Sequence of pBAD28

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Sequence of pBAD28

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Sequence of pBAD28 : 5801 BP; 1475 A; 1466 C; 1383 G; 1477 T; 0 other;

ClaI(2) .......... .......... .......... .......... ..........

1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 60

1 tagctacgta ttacacggac agtttacctg cttcgtccct aagacgtttg ggatacgatg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 120

61 aggcagttcg gcagttaaca gactaagcaa tggttaatac tgttgaactg ccgatgtagt 120

.......... .......... .......... .......... AraC ORF 3 ..........

.......... .......... .......... .......... .......... ..........

121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 180

121 aagtgaaaaa gaagtgttgg ccgtgccttg agcgagcccg accggggcca cgtaaaaaat 180

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 240

181 ttatgggcgc tctttatctc aactagcagt tttggttgta acgctggctg ccaccgctat 240

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 300

241 ccgtaggccc accacgagtt ttcgtcgaag cggaccgact atgcaaccag gagcgcggtc 300

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 360

301 gaattctgcg attagggatt gacgaccgcc ttttctacac tgtctgcgct gccgctgttc 360

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... EcoRV(386) .......... .......... ..........

361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 420

361 gtttgtacga cacgctgcga ccgctatagt tttaacgaca gacggtccac tagcgactac 420

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 480

421 atgactgttc ggagcgcatg ggctaatagg tagccaccta cctcgctgag caattagcga 480

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 540

481 aggtacgcgg cgtcattgtt aacgagttcg tctaaatagc ggtcgtcgag gcttatcgcg 540

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 600

541 ggaaggggaa cgggccgcaa ttactaaacg ggtttgtcca gcgactttac gccgaccacg 600

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 660

601 cgaagtaggc ccgctttctt ggggcataac cgtttataac tgccggtcaa ttcggtaagt 660

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... NruI(708) ..........

661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 720

661 acggtcatcc gcgcgcctgc tttcatttgg gtgaccacta tggtaagcgc tcggaggcct 720

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 780

721 actgctggca tcactactta gagaggaccg cccttgtcgt tttatagtgg gccagccgtt 780

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 840

781 tgtttaagag cagggactaa aaagtggtgg gggactggcg cttaccactc taactcttat 840

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 900

841 attggaaagt aagggtcgcc agccagctat ttttttagct ctattggcaa ccggagttag 900

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 960

901 ccgcaatttg ggcggtggtc tacccgtaat ttgctcatag ggccgtcgtc ccctagtaaa 960

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 1020

961 acgcgaagtc ggtatgaaaa gtatgagggc ggtaagtctc ttctttggtt aacaggtata 1020

AraC .......... ORF 3 .......... .......... araO2

.......... .......... .......... .......... .......... ..........

1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 1080

1021 acgtagtctg taacggcagt gacgcagaaa atgaccgaga agagcgattg gtttggccat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 1140

1081 tggggcgaat aattttcgta agacattgtt tcgccctggt ttcggtactg tttttgcgca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 1200

1141 ttgttttcac agatattagt gccgtctttt caggtgtaac taataaacgt gccgcagtgt 1200

.......... .......... araO1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 1260

1201 gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc taggatggac tgcgaaaaat 1260

CAP_BS pBAD_fwd_pri ........ AraI1I2 .......... ARA_pro

.......... .......... .......... .......... .......... ..........

1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt 1320

1261 agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc gatcgcttaa gctcgagcca 1320

ARA_pro .......... .......... .......... .......... ..........

KpnI,SmaI324) XbaI(1333) SalI(1339) HindIII(1357) ..

1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga 1380

1321 tgggccccta ggagatctca gctggacgtc cgtacgttcg aaccgacaaa accgcctact 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag 1440

1381 ctcttctaaa agtcggacta tgtctaattt agtcttgcgt cttcgccaga ctattttgtc 1440

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg 1500

1441 ttaaacggac cgccgtcatc gcgccaccag ggtggactgg ggtacggctt gagtcttcac 1500

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag 1560

1501 tttgcggcat cgcggctacc atcacacccc agaggggtac gctctcatcc cttgacggtc 1560

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1620

1561 cgtagtttat tttgctttcc gagtcagctt tctgacccgg aaagcaaaat agacaacaaa 1620

.......... .... rrnB_term rrnB_T1_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa 1680

1621 cagccacttg cgagaggact catcctgttt aggcggccct cgcctaaact tgcaacgctt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa 1740

1681 cgttgccggg cctcccaccg cccgtcctgc gggcggtatt tgacggtccg tagtttaatt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt 1800

1741 cgtcttccgg taggactgcc taccggaaaa acgcaaagat gtttgagaaa acaaataaaa 1800

......... rrnB_T2_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1860

1801 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1860

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1920

1861 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1920

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1980

1921 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

......... ApaLI(1996) ......... .......... .......... ..........

