Sequence of pBAD33

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Sequence of pBAD33

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Sequence of pBAD33 : 5352 BP; 1351 A; 1357 C; 1269 G; 1375 T; 0 other;

ClaI(2) .......... .......... .......... .......... ..........

1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 60

1 tagctacgta ttacacggac agtttacctg cttcgtccct aagacgtttg ggatacgatg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 120

61 aggcagttcg gcagttaaca gactaagcaa tggttaatac tgttgaactg ccgatgtagt 120

.......... .......... .......... .......... AraC ORF 3 ..........

.......... .......... .......... .......... .......... ..........

121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 180

121 aagtgaaaaa gaagtgttgg ccgtgccttg agcgagcccg accggggcca cgtaaaaaat 180

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 240

181 ttatgggcgc tctttatctc aactagcagt tttggttgta acgctggctg ccaccgctat 240

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 300

241 ccgtaggccc accacgagtt ttcgtcgaag cggaccgact atgcaaccag gagcgcggtc 300

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 360

301 gaattctgcg attagggatt gacgaccgcc ttttctacac tgtctgcgct gccgctgttc 360

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... EcoRV(386) .......... .......... ..........

361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 420

361 gtttgtacga cacgctgcga ccgctatagt tttaacgaca gacggtccac tagcgactac 420

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 480

421 atgactgttc ggagcgcatg ggctaatagg tagccaccta cctcgctgag caattagcga 480

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 540

481 aggtacgcgg cgtcattgtt aacgagttcg tctaaatagc ggtcgtcgag gcttatcgcg 540

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 600

541 ggaaggggaa cgggccgcaa ttactaaacg ggtttgtcca gcgactttac gccgaccacg 600

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 660

601 cgaagtaggc ccgctttctt ggggcataac cgtttataac tgccggtcaa ttcggtaagt 660

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... NruI(708) ..........

661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 720

661 acggtcatcc gcgcgcctgc tttcatttgg gtgaccacta tggtaagcgc tcggaggcct 720

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 780

721 actgctggca tcactactta gagaggaccg cccttgtcgt tttatagtgg gccagccgtt 780

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 840

781 tgtttaagag cagggactaa aaagtggtgg gggactggcg cttaccactc taactcttat 840

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 900

841 attggaaagt aagggtcgcc agccagctat ttttttagct ctattggcaa ccggagttag 900

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 960

901 ccgcaatttg ggcggtggtc tacccgtaat ttgctcatag ggccgtcgtc ccctagtaaa 960

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 1020

961 acgcgaagtc ggtatgaaaa gtatgagggc ggtaagtctc ttctttggtt aacaggtata 1020

AraC .......... ORF 3 .......... .......... araO2

.......... .......... .......... .......... .......... ..........

1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 1080

1021 acgtagtctg taacggcagt gacgcagaaa atgaccgaga agagcgattg gtttggccat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 1140

1081 tggggcgaat aattttcgta agacattgtt tcgccctggt ttcggtactg tttttgcgca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 1200

1141 ttgttttcac agatattagt gccgtctttt caggtgtaac taataaacgt gccgcagtgt 1200

.......... .......... araO1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 1260

1201 gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc taggatggac tgcgaaaaat 1260

CAP_BS pBAD_fwd_pri ........ AraI1I2 .......... ARA_pro

.......... .......... .......... .......... .......... ..........

1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt 1320

1261 agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc gatcgcttaa gctcgagcca 1320

ARA_pro .......... .......... .......... .......... ..........

KpnI,SmaI XbaI SalI1339) PstI(1349) HindIII(1357)

1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga 1380

1321 tgggccccta ggagatctca gctggacgtc cgtacgttcg aaccgacaaa accgcctact 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag 1440

1381 ctcttctaaa agtcggacta tgtctaattt agtcttgcgt cttcgccaga ctattttgtc 1440

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg 1500

1441 ttaaacggac cgccgtcatc gcgccaccag ggtggactgg ggtacggctt gagtcttcac 1500

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag 1560

1501 tttgcggcat cgcggctacc atcacacccc agaggggtac gctctcatcc cttgacggtc 1560

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1620

1561 cgtagtttat tttgctttcc gagtcagctt tctgacccgg aaagcaaaat agacaacaaa 1620

.......... .... rrnB_term rrnB_T1_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa 1680

1621 cagccacttg cgagaggact catcctgttt aggcggccct cgcctaaact tgcaacgctt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa 1740

1681 cgttgccggg cctcccaccg cccgtcctgc gggcggtatt tgacggtccg tagtttaatt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt 1800

1741 cgtcttccgg taggactgcc taccggaaaa acgcaaagat gtttgagaaa acaaataaaa 1800

......... rrnB_T2_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1860

1801 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1860

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1920

1861 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1980

1921 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctgaagatca gttggggcaa actattaact ggcgaactac ttactctagc ttcccggcaa 2040

1981 gacttctagt caaccccgtt tgataattga ccgcttgatg aatgagatcg aagggccgtt 2040

.......... .......... .......... .......... Ampicillin ..........

