Sequence of pBAD202_D

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Sequence of pBAD202_D_LacZ

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Sequence of pBAD202/D/LacZ : 7520 BP; 1762 A; 2047 C; 2009 G; 1702 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... .......... .......... .......... NcoI(344) ..........

301 ctagaaataa ttttgtttaa ctttaagaag gagatataca tacccatggg atctgataaa 360

301 gatctttatt aaaacaaatt gaaattcttc ctctatatgt atgggtaccc tagactattt 360

.......... ....... T7_transl_en_RBS ....... ..... His_patch_thio ORF 1

.......... .......... .......... .......... .......... ..........

361 attattcatc tgactgatga ttcttttgat actgatgtac ttaaggcaga tggtgcaatc 420

361 taataagtag actgactact aagaaaacta tgactacatg aattccgtct accacgttag 420

.......... ..... His_patch_thio ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctggttgatt tctgggcaca ctggtgcggt ccgtgcaaaa tgatcgctcc gattctggat 480

421 gaccaactaa agacccgtgt gaccacgcca ggcacgtttt actagcgagg ctaagaccta 480

.......... ..... His_patch_thio ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcacaac 540

481 ctttagcgac tgcttatagt cccgtttgac tggcaacgtt ttgacttgta gctagtgttg 540

.......... ..... His_patch_thio ORF 1 ..... .......... ..........

SmaI(542) .......... .......... .......... .......... ..........

541 ccgggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac 600

541 ggcccgtgac gcggctttat accgtaggca ccatagggct gagacgacga caagtttttg 600

.......... ..... His_patch_thio ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc 660

601 ccacttcacc gccgttggtt tcacccacgt gacagatttc cagtcaactt tctcaaggag 660

.......... ..... His_patch_thio ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gacgctaacc tggccggctc tggatccggt gatgacgatg acaagctggg aattgatccc 720

661 ctgcgattgg accggccgag acctaggcca ctactgctac tgttcgaccc ttaactaggg 720

His_patch_thio ...... ORF 1 EK .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttcaccaaga tgatagatcc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 780

721 aagtggttct actatctagg gcagcaaaat gttgcagcac tgaccctttt gggaccgcaa 780

.......... ....... ORF 1 M13_pUC_fwd_pri lacZ .......

.......... .......... .......... .......... .......... ..........

781 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 840

781 tgggttgaat tagcggaacg tcgtgtaggg ggaaagcggt cgaccgcatt atcgcttctc 840

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctttgcc 900

841 cgggcgtggc tagcgggaag ggttgtcaac gcgtcggact taccgcttac cgcgaaacgg 900

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... KpnI(913) .......... .......... .......... ..........

901 tggtttccgg taccagaagc ggtgccggaa agctggctgg agtgcgatct tcctgaggcc 960

901 accaaaggcc atggtcttcg ccacggcctt tcgaccgacc tcacgctaga aggactccgg 960

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gatactgtcg tcgtcccctc aaactggcag atgcacggtt acgatgcgcc catctacacc 1020

961 ctatgacagc agcaggggag tttgaccgtc tacgtgccaa tgctacgcgg gtagatgtgg 1020

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aacgtaacct atcccattac ggtcaatccg ccgtttgttc ccacggagaa tccgacgggt 1080

1021 ttgcattgga tagggtaatg ccagttaggc ggcaaacaag ggtgcctctt aggctgccca 1080

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgttactcgc tcacatttaa tgttgatgaa agctggctac aggaaggcca gacgcgaatt 1140

1081 acaatgagcg agtgtaaatt acaactactt tcgaccgatg tccttccggt ctgcgcttaa 1140

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 atttttgatg gcgttaactc ggcgtttcat ctgtggtgca acgggcgctg ggtcggttac 1200

1141 taaaaactac cgcaattgag ccgcaaagta gacaccacgt tgcccgcgac ccagccaatg 1200

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggccaggaca gtcgtttgcc gtctgaattt gacctgagcg catttttacg cgccggagaa 1260

1201 ccggtcctgt cagcaaacgg cagacttaaa ctggactcgc gtaaaaatgc gcggcctctt 1260

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aaccgcctcg cggtgatggt gctgcgttgg agtgacggca gttatctgga agatcaggat 1320

1261 ttggcggagc gccactacca cgacgcaacc tcactgccgt caatagacct tctagtccta 1320

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... ......... AatII(1352) ......... ..........

