Sequence of pBAD40

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Sequence of pBAD40

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Sequence of pBAD40 : 4806 BP; 1371 A; 1165 C; 1061 G; 1209 T; 0 other;

ClaI(2) .......... .......... .......... .......... ..........

1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 60

1 tagctacgta ttacacggac agtttacctg cttcgtccct aagacgtttg ggatacgatg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 120

61 aggcagttcg gcagttaaca gactaagcaa tggttaatac tgttgaactg ccgatgtagt 120

.......... .......... .......... .......... AraC ORF 3 ..........

.......... .......... .......... .......... .......... ..........

121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 180

121 aagtgaaaaa gaagtgttgg ccgtgccttg agcgagcccg accggggcca cgtaaaaaat 180

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 240

181 ttatgggcgc tctttatctc aactagcagt tttggttgta acgctggctg ccaccgctat 240

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 300

241 ccgtaggccc accacgagtt ttcgtcgaag cggaccgact atgcaaccag gagcgcggtc 300

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 360

301 gaattctgcg attagggatt gacgaccgcc ttttctacac tgtctgcgct gccgctgttc 360

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 420

361 gtttgtacga cacgctgcga ccgctatagt tttaacgaca gacggtccac tagcgactac 420

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 480

421 atgactgttc ggagcgcatg ggctaatagg tagccaccta cctcgctgag caattagcga 480

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 540

481 aggtacgcgg cgtcattgtt aacgagttcg tctaaatagc ggtcgtcgag gcttatcgcg 540

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 600

541 ggaaggggaa cgggccgcaa ttactaaacg ggtttgtcca gcgactttac gccgaccacg 600

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 660

601 cgaagtaggc ccgctttctt ggggcataac cgtttataac tgccggtcaa ttcggtaagt 660

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... NruI(708) ..........

661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 720

661 acggtcatcc gcgcgcctgc tttcatttgg gtgaccacta tggtaagcgc tcggaggcct 720

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 780

721 actgctggca tcactactta gagaggaccg cccttgtcgt tttatagtgg gccagccgtt 780

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 840

781 tgtttaagag cagggactaa aaagtggtgg gggactggcg cttaccactc taactcttat 840

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 900

841 attggaaagt aagggtcgcc agccagctat ttttttagct ctattggcaa ccggagttag 900

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 960

901 ccgcaatttg ggcggtggtc tacccgtaat ttgctcatag ggccgtcgtc ccctagtaaa 960

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 1020

961 acgcgaagtc ggtatgaaaa gtatgagggc ggtaagtctc ttctttggtt aacaggtata 1020

AraC .......... ORF 3 .......... .......... araO2

.......... .......... .......... .......... .......... ..........

1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 1080

1021 acgtagtctg taacggcagt gacgcagaaa atgaccgaga agagcgattg gtttggccat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 1140

1081 tggggcgaat aattttcgta agacattgtt tcgccctggt ttcggtactg tttttgcgca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 1200

1141 ttgttttcac agatattagt gccgtctttt caggtgtaac taataaacgt gccgcagtgt 1200

.......... .......... araO1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 1260

1201 gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc taggatggac tgcgaaaaat 1260

CAP_BS pBAD_fwd_pri ........ AraI1I2 .......... ARA_pro

.......... .......... .......... .......... SalI(1306) ..........

1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagagtcga cggatccccg 1320

1261 agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc gatctcagct gcctaggggc 1320

ARA_pro .......... .......... .......... .......... ..........

EcoRI(1322) ........ .......... .......... .......... ..........

1321 ggaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 1380

1321 ccttaagtga ccggcagcaa aatgttgcag cactgaccct tttgggaccg caatgggttg 1380

... M13_forward20_pri M13_pUC_fwd_pri lacZ_a ... ..........

.......... .......... .......... .......... .......... ..........

