Sequence of pBAD18-Kan

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Sequence of pBAD18-Kan

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Sequence of pBAD18-Kan : 5437 BP; 1339 A; 1379 C; 1337 G; 1382 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 60

1 tagctacgta ttacacggac agtttacctg cttcgtccct aagacgtttg ggatacgatg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 120

61 aggcagttcg gcagttaaca gactaagcaa tggttaatac tgttgaactg ccgatgtagt 120

.......... .......... .......... .......... AraC ORF 3 ..........

.......... .......... .......... .......... .......... ..........

121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 180

121 aagtgaaaaa gaagtgttgg ccgtgccttg agcgagcccg accggggcca cgtaaaaaat 180

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 240

181 ttatgggcgc tctttatctc aactagcagt tttggttgta acgctggctg ccaccgctat 240

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 300

241 ccgtaggccc accacgagtt ttcgtcgaag cggaccgact atgcaaccag gagcgcggtc 300

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 360

301 gaattctgcg attagggatt gacgaccgcc ttttctacac tgtctgcgct gccgctgttc 360

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... EcoRV(386) .......... .......... ..........

361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 420

361 gtttgtacga cacgctgcga ccgctatagt tttaacgaca gacggtccac tagcgactac 420

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 480

421 atgactgttc ggagcgcatg ggctaatagg tagccaccta cctcgctgag caattagcga 480

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 540

481 aggtacgcgg cgtcattgtt aacgagttcg tctaaatagc ggtcgtcgag gcttatcgcg 540

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 600

541 ggaaggggaa cgggccgcaa ttactaaacg ggtttgtcca gcgactttac gccgaccacg 600

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 660

601 cgaagtaggc ccgctttctt ggggcataac cgtttataac tgccggtcaa ttcggtaagt 660

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 720

661 acggtcatcc gcgcgcctgc tttcatttgg gtgaccacta tggtaagcgc tcggaggcct 720

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 780

721 actgctggca tcactactta gagaggaccg cccttgtcgt tttatagtgg gccagccgtt 780

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 840

781 tgtttaagag cagggactaa aaagtggtgg gggactggcg cttaccactc taactcttat 840

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 900

841 attggaaagt aagggtcgcc agccagctat ttttttagct ctattggcaa ccggagttag 900

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 960

901 ccgcaatttg ggcggtggtc tacccgtaat ttgctcatag ggccgtcgtc ccctagtaaa 960

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 1020

961 acgcgaagtc ggtatgaaaa gtatgagggc ggtaagtctc ttctttggtt aacaggtata 1020

AraC .......... ORF 3 .......... .......... araO2

.......... .......... .......... .......... .......... AgeI(1074)

1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 1080

1021 acgtagtctg taacggcagt gacgcagaaa atgaccgaga agagcgattg gtttggccat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 1140

1081 tggggcgaat aattttcgta agacattgtt tcgccctggt ttcggtactg tttttgcgca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 1200

1141 ttgttttcac agatattagt gccgtctttt caggtgtaac taataaacgt gccgcagtgt 1200

.......... .......... araO1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 1260

1201 gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc taggatggac tgcgaaaaat 1260

CAP_BS pBAD_fwd_pri ........ AraI1I2 .......... ARA_pro

.......... .......... ......... NheI(1300) EcoRI(1306) .........

1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt 1320

1261 agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc gatcgcttaa gctcgagcca 1320

ARA_pro .......... .......... .......... .......... ..........

KpnI(1322) XbaI(1333) SalI(1339) PstI(1349) ........ ..........

1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga 1380

1321 tgggccccta ggagatctca gctggacgtc cgtacgttcg aaccgacaaa accgcctact 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag 1440

1381 ctcttctaaa agtcggacta tgtctaattt agtcttgcgt cttcgccaga ctattttgtc 1440

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg 1500

1441 ttaaacggac cgccgtcatc gcgccaccag ggtggactgg ggtacggctt gagtcttcac 1500

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag 1560

1501 tttgcggcat cgcggctacc atcacacccc agaggggtac gctctcatcc cttgacggtc 1560

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1620

1561 cgtagtttat tttgctttcc gagtcagctt tctgacccgg aaagcaaaat agacaacaaa 1620

.......... .... rrnB_term rrnB_T1_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa 1680

1621 cagccacttg cgagaggact catcctgttt aggcggccct cgcctaaact tgcaacgctt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa 1740

1681 cgttgccggg cctcccaccg cccgtcctgc gggcggtatt tgacggtccg tagtttaatt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt 1800

1741 cgtcttccgg taggactgcc taccggaaaa acgcaaagat gtttgagaaa acaaataaaa 1800

......... rrnB_T2_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1860

1801 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1860

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1920

1861 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1920

.......... .......... ...... Ampicillin(0-300) ...... ..........

