Sequence of pBAD18-Cm

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Sequence of pBAD18-Cm

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Sequence of pBAD18-Cm : 6026 BP; 1504 A; 1576 C; 1465 G; 1481 T; 0 other;

ClaI(2) .......... .......... .......... .......... ..........

1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 60

1 tagctacgta ttacacggac agtttacctg cttcgtccct aagacgtttg ggatacgatg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 120

61 aggcagttcg gcagttaaca gactaagcaa tggttaatac tgttgaactg ccgatgtagt 120

.......... .......... .......... .......... AraC ORF 3 ..........

.......... .......... .......... .......... .......... ..........

121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 180

121 aagtgaaaaa gaagtgttgg ccgtgccttg agcgagcccg accggggcca cgtaaaaaat 180

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 240

181 ttatgggcgc tctttatctc aactagcagt tttggttgta acgctggctg ccaccgctat 240

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 300

241 ccgtaggccc accacgagtt ttcgtcgaag cggaccgact atgcaaccag gagcgcggtc 300

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 360

301 gaattctgcg attagggatt gacgaccgcc ttttctacac tgtctgcgct gccgctgttc 360

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... EcoRV(386) .......... .......... ..........

361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 420

361 gtttgtacga cacgctgcga ccgctatagt tttaacgaca gacggtccac tagcgactac 420

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 480

421 atgactgttc ggagcgcatg ggctaatagg tagccaccta cctcgctgag caattagcga 480

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 540

481 aggtacgcgg cgtcattgtt aacgagttcg tctaaatagc ggtcgtcgag gcttatcgcg 540

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 600

541 ggaaggggaa cgggccgcaa ttactaaacg ggtttgtcca gcgactttac gccgaccacg 600

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 660

601 cgaagtaggc ccgctttctt ggggcataac cgtttataac tgccggtcaa ttcggtaagt 660

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... NruI(708) ..........

661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 720

661 acggtcatcc gcgcgcctgc tttcatttgg gtgaccacta tggtaagcgc tcggaggcct 720

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 780

721 actgctggca tcactactta gagaggaccg cccttgtcgt tttatagtgg gccagccgtt 780

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 840

781 tgtttaagag cagggactaa aaagtggtgg gggactggcg cttaccactc taactcttat 840

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 900

841 attggaaagt aagggtcgcc agccagctat ttttttagct ctattggcaa ccggagttag 900

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 960

901 ccgcaatttg ggcggtggtc tacccgtaat ttgctcatag ggccgtcgtc ccctagtaaa 960

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 1020

961 acgcgaagtc ggtatgaaaa gtatgagggc ggtaagtctc ttctttggtt aacaggtata 1020

AraC .......... ORF 3 .......... .......... araO2

.......... .......... .......... .......... .......... ..........

1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 1080

1021 acgtagtctg taacggcagt gacgcagaaa atgaccgaga agagcgattg gtttggccat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 1140

1081 tggggcgaat aattttcgta agacattgtt tcgccctggt ttcggtactg tttttgcgca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 1200

1141 ttgttttcac agatattagt gccgtctttt caggtgtaac taataaacgt gccgcagtgt 1200

.......... .......... araO1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 1260

1201 gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc taggatggac tgcgaaaaat 1260

CAP_BS pBAD_fwd_pri ........ AraI1I2 .......... ARA_pro

.......... .......... .......... NheI(1300) .......... ..........

1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt 1320

1261 agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc gatcgcttaa gctcgagcca 1320

ARA_pro .......... .......... .......... .......... ..........

KpnI,SmaI324) XbaI(1333) SalI(1339) HindIII(1357) ..

1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga 1380

1321 tgggccccta ggagatctca gctggacgtc cgtacgttcg aaccgacaaa accgcctact 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag 1440

1381 ctcttctaaa agtcggacta tgtctaattt agtcttgcgt cttcgccaga ctattttgtc 1440

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg 1500

1441 ttaaacggac cgccgtcatc gcgccaccag ggtggactgg ggtacggctt gagtcttcac 1500

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag 1560

1501 tttgcggcat cgcggctacc atcacacccc agaggggtac gctctcatcc cttgacggtc 1560

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1620

1561 cgtagtttat tttgctttcc gagtcagctt tctgacccgg aaagcaaaat agacaacaaa 1620

.......... .... rrnB_term rrnB_T1_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa 1680

1621 cagccacttg cgagaggact catcctgttt aggcggccct cgcctaaact tgcaacgctt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa 1740

1681 cgttgccggg cctcccaccg cccgtcctgc gggcggtatt tgacggtccg tagtttaatt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt 1800