.......... .......... .......... .......... .......... ..........

2041 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 2100

2041 gttaattatc tgacctacct ccgcctattt caacgtcctg gtgaagacgc gagccgggaa 2100

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 2160

2101 ggccgaccga ccaaataacg actatttaga cctcggccac tcgcacccag agcgccatag 2160

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 2220

2161 taacgtcgtg accccggtct accattcggg agggcatagc atcaatagat gtgctgcccc 2220

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 2280

2221 tcagtccgtt gatacctact tgctttatct gtctagcgac tctatccacg gagtgactaa 2280

.......... .......... Ampicillin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttacgcgcc 2340

2281 ttcgtaacca ttgacagtct ggttcaaatg agtatatatg aaatctaact aaatgcgcgg 2340

Ampicillin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 2400

2341 gacatcgccg cgtaattcgc gccgcccaca ccaccaatgc gcgtcgcact ggcgatgtga 2400

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 2460

2401 acggtcgcgg gatcgcgggc gaggaaagcg aaagaaggga aggaaagagc ggtgcaagcg 2460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt 2520

2461 gccgaaaggg gcagttcgag atttagcccc cgagggaaat cccaaggcta aatcacgaaa 2520

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc 2580

2521 tgccgtggag ctggggtttt ttgaactaaa cccactacca agtgcatcac ccggtagcgg 2580

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 2640

2581 gactatctgc caaaaagcgg gaaactgcaa cctcaggtgc aagaaattat cacctgagaa 2640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gttccaaact tgaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat 2700

2641 caaggtttga acttgttgtg agttgggata gagcccgata agaaaactaa atattcccta 2700

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 2760

2701 aaacggctaa agccggataa ccaatttttt actcgactaa attgttttta aattgcgctt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttttaacaaa atattaacgt ttacaattta aaaggatcta ggtgaagatc ctttttgata 2820

2761 aaaattgttt tataattgca aatgttaaat tttcctagat ccacttctag gaaaaactat 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 2880

2821 tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 2940

2881 ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 3000

2941 gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 3060

3001 aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaggaa gatcacatcg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 3120

3061 gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcctgttacc agtcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa 3180

3121 aggacaatgg tcagtccgta aactcttcgt gtgccagtgt gacgaaggcc atcagttatt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac 3240

3181 tggccatttg gtcgttatct gtattcgccg ataaattgct gggacgggac ttggctgctg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg 3300

3241 gcccagctta aacgaaagct taaagacggt aagtaggcga ataatagtga ataagtccgc 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc 3360

3301 atcgtggtcc gcaaattccc gtggttattg acggaatttt tttaatgcgg ggcgggacgg 3360

.......... .......... .......... .......... ........ ORF 2 CAT/CamR

.......... .......... .......... .......... .......... ..........

3361 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga 3420

3361 tgagtagcgt catgacaaca ttaagtaatt cgtaagacgg ctgtaccttc ggtagtgtct 3420

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt 3480

3421 gccgtactac ttggacttag cggtcgccgt agtcgtggaa cagcggaacg catattataa 3480

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

NcoI(3484) .......... .......... .......... MscI(3522) ..........

3481 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac 3540

3481 acgggtacca cttttgcccc cgcttcttca acaggtataa ccggtgcaaa tttagttttg 3540

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag 3600

3541 accactttga gtgggtccct aaccgactct gctttttgta taagagttat ttgggaaatc 3600

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact 3660

3601 cctttatccg gtccaaaagt ggcattgtgc ggtgtagaac gcttatatac acatctttga 3660

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga 3720

3661 cggcctttag cagcaccata agtgaggtct cgctactttt gcaaagtcaa acgagtacct 3720

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca 3780

3721 tttgccacat tgttcccact tgtgataggg tatagtggtc gagtggcaga aagtaacggt 3780

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa 3840

3781 atgccttaag gcctactcgt aagtagtccg cccgttctta cacttatttc cggcctattt 3840

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct 3900

3841 tgaacacgaa taaaaagaaa tgccagaaat ttttccggca ttataggtcg acttgccaga 3900

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt 3960

3901 ccaatatcca tgtaactcgt tgactgactt tacggagttt tacaagaaat gctacggtaa 3960

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc 4020

3961 ccctatatag ttgccaccat ataggtcact aaaaaaagag gtaaaatcga aggaatcgag 4020

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa 4080

4021 gacttttaga gctattgagt tttttatgcg ggccatcact agaataaagt aataccactt 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct 4140