.......... .......... .......... .......... .......... ..........

1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1980

1921 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1980

.......... ...... Ampicillin(0-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 2040

1981 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 2040

.......... ...... Ampicillin(0-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2100

2041 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2100

.......... ...... Ampicillin(0-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 2160

2101 atacaccgcg ccataatagg gcacaactgc ggcccgttct cgttgagcca gcggcgtatg 2160

.......... ...... Ampicillin(0-300) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 actattctca gaatgacttg gttgagtggg ggggggggga aagccacgtt gtgtctcaaa 2220

2161 tgataagagt cttactgaac caactcaccc ccccccccct ttcggtgcaa cacagagttt 2220

Ampicillin(0-300) .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg 2280

2221 tagagactac aatgtaacgt gttctatttt tatatagtag tacttgttat tttgacagac 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... XhoI(2339)

2281 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct 2340

2281 gaatgtattt gtcattatgt tccccacaat actcggtata agttgccctt tgcagaacga 2340

.......... .......... .......... ......... ORF 3 KanR2 .........

.......... .......... .......... .......... .......... ..........

2341 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg 2400

2341 gctccggcgc taatttaagg ttgtacctac gactaaatat acccatattt acccgagcgc 2400

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag 2460

2401 tattacagcc cgttagtcca cgctgttaga tagctaacat acccttcggg ctacgcggtc 2460

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca 2520

2461 tcaacaaaga ctttgtaccg tttccatcgc aacggttact acaatgtcta ctctaccagt 2520

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc 2580

2521 ctgatttgac cgactgcctt aaatacggag aaggctggta gttcgtaaaa taggcatgag 2580

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc caggtattag 2640

2581 gactactacg taccaatgag tggtgacgct aggggccctt ttgtcgtaag gtccataatc 2640

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt 2700

2641 ttcttatagg actaagtcca cttttataac aactacgcga ccgtcacaag gacgcggcca 2700

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc 2760

2701 acgtaagcta aggacaaaca ttaacaggaa aattgtcgct agcgcataaa gcagagcgag 2760

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta 2820

2761 tccgcgttag tgcttactta ttgccaaacc aactacgctc actaaaacta ctgctcgcat 2820

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg 2880

2821 taccgaccgg acaacttgtt cagacctttc tttacgtatt cgaaaacggt aagagtggcc 2880

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... AseI(2940)

2881 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat 2940

2881 taagtcagca gtgagtacca ctaaagagtg aactattgga ataaaaactg ctccccttta 2940

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca 3000

2941 attatccaac ataactacaa cctgctcagc cttagcgtct ggctatggtc ctagaacggt 3000

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat 3060

3001 aggatacctt gacggagcca ctcaaaagag gaagtaatgt ctttgccgaa aaagttttta 3060

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt 3120

3061 taccataact attaggacta tacttattta acgtcaaagt aaactacgag ctactcaaaa 3120

.......... ......... ORF 3 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg 3180

3121 agattagtct taaccaatta accaacattg tgaccgtctc gtaatgcgac tgaactgccc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 acggcggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct 3240

3181 tgccgccgaa acaacttatt tagcttgaaa acgactcaac ttcctagtct agtgcgtaga 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac 3300

3241 agggctgttg cgtctggcaa ggcaccgttt cgttttcaag ttttagtggt tgaccaggtg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat 3360

3301 gatgttgttt cgagagtagt tggcaccgag ggagtgaaag accgacctac taccccgcta 3360

.......... .......... .......... .......... .......... ..........

StuI(3365) .......... .......... .......... .......... ..........