1321 atgtggcgga tgagcggcat tttccgtgac gtctcgttgc tgcataaacc gactacacaa 1380

1321 tacaccgcct actcgccgta aaaggcactg cagagcaacg acgtatttgg ctgatgtgtt 1380

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atcagcgatt tccatgttgc cactcgcttt aatgatgatt tcagccgcgc tgtactggag 1440

1381 tagtcgctaa aggtacaacg gtgagcgaaa ttactactaa agtcggcgcg acatgacctc 1440

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gctgaagttc agatgtgcgg cgagttgcgt gactacctac gggtaacagt ttctttatgg 1500

1441 cgacttcaag tctacacgcc gctcaacgca ctgatggatg cccattgtca aagaaatacc 1500

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cagggtgaaa cgcaggtcgc cagcggcacc gcgcctttcg gcggtgaaat tatcgatgag 1560

1501 gtcccacttt gcgtccagcg gtcgccgtgg cgcggaaagc cgccacttta atagctactc 1560

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cgtggtggtt atgccgatcg cgtcacacta cgtctgaacg tcgaaaaccc gaaactgtgg 1620

1561 gcaccaccaa tacggctagc gcagtgtgat gcagacttgc agcttttggg ctttgacacc 1620

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agcgccgaaa tcccgaatct ctatcgtgcg gtggttgaac tgcacaccgc cgacggcacg 1680

1621 tcgcggcttt agggcttaga gatagcacgc caccaacttg acgtgtggcg gctgccgtgc 1680

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ctgattgaag cagaagcctg cgatgtcggt ttccgcgagg tgcggattga aaatggtctg 1740

1681 gactaacttc gtcttcggac gctacagcca aaggcgctcc acgcctaact tttaccagac 1740

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctgctgctga acggcaagcc gttgctgatt cgaggcgtta accgtcacga gcatcatcct 1800

1741 gacgacgact tgccgttcgg caacgactaa gctccgcaat tggcagtgct cgtagtagga 1800

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctgcatggtc aggtcatgga tgagcagacg atggtgcagg atatcctgct gatgaagcag 1860

1801 gacgtaccag tccagtacct actcgtctgc taccacgtcc tataggacga ctacttcgtc 1860

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aacaacttta acgccgtgcg ctgttcgcat tatccgaacc atccgctgtg gtacacgctg 1920

1861 ttgttgaaat tgcggcacgc gacaagcgta ataggcttgg taggcgacac catgtgcgac 1920

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgcgaccgct acggcctgta tgtggtggat gaagccaata ttgaaaccca cggcatggtg 1980

1921 acgctggcga tgccggacat acaccaccta cttcggttat aactttgggt gccgtaccac 1980

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ccaatgaatc gtctgaccga tgatccgcgc tggctaccgg cgatgagcga acgcgtaacg 2040

1981 ggttacttag cagactggct actaggcgcg accgatggcc gctactcgct tgcgcattgc 2040

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... BclI(2075) .......... ..........

2041 cgaatggtgc agcgcgatcg taatcacccg agtgtgatca tctggtcgct ggggaatgaa 2100

2041 gcttaccacg tcgcgctagc attagtgggc tcacactagt agaccagcga ccccttactt 2100

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tcaggccacg gcgctaatca cgacgcgctg tatcgctgga tcaaatctgt cgatccttcc 2160

2101 agtccggtgc cgcgattagt gctgcgcgac atagcgacct agtttagaca gctaggaagg 2160

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgcccggtgc agtatgaagg cggcggagcc gacaccacgg ccaccgatat tatttgcccg 2220

2161 gcgggccacg tcatacttcc gccgcctcgg ctgtggtgcc ggtggctata ataaacgggc 2220

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atgtacgcgc gcgtggatga agaccagccc ttcccggctg tgccgaaatg gtccatcaaa 2280

