J~J~)LOCUS pBAD18-Cm 6026 bp DNA SYN 12-Sep-2007 DEFINITION pBAD18-Cm ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6026 /organism="pBAD18-Cm" /mol_type="other DNA" misc_feature complement(96..974) /label="AraC" CDS complement(96..1025) /label="ORF frame 3" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 1004..1019 /label="araO2" misc_feature 1161..1172 /label="araO1" misc_feature 1203..1216 /label="CAP_BS" misc_feature 1208..1227 /label="pBAD_fwd_primer" misc_feature 1213..1251 /label="AraI1I2" promoter 1248..1276 /label="ARA_promoter" misc_feature complement(1395..1412) /label="pBAD_rev_primer" misc_feature complement(1395..1412) /label="pTrcHis_rev_primer" terminator 1445..1602 /label="rrnB_terminator" terminator 1568..1611 /label="rrnB_T1_terminator" terminator 1743..1770 /label="rrnB_T2_terminator" promoter 1811..1839 /label="AmpR_promoter" CDS 1881..2510 /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRAAEVARNREWNQPVIPRYYD*" CDS complement(3106..3765) /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene complement(3106..3765) /label="CAT/CamR" /gene="CAT/CamR" rep_origin 4213..4512 /label="f1_origin" rep_origin 4746..5365 /label="pBR322_origin" misc_feature 5762..5784 /label="pGEX_3_primer" misc_feature 5923..5942 /label="pBRrevBam_primer" ORIGIN 1 ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC 61 TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA 121 TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA 181 AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA 241 GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG 301 CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG 361 CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG 421 TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT 481 TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC 541 CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC 601 GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA 661 TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA 721 TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA 781 ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA 841 TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC 901 GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT 961 TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT 1021 TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA 1081 ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT 1141 AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA 1201 CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG ATCCTACCTG ACGCTTTTTA 1261 TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCGAATT CGAGCTCGGT 1321 ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCTGTTT TGGCGGATGA 1381 GAGAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG 1441 AATTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG 1501 AAACGCCGTA GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG 1561 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT 1621 GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA 1681 GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAATTAA 1741 GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT 1801 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 1861 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 1921 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 1981 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 2041 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 2101 TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 2161 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 2221 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 2281 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 2341 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 2401 ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGTGCT GCTGAAGTTG 2461 CCCGCAACAG AGAGTGGAAC CAACCGGTGA TACCACGATA CTATGACTGA GAGTCAACGC 2521 CATGAGCGGC CTCATTTCTT ATTCTGAGTT ACAACAGTCC GCACCGCTGT CCGGTAGCTC 2581 CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT ATGACTGTCT TCTTTATCAT 2641 GCAACTCGTA GGACAGGTGC CGGCAGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC 2701 CATACCCACG CCGAAACAAG CGCCCTGCAC CATTATGTTC CGGATCTGCA TCGCAGGATG 2761 CTGCTGGCTA CCCTGTGGAA CACCTACATC TGTATTAACG AAGCGCTAAC CGTTTTTATC 2821 AGGCTCTGGG AGGCAGAATA AATGATCATA TCGTCAATTA TTACCTCCAC GGGGAGAGCC 2881 TGAGCAAACT GGCCTCAGGC ATTTGAGAAG CACACGGTCA CACTGCTTCC GGTAGTCAAT 2941 AAACCGGTAA ACCAGCAATA GACATAAGCG GCTATTTAAC GACCCTGCCC TGAACCGACG 3001 ACCGGGTCGA ATTTGCTTTC GAATTTCTGC CATTCATCCG CTTATTATCA CTTATTCAGG 3061 CGTAGCACCA GGCGTTTAAG GGCACCAATA ACTGCCTTAA AAAAATTACG CCCCGCCCTG 3121 CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA AGCCATCACA 3181 GACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT GCGTATAATA 3241 TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT TTAAATCAAA 3301 ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA TAAACCCTTT 3361 AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA TGTGTAGAAA 3421 CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG TTTGCTCATG 3481 GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT CTTTCATTGC 3541 CATACGGAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA AGGCCGGATA 3601 AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA GCTGAACGGT 3661 CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT TACGATGCCA 3721 TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG CTTCCTTAGC 3781 TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT CATTATGGTG 3841 AAAGTTGGAA CCTCTTACGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC 3901 AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC 3961 TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA 4021 TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG 4081 GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA 4141 TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTACGCG 4201 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 4261 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 4321 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 4381 TTACGGCACC TCGACCCCAA AAAACTTGAT TTGGGTGATG GTTCACGTAG TGGGCCATCG 4441 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 4501 TTGTTCCAAA CTTGAACAAC ACTCAACCCT ATCTCGGGCT ATTCTTTTGA TTTATAAGGG 4561 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 4621 AATTTTAACA AAATATTAAC GTTTACAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA 4681 TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT 4741 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA 4801 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT 4861 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA 4921 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT 4981 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC 5041 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA 5101 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA 5161 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG 5221 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT 5281 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG 5341 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT 5401 TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT 5461 TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA 5521 GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA 5581 CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC 5641 ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT 5701 GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC 5761 TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAA 5821 GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA 5881 AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA 5941 GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG 6001 GATCTGCTCA TGTTTGACAG CTTATC //  pBAD18-Cm "Times New Roman!J~C`AraC"Times New RomanJ~C`ORF 3"Times New RomanJ~CɆaraO2"Times New RomanJ~araO1"Times New RomanJ~CCAP_BS"Times New RomanJ~C pBAD_fwd_pri"Times New RomanJ~CɆAraI1I2"Times New Roman*J~ARA_pro"Times New RomanJ~Cs pBAD_rev_pri"Times New RomanJ~CspTrcHis_rev_pri"Times New RomanJ~CɆB rrnB_term"Times New RomanJ~ K rrnB_T1_term"Times New RomanJ~C rrnB_T2_term"Times New RomanJ~C/AmpR_pro"Times New RomanJ~CɆY ORF 3"Times New RomanJ~" ORF 1"Times New RomanJ~C" CAT/CamR"Times New RomanJ~Cu f1_origin"Times New RomanJ~CɆ pBR322_origin"Times New RomanJ~ pGEX_3_pri"Times New RomanJ~C#6 pBRrevBam_pri"Times New Roman J~ClaI"Times New RomanJ6"" J~AflII(301),EcoRV(386)"Times New Roman-T@050 J~NruI"Times New Roman1?vLgLXg J~ONheI(1300),SacI(1316),KpnI(1322),SmaI(1324),XbaI(1333),SalI(1339),HindIII(1357)"Times New Romanx& J~BclI"Times New Roman %4-; J~BstBI"Times New Roman *>v>M J~NcoI(3246),MscI(3284)"Times New Roman d_#N3 3NQ J~PvuII"Times New RomanC  J~AseI"Times New RomanB J~NdeI"Times New Roman/c&Q?0? J~test"Times New Roman Qs]HHZ J~test"Times New Roman6}@ANN9