3361 tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctcagc gccccccccc 3420

3361 agtccggacc atactcagtc gttgtggaag aagtgctccg tctggagtcg cggggggggg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ccctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 3480

3421 gggagcgcca tagtaacgtc gtgaccccgg tctaccattc gggagggcat agcatcaata 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 3540

3481 gatgtgctgc ccctcagtcc gttgatacct acttgcttta tctgtctagc gactctatcc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 3600

3541 acggagtgac taattcgtaa ccattgacag tctggttcaa atgagtatat atgaaatcta 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 3660

3601 actaaatgcg cgggacatcg ccgcgtaatt cgcgccgccc acaccaccaa tgcgcgtcgc 3660

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

3661 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 3720

3661 actggcgatg tgaacggtcg cgggatcgcg ggcgaggaaa gcgaaagaag ggaaggaaag 3720

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 3780

3721 agcggtgcaa gcggccgaaa ggggcagttc gagatttagc ccccgaggga aatcccaagg 3780

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta 3840

3781 ctaaatcacg aaatgccgtg gagctggggt tttttgaact aaacccacta ccaagtgcat 3840

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 3900

3841 cacccggtag cgggactatc tgccaaaaag cgggaaactg caacctcagg tgcaagaaat 3900

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 atagtggact cttgttccaa acttgaacaa cactcaaccc tatctcgggc tattcttttg 3960

3901 tatcacctga gaacaaggtt tgaacttgtt gtgagttggg atagagcccg ataagaaaac 3960

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 4020

3961 taaatattcc ctaaaacggc taaagccgga taaccaattt tttactcgac taaattgttt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttaaaaggat ctaggtgaag 4080

4021 ttaaattgcg cttaaaattg ttttataatt gcaaatgtta aattttccta gatccacttc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg 4140

4081 taggaaaaac tattagagta ctggttttag ggaattgcac tcaaaagcaa ggtgactcgc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc 4200

4141 agtctggggc atcttttcta gtttcctaga agaactctag gaaaaaaaga cgcgcattag 4200

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4201 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag 4260

4201 acgacgaacg tttgtttttt tggtggcgat ggtcgccacc aaacaaacgg cctagttctc 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc 4320

4261 gatggttgag aaaaaggctt ccattgaccg aagtcgtctc gcgtctatgg tttatgacag 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac 4380

4321 gaagatcaca tcggcatcaa tccggtggtg aagttcttga gacatcgtgg cggatgtatg 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc 4440

4381 gagcgagacg attaggacaa tggtcaccga cgacggtcac cgctattcag cacagaatgg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt 4500

4441 cccaacctga gttctgctat caatggccta ttccgcgtcg ccagcccgac ttgcccccca 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt 4560

4501 agcacgtgtg tcgggtcgaa cctcgcttgc tggatgtggc ttgactctat ggatgtcgca 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc 4620

4561 ctcgatactc tttcgcggtg cgaagggctt ccctctttcc gcctgtccat aggccattcg 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt 4680

4621 ccgtcccagc cttgtcctct cgcgtgctcc ctcgaaggtc cccctttgcg gaccatagaa 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca 4740

4681 atatcaggac agcccaaagc ggtggagact gaactcgcag ctaaaaacac tacgagcagt 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt 4800

4741 ccccccgcct cggatacctt tttgcggtcg ttgcgccgga aaaatgccaa ggaccggaaa 4800

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt 4860

4801 acgaccggaa aacgagtgta caagaaagga cgcaataggg gactaagaca cctattggca 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag 4920

4861 taatggcgga aactcactcg actatggcga gcggcgtcgg cttgctggct cgcgtcgctc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc 4980

4921 agtcactcgc tccttcgcct tctcgcggac tacgccataa aagaggaatg cgtagacacg 4980

.......... .......... .......... .......... .......... ..........

.......... NdeI(4996) .......... .......... .......... ..........

4981 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta 5040

4981 ccataaagtg tggcgtatac cacgtgagag tcatgttaga cgagactacg gcgtatcaat 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc 5100

5041 tcggtcatat gtgaggcgat agcgatgcac tgacccagta ccgacgcggg gctgtgggcg 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag 5160

5101 gttgtgggcg actgcgcggg actgcccgaa cagacgaggg ccgtaggcga atgtctgttc 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg 5220

5161 gacactggca gaggccctcg acgtacacag tctccaaaag tggcagtagt ggctttgcgc 5220

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgaggcagca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc 5280

5221 gctccgtcgt tcctctaccg cgggttgtca gggggccggt gccccggacg gtggtatggg 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg 5340

5281 tgcggctttg ttcgcgagta ctcgggcttc accgctcggg ctagaagggg tagccactac 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt 5400

5341 agccgctata tccgcggtcg ttggcgtgga caccgcggcc actacggccg gtgctacgca 5400

pBRrevBam_pri ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ccggcgtaga ggatctgctc atgtttgaca gcttatc... .......... .......... 5437

5401 ggccgcatct cctagacgag tacaaactgt cgaatag... .......... .......... 5437

.......... .......... .......... .......... .......... ..........