2221 tacatgcgcg cgcacctact tctggtcggg aagggccgac acggctttac caggtagttt 2280

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaatggcttt cgctacctgg agagacgcgc ccgctgatcc tttgcgaata cgcccacgcg 2340

2281 tttaccgaaa gcgatggacc tctctgcgcg ggcgactagg aaacgcttat gcgggtgcgc 2340

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 atgggtaaca gtcttggcgg tttcgctaaa tactggcagg cgtttcgtca gtatccccgt 2400

2341 tacccattgt cagaaccgcc aaagcgattt atgaccgtcc gcaaagcagt cataggggca 2400

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttacagggcg gcttcgtctg ggactgggtg gatcagtcgc tgattaaata tgatgaaaac 2460

2401 aatgtcccgc cgaagcagac cctgacccac ctagtcagcg actaatttat actacttttg 2460

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggcaacccgt ggtcggctta cggcggtgat tttggcgata cgccgaacga tcgccagttc 2520

2461 ccgttgggca ccagccgaat gccgccacta aaaccgctat gcggcttgct agcggtcaag 2520

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgtatgaacg gtctggtctt tgccgaccgc acgccgcatc cagcgctgac ggaagcaaaa 2580

2521 acatacttgc cagaccagaa acggctggcg tgcggcgtag gtcgcgactg ccttcgtttt 2580

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 caccagcagc agtttttcca gttccgttta tccgggcaaa ccatcgaagt gaccagcgaa 2640

2581 gtggtcgtcg tcaaaaaggt caaggcaaat aggcccgttt ggtagcttca ctggtcgctt 2640

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tacctgttcc gtcatagcga taacgagctc ctgcactgga tggtggcgct ggatggtaag 2700

2641 atggacaagg cagtatcgct attgctcgag gacgtgacct accaccgcga cctaccattc 2700

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgattgaa 2760

2701 ggcgaccgtt cgccacttca cggagaccta cagcgaggtg ttccatttgt caactaactt 2760

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctgcctgaac taccgcagcc ggagagcgcc gggcaactct ggctcacagt acgcgtagtg 2820

2761 gacggacttg atggcgtcgg cctctcgcgg cccgttgaga ccgagtgtca tgcgcatcac 2820

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 caaccgaacg cgaccgcatg gtcagaagcc gggcacatca gcgcctggca gcagtggcgt 2880

2821 gttggcttgc gctggcgtac cagtcttcgg cccgtgtagt cgcggaccgt cgtcaccgca 2880

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctggcggaaa acctcagtgt gacgctcccc gccgcgtccc acgccatccc gcatctgacc 2940

2881 gaccgccttt tggagtcaca ctgcgagggg cggcgcaggg tgcggtaggg cgtagactgg 2940

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 accagcgaaa tggatttttg catcgagctg ggtaataagc gttggcaatt taaccgccag 3000

2941 tggtcgcttt acctaaaaac gtagctcgac ccattattcg caaccgttaa attggcggtc 3000

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcaggctttc tttcacagat gtggattggc gataaaaaac aactgctgac gccgctgcgc 3060

3001 agtccgaaag aaagtgtcta cacctaaccg ctattttttg ttgacgactg cggcgacgcg 3060

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gatcagttca cccgtgcacc gctggataac gacattggcg taagtgaagc gacccgcatt 3120

3061 ctagtcaagt gggcacgtgg cgacctattg ctgtaaccgc attcacttcg ctgggcgtaa 3120

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gaccctaacg cctgggtcga acgctggaag gcggcgggcc attaccaggc cgaagcagcg 3180

3121 ctgggattgc ggacccagct tgcgaccttc cgccgcccgg taatggtccg gcttcgtcgc 3180

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttgttgcagt gcacggcaga tacacttgct gatgcggtgc tgattacgac cgctcacgcg 3240

3181 aacaacgtca cgtgccgtct atgtgaacga ctacgccacg actaatgctg gcgagtgcgc 3240

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tggcagcatc aggggaaaac cttatttatc agccggaaaa cctaccggat tgatggtagt 3300

3241 accgtcgtag tccccttttg gaataaatag tcggcctttt ggatggccta actaccatca 3300

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggtcaaatgg cgattaccgt tgatgttgaa gtggcgagcg atacaccgca tccggcgcgg 3360

