Sequence of pBAD102_Directional

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Sequence of pBAD102_Directional_TOPO

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Sequence of pBAD102 Directional TOPO : 4471 BP; 1130 A; 1142 C; 1107 G; 1092 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... .......... .......... .......... NcoI(344) ..........

301 ctagaaataa ttttgtttaa ctttaagaag gagatataca tacccatggg atctgataaa 360

301 gatctttatt aaaacaaatt gaaattcttc ctctatatgt atgggtaccc tagactattt 360

.......... ....... T7_transl_en_RBS ....... ........ His_patch_thio

.......... .......... .......... .......... .......... ..........

361 attattcatc tgactgatga ttcttttgat actgatgtac ttaaggcaga tggtgcaatc 420

361 taataagtag actgactact aagaaaacta tgactacatg aattccgtct accacgttag 420

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctggttgatt tctgggcaca ctggtgcggt ccgtgcaaaa tgatcgctcc gattctggat 480

421 gaccaactaa agacccgtgt gaccacgcca ggcacgtttt actagcgagg ctaagaccta 480

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcacaac 540

481 ctttagcgac tgcttatagt cccgtttgac tggcaacgtt ttgacttgta gctagtgttg 540

.......... ........ His_patch_thio ........ .......... ..........

SmaI(542) .......... .......... .......... .......... ..........

541 ccgggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac 600

541 ggcccgtgac gcggctttat accgtaggca ccatagggct gagacgacga caagtttttg 600

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc 660

601 ccacttcacc gccgttggtt tcacccacgt gacagatttc cagtcaactt tctcaaggag 660

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 gacgctaacc tggccggctc tggatccggt gatgacgatg acaagctggg aattgatccc 720

661 ctgcgattgg accggccgag acctaggcca ctactgctac tgttcgaccc ttaactaggg 720

His_patch_thio ...... .......... EK .......... ..........

.... SacI(737),HindIII(739) .... .......... .......... ..........

721 ttcaccaagg gcgagctcaa gcttgaaggt aagcctatcc ctaaccctct cctcggtctc 780

721 aagtggttcc cgctcgagtt cgaacttcca ttcggatagg gattgggaga ggagccagag 780

.......... .......... .......... .......... V5 ..........

.......... .......... .......... .......... .......... ..........

781 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacggt ctccagcttg 840

781 ctaagatgcg catggccagt agtagtggta gtggtaactc aaatttgcca gaggtcgaac 840

V5 .......... 6xHis .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa 900

841 cgacaaaacc gcctactctc ttctaaaagt cggactatgt ctaatttagt cttgcgtctt 900

.......... .......... . pBAD_rev_pri pTrcHis_rev_pri . ..........

.......... .......... .......... .......... .......... ..........

901 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca 960

901 cgccagacta ttttgtctta aacggaccgc cgtcatcgcg ccaccagggt ggactggggt 960

.......... .......... .......... .......... rrnB_term ..........

.......... .......... .......... .......... .......... ..........

961 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga 1020

961 acggcttgag tcttcacttt gcggcatcgc ggctaccatc acaccccaga ggggtacgct 1020

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1080

1021 ctcatccctt gacggtccgt agtttatttt gctttccgag tcagctttct gacccggaaa 1080

.......... .......... rrnB_term ......... rrnB_T1_term .........

.......... .......... .......... .......... .......... ..........

1081 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg 1140

1081 gcaaaataga caacaaacag ccacttgcga gaggactcat cctgtttagg cggccctcgc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact 1200

1141 ctaaacttgc aacgcttcgt tgccgggcct cccaccgccc gtcctgcggg cggtatttga 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa 1260

1201 cggtccgtag tttaattcgt cttccggtag gactgcctac cggaaaaacg caaagatgtt 1260

.......... .......... ......... rrnB_T2_term ......... ..........

.......... .......... .......... .......... .......... ..........

1261 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 1320

1261 tgagaaaaca aataaaaaga tttatgtaag tttatacata ggcgagtact ctgttattgg 1320

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

1321 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 1380

1321 gactatttac gaagttatta taactttttc cttctcatac tcataagttg taaaggcaca 1380

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

1381 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 1440

1381 gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa aaacgagtgg gtctttgcga 1440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 1500

1441 ccactttcat tttctacgac ttctagtcaa cccacgtgct cacccaatgt agcttgacct 1500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 1560

1501 agagttgtcg ccattctagg aactctcaaa agcggggctt cttgcaaaag gttactactc 1560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca 1620

1561 gtgaaaattt caagacgata caccgcgcca taatagggca caactgcggc ccgttctcgt 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 1680

1621 tgagccagcg gcgtatgtga taagagtctt actgaaccaa ctcatgagtg gtcagtgtct 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 1740

1681 tttcgtagaa tgcctaccgt actgtcattc tcttaatacg tcacgacggt attggtactc 1740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 1800

1741 actattgtga cgccggttga atgaagactg ttgctagcct cctggcttcc tcgattggcg 1800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 1860

1801 aaaaaacgtg ttgtaccccc tagtacattg agcggaacta gcaacccttg gcctcgactt 1860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 1920

1861 acttcggtat ggtttgctgc tcgcactgtg gtgctacgga catcgttacc gttgttgcaa 1920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

FspI(1922) .......... .......... .......... AseI(1970) ..........

