Sequence of pBAD24

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pBAD24

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pBAD24 : 4542 BP; 1083 A; 1191 C; 1135 G; 1133 T; 0 other;

ClaI(2) .......... .......... .......... .......... ..........

1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 60

1 tagctacgta ttacacggac agtttacctg cttcgtccct aagacgtttg ggatacgatg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 120

61 aggcagttcg gcagttaaca gactaagcaa tggttaatac tgttgaactg ccgatgtagt 120

.......... .......... .......... .......... AraC ORF 3 ..........

.......... .......... .......... .......... .......... ..........

121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 180

121 aagtgaaaaa gaagtgttgg ccgtgccttg agcgagcccg accggggcca cgtaaaaaat 180

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 240

181 ttatgggcgc tctttatctc aactagcagt tttggttgta acgctggctg ccaccgctat 240

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 300

241 ccgtaggccc accacgagtt ttcgtcgaag cggaccgact atgcaaccag gagcgcggtc 300

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 360

301 gaattctgcg attagggatt gacgaccgcc ttttctacac tgtctgcgct gccgctgttc 360

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... EcoRV(386) .......... .......... ..........

361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 420

361 gtttgtacga cacgctgcga ccgctatagt tttaacgaca gacggtccac tagcgactac 420

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 480

421 atgactgttc ggagcgcatg ggctaatagg tagccaccta cctcgctgag caattagcga 480

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 540

481 aggtacgcgg cgtcattgtt aacgagttcg tctaaatagc ggtcgtcgag gcttatcgcg 540

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 600

541 ggaaggggaa cgggccgcaa ttactaaacg ggtttgtcca gcgactttac gccgaccacg 600

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 660

601 cgaagtaggc ccgctttctt ggggcataac cgtttataac tgccggtcaa ttcggtaagt 660

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... NruI(708) ..........

661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 720

661 acggtcatcc gcgcgcctgc tttcatttgg gtgaccacta tggtaagcgc tcggaggcct 720

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 780

721 actgctggca tcactactta gagaggaccg cccttgtcgt tttatagtgg gccagccgtt 780

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 840

781 tgtttaagag cagggactaa aaagtggtgg gggactggcg cttaccactc taactcttat 840

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 900

841 attggaaagt aagggtcgcc agccagctat ttttttagct ctattggcaa ccggagttag 900

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 960

901 ccgcaatttg ggcggtggtc tacccgtaat ttgctcatag ggccgtcgtc ccctagtaaa 960

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 1020

961 acgcgaagtc ggtatgaaaa gtatgagggc ggtaagtctc ttctttggtt aacaggtata 1020

AraC .......... ORF 3 .......... .......... araO2

.......... .......... .......... .......... .......... AgeI(1074)

1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 1080

1021 acgtagtctg taacggcagt gacgcagaaa atgaccgaga agagcgattg gtttggccat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 1140

1081 tggggcgaat aattttcgta agacattgtt tcgccctggt ttcggtactg tttttgcgca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 1200

1141 ttgttttcac agatattagt gccgtctttt caggtgtaac taataaacgt gccgcagtgt 1200

.......... .......... araO1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 1260

1201 gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc taggatggac tgcgaaaaat 1260

CAP_BS pBAD_fwd_pri ........ AraI1I2 .......... ARA_pro

.......... .......... .......... NheI(1300) ......... EcoRI(1311)

1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcaagag gaattcacca 1320

1261 agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc gatcgttctc cttaagtggt 1320

ARA_pro .......... .......... .......... .......... ..........

