wJwJ!LOCUS pBAD202 Directional TOPO 4448 bp DNA SYN 07-Sep-2007 DEFINITION pBAD202 Directional TOPO ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4448 /organism="pBAD202 Directional TOPO" /mol_type="other DNA" CDS complement(3544..25) /label="ORF frame 1" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 4..19 /label="araO2" misc_feature 161..172 /label="araO1" misc_feature 203..216 /label="CAP_BS" misc_feature 208..227 /label="pBAD_fwd_primer" misc_feature 213..251 /label="AraI1I2" promoter 248..276 /label="ARA_promoter" misc_feature 317..333 /label="T7_transl_en_RBS" misc_feature 346..674 /label="His_patch_thio" misc_feature 691..705 /label="EK" misc_feature 748..789 /label="V5" misc_feature 799..816 /label="6xHis" misc_feature complement(872..889) /label="pBAD_rev_primer" misc_feature complement(872..889) /label="pTrcHis_rev_primer" terminator 922..1079 /label="rrnB_terminator" terminator 1045..1088 /label="rrnB_T1_terminator" terminator 1220..1247 /label="rrnB_T2_terminator" promoter 1288..1311 /label="AmpR_promoter" gene complement(1451..2230) /label="Neomycin(variant)" /gene="Neomycin(variant)" CDS complement(1448..2242) /label="ORF frame 2" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQLTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" rep_origin 2350..2969 /label="pBR322_origin" misc_feature 3366..3388 /label="pGEX_3_primer" misc_feature complement(3544..4422) /label="AraC" ORIGIN 1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT 61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA 121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG 181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG 241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG 301 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TACCCATGGG ATCTGATAAA 361 ATTATTCATC TGACTGATGA TTCTTTTGAT ACTGATGTAC TTAAGGCAGA TGGTGCAATC 421 CTGGTTGATT TCTGGGCACA CTGGTGCGGT CCGTGCAAAA TGATCGCTCC GATTCTGGAT 481 GAAATCGCTG ACGAATATCA GGGCAAACTG ACCGTTGCAA AACTGAACAT CGATCACAAC 541 CCGGGCACTG CGCCGAAATA TGGCATCCGT GGTATCCCGA CTCTGCTGCT GTTCAAAAAC 601 GGTGAAGTGG CGGCAACCAA AGTGGGTGCA CTGTCTAAAG GTCAGTTGAA AGAGTTCCTC 661 GACGCTAACC TGGCCGGCTC TGGATCCGGT GATGACGATG ACAAGCTGGG AATTGATCCC 721 TTCACCAAGG GCGAGCTCAA GCTTGAAGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 781 GATTCTACGC GTACCGGTCA TCATCACCAT CACCATTGAG TTTAAACGGT CTCCAGCTTG 841 GCTGTTTTGG CGGATGAGAG AAGATTTTCA GCCTGATACA GATTAAATCA GAACGCAGAA 901 GCGGTCTGAT AAAACAGAAT TTGCCTGGCG GCAGTAGCGC GGTGGTCCCA CCTGACCCCA 961 TGCCGAACTC AGAAGTGAAA CGCCGTAGCG CCGATGGTAG TGTGGGGTCT CCCCATGCGA 1021 GAGTAGGGAA CTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT 1081 CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG 1141 GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC GCCATAAACT 1201 GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG GCCTTTTTGC GTTTCTACAA 1261 ACTCTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GATTATCAAA 1321 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 1381 ATATGAGTAA ACTTGGTCTG ACAGTTAGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA 1441 GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG 1501 CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA 1561 TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGGTCCGC CACACCCAGC CGGCCACAGT 1621 CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT 1681 GTGTCACGAC GAGATCCTCG CCGTCGGGCA TGCGCGCCTT GAGCCTGGCG AACAGTTCGG 1741 CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA 1801 TCCGAGTACG TGCCCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG 1861 GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG 1921 CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC 1981 CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG 2041 ATAGCCGCGC TGCCTCGTCC TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA 2101 AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG 2161 TCAGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT 2221 GCAATCCATC TTGTTCAATC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 2281 GTTATTGTCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC 2341 CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT 2401 TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA 2461 CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG 2521 TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC 2581 TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG 2641 ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA 2701 CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT 2761 GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG 2821 TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC 2881 CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC 2941 GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC 3001 CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG 3061 CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA 3121 GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT 3181 CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT 3241 ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC 3301 CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC 3361 CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA 3421 GCAGATCAAT TCGCGCGCGA AGGCGAAGCG GCATGCATAA TGTGCCTGTC AAATGGACGA 3481 AGCAGGGATT CTGCAAACCC TATGCTACTC CGTCAAGCCG TCAATTGTCT GATTCGTTAC 3541 CAATTATGAC AACTTGACGG CTACATCATT CACTTTTTCT TCACAACCGG CACGGAACTC 3601 GCTCGGGCTG GCCCCGGTGC ATTTTTTAAA TACCCGCGAG AAATAGAGTT GATCGTCAAA 3661 ACCAACATTG CGACCGACGG TGGCGATAGG CATCCGGGTG GTGCTCAAAA GCAGCTTCGC 3721 CTGGCTGATA CGTTGGTCCT CGCGCCAGCT TAAGACGCTA ATCCCTAACT GCTGGCGGAA 3781 AAGATGTGAC AGACGCGACG GCGACAAGCA AACATGCTGT GCGACGCTGG CGATATCAAA 3841 ATTGCTGTCT GCCAGGTGAT CGCTGATGTA CTGACAAGCC TCGCGTACCC GATTATCCAT 3901 CGGTGGATGG AGCGACTCGT TAATCGCTTC CATGCGCCGC AGTAACAATT GCTCAAGCAG 3961 ATTTATCGCC AGCAGCTCCG AATAGCGCCC TTCCCCTTGC CCGGCGTTAA TGATTTGCCC 4021 AAACAGGTCG CTGAAATGCG GCTGGTGCGC TTCATCCGGG CGAAAGAACC CCGTATTGGC 4081 AAATATTGAC GGCCAGTTAA GCCATTCATG CCAGTAGGCG CGCGGACGAA AGTAAACCCA 4141 CTGGTGATAC CATTCGCGAG CCTCCGGATG ACGACCGTAG TGATGAATCT CTCCTGGCGG 4201 GAACAGCAAA ATATCACCCG GTCGGCAAAC AAATTCTCGT CCCTGATTTT TCACCACCCC 4261 CTGACCGCGA ATGGTGAGAT TGAGAATATA ACCTTTCATT CCCAGCGGTC GGTCGATAAA 4321 AAAATCGAGA TAACCGTTGG CCTCAATCGG CGTTAAACCC GCCACCAGAT GGGCATTAAA 4381 CGAGTATCCC GGCAGCAGGG GATCATTTTG CGCTTCAGCC ATACTTTTCA TACTCCCGCC 4441 ATTCAGAG // 0pBAD202 Directional TOPO "Times New Roman"wJC0 ORF 1"Times New RomanwJC0araO2"Times New RomanwJCɆ0 AraC"Times New RomanwJCɆ0araO1"Times New RomanwJ0CAP_BS"Times New RomanwJC0 pBAD_fwd_pri"Times New RomanwJC0AraI1I2"Times New Roman*wJCɆ0ARA_pro"Times New RomanwJ0=MT7_transl_en_RBS"Times New RomanwJC0ZHis_patch_thio"Times New RomanwJC0EK"Times New RomanwJCɆ0V5"Times New RomanwJ006xHis"Times New RomanwJC0hy pBAD_rev_pri"Times New RomanwJC0hypTrcHis_rev_pri"Times New RomanwJCɆ07 rrnB_term"Times New RomanwJ0@ rrnB_T1_term"Times New RomanwJC0 rrnB_T2_term"Times New RomanwJC0AmpR_pro"Times New RomanwJ0ORF 2"Times New Roman(wJCɆ0Neomycin(variant)"Times New RomanwJC0.  pBR322_origin"Times New RomanwJC0& <  pGEX_3_pri"Times New Roman wJNcoI"Times New RomanX0o` o wJClaI(530),SmaI(542)"Times New Roman0u\ wJSacI(737),HindIII(739)"Times New Roman0-<KK wJBstBI"Times New Roman04CSS wJ7PvuII(2007),FspI(2011),MscI(2031),PstI(2064),NarI(2111)"Times New Roman0 **u wJEagI"Times New Roman0||#|5]5D wJNdeI"Times New Romanu 0gjmOft wJEcoRV"Times New Roman0 wJNruI"Times New Roman<05.s(b SGb wJtest"Times New Roman0d wJtest"Times New Romanp0>Pj jA