3301 ccagtttacc gctaatggca actacaactt caccgctcgc tatgtggcgt aggccgcgcc 3360

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 attggcctga actgccagct ggcgcaggta gcagagcggg taaactggct cggattaggg 3420

3361 taaccggact tgacggtcga ccgcgtccat cgtctcgccc atttgaccga gcctaatccc 3420

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ccgcaagaaa actatcccga ccgccttact gccgcctgtt ttgaccgctg ggatctgcca 3480

3421 ggcgttcttt tgatagggct ggcggaatga cggcggacaa aactggcgac cctagacggt 3480

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttgtcagaca tgtatacccc gtacgtcttc ccgagcgaaa acggtctgcg ctgcgggacg 3540

3481 aacagtctgt acatatgggg catgcagaag ggctcgcttt tgccagacgc gacgccctgc 3540

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgcgaattga attatggccc acaccagtgg cgcggcgact tccagttcaa catcagccgc 3600

3541 gcgcttaact taataccggg tgtggtcacc gcgccgctga aggtcaagtt gtagtcggcg 3600

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tacagtcaac agcaactgat ggaaaccagc catcgccatc tgctgcacgc ggaagaaggc 3660

3601 atgtcagttg tcgttgacta cctttggtcg gtagcggtag acgacgtgcg ccttcttccg 3660

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 acatggctga atatcgacgg tttccatatg gggattggtg gcgacgactc ctggagcccg 3720

3661 tgtaccgact tatagctgcc aaaggtatac ccctaaccac cgctgctgag gacctcgggc 3720

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcagtatcgg cggaattcca gctgagcgcc ggtcgctacc attaccagtt ggtctggtgt 3780

3721 agtcatagcc gccttaaggt cgactcgcgg ccagcgatgg taatggtcaa ccagaccaca 3780

.......... .......... ORF 1 lacZ .......... .......... ..........

NotI(3788) XhoI(3794) ........ HindIII(3811) ........ ..........

3781 caaaaagcgg ccgctcgagt cggcgagctc aagcttgaag gtaagcctat ccctaaccct 3840

3781 gtttttcgcc ggcgagctca gccgctcgag ttcgaacttc cattcggata gggattggga 3840

.......... .......... ORF 1 lacZ .......... V5 ..........

.......... .......... .......... .......... .......... ..........

3841 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacg 3900

3841 gaggagccag agctaagatg cgcatggcca gtagtagtgg tagtggtaac tcaaatttgc 3900

lacZ V5 ORF 1 6xHis .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtctccagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat 3960

3901 cagaggtcga accgacaaaa ccgcctactc tcttctaaaa gtcggactat gtctaattta 3960

.......... .......... .......... .......... . pBAD_rev_pri pTrcHis_rev_pri

.......... .......... .......... .......... .......... ..........

3961 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc 4020

3961 gtcttgcgtc ttcgccagac tattttgtct taaacggacc gccgtcatcg cgccaccagg 4020

.......... .......... .......... .......... rrnB_term ..........

.......... .......... .......... .......... .......... ..........

4021 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt 4080

4021 gtggactggg gtacggcttg agtcttcact ttgcggcatc gcggctacca tcacacccca 4080

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc t cagtcgaaa 4140

4081 gaggggtacg ctctcatccc ttgacggtcc gtagtttatt ttgctttccg agtcagcttt 4140

.......... .......... rrnB_term ......... rrnB_T1_term .........

.......... .......... .......... .......... .......... ..........

4141 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat 4200

4141 ctgacccgga aagcaaaata gacaacaaac agccacttgc gagaggactc atcctgttta 4200

rrnB_term rrnB_T1_term ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc 4260

4201 ggcggccctc gcctaaactt gcaacgcttc gttgccgggc ctcccaccgc ccgtcctgcg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt 4320

4261 ggcggtattt gacggtccgt agtttaattc gtcttccggt aggactgcct accggaaaaa 4320

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

4321 gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat 4380

4321 cgcaaagatg tttgagaaaa caaataaaaa gatttatgta agtttataca taggcgagta 4380

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4381 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 4440

4381 ctctaatagt ttttcctaga agtggatcta ggaaaattta atttttactt caaaatttag 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aatctaaagt atatatgagt aaacttggtc tgacagttag gcgtcgcttg gtcggtcatt 4500

4441 ttagatttca tatatactca tttgaaccag actgtcaatc cgcagcgaac cagccagtaa 4500

.......... .......... .......... .......... .......... ..........