KpnI,SmaI XbaI SalI1343) PstI(1353) HindIII(1361)

1321 tggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggct gttttggcgg 1380

1321 accatgggcc cctaggagat ctcagctgga cgtccgtacg ttcgaaccga caaaaccgcc 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atgagagaag attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa 1440

1381 tactctcttc taaaagtcgg actatgtcta atttagtctt gcgtcttcgc cagactattt 1440

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

1441 acagaatttg cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga 1500

1441 tgtcttaaac ggaccgccgt catcgcgcca ccagggtgga ctggggtacg gcttgagtct 1500

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg 1560

1501 tcactttgcg gcatcgcggc taccatcaca ccccagaggg gtacgctctc atcccttgac 1560

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt 1620

1561 ggtccgtagt ttattttgct ttccgagtca gctttctgac ccggaaagca aaatagacaa 1620

.......... ......... rrnB_term rrnB_T1_term ......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg 1680

1621 caaacagcca cttgcgagag gactcatcct gtttaggcgg ccctcgccta aacttgcaac 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa 1740

1681 gcttcgttgc cgggcctccc accgcccgtc ctgcgggcgg tatttgacgg tccgtagttt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttaagcagaa ggccatcctg acggatggcc tttttgcgtt tctacaaact cttttgttta 1800

1741 aattcgtctt ccggtaggac tgcctaccgg aaaaacgcaa agatgtttga gaaaacaaat 1800

......... rrnB_T2_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt 1860

1801 aaaaagattt atgtaagttt atacataggc gagtactctg ttattgggac tatttacgaa 1860

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc 1920

1861 gttattataa ctttttcctt ctcatactca taagttgtaa aggcacagcg ggaataaggg 1920

.......... .......... .......... ..... Ampicillin(variant) .....

.......... .......... .......... .......... .......... ..........

1921 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa 1980

1921 aaaaaacgcc gtaaaacgga aggacaaaaa cgagtgggtc tttgcgacca ctttcatttt 1980

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt 2040

1981 ctacgacttc tagtcaaccc acgtgctcac ccaatgtagc ttgacctaga gttgtcgcca 2040

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt 2100

2041 ttctaggaac tctcaaaagc ggggcttctt gcaaaaggtt actactcgtg aaaatttcaa 2100

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc 2160

2101 gacgatacac cgcgccataa tagggcacaa ctgcggcccg ttctcgttga gccagcggcg 2160

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 atacactatt ctcagaatga cttggttgag tgcaacgttg ttgccattgc tacaggcatc 2220

2161 tatgtgataa gagtcttact gaaccaactc acgttgcaac aacggtaacg atgtccgtag 2220

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 2280

2221 caccacagtg cgagcagcaa accataccga agtaagtcga ggccaagggt tgctagttcc 2280

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 2340

2281 gctcaatgta ctagggggta caacacgttt tttcgccaat cgaggaagcc aggaggctag 2340

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 2400

2341 caacagtctt cattcaaccg gcgtcacaat agtgagtacc aataccgtcg tgacgtatta 2400

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagtg caaactatta 2460

2401 agagaatgac agtacggtag gcattctacg aaaagacact gaccactcac gtttgataat 2460

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... AseI(2497) .......... ..........

2461 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat 2520

2461 tgaccgcttg atgaatgaga tcgaagggcc gttgttaatt atctgaccta cctccgccta 2520

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa 2580

2521 tttcaacgtc ctggtgaaga cgcgagccgg gaaggccgac cgaccaaata acgactattt 2580

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag 2640

2581 agacctcggc cactcgcacc cagagcgcca tagtaacgtc gtgaccccgg tctaccattc 2640

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat 2700

2641 gggagggcat agcatcaata gatgtgctgc ccctcagtcc gttgatacct acttgcttta 2700

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt 2760

2701 tctgtctagc gactctatcc acggagtgac taattcgtaa ccattgacag tctggttcaa 2760

.......... ..... Ampicillin(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tactcatata tactttagat tgatttacgc gccctgtagc ggcgcattaa gcgcggcggg 2820

2761 atgagtatat atgaaatcta actaaatgcg cgggacatcg ccgcgtaatt cgcgccgccc 2820

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

2821 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 2880

2821 acaccaccaa tgcgcgtcgc actggcgatg tgaacggtcg cgggatcgcg ggcgaggaaa 2880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 2940

