Sequence of pBAD202_Directional

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Sequence of pBAD202_Directional_TOPO

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Sequence of pBAD202 Directional TOPO : 4448 BP; 1087 A; 1205 C; 1120 G; 1036 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... .......... .......... .......... NcoI(344) ..........

301 ctagaaataa ttttgtttaa ctttaagaag gagatataca tacccatggg atctgataaa 360

301 gatctttatt aaaacaaatt gaaattcttc ctctatatgt atgggtaccc tagactattt 360

.......... ....... T7_transl_en_RBS ....... ........ His_patch_thio

.......... .......... .......... .......... .......... ..........

361 attattcatc tgactgatga ttcttttgat actgatgtac ttaaggcaga tggtgcaatc 420

361 taataagtag actgactact aagaaaacta tgactacatg aattccgtct accacgttag 420

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctggttgatt tctgggcaca ctggtgcggt ccgtgcaaaa tgatcgctcc gattctggat 480

421 gaccaactaa agacccgtgt gaccacgcca ggcacgtttt actagcgagg ctaagaccta 480

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcacaac 540

481 ctttagcgac tgcttatagt cccgtttgac tggcaacgtt ttgacttgta gctagtgttg 540

.......... ........ His_patch_thio ........ .......... ..........

SmaI(542) .......... .......... .......... .......... ..........

541 ccgggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac 600

541 ggcccgtgac gcggctttat accgtaggca ccatagggct gagacgacga caagtttttg 600

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc 660

601 ccacttcacc gccgttggtt tcacccacgt gacagatttc cagtcaactt tctcaaggag 660

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 gacgctaacc tggccggctc tggatccggt gatgacgatg acaagctggg aattgatccc 720

661 ctgcgattgg accggccgag acctaggcca ctactgctac tgttcgaccc ttaactaggg 720

His_patch_thio ...... .......... EK .......... ..........

.... SacI(737),HindIII(739) .... .......... .......... ..........

721 ttcaccaagg gcgagctcaa gcttgaaggt aagcctatcc ctaaccctct cctcggtctc 780

721 aagtggttcc cgctcgagtt cgaacttcca ttcggatagg gattgggaga ggagccagag 780

.......... .......... .......... .......... V5 ..........

.......... .......... .......... .......... .......... ..........

781 gattctacgc gtaccggtca tcatcaccat caccattgag tttaaacggt ctccagcttg 840

781 ctaagatgcg catggccagt agtagtggta gtggtaactc aaatttgcca gaggtcgaac 840

V5 .......... 6xHis .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa 900

841 cgacaaaacc gcctactctc ttctaaaagt cggactatgt ctaatttagt cttgcgtctt 900

.......... .......... . pBAD_rev_pri pTrcHis_rev_pri . ..........

.......... .......... .......... .......... .......... ..........

901 gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca 960

901 cgccagacta ttttgtctta aacggaccgc cgtcatcgcg ccaccagggt ggactggggt 960

.......... .......... .......... .......... rrnB_term ..........

.......... .......... .......... .......... .......... ..........

961 tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga 1020

961 acggcttgag tcttcacttt gcggcatcgc ggctaccatc acaccccaga ggggtacgct 1020

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1080

1021 ctcatccctt gacggtccgt agtttatttt gctttccgag tcagctttct gacccggaaa 1080

.......... .......... rrnB_term ......... rrnB_T1_term .........

.......... .......... .......... .......... .......... ..........

1081 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg 1140

1081 gcaaaataga caacaaacag ccacttgcga gaggactcat cctgtttagg cggccctcgc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact 1200

1141 ctaaacttgc aacgcttcgt tgccgggcct cccaccgccc gtcctgcggg cggtatttga 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa 1260

1201 cggtccgtag tttaattcgt cttccggtag gactgcctac cggaaaaacg caaagatgtt 1260

.......... .......... ......... rrnB_T2_term ......... ..........

.......... .......... .......... .......... .......... ..........

1261 actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gattatcaaa 1320

1261 tgagaaaaca aataaaaaga tttatgtaag tttatacata ggcgagtact ctaatagttt 1320

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 1380

1321 ttcctagaag tggatctagg aaaatttaat ttttacttca aaatttagtt agatttcata 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... BstBI(1429) .........

