Sequence of pBAD18s

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Sequence of pBAD18s

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Sequence of pBAD18s : 4613 BP; 1124 A; 1214 C; 1150 G; 1125 T; 0 other;

ClaI(2) .......... .......... .......... .......... ..........

1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 60

1 tagctacgta ttacacggac agtttacctg cttcgtccct aagacgtttg ggatacgatg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 120

61 aggcagttcg gcagttaaca gactaagcaa tggttaatac tgttgaactg ccgatgtagt 120

.......... .......... .......... .......... AraC ORF 3 ..........

.......... .......... .......... .......... .......... ..........

121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 180

121 aagtgaaaaa gaagtgttgg ccgtgccttg agcgagcccg accggggcca cgtaaaaaat 180

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 240

181 ttatgggcgc tctttatctc aactagcagt tttggttgta acgctggctg ccaccgctat 240

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 300

241 ccgtaggccc accacgagtt ttcgtcgaag cggaccgact atgcaaccag gagcgcggtc 300

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 360

301 gaattctgcg attagggatt gacgaccgcc ttttctacac tgtctgcgct gccgctgttc 360

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... EcoRV(386) .......... .......... ..........

361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 420

361 gtttgtacga cacgctgcga ccgctatagt tttaacgaca gacggtccac tagcgactac 420

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 480

421 atgactgttc ggagcgcatg ggctaatagg tagccaccta cctcgctgag caattagcga 480

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 540

481 aggtacgcgg cgtcattgtt aacgagttcg tctaaatagc ggtcgtcgag gcttatcgcg 540

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 600

541 ggaaggggaa cgggccgcaa ttactaaacg ggtttgtcca gcgactttac gccgaccacg 600

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 660

601 cgaagtaggc ccgctttctt ggggcataac cgtttataac tgccggtcaa ttcggtaagt 660

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... NruI(708) ..........

661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 720

661 acggtcatcc gcgcgcctgc tttcatttgg gtgaccacta tggtaagcgc tcggaggcct 720

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 780

721 actgctggca tcactactta gagaggaccg cccttgtcgt tttatagtgg gccagccgtt 780

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 840

781 tgtttaagag cagggactaa aaagtggtgg gggactggcg cttaccactc taactcttat 840

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 900

841 attggaaagt aagggtcgcc agccagctat ttttttagct ctattggcaa ccggagttag 900

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 960

901 ccgcaatttg ggcggtggtc tacccgtaat ttgctcatag ggccgtcgtc ccctagtaaa 960

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 1020

961 acgcgaagtc ggtatgaaaa gtatgagggc ggtaagtctc ttctttggtt aacaggtata 1020

AraC .......... ORF 3 .......... .......... araO2

.......... .......... .......... .......... .......... AgeI(1074)

1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 1080

1021 acgtagtctg taacggcagt gacgcagaaa atgaccgaga agagcgattg gtttggccat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 1140

1081 tggggcgaat aattttcgta agacattgtt tcgccctggt ttcggtactg tttttgcgca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 1200

1141 ttgttttcac agatattagt gccgtctttt caggtgtaac taataaacgt gccgcagtgt 1200

.......... .......... araO1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 1260

1201 gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc taggatggac tgcgaaaaat 1260

CAP_BS pBAD_fwd_pri ........ AraI1I2 .......... ARA_pro

.......... .......... .......... .......... .......... ..........

1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt 1320

1261 agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc gatcgcttaa gctcgagcca 1320

ARA_pro .......... .......... .......... .......... ..........

EcoRI SacI KpnI,SmaI XbaI SalI1362) HindIII(1380)

1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga 1380

1321 tgggccccta ggagatctca gctggacgtc cgtacgttcg aaccgacaaa accgcctact 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag 1440

1381 ctcttctaaa agtcggacta tgtctaattt agtcttgcgt cttcgccaga ctattttgtc 1440

.......... .......... .......... . pBAD_rev_pri pTrcHis_rev_pri .

.......... .......... .......... .......... .......... ..........

1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg 1500

1441 ttaaacggac cgccgtcatc gcgccaccag ggtggactgg ggtacggctt gagtcttcac 1500

.......... .......... .......... .......... rrnB_term ..........

.......... .......... .......... .......... .......... ..........

1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag 1560

1501 tttgcggcat cgcggctacc atcacacccc agaggggtac gctctcatcc cttgacggtc 1560

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1620

1561 cgtagtttat tttgctttcc gagtcagctt tctgacccgg aaagcaaaat agacaacaaa 1620

.......... .......... rrnB_term ......... rrnB_T1_term .........

.......... .......... .......... .......... .......... ..........

1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa 1680

1621 cagccacttg cgagaggact catcctgttt aggcggccct cgcctaaact tgcaacgctt 1680

rrnB_T1_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa 1740

1681 cgttgccggg cctcccaccg cccgtcctgc gggcggtatt tgacggtccg tagtttaatt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt 1800

1741 cgtcttccgg taggactgcc taccggaaaa acgcaaagat gtttgagaaa acaaataaaa 1800

.......... .......... ......... rrnB_T2_term ......... ..........