1381 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 1440

1381 aattagcgga acgtcgtgta gggggaaagc ggtcgaccgc attatcgctt ctccgggcgt 1440

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgctaa ccgtttttat 1500

1441 ggctagcggg aagggttgtc aacgcgtcgg acttaccgct taccgcgatt ggcaaaaata 1500

.......... lacZ_a .......... .......... .......... ..........

.......... .......... BclI(1524) .......... .......... ..........

1501 caggctctgg gaggcagaat aaatgatcat atcgtcaatt attacctcca cggggagagc 1560

1501 gtccgagacc ctccgtctta tttactagta tagcagttaa taatggaggt gcccctctcg 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctgagcaaac tggcctcagg catttgagaa gcacacggtc acactgcttc cggtagtcaa 1620

1561 gactcgtttg accggagtcc gtaaactctt cgtgtgccag tgtgacgaag gccatcagtt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc ctgaaccgac 1680

1621 atttggccat ttggtcgtta tctgtattcg ccgataaatt gctgggacgg gacttggctg 1680

.......... .......... .......... .......... .......... ..........

......... BstBI(1700) ......... .......... .......... ..........

1681 gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag 1740

1681 ctggcccagc ttaaacgaaa gcttaaagac ggtaagtagg cgaataatag tgaataagtc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcgtagcacc aggcgtttaa gggcaccaat aactgcctta aaaaaattac gccccgccct 1800

1741 cgcatcgtgg tccgcaaatt cccgtggtta ttgacggaat ttttttaatg cggggcggga 1800

.......... .......... .......... .......... ........ ORF 2 CAT/CamR

.......... .......... .......... .......... .......... ..........

1801 gccactcatc gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac 1860

1801 cggtgagtag cgtcatgaca acattaagta attcgtaaga cggctgtacc ttcggtagtg 1860

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agacggcatg atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat 1920

1861 tctgccgtac tacttggact tagcggtcgc cgtagtcgtg gaacagcgga acgcatatta 1920

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

NcoI(1927) .......... .......... .......... MscI(1965) ..........

1921 atttgcccat ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa 1980

1921 taaacgggta ccacttttgc ccccgcttct tcaacaggta taaccggtgc aaatttagtt 1980

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aactggtgaa actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt 2040

1981 ttgaccactt tgagtgggtc cctaaccgac tctgcttttt gtataagagt tatttgggaa 2040

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tagggaaata ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa 2100

2041 atccctttat ccggtccaaa agtggcattg tgcggtgtag aacgcttata tacacatctt 2100

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 actgccggaa atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat 2160

2101 tgacggcctt tagcagcacc ataagtgagg tctcgctact tttgcaaagt caaacgagta 2160

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggaaaacggt gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg 2220

2161 ccttttgcca cattgttccc acttgtgata gggtatagtg gtcgagtggc agaaagtaac 2220

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccatacgaaa ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat 2280

2221 ggtatgcttt aaggcctact cgtaagtagt ccgcccgttc ttacacttat ttccggccta 2280

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaaacttgtg cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg 2340

2281 ttttgaacac gaataaaaag aaatgccaga aatttttccg gcattatagg tcgacttgcc 2340

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tctggttata ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc 2400

2341 agaccaatat ccatgtaact cgttgactga ctttacggag ttttacaaga aatgctacgg 2400

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 attgggatat atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag 2460

2401 taaccctata tagttgccac catataggtc actaaaaaaa gaggtaaaat cgaaggaatc 2460

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt 2520

2461 gaggactttt agagctattg agttttttat gcgggccatc actagaataa agtaatacca 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gaaagttgga acctcttacg tgccgatcaa cgtctcattt tcgccaaaag ttggcccagg 2580

2521 ctttcaacct tggagaatgc acggctagtt gcagagtaaa agcggttttc aaccgggtcc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcttcccggt atcaacaggg acaccaggat ttatttattc tgcgaagtga tcttccgtca 2640

2581 cgaagggcca tagttgtccc tgtggtccta aataaataag acgcttcact agaaggcagt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc caacttactg atttagtgta 2700

2641 gtccataaat aagccgcgtt tcacgcagcc cactacgacg gttgaatgac taaatcacat 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca 2760