4081 tcaaccttgg agaatgcacg gctagttgca gagtaaaagc ggttttcaac cgggtcccga 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag 4200

4141 agggccatag ttgtccctgt ggtcctaaat aaataagacg cttcactaga aggcagtgtc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt tagtgtatga 4260

4201 cataaataag ccgcgtttca cgcagcccac tacgacggtt gaatgactaa atcacatact 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct 4320

4261 accacaaaaa ctccacgagg tcaccgaaga caaagatagt cgacagggag gacaagtcga 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta gcggagtgta 4380

4321 tgactgcccc accacgcatt gccgttttcg tggcggcctg tagtcgcgat cgcctcacat 4380

.......... .......... .......... .......... ......... p15A_origin

.......... .......... .......... .......... .......... ..........

4381 tactggctta ctatgttggc actgatgagg gtgtcagtga agtgcttcat gtggcaggag 4440

4381 atgaccgaat gatacaaccg tgactactcc cacagtcact tcacgaagta caccgtcctc 4440

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aaaaaaggct gcaccggtgc gtcagcagaa tatgtgatac aggatatatt ccgcttcctc 4500

4441 ttttttccga cgtggccacg cagtcgtctt atacactatg tcctatataa ggcgaaggag 4500

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggaaatgg cttacgaacg 4560

4501 cgagtgactg agcgatgcga gccagcaagc tgacgccgct cgcctttacc gaatgcttgc 4560

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... ......... SacII(4619)

4561 gggcggagat ttcctggaag atgccaggaa gatacttaac agggaagtga gagggccgcg 4620

4561 cccgcctcta aaggaccttc tacggtcctt ctatgaattg tcccttcact ctcccggcgc 4620

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat ctgacgctca 4680

4621 cgtttcggca aaaaggtatc cgaggcgggg ggactgttcg tagtgcttta gactgcgagt 4680

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aatcagtggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggcggc 4740

4681 ttagtcacca ccgctttggg ctgtcctgat atttctatgg tccgcaaagg gggaccgccg 4740

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg 4800

4741 agggagcacg cgagaggaca aggacggaaa gccaaatggc cacagtaagg cgacaatacc 4800

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct 4860

4801 ggcgcaaaca gagtaaggtg cggactgtga gtcaaggccc atccgtcaag cgaggttcga 4860

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc gccttatccg gtaactatcg 4920

4861 cctgacatac gtgcttgggg ggcaagtcag gctggcgacg cggaataggc cattgatagc 4920

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcttgagtcc aacccggaaa gacatgcaaa agcaccactg gcagcagcca ctggtaattg 4980

4921 agaactcagg ttgggccttt ctgtacgttt tcgtggtgac cgtcgtcggt gaccattaac 4980

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 atttagagga gttagtcttg aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt 5040

4981 taaatctcct caatcagaac ttcagtacgc ggccaattcc gatttgactt tcctgttcaa 5040

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttggtgactg cgctcctcca agccagttac ctcggttcaa agagttggta gctcagagaa 5100

5041 aaccactgac gcgaggaggt tcggtcaatg gagccaagtt tctcaaccat cgagtctctt 5100

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ccttcgaaaa accgccctgc aaggcggttt tttcgttttc agagcaagag attacgcgca 5160

5101 ggaagctttt tggcgggacg ttccgccaaa aaagcaaaag tctcgttctc taatgcgcgt 5160

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gaccaaaacg atctcaagaa gatcatctta ttaatcagat aaaatatttg ctcatgagcc 5220

5161 ctggttttgc tagagttctt ctagtagaat aattagtcta ttttataaac gagtactcgg 5220

.......... ......... p15A_origin ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg 5280

5221 gcttcaccgc tcgggctaga aggggtagcc actacagccg ctatatccgc ggtcgttggc 5280

.......... .......... ........ pBRrevBam_pri ........ ..........

.......... .......... .......... .......... .......... ..........

5281 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc tgctcatgtt 5340

5281 gtggacaccg cggccactac ggccggtgct acgcaggccg catctcctag acgagtacaa 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tgacagctta tc........ .......... .......... .......... .......... 5352

5341 actgtcgaat ag........ .......... .......... .......... .......... 5352

.......... .......... .......... .......... .......... ..........