1741 cgtcttccgg taggactgcc taccggaaaa acgcaaagat gtttgagaaa acaaataaaa 1800

......... rrnB_T2_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1860

1801 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1860

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1920

1861 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1920

.......... .......... .......... ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1980

1921 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1980

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 2040

1981 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 2040

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2100

2041 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2100

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 2160

2101 atacaccgcg ccataatagg gcacaactgc ggcccgttct cgttgagcca gcggcgtatg 2160

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2220

2161 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2220

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2280

2221 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2340

2281 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2400

2341 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2400

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgtgct gctgaagttg 2460

2401 tgctcgcact gtggtgctac ggacgtcgtt accgttgttg caacgcacga cgacttcaac 2460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cccgcaacag agagtggaac caaccggtga taccacgata ctatgactga gagtcaacgc 2520

2461 gggcgttgtc tctcaccttg gttggccact atggtgctat gatactgact ctcagttgcg 2520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 catgagcggc ctcatttctt attctgagtt acaacagtcc gcaccgctgt ccggtagctc 2580

2521 gtactcgccg gagtaaagaa taagactcaa tgttgtcagg cgtggcgaca ggccatcgag 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat 2640

2581 gaaggccacc cgcgccccgt actgatagca gcggcgtgaa tactgacaga agaaatagta 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcaactcgta ggacaggtgc cggcagcgcc caacagtccc ccggccacgg ggcctgccac 2700

2641 cgttgagcat cctgtccacg gccgtcgcgg gttgtcaggg ggccggtgcc ccggacggtg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catacccacg ccgaaacaag cgccctgcac cattatgttc cggatctgca tcgcaggatg 2760

2701 gtatgggtgc ggctttgttc gcgggacgtg gtaatacaag gcctagacgt agcgtcctac 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctaac cgtttttatc 2820

2761 gacgaccgat gggacacctt gtggatgtag acataattgc ttcgcgattg gcaaaaatag 2820

.......... .......... .......... .......... .......... ..........

.......... .......... BclI(2843) .......... .......... ..........

2821 aggctctggg aggcagaata aatgatcata tcgtcaatta ttacctccac ggggagagcc 2880

2821 tccgagaccc tccgtcttat ttactagtat agcagttaat aatggaggtg cccctctcgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgagcaaact ggcctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat 2940

2881 actcgtttga ccggagtccg taaactcttc gtgtgccagt gtgacgaagg ccatcagtta 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aaaccggtaa accagcaata gacataagcg gctatttaac gaccctgccc tgaaccgacg 3000

2941 tttggccatt tggtcgttat ctgtattcgc cgataaattg ctgggacggg acttggctgc 3000

.......... .......... .......... .......... .......... ..........

......... BstBI(3019) ......... .......... .......... ..........

3001 accgggtcga atttgctttc gaatttctgc cattcatccg cttattatca cttattcagg 3060

3001 tggcccagct taaacgaaag cttaaagacg gtaagtaggc gaataatagt gaataagtcc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgtagcacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg ccccgccctg 3120

3061 gcatcgtggt ccgcaaattc ccgtggttat tgacggaatt tttttaatgc ggggcgggac 3120

.......... .......... .......... .......... ........ ORF 1 CAT/CamR

.......... .......... .......... .......... .......... ..........

3121 ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca 3180

3121 ggtgagtagc gtcatgacaa cattaagtaa ttcgtaagac ggctgtacct tcggtagtgt 3180

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata 3240

3181 ctgccgtact acttggactt agcggtcgcc gtagtcgtgg aacagcggaa cgcatattat 3240

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

NcoI(3246) .......... .......... .......... MscI(3284) ..........

3241 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa 3300

3241 aaacgggtac cacttttgcc cccgcttctt caacaggtat aaccggtgca aatttagttt 3300

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt 3360

3301 tgaccacttt gagtgggtcc ctaaccgact ctgctttttg tataagagtt atttgggaaa 3360

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa 3420

3361 tccctttatc cggtccaaaa gtggcattgt gcggtgtaga acgcttatat acacatcttt 3420

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg 3480

3421 gacggccttt agcagcacca taagtgaggt ctcgctactt ttgcaaagtc aaacgagtac 3480

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc 3540

3481 cttttgccac attgttccca cttgtgatag ggtatagtgg tcgagtggca gaaagtaacg 3540

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata 3600

3541 gtatgcctta aggcctactc gtaagtagtc cgcccgttct tacacttatt tccggcctat 3600

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... ......... PvuII(3651)

3601 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt 3660

3601 tttgaacacg aataaaaaga aatgccagaa atttttccgg cattataggt cgacttgcca 3660

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca 3720

3661 gaccaatatc catgtaactc gttgactgac tttacggagt tttacaagaa atgctacggt 3720

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc 3780

3721 aaccctatat agttgccacc atataggtca ctaaaaaaag aggtaaaatc gaaggaatcg 3780

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg 3840

3781 aggactttta gagctattga gttttttatg cgggccatca ctagaataaa gtaataccac 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aaagttggaa cctcttacgc aaactattaa ctggcgaact acttactcta gcttcccggc 3900

3841 tttcaacctt ggagaatgcg tttgataatt gaccgcttga tgaatgagat cgaagggccg 3900

.......... .......... .......... .......... .......... ..........

