FWFW"LOCUS pBAD40 4806 bp DNA SYN 18-Sep-2007 DEFINITION pBAD40 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4806 /organism="pBAD40" /mol_type="other DNA" misc_feature complement(96..974) /label="AraC" CDS complement(96..1025) /label="ORF frame 3" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 1004..1019 /label="araO2" misc_feature 1161..1172 /label="araO1" misc_feature 1203..1216 /label="CAP_BS" misc_feature 1208..1227 /label="pBAD_fwd_primer" misc_feature 1213..1251 /label="AraI1I2" promoter 1248..1276 /label="ARA_promoter" misc_feature complement(1328..1344) /label="M13_forward20_primer" misc_feature complement(1337..1359) /label="M13_pUC_fwd_primer" misc_feature 1325..1480 /label="lacZ_a" CDS complement(1787..2446) /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 2" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene complement(1787..2446) /label="CAT/CamR" /gene="CAT/CamR" CDS 3317..4267 /label="ORF frame 2" /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI*" misc_feature 4542..4564 /label="pGEX_3_primer" misc_feature 4703..4722 /label="pBRrevBam_primer" ORIGIN 1 ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC 61 TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA 121 TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA 181 AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA 241 GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG 301 CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG 361 CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG 421 TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT 481 TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC 541 CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC 601 GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA 661 TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA 721 TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA 781 ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA 841 TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC 901 GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT 961 TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT 1021 TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA 1081 ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT 1141 AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA 1201 CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG ATCCTACCTG ACGCTTTTTA 1261 TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGAGTCGA CGGATCCCCG 1321 GGAATTCACT GGCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC GTTACCCAAC 1381 TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA GAGGCCCGCA 1441 CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCTAA CCGTTTTTAT 1501 CAGGCTCTGG GAGGCAGAAT AAATGATCAT ATCGTCAATT ATTACCTCCA CGGGGAGAGC 1561 CTGAGCAAAC TGGCCTCAGG CATTTGAGAA GCACACGGTC ACACTGCTTC CGGTAGTCAA 1621 TAAACCGGTA AACCAGCAAT AGACATAAGC GGCTATTTAA CGACCCTGCC CTGAACCGAC 1681 GACCGGGTCG AATTTGCTTT CGAATTTCTG CCATTCATCC GCTTATTATC ACTTATTCAG 1741 GCGTAGCACC AGGCGTTTAA GGGCACCAAT AACTGCCTTA AAAAAATTAC GCCCCGCCCT 1801 GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG AAGCCATCAC 1861 AGACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT TGCGTATAAT 1921 ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG TTTAAATCAA 1981 AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA ATAAACCCTT 2041 TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT ATGTGTAGAA 2101 ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA GTTTGCTCAT 2161 GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG TCTTTCATTG 2221 CCATACGAAA TTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA AAGGCCGGAT 2281 AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC AGCTGAACGG 