1921 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 1980

1921 cgcgtttgat aattgaccgc ttgatgaatg agatcgaagg gccgttgtta attatctgac 1980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 2040

1981 ctacctccgc ctatttcaac gtcctggtga agacgcgagc cgggaaggcc gaccgaccaa 2040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 2100

2041 ataacgacta tttagacctc ggccactcgc acccagagcg ccatagtaac gtcgtgaccc 2100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 2160

2101 cggtctacca ttcgggaggg catagcatca atagatgtgc tgcccctcag tccgttgata 2160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 2220

2161 cctacttgct ttatctgtct agcgactcta tccacggagt gactaattcg taaccattga 2220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 2280

2221 cagtctggtt caaatgagta tatatgaaat ctaactaaat tttgaagtaa aaattaaatt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 2340

2281 ttcctagatc cacttctagg aaaaactatt agagtactgg ttttagggaa ttgcactcaa 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 2400

2341 aagcaaggtg actcgcagtc tggggcatct tttctagttt cctagaagaa ctctaggaaa 2400

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 2460

2401 aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc gccaccaaac 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 2520

2461 aaacggccta gttctcgatg gttgagaaaa aggcttccat tgaccgaagt cgtctcgcgt 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 2580

2521 ctatggttta tgacaggaag atcacatcgg catcaatccg gtggtgaagt tcttgagaca 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 2640

2581 tcgtggcgga tgtatggagc gagacgatta ggacaatggt caccgacgac ggtcaccgct 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 2700

2641 attcagcaca gaatggccca acctgagttc tgctatcaat ggcctattcc gcgtcgccag 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 2760

2701 cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga tgtggcttga 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 2820

2761 ctctatggat gtcgcactcg atactctttc gcggtgcgaa gggcttccct ctttccgcct 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 2880

2821 gtccataggc cattcgccgt cccagccttg tcctctcgcg tgctccctcg aaggtccccc 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 2940

2881 tttgcggacc atagaaatat caggacagcc caaagcggtg gagactgaac tcgcagctaa 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 3000

2941 aaacactacg agcagtcccc ccgcctcgga tacctttttg cggtcgttgc gccggaaaaa 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 3060

3001 tgccaaggac cggaaaacga ccggaaaacg agtgtacaag aaaggacgca ataggggact 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 3120

3061 aagacaccta ttggcataat ggcggaaact cactcgacta tggcgagcgg cgtcggcttg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct 3180

3121 ctggctcgcg tcgctcagtc actcgctcct tcgccttctc gcggactacg ccataaaaga 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NdeI(3212) .......... ..........

3181 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc 3240

3181 ggaatgcgta gacacgccat aaagtgtggc gtataccacg tgagagtcat gttagacgag 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct 3300

3241 actacggcgt atcaattcgg tcatatgtga ggcgatagcg atgcactgac ccagtaccga 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 3360

3301 cgcggggctg tgggcggttg tgggcgactg cgcgggactg cccgaacaga cgagggccgt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 3420

3361 aggcgaatgt ctgttcgaca ctggcagagg ccctcgacgt acacagtctc caaaagtggc 3420

.......... .......... .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca 3480

3421 agtagtggct ttgcgcgctc cgtcgtctag ttaagcgcgc gcttccgctt cgccgtacgt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag 3540

3481 attacacgga cagtttacct gcttcgtccc taagacgttt gggatacgat gaggcagttc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc attcactttt 3600

3541 ggcagttaac agactaagca atggttaata ctgttgaact gccgatgtag taagtgaaaa 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc 3660

3601 agaagtgttg gccgtgcctt gagcgagccc gaccggggcc acgtaaaaaa tttatgggcg 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg 3720

3661 ctctttatct caactagcag ttttggttgt aacgctggct gccaccgcta tccgtaggcc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg 3780

3721 caccacgagt tttcgtcgaa gcggaccgac tatgcaacca ggagcgcggt cgaattctgc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc 3840

3781 gattagggat tgacgaccgc cttttctaca ctgtctgcgc tgccgctgtt cgtttgtacg 3840

.......... .......... .......... .......... .......... ..........

......... EcoRV(3857) ......... .......... .......... ..........

3841 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa 3900

3841 acacgctgcg accgctatag ttttaacgac agacggtcca ctagcgacta catgactgtt 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc 3960

3901 cggagcgcat gggctaatag gtagccacct acctcgctga gcaattagcg aaggtacgcg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct 4020

3961 gcgtcattgt taacgagttc gtctaaatag cggtcgtcga ggcttatcgc gggaagggga 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc 4080

4021 acgggccgca attactaaac gggtttgtcc agcgacttta cgccgaccac gcgaagtagg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag 4140

4081 cccgctttct tggggcataa ccgtttataa ctgccggtca attcggtaag tacggtcatc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NruI(4179) .......... ..........

4141 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg 4200

4141 cgcgcgcctg ctttcatttg ggtgaccact atggtaagcg ctcggaggcc tactgctggc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct 4260

4201 atcactactt agagaggacc gcccttgtcg ttttatagtg ggccagccgt ttgtttaaga 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc 4320

4261 gcagggacta aaaagtggtg ggggactggc gcttaccact ctaactctta tattggaaag 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa 4380

4321 taagggtcgc cagccagcta tttttttagc tctattggca accggagtta gccgcaattt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca 4440

4381 gggcggtggt ctacccgtaa tttgctcata gggccgtcgt cccctagtaa aacgcgaagt 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gccatacttt tcatactccc gccattcaga g......... .......... .......... 4471

4441 cggtatgaaa agtatgaggg cggtaagtct c......... .......... .......... 4471

.......... .......... .......... .......... .......... ..........