1981 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 2040

1981 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 2040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2100

2041 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 2160

2101 atacaccgcg ccataatagg gcacaactgc ggcccgttct cgttgagcca gcggcgtatg 2160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2220

2161 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2220

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2280

2221 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2280

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2340

2281 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2340

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2400

2341 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2400

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... FspI(2445) ..........

2401 acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg 2460

2401 tgctcgcact gtggtgctac ggacgtcgtt accgttgttg caacgcgttt gataattgac 2460

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2520

2461 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 2520

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2580

2521 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2580

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2640

2581 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2640

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 2700

2641 gggcatagca tcaatagatg tgctgcccct cagtccgttg atacctactt gctttatctg 2700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2760

2701 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 catatatact ttagattgat ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 2820

2761 gtatatatga aatctaacta aatgcgcggg acatcgccgc gtaattcgcg ccgcccacac 2820

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

2821 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 2880

2821 caccaatgcg cgtcgcactg gcgatgtgaa cggtcgcggg atcgcgggcg aggaaagcga 2880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 2940

2881 aagaagggaa ggaaagagcg gtgcaagcgg ccgaaagggg cagttcgaga tttagccccc 2940

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg 3000

2941 gagggaaatc ccaaggctaa atcacgaaat gccgtggagc tggggttttt tgaactaaac 3000

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 3060

3001 ccactaccaa gtgcatcacc cggtagcggg actatctgcc aaaaagcggg aaactgcaac 3060

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gagtccacgt tctttaatag tggactcttg ttccaaactt gaacaacact caaccctatc 3120

3061 ctcaggtgca agaaattatc acctgagaac aaggtttgaa cttgttgtga gttgggatag 3120

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcgggctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 3180

3121 agcccgataa gaaaactaaa tattccctaa aacggctaaa gccggataac caatttttta 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttaa 3240

3181 ctcgactaaa ttgtttttaa attgcgctta aaattgtttt ataattgcaa atgttaaatt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 3300

3241 ttcctagatc cacttctagg aaaaactatt agagtactgg ttttagggaa ttgcactcaa 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 3360

3301 aagcaaggtg actcgcagtc tggggcatct tttctagttt cctagaagaa ctctaggaaa 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 3420

3361 aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc gccaccaaac 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 3480

3421 aaacggccta gttctcgatg gttgagaaaa aggcttccat tgaccgaagt cgtctcgcgt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 3540

3481 ctatggttta tgacaggaag atcacatcgg catcaatccg gtggtgaagt tcttgagaca 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtcaggcatt tgagaagcac 3600

3541 tcgtggcgga tgtatggagc gagacgatta ggacaatggt cagtccgtaa actcttcgtg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acggtcacac tgcttccggt agtcaataaa ccggtaaacc agcaatagac ataagcggct 3660

3601 tgccagtgtg acgaaggcca tcagttattt ggccatttgg tcgttatctg tattcgccga 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 atttaacgac cctgccctga accgacgacc gggtcgaatt tgctttcgaa tttctgccat 3720

3661 taaattgctg ggacgggact tggctgctgg cccagcttaa acgaaagctt aaagacggta 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcatccgctt attatcactt attcaggcgt agcaccaggc gtttaagggc accaataact 3780

3721 agtaggcgaa taatagtgaa taagtccgca tcgtggtccg caaattcccg tggttattga 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag 3840

3781 cggaattttt ttaatgcggg gcgggacggt gagtagcgtc atgacaacat taagtaattc 3840

.......... .......... ........ ORF 1 CAT/CamR ........ ..........

.......... .......... .......... .......... .......... ..........

3841 cattctgccg acatggaagc catcacagac ggcatgatga acctgaatcg ccagcggcat 3900

3841 gtaagacggc tgtaccttcg gtagtgtctg ccgtactact tggacttagc ggtcgccgta 3900

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt 3960

3901 gtcgtggaac agcggaacgc atattataaa cgggtaccac ttttgccccc gcttcttcaa 3960

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... MscI(3971) .......... .......... .......... ..........