.......... .......... .......... .......... .......... ..........

1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1860

1801 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1860

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1920

1861 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1920

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1980

1921 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 2040

1981 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 2040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2100

2041 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 2160

2101 atacaccgcg ccataatagg gcacaactgc ggcccgttct cgttgagcca gcggcgtatg 2160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2220

2161 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2280

2221 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2340

2281 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2400

2341 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg 2460

2401 tgctcgcact gtggtgctac ggacgtcgtt accgttgttg caacgcgttt gataattgac 2460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

FspI(2464) .......... .......... .......... .......... AseI(2512)

2461 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2520

2461 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 2520

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2580

2521 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2580

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2640

2581 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2640

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 2700

2641 gggcatagca tcaatagatg tgctgcccct cagtccgttg atacctactt gctttatctg 2700

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2760

2701 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2760

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 2820

2761 gtatatatga aatctaacta aatgcgcggg acatcgccgc gtaattcgcg ccgcccacac 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 2880

2821 caccaatgcg cgtcgcactg gcgatgtgaa cggtcgcggg atcgcgggcg aggaaagcga 2880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 2940

2881 aagaagggaa ggaaagagcg gtgcaagcgg ccgaaagggg cagttcgaga tttagccccc 2940

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg 3000

2941 gagggaaatc ccaaggctaa atcacgaaat gccgtggagc tggggttttt tgaactaaac 3000

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 3060

3001 ccactaccaa gtgcatcacc cggtagcggg actatctgcc aaaaagcggg aaactgcaac 3060

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc 3120

3061 ctcaggtgca agaaattatc acctgagaac aaggtttgaa cttgttgtga gttgggatag 3120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 3180

3121 agcccgataa gaaaactaaa tattccctaa aacggctaaa gccggataac caatttttta 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa 3240

3181 ctcgactaaa ttgtttttaa attgcgctta aaattgtttt ataattgcaa atgttaaatt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 3300

3241 ttcctagatc cacttctagg aaaaactatt agagtactgg ttttagggaa ttgcactcaa 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 3360

3301 aagcaaggtg actcgcagtc tggggcatct tttctagttt cctagaagaa ctctaggaaa 3360

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 3420

3361 aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc gccaccaaac 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 3480

3421 aaacggccta gttctcgatg gttgagaaaa aggcttccat tgaccgaagt cgtctcgcgt 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 3540

3481 ctatggttta tgacaggaag atcacatcgg catcaatccg gtggtgaagt tcttgagaca 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 3600

3541 tcgtggcgga tgtatggagc gagacgatta ggacaatggt caccgacgac ggtcaccgct 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 3660

3601 attcagcaca gaatggccca acctgagttc tgctatcaat ggcctattcc gcgtcgccag 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 3720

3661 cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga tgtggcttga 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 3780

3721 ctctatggat gtcgcactcg atactctttc gcggtgcgaa gggcttccct ctttccgcct 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 3840

3781 gtccataggc cattcgccgt cccagccttg tcctctcgcg tgctccctcg aaggtccccc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 3900

3841 tttgcggacc atagaaatat caggacagcc caaagcggtg gagactgaac tcgcagctaa 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 3960

3901 aaacactacg agcagtcccc ccgcctcgga tacctttttg cggtcgttgc gccggaaaaa 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 4020

3961 tgccaaggac cggaaaacga ccggaaaacg agtgtacaag aaaggacgca ataggggact 4020

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 4080

4021 aagacaccta ttggcataat ggcggaaact cactcgacta tggcgagcgg cgtcggcttg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct 4140

4081 ctggctcgcg tcgctcagtc actcgctcct tcgccttctc gcggactacg ccataaaaga 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NdeI(4191)

4141 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc 4200

4141 ggaatgcgta gacacgccat aaagtgtggc gtataccacg tgagagtcat gttagacgag 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct 4260

4201 actacggcgt atcaattcgg tcatatgtga ggcgatagcg atgcactgac ccagtaccga 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca 4320

4261 cgcggggctg tgggcggttg tgggcgactg cgcgggactg cccgaacaga cgagggccgt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg 4380

4321 aggcgaatgt ctgttcgaca ctggcagagg ccctcgacgt acacagtctc caaaagtggc 4380

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

4381 tcatcaccga aacgcgcgag gcagcaagga gatggcgccc aacagtcccc cggccacggg 4440

4381 agtagtggct ttgcgcgctc cgtcgttcct ctaccgcggg ttgtcagggg gccggtgccc 4440

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc 4500

4441 cggacggtgg tatgggtgcg gctttgttcg cgagtactcg ggcttcaccg ctcgggctag 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat 4560

4501 aaggggtagc cactacagcc gctatatccg cggtcgttgg cgtggacacc gcggccacta 4560

.......... .......... ........ pBRrevBam_pri ........ ..........

.......... .......... .......... .......... .......... ..........

4561 gccggccacg atgcgtccgg cgtagaggat ctgctcatgt ttgacagctt atc....... 4613

4561 cggccggtgc tacgcaggcc gcatctccta gacgagtaca aactgtcgaa tag....... 4613

.......... .......... .......... .......... .......... ..........