgctt 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 2460

2401 gctggatgtg gcttgactct atggatgtcg cactcgatac tctttcgcgg tgcgaagggc 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 2520

2461 ttccctcttt ccgcctgtcc ataggccatt cgccgtccca gccttgtcct ctcgcgtgct 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 2580

2521 ccctcgaagg tccccctttg cggaccatag aaatatcagg acagcccaaa gcggtggaga 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 2640

2581 ctgaactcgc agctaaaaac actacgagca gtccccccgc ctcggatacc tttttgcggt 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 2700

2641 cgttgcgccg gaaaaatgcc aaggaccgga aaacgaccgg aaaacgagtg tacaagaaag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 2760

2701 gacgcaatag gggactaaga cacctattgg cataatggcg gaaactcact cgactatggc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 2820

2761 gagcggcgtc ggcttgctgg ctcgcgtcgc tcagtcactc gctccttcgc cttctcgcgg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... NdeI(2868) ..........

2821 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc 2880

2821 actacgccat aaaagaggaa tgcgtagaca cgccataaag tgtggcgtat accacgtgag 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg 2940

2881 agtcatgtta gacgagacta cggcgtatca attcggtcat atgtgaggcg atagcgatgc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 3000

2941 actgacccag taccgacgcg gggctgtggg cggttgtggg cgactgcgcg ggactgcccg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 3060

3001 aacagacgag ggccgtaggc gaatgtctgt tcgacactgg cagaggccct cgacgtacac 3060

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

3061 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag cagatcaatt cgcgcgcgaa 3120

3061 agtctccaaa agtggcagta gtggctttgc gcgctccgtc gtctagttaa gcgcgcgctt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggcgaagcgg catgcataat gtgcctgtca aatggacgaa gcagggattc tgcaaaccct 3180

3121 ccgcttcgcc gtacgtatta cacggacagt ttacctgctt cgtccctaag acgtttggga 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atgctactcc gtcaagccgt caattgtctg attcgttacc aattatgaca acttgacggc 3240

3181 tacgatgagg cagttcggca gttaacagac taagcaatgg ttaatactgt tgaactgccg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tacatcattc actttttctt cacaaccggc acggaactcg ctcgggctgg ccccggtgca 3300

3241 atgtagtaag tgaaaaagaa gtgttggccg tgccttgagc gagcccgacc ggggccacgt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttttttaaat acccgcgaga aatagagttg atcgtcaaaa ccaacattgc gaccgacggt 3360

3301 aaaaaattta tgggcgctct ttatctcaac tagcagtttt ggttgtaacg ctggctgcca 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggcgataggc atccgggtgg tgctcaaaag cagcttcgcc tggctgatac gttggtcctc 3420

3361 ccgctatccg taggcccacc acgagttttc gtcgaagcgg accgactatg caaccaggag 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gcgccagctt aagacgctaa tccctaactg ctggcggaaa agatgtgaca gacgcgacgg 3480

3421 cgcggtcgaa ttctgcgatt agggattgac gaccgccttt tctacactgt ctgcgctgcc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRV(3513) ......... ..........

3481 cgacaagcaa acatgctgtg cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc 3540

3481 gctgttcgtt tgtacgacac gctgcgaccg ctatagtttt aacgacagac ggtccactag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gctgatgtac tgacaagcct cgcgtacccg attatccatc ggtggatgga gcgactcgtt 3600

3541 cgactacatg actgttcgga gcgcatgggc taataggtag ccacctacct cgctgagcaa 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aatcgcttcc atgcgccgca gtaacaattg ctcaagcaga tttatcgcca gcagctccga 3660

3601 ttagcgaagg tacgcggcgt cattgttaac gagttcgtct aaatagcggt cgtcgaggct 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 atagcgccct tccccttgcc cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg 3720

3661 tatcgcggga aggggaacgg gccgcaatta ctaaacgggt ttgtccagcg actttacgcc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctggtgcgct tcatccgggc gaaagaaccc cgtattggca aatattgacg gccagttaag 3780

3721 gaccacgcga agtaggcccg ctttcttggg gcataaccgt ttataactgc cggtcaattc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NruI(3835)

3781 ccattcatgc cagtaggcgc gcggacgaaa gtaaacccac tggtgatacc attcgcgagc 3840

3781 ggtaagtacg gtcatccgcg cgcctgcttt catttgggtg accactatgg taagcgctcg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctccggatga cgaccgtagt gatgaatctc tcctggcggg aacagcaaaa tatcacccgg 3900

3841 gaggcctact gctggcatca ctacttagag aggaccgccc ttgtcgtttt atagtgggcc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcggcaaaca aattctcgtc cctgattttt caccaccccc tgaccgcgaa tggtgagatt 3960

3901 agccgtttgt ttaagagcag ggactaaaaa gtggtggggg actggcgctt accactctaa 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gagaatataa cctttcattc ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc 4020

3961 ctcttatatt ggaaagtaag ggtcgccagc cagctatttt tttagctcta ttggcaaccg 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ctcaatcggc gttaaacccg ccaccagatg ggcattaaac gagtatcccg gcagcagggg 4080

4021 gagttagccg caatttgggc ggtggtctac ccgtaatttg ctcatagggc cgtcgtcccc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 atcattttgc gcttcagcca tacttttcat actcccgcca ttcagag... .......... 4127

4081 tagtaaaacg cgaagtcggt atgaaaagta tgagggcggt aagtctc... .......... 4127

.......... .......... .......... .......... .......... ..........