3961 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac 4020

3961 caggtataac cggtgcaaat ttagttttga ccactttgag tgggtcccta accgactctg 4020

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc 4080

4021 ctttttgtat aagagttatt tgggaaatcc ctttatccgg tccaaaagtg gcattgtgcg 4080

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag 4140

4081 gtgtagaacg cttatataca catctttgac ggcctttagc agcaccataa gtgaggtctc 4140

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca 4200

4141 gctacttttg caaagtcaaa cgagtacctt ttgccacatt gttcccactt gtgatagggt 4200

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg 4260

4201 atagtggtcg agtggcagaa agtaacggta tgccttaagg cctactcgta agtagtccgc 4260

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa 4320

4261 ccgttcttac acttatttcc ggcctatttt gaacacgaat aaaaagaaat gccagaaatt 4320

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa 4380

4321 tttccggcat tataggtcga cttgccagac caatatccat gtaactcgtt gactgacttt 4380

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat 4440

4381 acggagtttt acaagaaatg ctacggtaac cctatatagt tgccaccata taggtcacta 4440

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc 4500

4441 aaaaaagagg taaaatcgaa ggaatcgagg acttttagag ctattgagtt ttttatgcgg 4500

ORF 1 CAT/CamR ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc 4560

4501 gccatcacta gaataaagta ataccacttt caaccttgga gaatgcacgg ctagttgcag 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac caggatttat 4620

4561 agtaaaagcg gttttcaacc gggtcccgaa gggccatagt tgtccctgtg gtcctaaata 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttattctgcg aagtgatctt ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga 4680

4621 aataagacgc ttcactagaa ggcagtgtcc ataaataagc cgcgtttcac gcagcccact 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgctgccaac ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg 4740

4681 acgacggttg aatgactaaa tcacatacta ccacaaaaac tccacgaggt caccgaagac 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca 4800

4741 aaagatagtc gacagggagg acaagtcgat gactgcccca ccacgcattg ccgttttcgt 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccgccggaca tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg 4860

4801 ggcggcctgt agtcgcgatc gcctcacata tgaccgaatg atacaaccgt gactactccc 4860

.......... .......... ......... p15A_origin ......... ..........

.......... .......... .......... .......... .......... ..........

4861 tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat 4920

4861 acagtcactt cacgaagtac accgtcctct tttttccgac gtggccacgc agtcgtctta 4920

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga 4980

4921 tacactatgt cctatataag gcgaaggagc gagtgactga gcgatgcgag ccagcaagct 4980

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag 5040

4981 gacgccgctc gcctttaccg aatgcttgcc ccgcctctaa aggaccttct acggtccttc 5040

.......... ......... p15A_origin ......... .......... ..........

.......... ......... SacII(5068) ......... .......... ..........

5041 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc 5100

5041 tatgaattgt cccttcactc tcccggcgcc gtttcggcaa aaaggtatcc gaggcggggg 5100

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat 5160

5101 gactgttcgt agtgctttag actgcgagtt tagtcaccac cgctttgggc tgtcctgata 5160

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc 5220

5161 tttctatggt ccgcaaaggg ggaccgccga gggagcacgc gagaggacaa ggacggaaag 5220

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc 5280

5221 ccaaatggcc acagtaaggc gacaataccg gcgcaaacag agtaaggtgc ggactgtgag 5280

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc 5340

5281 tcaaggccca tccgtcaagc gaggttcgac ctgacatacg tgcttggggg gcaagtcagg 5340

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa 5400

5341 ctggcgacgc ggaataggcc attgatagca gaactcaggt tgggcctttc tgtacgtttt 5400

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc 5460

5401 cgtggtgacc gtcgtcggtg accattaact aaatctcctc aatcagaact tcagtacgcg 5460

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc 5520

5461 gccaattccg atttgacttt cctgttcaaa accactgacg cgaggaggtt cggtcaatgg 5520

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt 5580

5521 agccaagttt ctcaaccatc gagtctcttg gaagcttttt ggcgggacgt tccgccaaaa 5580

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat 5640

5581 aagcaaaagt ctcgttctct aatgcgcgtc tggttttgct agagttcttc tagtagaata 5640

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 taatcagata aaatatttgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt 5700

5641 attagtctat tttataaacg agtactcggg cttcaccgct cgggctagaa ggggtagcca 5700

p15A_origin ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat 5760

5701 ctacagccgc tatatccgcg gtcgttggcg tggacaccgc ggccactacg gccggtgcta 5760

pBRrevBam_pri ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gcgtccggcg tagaggatct gctcatgttt gacagcttat c......... .......... 5801

5761 cgcaggccgc atctcctaga cgagtacaaa ctgtcgaata g......... .......... 5801

.......... .......... .......... .......... .......... ..........