2701 actaccacaa aaactccacg aggtcaccga agacaaagat agtcgacagg gaggacaagt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gctactgacg gggtggtgcg taacggcaaa agcaccgccg gacatcagcg cctgtagtgc 2820

2761 cgatgactgc cccaccacgc attgccgttt tcgtggcggc ctgtagtcgc ggacatcacg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 catttacccc cattcactgc cagagccgtg agcgcagcga actgaatgtc acgaaaaaga 2880

2821 gtaaatgggg gtaagtgacg gtctcggcac tcgcgtcgct tgacttacag tgctttttct 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cagcgactca ggtgcctgat ggtcggagac aaaaggaata ttcagcgatt tgcccgagct 2940

2881 gtcgctgagt ccacggacta ccagcctctg ttttccttat aagtcgctaa acgggctcga 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgcgagggtg ctacttaagc ctttagggtt ttaaggtctg ttttgtagag gagcaaacag 3000

2941 acgctcccac gatgaattcg gaaatcccaa aattccagac aaaacatctc ctcgtttgtc 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgtttgcgac atccttttgt aatactgcgg aactgactaa agtagtgagt tatacacagg 3060

3001 gcaaacgctg taggaaaaca ttatgacgcc ttgactgatt tcatcactca atatgtgtcc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gctgggatct attcttttta tcttttttta ttctttcttt attctataaa ttataaccac 3120

3061 cgaccctaga taagaaaaat agaaaaaaat aagaaagaaa taagatattt aatattggtg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttgaatataa acaaaaaaaa cacacaaagg tctagcggaa tttacagagg gtctagcaga 3180

3121 aacttatatt tgtttttttt gtgtgtttcc agatcgcctt aaatgtctcc cagatcgtct 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atttacaagt tttccagcaa aggtctagca gaatttacag atacccacaa ctcaaaggaa 3240

3181 taaatgttca aaaggtcgtt tccagatcgt cttaaatgtc tatgggtgtt gagtttcctt 3240

.......... .......... .......... .......... .......... ..........

SpeI(3245) .......... .......... .......... .......... ..........

3241 aaggactagt aattatcatt gactagccca tctcaattgg tatagtgatt aaaatcacct 3300

3241 ttcctgatca ttaatagtaa ctgatcgggt agagttaacc atatcactaa ttttagtgga 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agaccaattg agatgtatgt ctgaattagt tgttttcaaa gcaaatgaac tagcgattag 3360

3301 tctggttaac tctacataca gacttaatca acaaaagttt cgtttacttg atcgctaatc 3360

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcgctatgac ttaacggagc atgaaaccaa gctaatttta tgctgtgtgg cactactcaa 3420

3361 agcgatactg aattgcctcg tactttggtt cgattaaaat acgacacacc gtgatgagtt 3420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ccccacgatt gaaaacccta caaggaaaga acggacggta tcgttcactt ataaccaata 3480

3421 ggggtgctaa cttttgggat gttcctttct tgcctgccat agcaagtgaa tattggttat 3480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgctcagatg atgaacatca gtagggaaaa tgcttatggt gtattagcta aagcaaccag 3540

3481 gcgagtctac tacttgtagt catccctttt acgaatacca cataatcgat ttcgttggtc 3540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agagctgatg acgagaactg tggaaatcag gaatcctttg gttaaaggct ttgagatttt 3600

3541 tctcgactac tgctcttgac acctttagtc cttaggaaac caatttccga aactctaaaa 3600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccagtggaca aactatgcca agttctcaag cgaaaaatta gaattagttt ttagtgaaga 3660

3601 ggtcacctgt ttgatacggt tcaagagttc gctttttaat cttaatcaaa aatcacttct 3660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gatattgcct tatcttttcc agttaaaaaa attcataaaa tataatctgg aacatgttaa 3720

