HH!LOCUS pBAD102 Directional TOPO 4471 bp DNA SYN 12-Sep-2007 DEFINITION pBAD102 Directional TOPO ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4471 /organism="pBAD102 Directional TOPO" /mol_type="other DNA" CDS complement(3567..25) /label="ORF frame 3" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 4..19 /label="araO2" misc_feature 161..172 /label="araO1" misc_feature 203..216 /label="CAP_BS" misc_feature 208..227 /label="pBAD_fwd_primer" misc_feature 213..251 /label="AraI1I2" promoter 248..276 /label="ARA_promoter" misc_feature 317..333 /label="T7_transl_en_RBS" misc_feature 346..674 /label="His_patch_thio" misc_feature 691..705 /label="EK" misc_feature 748..789 /label="V5" misc_feature 799..816 /label="6xHis" misc_feature complement(872..889) /label="pBAD_rev_primer" misc_feature complement(872..889) /label="pTrcHis_rev_primer" terminator 922..1079 /label="rrnB_terminator" terminator 1045..1088 /label="rrnB_T1_terminator" terminator 1220..1247 /label="rrnB_T2_terminator" promoter 1288..1316 /label="AmpR_promoter" CDS 1358..2218 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 1358..2218 /label="Ampicillin" /gene="Ampicillin" rep_origin 2373..2992 /label="pBR322_origin" misc_feature 3389..3411 /label="pGEX_3_primer" misc_feature complement(3567..4445) /label="AraC" ORIGIN 1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT 61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA 121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG 181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG 241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG 301 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TACCCATGGG ATCTGATAAA 361 ATTATTCATC TGACTGATGA TTCTTTTGAT ACTGATGTAC TTAAGGCAGA TGGTGCAATC 421 CTGGTTGATT TCTGGGCACA CTGGTGCGGT CCGTGCAAAA TGATCGCTCC GATTCTGGAT 481 GAAATCGCTG ACGAATATCA GGGCAAACTG ACCGTTGCAA AACTGAACAT CGATCACAAC 541 CCGGGCACTG CGCCGAAATA TGGCATCCGT GGTATCCCGA CTCTGCTGCT GTTCAAAAAC 601 GGTGAAGTGG CGGCAACCAA AGTGGGTGCA CTGTCTAAAG GTCAGTTGAA AGAGTTCCTC 661 GACGCTAACC TGGCCGGCTC TGGATCCGGT GATGACGATG ACAAGCTGGG AATTGATCCC 721 TTCACCAAGG GCGAGCTCAA GCTTGAAGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 781 GATTCTACGC GTACCGGTCA TCATCACCAT CACCATTGAG TTTAAACGGT CTCCAGCTTG 841 GCTGTTTTGG CGGATGAGAG AAGATTTTCA GCCTGATACA GATTAAATCA GAACGCAGAA 901 GCGGTCTGAT AAAACAGAAT TTGCCTGGCG GCAGTAGCGC GGTGGTCCCA CCTGACCCCA 961 TGCCGAACTC AGAAGTGAAA CGCCGTAGCG CCGATGGTAG TGTGGGGTCT CCCCATGCGA 1021 GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT 1081 CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG 1141 GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT 1201 GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC GTTTCTACAA 1261 ACTCTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC 1321 CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT 1381 CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT 1441 GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA 1501 TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG 1561 CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA 1621 ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA 1681 AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG 1741 TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC 1801 TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA 1861 TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT 1921 GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG 1981 GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT 2041 TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG 2101 GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT 2161 GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT 2221 GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA 2281 AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT 2341 TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT 2401 TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG 2461 TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA 2521 GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT 2581 AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA 2641 TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC 2701 GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT 2761 GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA 2821 CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG 2881 AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT 2941 TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT 3001 ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA 3061 TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC 3121 GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT 3181 CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC 3241 TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT 3301 GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA 3361 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG 3421 TCATCACCGA AACGCGCGAG GCAGCAGATC AATTCGCGCG CGAAGGCGAA GCGGCATGCA 3481 TAATGTGCCT GTCAAATGGA CGAAGCAGGG ATTCTGCAAA CCCTATGCTA CTCCGTCAAG 3541 CCGTCAATTG TCTGATTCGT TACCAATTAT GACAACTTGA CGGCTACATC ATTCACTTTT 3601 TCTTCACAAC CGGCACGGAA CTCGCTCGGG CTGGCCCCGG TGCATTTTTT AAATACCCGC 3661 GAGAAATAGA GTTGATCGTC AAAACCAACA TTGCGACCGA CGGTGGCGAT AGGCATCCGG 3721 GTGGTGCTCA AAAGCAGCTT CGCCTGGCTG ATACGTTGGT CCTCGCGCCA GCTTAAGACG 3781 CTAATCCCTA ACTGCTGGCG GAAAAGATGT GACAGACGCG ACGGCGACAA GCAAACATGC 3841 TGTGCGACGC TGGCGATATC AAAATTGCTG TCTGCCAGGT GATCGCTGAT GTACTGACAA 3901 GCCTCGCGTA CCCGATTATC CATCGGTGGA TGGAGCGACT CGTTAATCGC TTCCATGCGC 3961 CGCAGTAACA ATTGCTCAAG CAGATTTATC GCCAGCAGCT CCGAATAGCG CCCTTCCCCT 4021 TGCCCGGCGT TAATGATTTG CCCAAACAGG TCGCTGAAAT GCGGCTGGTG CGCTTCATCC 4081 GGGCGAAAGA ACCCCGTATT GGCAAATATT GACGGCCAGT TAAGCCATTC ATGCCAGTAG 4141 GCGCGCGGAC GAAAGTAAAC CCACTGGTGA TACCATTCGC GAGCCTCCGG ATGACGACCG 4201 TAGTGATGAA TCTCTCCTGG CGGGAACAGC AAAATATCAC CCGGTCGGCA AACAAATTCT 4261 CGTCCCTGAT TTTTCACCAC CCCCTGACCG CGAATGGTGA GATTGAGAAT ATAACCTTTC 4321 ATTCCCAGCG GTCGGTCGAT AAAAAAATCG AGATAACCGT TGGCCTCAAT CGGCGTTAAA 4381 CCCGCCACCA GATGGGCATT AAACGAGTAT CCCGGCAGCA GGGGATCATT TTGCGCTTCA 4441 GCCATACTTT TCATACTCCC GCCATTCAGA G // 0pBAD102 Directional TOPO "Times New Roman HC0 ORF 3"Times New RomanHCɆ0 AraC"Times New RomanHC0araO2"Times New RomanHCɆ0araO1"Times New RomanH0CAP_BS"Times New RomanHC0 pBAD_fwd_pri"Times New RomanHC0AraI1I2"Times New Roman*HCɆ0ARA_pro"Times New RomanH0=MT7_transl_en_RBS"Times New RomanHC0ZHis_patch_thio"Times New RomanHC0EK"Times New RomanHCɆ0V5"Times New RomanH006xHis"Times New RomanHC0hy pBAD_rev_pri"Times New RomanHC0hypTrcHis_rev_pri"Times New RomanHCɆ07 rrnB_term"Times New RomanH0@ rrnB_T1_term"Times New RomanHC0 rrnB_T2_term"Times New RomanHC0$AmpR_pro"Times New RomanHCɆ0NORF 2"Times New Roman(H0N Ampicillin"Times New RomanHC0E  pBR322_origin"Times New RomanHC0= S  pGEX_3_pri"Times New Roman HNcoI"Times New RomanX0bQ?05? HClaI(530),SmaI(542)"Times New Roman06DwQhQJh HSacI(737),HindIII(739)"Times New Roman0ar HFspI(1922),AseI(1970)"Times New Roman0!!T? HNdeI"Times New Roman 0NPRNKY HEcoRV"Times New Roman0}n_n HNruI"Times New RomanS0OTHAA."`. Htest"Times New Roman0So3x Htest"Times New Roman0zqaa"x