BstBI(4501) ........ .......... .......... .......... ..........

4501 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct 4560

4501 agcttggggt ctcagggcga gtcttcttga gcagttcttc cgctatcttc c gctacgcga 4560

.......... .......... ...... ORF 2 Neomycin(variant) ......

.......... .......... .......... .......... .......... ..........

4561 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa 4620

4561 cgcttagccc tcgccgctat ggcatttcgt gctccttcgc cagtcgggta agcggcggtt 4620

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca 4680

4621 cgagaagtcg ttatagtgcc catcggttgc gatacaggac tatcgccagg cggtgtgggt 4680

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc 4740

4681 cggccggtgt cagctactta ggtcttttcg ccggtaaaag gtggtactat aagccgttcg 4740

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aggcatcgcc atgtgtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg 4800

4741 tccgtagcgg tacacagtgc tgctctagga gcggcagccc gtacgcgcgg aactcggacc 4800

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa 4860

4801 gcttgtcaag ccgaccgcgc tcggggacta cgagaagcag gtctagtagg actagctgtt 4860

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gaccggcttc catccgagta cgtgcccgct cgatgcgatg tttcgcttgg tggtcgaatg 4920

4861 ctggccgaag gtaggctcat gcacgggcga gctacgctac aaagcgaacc accagcttac 4920

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt 4980

4921 ccgtccatcg gcctagttcg catacgtcgg cggcgtaacg tagtcggtac tacctatgaa 4980

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca 5040

4981 agagccgtcc tcgttccact ctactgtcct ctaggacggg gccgtgaagc gggttatcgt 5040

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg 5100

5041 cggtcaggga agggcgaagt cactgttgca gctcgtgtcg acgcgttcct tgcgggcagc 5100

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

MscI(5103) .......... .......... .......... .......... ..........

5101 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt 5160

5101 accggtcggt gctatcggcg cgacggagca ggacgtcaag taagtcccgt ggcctgtcca 5160

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... NarI(5183) .......... .......... ..........

5161 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag 5220

5161 gccagaactg tttttcttgg cccgcgggga cgcgactgtc ggccttgtgc cgccgtagtc 5220

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 agcagccgat tgtcagttgt gcccagtcat agccgaatag cctctccacc caagcggccg 5280

5221 tcgtcggcta acagtcaaca cgggtcagta tcggcttatc ggagaggtgg gttcgccggc 5280

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gagaacctgc gtgcaatcca tcttgttcaa tcatactctt cctttttcaa tattattgaa 5340

5281 ctcttggacg cacgttaggt agaacaagtt agtatgagaa ggaaaaagtt ataataactt 5340

... ORF 2 Neomycin(variant) ... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcatttatca gggttattgt ctcatgacca aaatccctta acgtgagttt tcgttccact 5400

5341 cgtaaatagt cccaataaca gagtactggt tttagggaat tgcactcaaa agcaaggtga 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 5460

5401 ctcgcagtct ggggcatctt ttctagtttc ctagaagaac tctaggaaaa aaagacgcgc 5460

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5461 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 5520

5461 attagacgac gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca aacggcctag 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 5580

5521 ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc tatggtttat 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 5640

5581 gacaggaaga tcacatcggc atcaatccgg tggtgaagtt cttgagacat cgtggcggat 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 5700

5641 gtatggagcg agacgattag gacaatggtc accgacgacg gtcaccgcta ttcagcacag 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 5760

5701 aatggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc ccgacttgcc 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 5820

5761 ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac tctatggatg 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 5880

5821 tcgcactcga tactctttcg cggtgcgaag ggcttccctc tttccgcctg tccataggcc 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 5940

5881 attcgccgtc ccagccttgt cctctcgcgt gctccctcga aggtccccct ttgcggacca 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 6000