3661 ctataacgga atagaaaagg tcaatttttt taagtatttt atattagacc ttgtacaatt 3720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gtcttttgaa aacaaatact ctatgaggat ttatgagtgg ttattaaaag aactaacaca 3780

3721 cagaaaactt ttgtttatga gatactccta aatactcacc aataattttc ttgattgtgt 3780

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaagaaaact cacaaggcaa atatagagat tagccttgat gaatttaagt tcatgttaat 3840

3781 tttcttttga gtgttccgtt tatatctcta atcggaacta cttaaattca agtacaatta 3840

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcttgaaaat aactaccatg agtttaaaag gcttaaccaa tgggttttga aaccaataag 3900

3841 cgaactttta ttgatggtac tcaaattttc cgaattggtt acccaaaact ttggttattc 3900

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 taaagattta aacacttaca gcaatatgaa attggtggtt gataagcgag gccgcccgac 3960

3901 atttctaaat ttgtgaatgt cgttatactt taaccaccaa ctattcgctc cggcgggctg 3960

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgatacgttg attttccaag ttgaactaga tagacaaatg gatctcgtaa ccgaacttga 4020

3961 actatgcaac taaaaggttc aacttgatct atctgtttac ctagagcatt ggcttgaact 4020

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gaacaaccag ataaaaatga atggtgacaa aataccaaca accattacat cagattccta 4080

4021 cttgttggtc tatttttact taccactgtt ttatggttgt tggtaatgta gtctaaggat 4080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cctacgtaac ggactaagaa aaacactaca cgatgcttta actgcaaaaa ttcagctcac 4140

4081 ggatgcattg cctgattctt tttgtgatgt gctacgaaat tgacgttttt aagtcgagtg 4140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cagttttgag gcaaaatttt tgagtgacat gcaaagtaag catgatctca atggttcgtt 4200

4141 gtcaaaactc cgttttaaaa actcactgta cgtttcattc gtactagagt taccaagcaa 4200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ctcatggctc acgcaaaaac aacgaaccac actagagaac atactggcta aatacggaag 4260

4201 gagtaccgag tgcgtttttg ttgcttggtg tgatctcttg tatgaccgat ttatgccttc 4260

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gatctgaggt tcttatggct cttgtatcta tcagtgaagc atcaagacta acaaacaaaa 4320

4261 ctagactcca agaataccga gaacatagat agtcacttcg tagttctgat tgtttgtttt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .... NdeI(4365),ApaLI(4370) ....

4321 gtagaacaac tgttcaccgt tagatatcaa agggaaaact gtccatatgg tgcactctca 4380

4321 catcttgttg acaagtggca atctatagtt tcccttttga caggtatacc acgtgagagt 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga 4440

4381 catgttagac gagactacgg cgtatcaatt cggtcatatg tgaggcgata gcgatgcact 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg 4500

4441 gacccagtac cgacgcgggg ctgtgggcgg ttgtgggcga ctgcgcggga ctgcccgaac 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca 4560

4501 agacgagggc cgtaggcgaa tgtctgttcg acactggcag aggccctcga cgtacacagt 4560

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

4561 gaggttttca ccgtcatcac cgaaacgcgc gaggcagcaa ggagatggcg cccaacagtc 4620

4561 ctccaaaagt ggcagtagtg gctttgcgcg ctccgtcgtt cctctaccgc gggttgtcag 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt 4680

4621 ggggccggtg ccccggacgg tggtatgggt gcggctttgt tcgcgagtac tcgggcttca 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg 4740

4681 ccgctcgggc tagaaggggt agccactaca gccgctatat ccgcggtcgt tggcgtggac 4740

.......... .......... ........ pBRrevBam_pri ........ ..........

.......... .......... .......... .......... .......... ..........

4741 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctgctca tgtttgacag 4800

4741 accgcggcca ctacggccgg tgctacgcag gccgcatctc ctagacgagt acaaactgtc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cttatc.... .......... .......... .......... .......... .......... 4806

4801 gaatag.... .......... .......... .......... .......... .......... 4806

.......... .......... .......... .......... .......... ..........