AseI(3906) .......... .......... .......... .......... ..........

3901 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 3960

3901 ttgttaatta tctgacctac ctccgcctat ttcaacgtcc tggtgaagac gcgagccggg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 4020

3961 aaggccgacc gaccaaataa cgactattta gacctcggcc actcgcaccc agagcgccat 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 4080

4021 agtaacgtcg tgaccccggt ctaccattcg ggagggcata gcatcaatag atgtgctgcc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 4140

4081 cctcagtccg ttgataccta cttgctttat ctgtctagcg actctatcca cggagtgact 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttacgcg 4200

4141 aattcgtaac cattgacagt ctggttcaaa tgagtatata tgaaatctaa ctaaatgcgc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 4260

4201 gggacatcgc cgcgtaattc gcgccgccca caccaccaat gcgcgtcgca ctggcgatgt 4260

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 4320

4261 gaacggtcgc gggatcgcgg gcgaggaaag cgaaagaagg gaaggaaaga gcggtgcaag 4320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 4380

4321 cggccgaaag gggcagttcg agatttagcc cccgagggaa atcccaaggc taaatcacga 4380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg 4440

4381 aatgccgtgg agctggggtt ttttgaacta aacccactac caagtgcatc acccggtagc 4440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 4500

4441 gggactatct gccaaaaagc gggaaactgc aacctcaggt gcaagaaatt atcacctgag 4500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttgttccaaa cttgaacaac actcaaccct atctcgggct attcttttga tttataaggg 4560

4501 aacaaggttt gaacttgttg tgagttggga tagagcccga taagaaaact aaatattccc 4560

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 4620

4561 taaaacggct aaagccggat aaccaatttt ttactcgact aaattgtttt taaattgcgc 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aattttaaca aaatattaac gtttacaatt taaaaggatc taggtgaaga tcctttttga 4680

4621 ttaaaattgt tttataattg caaatgttaa attttcctag atccacttct aggaaaaact 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 4740

4681 attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca gtctggggca 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 4800

4741 tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga cgacgaacgt 4800

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4801 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 4860

4801 ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg atggttgaga 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 4920

4861 aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacagg aagatcacat 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 4980

4921 cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg agcgagacga 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 5040

4981 ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc ccaacctgag 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 5100

5041 ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa gcacgtgtgt 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 5160

5101 cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac tcgatactct 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 5220

5161 ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc cgtcccagcc 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 5280

5221 ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa tatcaggaca 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 5340

5281 gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc cccccgcctc 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 5400

5341 ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa cgaccggaaa 5400

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 5460

5401 acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat aatggcggaa 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 5520

5461 actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca gtcactcgct 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 5580

5521 ccttcgcctt ctcgcggact acgccataaa agaggaatgc gtagacacgc cataaagtgt 5580

.......... .......... .......... .......... .......... ..........

NdeI(5585) .......... .......... .......... .......... ..........

5581 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac 5640

5581 ggcgtatacc acgtgagagt catgttagac gagactacgg cgtatcaatt cggtcatatg 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct 5700

5641 tgaggcgata gcgatgcact gacccagtac cgacgcgggg ctgtgggcgg ttgtgggcga 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 5760

5701 ctgcgcggga ctgcccgaac agacgagggc cgtaggcgaa tgtctgttcg acactggcag 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcaa 5820

5761 aggccctcga cgtacacagt ctccaaaagt ggcagtagtg gctttgcgcg ctccgtcgtt 5820

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca 5880

5821 cctctaccgc gggttgtcag ggggccggtg ccccggacgg tggtatgggt gcggctttgt 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata 5940

5881 tcgcgagtac tcgggcttca ccgctcgggc tagaaggggt agccactaca gccgctatat 5940

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

5941 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag 6000

5941 ccgcggtcgt tggcgtggac accgcggcca ctacggccgg tgctacgcag gccgcatctc 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gatctgctca tgtttgacag cttatc.... .......... .......... .......... 6026

6001 ctagacgagt acaaactgtc gaatag.... .......... .......... .......... 6026

.......... .......... .......... .......... .......... ..........