GGLOCUS pBAD24 4542 bp DNA SYN 28-Aug-2007 DEFINITION pBAD24 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4542 /organism="pBAD24" /mol_type="other DNA" misc_feature complement(96..974) /label="AraC" CDS complement(96..1025) /label="ORF frame 3" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 1004..1019 /label="araO2" misc_feature 1161..1172 /label="araO1" misc_feature 1203..1216 /label="CAP_BS" misc_feature 1208..1227 /label="pBAD_fwd_primer" misc_feature 1213..1251 /label="AraI1I2" promoter 1248..1276 /label="ARA_promoter" misc_feature complement(1399..1416) /label="pBAD_rev_primer" misc_feature complement(1399..1416) /label="pTrcHis_rev_primer" terminator 1449..1606 /label="rrnB_terminator" terminator 1572..1615 /label="rrnB_T1_terminator" terminator 1747..1774 /label="rrnB_T2_terminator" promoter 1815..1843 /label="AmpR_promoter" gene 1885..2745 /label="Ampicillin(variant)" /gene="Ampicillin(variant)" rep_origin 2804..3103 /label="f1_origin" rep_origin 3337..3956 /label="pBR322_origin" misc_feature 4278..4300 /label="pGEX_3_primer" misc_feature 4439..4458 /label="pBRrevBam_primer" ORIGIN 1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaagag gaattcacca 1321 tggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg 1381 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa 1441 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga 1501 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg 1561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1621 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg 1681 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa 1741 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta 1801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 1861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 1921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 1981 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 2041 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 2101 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc 2161 atacactatt ctcagaatga cttggttgag tgcaacgttg ttgccattgc tacaggcatc 2221 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 2281 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 2341 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 2401 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagtg caaactatta 2461 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 2521 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 2581 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 2641 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 2701 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 2761 tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg 2821 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 2881 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 2941 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 3001 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 3061 gttggagtcc acgttcttta atagtggact cttgttccaa acttgaacaa cactcaaccc 3121 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 3181 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 3241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 3481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 3961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 4021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 4081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt 4141 ttctccttac gcatctgtgc ggtatttcac accgcatagg gtcatggctg cgccccgaca 4201 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 4261 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 4321 acgcgcgagg cagcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca 4381 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg 4441 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga 4501 tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tc //pBAD24 "Times New RomanGC`AraC"Times New RomanGC`ORF 3"Times New RomanGCɆaraO2"Times New RomanGaraO1"Times New RomanGCCAP_BS"Times New RomanGC pBAD_fwd_pri"Times New RomanGCɆAraI1I2"Times New Roman*GARA_pro"Times New RomanGCw pBAD_rev_pri"Times New RomanGCwpTrcHis_rev_pri"Times New RomanGCɆF rrnB_term"Times New RomanG$O rrnB_T1_term"Times New RomanGC rrnB_T2_term"Times New RomanGC3AmpR_pro"Times New RomanGCɆ] Ampicillin(variant)"Times New RomanG   f1_origin"Times New RomanGC t pBR322_origin"Times New RomanGC pGEX_3_pri"Times New RomanGCɆWj pBRrevBam_pri"Times New Roman GClaI"Times New RomanG3 GAflII(301),EcoRV(386)"Times New Roman-YF;V; GNruI"Times New Roman]n~~ GAgeI"Times New Roman2 GfNheI(1300),EcoRI(1311),NcoI(1318),KpnI(1326),SmaI(1328),XbaI(1337),SalI(1343),PstI(1353),HindIII(1361)"Times New Roman<`ei& GAseI"Times New Roman [UO///o> Gtest"Times New RomanEtDbBbgZ Gtest"Times New Romanfw$x