1381 atatgagtaa acttggtctg acagttaggc gtcgcttggt cggtcatttc gaaccccaga 1440

1381 tatactcatt tgaaccagac tgtcaatccg cagcgaacca gccagtaaag cttggggtct 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag 1500

1441 cagggcgagt cttcttgagc agttcttccg ctatcttccg ctacgcgacg cttagccctc 1500

.......... .......... ... ORF 2 Neomycin(variant) ... ..........

.......... .......... .......... .......... .......... ..........

1501 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa 1560

1501 gccgctatgg catttcgtgc tccttcgcca gtcgggtaag cggcggttcg agaagtcgtt 1560

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt 1620

1561 atagtgccca tcggttgcga tacaggacta tcgccaggcg gtgtgggtcg gccggtgtca 1620

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat 1680

1621 gctacttagg tcttttcgcc ggtaaaaggt ggtactataa gccgttcgtc cgtagcggta 1680

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gtgtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg 1740

1681 cacagtgctg ctctaggagc ggcagcccgt acgcgcggaa ctcggaccgc ttgtcaagcc 1740

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca 1800

1741 gaccgcgctc ggggactacg agaagcaggt ctagtaggac tagctgttct ggccgaaggt 1800

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tccgagtacg tgcccgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg 1860

1801 aggctcatgc acgggcgagc tacgctacaa agcgaaccac cagcttaccc gtccatcggc 1860

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag 1920

1861 ctagttcgca tacgtcggcg gcgtaacgta gtcggtacta cctatgaaag agccgtcctc 1920

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc 1980

1921 gttccactct actgtcctct aggacggggc cgtgaagcgg gttatcgtcg gtcagggaag 1980

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .... PvuII(2007),FspI(2011) .... ..........

1981 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg 2040

1981 ggcgaagtca ctgttgcagc tcgtgtcgac gcgttccttg cgggcagcac cggtcggtgc 2040

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa 2100

2041 tatcggcgcg acggagcagg acgtcaagta agtcccgtgg cctgtccagc cagaactgtt 2100

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... NarI(2111) .......... .......... .......... ..........

2101 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg 2160

2101 tttcttggcc cgcggggacg cgactgtcgg ccttgtgccg ccgtagtctc gtcggctaac 2160

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... EagI(2203) ..........

2161 tcagttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt 2220

2161 agtcaacacg ggtcagtatc ggcttatcgg agaggtgggt tcgccggcct cttggacgca 2220

.......... ... ORF 2 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcaatccatc ttgttcaatc atactcttcc tttttcaata ttattgaagc atttatcagg 2280

2221 cgttaggtag aacaagttag tatgagaagg aaaaagttat aataacttcg taaatagtcc 2280

Neomycin(variant) ORF 2 .. .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gttattgtct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 2340

2281 caataacaga gtactggttt tagggaattg cactcaaaag caaggtgact cgcagtctgg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 2400

2341 ggcatctttt ctagtttcct agaagaactc taggaaaaaa agacgcgcat tagacgacga 2400

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2401 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 2460

2401 acgtttgttt ttttggtggc gatggtcgcc accaaacaaa cggcctagtt ctcgatggtt 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 2520

2461 gagaaaaagg cttccattga ccgaagtcgt ctcgcgtcta tggtttatga caggaagatc 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 2580

2521 acatcggcat caatccggtg gtgaagttct tgagacatcg tggcggatgt atggagcgag 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 2640

2581 acgattagga caatggtcac cgacgacggt caccgctatt cagcacagaa tggcccaacc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 2700

2641 tgagttctgc tatcaatggc ctattccgcg tcgccagccc gacttgcccc ccaagcacgt 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 2760

2701 gtgtcgggtc gaacctcgct tgctggatgt ggcttgactc tatggatgtc gcactcgata 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 2820

2761 ctctttcgcg gtgcgaaggg cttccctctt tccgcctgtc cataggccat tcgccgtccc 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 2880

2821 agccttgtcc tctcgcgtgc tccctcgaag gtcccccttt gcggaccata gaaatatcag 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 2940