5941 tagaaatatc aggacagccc aaagcggtgg agactgaact cgcagctaaa aacactacga 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 6060

6001 gcagtccccc cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat gccaaggacc 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 6120

6061 ggaaaacgac cggaaaacga gtgtacaaga aaggacgcaa taggggacta agacacctat 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 6180

6121 tggcataatg gcggaaactc actcgactat ggcgagcggc gtcggcttgc tggctcgcgt 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 6240

6181 cgctcagtca ctcgctcctt cgccttctcg cggactacgc cataaaagag gaatgcgtag 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat 6300

6241 acacgccata aagtgtggcg tataccacgt gagagtcatg ttagacgaga ctacggcgta 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca 6360

6301 tcaattcggt catatgtgag gcgatagcga tgcactgacc cagtaccgac gcggggctgt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 6420

6361 gggcggttgt gggcgactgc gcgggactgc ccgaacagac gagggccgta ggcgaatgtc 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 6480

6421 tgttcgacac tggcagaggc cctcgacgta cacagtctcc aaaagtggca gtagtggctt 6480

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg 6540

6481 tgcgcgctcc gtcgtctagt taagcgcgcg cttccgcttc gccgtacgta ttacacggac 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 tcaaatggac gaagcaggga ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt 6600

6541 agtttacctg cttcgtccct aagacgtttg ggatacgatg aggcagttcg gcagttaaca 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ctgattcgtt accaattatg acaacttgac ggctacatca ttcacttttt cttcacaacc 6660

6601 gactaagcaa tggttaatac tgttgaactg ccgatgtagt aagtgaaaaa gaagtgttgg 6660

.......... .......... .......... AraC .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg agaaatagag 6720

6661 ccgtgccttg agcgagcccg accggggcca cgtaaaaaat ttatgggcgc tctttatctc 6720

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa 6780

6721 aactagcagt tttggttgta acgctggctg ccaccgctat ccgtaggccc accacgagtt 6780

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc taatccctaa 6840

6781 ttcgtcgaag cggaccgact atgcaaccag gagcgcggtc gaattctgcg attagggatt 6840

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct gtgcgacgct 6900

6841 gacgaccgcc ttttctacac tgtctgcgct gccgctgttc gtttgtacga cacgctgcga 6900

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag cctcgcgtac 6960

6901 ccgctatagt tttaacgaca gacggtccac tagcgactac atgactgttc ggagcgcatg 6960

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa 7020

6961 ggctaatagg tagccaccta cctcgctgag caattagcga aggtacgcgg cgtcattgtt 7020

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt 7080

7021 aacgagttcg tctaaatagc ggtcgtcgag gcttatcgcg ggaaggggaa cgggccgcaa 7080

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa 7140

7081 ttactaaacg ggtttgtcca gcgactttac gccgaccacg cgaagtaggc ccgctttctt 7140

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg cgcgcggacg 7200

7141 ggggcataac cgtttataac tgccggtcaa ttcggtaagt acggtcatcc gcgcgcctgc 7200

.......... .......... AraC .......... .......... ..........

.......... .......... NruI(7228) .......... .......... ..........

7201 aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt agtgatgaat 7260

7201 tttcatttgg gtgaccacta tggtaagcgc tcggaggcct actgctggca tcactactta 7260

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc gtccctgatt 7320

7261 gagaggaccg cccttgtcgt tttatagtgg gccagccgtt tgtttaagag cagggactaa 7320

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca ttcccagcgg 7380

7321 aaagtggtgg gggactggcg cttaccactc taactcttat attggaaagt aagggtcgcc 7380

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag 7440

7381 agccagctat ttttttagct ctattggcaa ccggagttag ccgcaatttg ggcggtggtc 7440

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag ccatactttt 7500

7441 tacccgtaat ttgctcatag ggccgtcgtc ccctagtaaa acgcgaagtc ggtatgaaaa 7500

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 catactcccg ccattcagag .......... .......... .......... .......... 7520

7501 gtatgagggc ggtaagtctc .......... .......... .......... .......... 7520

.......... .......... .......... .......... .......... ..........