2881 gcgaaagaag ggaaggaaag agcggtgcaa gcggccgaaa ggggcagttc gagatttagc 2940

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 3000

2941 ccccgaggga aatcccaagg ctaaatcacg aaatgccgtg gagctggggt tttttgaact 3000

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 3060

3001 aaacccacta ccaagtgcat cacccggtag cgggactatc tgccaaaaag cgggaaactg 3060

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gttggagtcc acgttcttta atagtggact cttgttccaa acttgaacaa cactcaaccc 3120

3061 caacctcagg tgcaagaaat tatcacctga gaacaaggtt tgaacttgtt gtgagttggg 3120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tatctcgggc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 3180

3121 atagagcccg ataagaaaac taaatattcc ctaaaacggc taaagccgga taaccaattt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat 3240

3181 tttactcgac taaattgttt ttaaattgcg cttaaaattg ttttataatt gcaaatgtta 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 3300

3241 aattttccta gatccacttc taggaaaaac tattagagta ctggttttag ggaattgcac 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 3360

3301 tcaaaagcaa ggtgactcgc agtctggggc atcttttcta gtttcctaga agaactctag 3360

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3361 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 3420

3361 gaaaaaaaga cgcgcattag acgacgaacg tttgtttttt tggtggcgat ggtcgccacc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 3480

3421 aaacaaacgg cctagttctc gatggttgag aaaaaggctt ccattgaccg aagtcgtctc 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact 3540

3481 gcgtctatgg tttatgacag gaagatcaca tcggcatcaa tccggtggtg aagttcttga 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 3600

3541 gacatcgtgg cggatgtatg gagcgagacg attaggacaa tggtcaccga cgacggtcac 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 3660

3601 cgctattcag cacagaatgg cccaacctga gttctgctat caatggccta ttccgcgtcg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 3720

3661 ccagcccgac ttgcccccca agcacgtgtg tcgggtcgaa cctcgcttgc tggatgtggc 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 3780

3721 ttgactctat ggatgtcgca ctcgatactc tttcgcggtg cgaagggctt ccctctttcc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 3840

3781 gcctgtccat aggccattcg ccgtcccagc cttgtcctct cgcgtgctcc ctcgaaggtc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 3900

3841 cccctttgcg gaccatagaa atatcaggac agcccaaagc ggtggagact gaactcgcag 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 3960

3901 ctaaaaacac tacgagcagt ccccccgcct cggatacctt tttgcggtcg ttgcgccgga 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 4020

3961 aaaatgccaa ggaccggaaa acgaccggaa aacgagtgta caagaaagga cgcaataggg 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 4080

4021 gactaagaca cctattggca taatggcgga aactcactcg actatggcga gcggcgtcgg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt 4140

4081 cttgctggct cgcgtcgctc agtcactcgc tccttcgcct tctcgcggac tacgccataa 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttctccttac gcatctgtgc ggtatttcac accgcatagg gtcatggctg cgccccgaca 4200

4141 aagaggaatg cgtagacacg ccataaagtg tggcgtatcc cagtaccgac gcggggctgt 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 4260

4201 gggcggttgt gggcgactgc gcgggactgc ccgaacagac gagggccgta ggcgaatgtc 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 4320

4261 tgttcgacac tggcagaggc cctcgacgta cacagtctcc aaaagtggca gtagtggctt 4320

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 acgcgcgagg cagcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca 4380

4321 tgcgcgctcc gtcgttcctc taccgcgggt tgtcaggggg ccggtgcccc ggacggtggt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg 4440

4381 atgggtgcgg ctttgttcgc gagtactcgg gcttcaccgc tcgggctaga aggggtagcc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga 4500

4441 actacagccg ctatatccgc ggtcgttggc gtggacaccg cggccactac ggccggtgct 4500

pBRrevBam_pri ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tgcgtccggc gtagaggatc tgctcatgtt tgacagctta tc........ .......... 4542

4501 acgcaggccg catctcctag acgagtacaa actgtcgaat ag........ .......... 4542

.......... .......... .......... .......... .......... ..........