2881 gacagcccaa agcggtggag actgaactcg cagctaaaaa cactacgagc agtccccccg 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 3000

2941 cctcggatac ctttttgcgg tcgttgcgcc ggaaaaatgc caaggaccgg aaaacgaccg 3000

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 3060

3001 gaaaacgagt gtacaagaaa ggacgcaata ggggactaag acacctattg gcataatggc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 3120

3061 ggaaactcac tcgactatgg cgagcggcgt cggcttgctg gctcgcgtcg ctcagtcact 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 3180

3121 cgctccttcg ccttctcgcg gactacgcca taaaagagga atgcgtagac acgccataaa 3180

.......... .......... .......... .......... .......... ..........

NdeI(3189) .......... .......... .......... .......... ..........

3181 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt 3240

3181 gtgtggcgta taccacgtga gagtcatgtt agacgagact acggcgtatc aattcggtca 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc 3300

3241 tatgtgaggc gatagcgatg cactgaccca gtaccgacgc ggggctgtgg gcggttgtgg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 3360

3301 gcgactgcgc gggactgccc gaacagacga gggccgtagg cgaatgtctg ttcgacactg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca 3420

3361 gcagaggccc tcgacgtaca cagtctccaa aagtggcagt agtggctttg cgcgctccgt 3420

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gcagatcaat tcgcgcgcga aggcgaagcg gcatgcataa tgtgcctgtc aaatggacga 3480

3421 cgtctagtta agcgcgcgct tccgcttcgc cgtacgtatt acacggacag tttacctgct 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agcagggatt ctgcaaaccc tatgctactc cgtcaagccg tcaattgtct gattcgttac 3540

3481 tcgtccctaa gacgtttggg atacgatgag gcagttcggc agttaacaga ctaagcaatg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc 3600

3541 gttaatactg ttgaactgcc gatgtagtaa gtgaaaaaga agtgttggcc gtgccttgag 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa 3660

3601 cgagcccgac cggggccacg taaaaaattt atgggcgctc tttatctcaa ctagcagttt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc 3720

3661 tggttgtaac gctggctgcc accgctatcc gtaggcccac cacgagtttt cgtcgaagcg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa 3780

3721 gaccgactat gcaaccagga gcgcggtcga attctgcgat tagggattga cgaccgcctt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EcoRV(3834)

3781 aagatgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa 3840

3781 ttctacactg tctgcgctgc cgctgttcgt ttgtacgaca cgctgcgacc gctatagttt 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat 3900

3841 taacgacaga cggtccacta gcgactacat gactgttcgg agcgcatggg ctaataggta 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt gctcaagcag 3960

3901 gccacctacc tcgctgagca attagcgaag gtacgcggcg tcattgttaa cgagttcgtc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc 4020

3961 taaatagcgg tcgtcgaggc ttatcgcggg aaggggaacg ggccgcaatt actaaacggg 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc 4080

4021 tttgtccagc gactttacgc cgaccacgcg aagtaggccc gctttcttgg ggcataaccg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca 4140

4081 tttataactg ccggtcaatt cggtaagtac ggtcatccgc gcgcctgctt tcatttgggt 4140

.......... .......... .......... .......... .......... ..........

.......... NruI(4156) .......... .......... .......... ..........

4141 ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg 4200

4141 gaccactatg gtaagcgctc ggaggcctac tgctggcatc actacttaga gaggaccgcc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc 4260

4201 cttgtcgttt tatagtgggc cagccgtttg tttaagagca gggactaaaa agtggtgggg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa 4320

4261 gactggcgct taccactcta actcttatat tggaaagtaa gggtcgccag ccagctattt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa 4380

4321 ttttagctct attggcaacc ggagttagcc gcaatttggg cggtggtcta cccgtaattt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc 4440

4381 gctcataggg ccgtcgtccc ctagtaaaac gcgaagtcgg tatgaaaagt atgagggcgg 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 attcagag.. .......... .......... .......... .......... .......... 4448

4441 taagtctc.. .......... .......... .......... .......... .......... 4448

.......... .......... .......... .......... .......... ..........