2341 TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT TTACGATGCC 2401 ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATTTTA GCTTCCTTAG 2461 CTCCTGAAAA TCTCGATAAC TCAAAAAATA CGCCCGGTAG TGATCTTATT TCATTATGGT 2521 GAAAGTTGGA ACCTCTTACG TGCCGATCAA CGTCTCATTT TCGCCAAAAG TTGGCCCAGG 2581 GCTTCCCGGT ATCAACAGGG ACACCAGGAT TTATTTATTC TGCGAAGTGA TCTTCCGTCA 2641 CAGGTATTTA TTCGGCGCAA AGTGCGTCGG GTGATGCTGC CAACTTACTG ATTTAGTGTA 2701 TGATGGTGTT TTTGAGGTGC TCCAGTGGCT TCTGTTTCTA TCAGCTGTCC CTCCTGTTCA 2761 GCTACTGACG GGGTGGTGCG TAACGGCAAA AGCACCGCCG GACATCAGCG CCTGTAGTGC 2821 CATTTACCCC CATTCACTGC CAGAGCCGTG AGCGCAGCGA ACTGAATGTC ACGAAAAAGA 2881 CAGCGACTCA GGTGCCTGAT GGTCGGAGAC AAAAGGAATA TTCAGCGATT TGCCCGAGCT 2941 TGCGAGGGTG CTACTTAAGC CTTTAGGGTT TTAAGGTCTG TTTTGTAGAG GAGCAAACAG 3001 CGTTTGCGAC ATCCTTTTGT AATACTGCGG AACTGACTAA AGTAGTGAGT TATACACAGG 3061 GCTGGGATCT ATTCTTTTTA TCTTTTTTTA TTCTTTCTTT ATTCTATAAA TTATAACCAC 3121 TTGAATATAA ACAAAAAAAA CACACAAAGG TCTAGCGGAA TTTACAGAGG GTCTAGCAGA 3181 ATTTACAAGT TTTCCAGCAA AGGTCTAGCA GAATTTACAG ATACCCACAA CTCAAAGGAA 3241 AAGGACTAGT AATTATCATT GACTAGCCCA TCTCAATTGG TATAGTGATT AAAATCACCT 3301 AGACCAATTG AGATGTATGT CTGAATTAGT TGTTTTCAAA GCAAATGAAC TAGCGATTAG 3361 TCGCTATGAC TTAACGGAGC ATGAAACCAA GCTAATTTTA TGCTGTGTGG CACTACTCAA 3421 CCCCACGATT GAAAACCCTA CAAGGAAAGA ACGGACGGTA TCGTTCACTT ATAACCAATA 3481 CGCTCAGATG ATGAACATCA GTAGGGAAAA TGCTTATGGT GTATTAGCTA AAGCAACCAG 3541 AGAGCTGATG ACGAGAACTG TGGAAATCAG GAATCCTTTG GTTAAAGGCT TTGAGATTTT 3601 CCAGTGGACA AACTATGCCA AGTTCTCAAG CGAAAAATTA GAATTAGTTT TTAGTGAAGA 3661 GATATTGCCT TATCTTTTCC AGTTAAAAAA ATTCATAAAA TATAATCTGG AACATGTTAA 3721 GTCTTTTGAA AACAAATACT CTATGAGGAT TTATGAGTGG TTATTAAAAG AACTAACACA 3781 AAAGAAAACT CACAAGGCAA ATATAGAGAT TAGCCTTGAT GAATTTAAGT TCATGTTAAT 3841 GCTTGAAAAT AACTACCATG AGTTTAAAAG GCTTAACCAA TGGGTTTTGA AACCAATAAG 3901 TAAAGATTTA AACACTTACA GCAATATGAA ATTGGTGGTT GATAAGCGAG GCCGCCCGAC 3961 TGATACGTTG ATTTTCCAAG TTGAACTAGA TAGACAAATG GATCTCGTAA CCGAACTTGA 4021 GAACAACCAG ATAAAAATGA ATGGTGACAA AATACCAACA ACCATTACAT CAGATTCCTA 4081 CCTACGTAAC GGACTAAGAA AAACACTACA CGATGCTTTA ACTGCAAAAA TTCAGCTCAC 4141 CAGTTTTGAG GCAAAATTTT TGAGTGACAT GCAAAGTAAG CATGATCTCA ATGGTTCGTT 4201 CTCATGGCTC ACGCAAAAAC AACGAACCAC ACTAGAGAAC ATACTGGCTA AATACGGAAG 4261 GATCTGAGGT TCTTATGGCT CTTGTATCTA TCAGTGAAGC ATCAAGACTA ACAAACAAAA 4321 GTAGAACAAC TGTTCACCGT TAGATATCAA AGGGAAAACT GTCCATATGG TGCACTCTCA 4381 GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA 4441 CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG 4501 TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA 4561 GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAA GGAGATGGCG CCCAACAGTC 4621 CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT 4681 GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG 4741 TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCTGCTCA TGTTTGACAG 4801 CTTATC //pBAD40 "Times New RomanFWC`AraC"Times New RomanFWC`ORF 3"Times New RomanFWCɆaraO2"Times New RomanFWaraO1"Times New RomanFWCCAP_BS"Times New RomanFWC pBAD_fwd_pri"Times New RomanFWCɆAraI1I2"Times New Roman*FWARA_pro"Times New RomanFWCɆ-lacZ_a"Times New RomanFWC0@M13_forward20_pri"Times New RomanFWC9OM13_pUC_fwd_pri"Times New Roman*FW ORF 2"Times New RomanFWC CAT/CamR"Times New RomanFWC ORF 2"Times New RomanFWCɆ pGEX_3_pri"Times New RomanFW_r pBRrevBam_pri"Times New Roman FWClaI"Times New RomanqFq2q] FWNruI"Times New Roman(8GGy FW!SalI(1306),SmaI(1319),EcoRI(1322)"Times New RomanUHiJ|L|6b FWFspI(1464),BclI(1524)"Times New RomanJv]}oot FWBstBI"Times New Roman(8GG FWNcoI(1927),MscI(1965)"Times New RomanS$ FWSpeI"Times New Roman B FWNdeI(4365),ApaLI(4370)"Times New Roman m\K-K FWtest"Times New Roman)TLGG7E 7