GG+LOCUS pBAD42 6175 bp DNA SYN 29-Aug-2007 DEFINITION pBAD42 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6175 /organism="pBAD42" /mol_type="other DNA" misc_feature complement(191..1069) /label="AraC" CDS complement(191..1120) /label="ORF frame 2" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 1099..1114 /label="araO2" misc_feature 1256..1267 /label="araO1" misc_feature 1298..1311 /label="CAP_BS" misc_feature 1303..1322 /label="pBAD_fwd_primer" misc_feature 1308..1346 /label="AraI1I2" promoter 1343..1371 /label="ARA_promoter" misc_feature complement(1490..1507) /label="pBAD_rev_primer" misc_feature complement(1490..1507) /label="pTrcHis_rev_primer" terminator 1540..1697 /label="rrnB_terminator" terminator 1663..1706 /label="rrnB_T1_terminator" terminator 1838..1865 /label="rrnB_T2_terminator" promoter 1906..1934 /label="AmpR_promoter" misc_feature complement(1946..1962) /label="M13_forward20_primer" misc_feature complement(1955..1977) /label="M13_pUC_fwd_primer" misc_feature 1955..2098 /label="lacZ_a" CDS complement(2485..3495) /label="spect" /gene="spect" /note="ORF frame 1" /translation="MRSRNWSRTLTERSGGNGAVAVFMACYDCFFGVQSMPRASKQQA RYAVGRCLMLWSSNDVTQQGSRPKTKLNIMREAVIAEVSTQLSEVVGVIERHLEPTLL AVHLYGSAVDGGLKPHSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVE VTIVVHDDIIPWRYPAKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVAL VGPAAEELFDPVPEQDLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIA PKEVAADWAMERLPAQYQPVIREARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVV GK*" gene complement(2485..3495) /label="spect" /gene="spect" CDS complement(4310..5260) /label="ORF frame 2" /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTS FEAKFLSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI*" promoter 6131..6160 /label="lac_promoter" ORIGIN 1 CCTGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC 61 GGCGTAGAGG ATCTGCTCAT GTTTGACAGC TTATCATCGA TGCATAATGT GCCTGTCAAA 121 TGGACGAAGC AGGGATTCTG CAAACCCTAT GCTACTCCGT CAAGCCGTCA ATTGTCTGAT 181 TCGTTACCAA TTATGACAAC TTGACGGCTA CATCATTCAC TTTTTCTTCA CAACCGGCAC 241 GGAACTCGCT CGGGCTGGCC CCGGTGCATT TTTTAAATAC CCGCGAGAAA TAGAGTTGAT 301 CGTCAAAACC AACATTGCGA CCGACGGTGG CGATAGGCAT CCGGGTGGTG CTCAAAAGCA 361 GCTTCGCCTG GCTGATACGT TGGTCCTCGC GCCAGCTTAA GACGCTAATC CCTAACTGCT 421 GGCGGAAAAG ATGTGACAGA CGCGACGGCG ACAAGCAAAC ATGCTGTGCG ACGCTGGCGA 481 TATCAAAATT GCTGTCTGCC AGGTGATCGC TGATGTACTG ACAAGCCTCG CGTACCCGAT 541 TATCCATCGG TGGATGGAGC GACTCGTTAA TCGCTTCCAT GCGCCGCAGT AACAATTGCT 601 CAAGCAGATT TATCGCCAGC AGCTCCGAAT AGCGCCCTTC CCCTTGCCCG GCGTTAATGA 661 TTTGCCCAAA CAGGTCGCTG AAATGCGGCT GGTGCGCTTC ATCCGGGCGA AAGAACCCCG 721 TATTGGCAAA TATTGACGGC CAGTTAAGCC ATTCATGCCA GTAGGCGCGC GGACGAAAGT 781 AAACCCACTG GTGATACCAT TCGCGAGCCT CCGGATGACG ACCGTAGTGA TGAATCTCTC 841 CTGGCGGGAA CAGCAAAATA TCACCCGGTC GGCAAACAAA TTCTCGTCCC TGATTTTTCA 901 CCACCCCCTG ACCGCGAATG GTGAGATTGA GAATATAACC TTTCATTCCC AGCGGTCGGT 961 CGATAAAAAA ATCGAGATAA CCGTTGGCCT CAATCGGCGT TAAACCCGCC ACCAGATGGG 1021 CATTAAACGA GTATCCCGGC AGCAGGGGAT CATTTTGCGC TTCAGCCATA CTTTTCATAC 1081 TCCCGCCATT CAGAGAAGAA ACCAATTGTC CATATTGCAT CAGACATTGC CGTCACTGCG 1141 TCTTTTACTG GCTCTTCTCG CTAACCAAAC CGGTAACCCC GCTTATTAAA AGCATTCTGT 1201 AACAAAGCGG GACCAAAGCC ATGACAAAAA CGCGTAACAA AAGTGTCTAT AATCACGGCA 1261 GAAAAGTCCA CATTGATTAT TTGCACGGCG TCACACTTTG CTATGCCATA GCATTTTTAT 1321 CCATAAGATT AGCGGATCCT ACCTGACGCT TTTTATCGCA ACTCTCTACT GTTTCTCCAT 1381 ACCCGTTTTT TTGGGCTAGC GAATTCGAGC TCGGTACCCG GGGATCCTCT AGAGTCGACC 1441 TGCAGGCATG CAAGCTTGGC TGTTTTGGCG GATGAGAGAA GATTTTCAGC CTGATACAGA 1501 TTAAATCAGA ACGCAGAAGC GGTCTGATAA AACAGAATTT GCCTGGCGGC AGTAGCGCGG 1561 TGGTCCCACC TGACCCCATG CCGAACTCAG AAGTGAAACG CCGTAGCGCC GATGGTAGTG 1621 TGGGGTCTCC CCATGCGAGA GTAGGGAACT GCCAGGCATC AAATAAAACG AAAGGCTCAG 1681 TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT CCTGAGTAGG 1741 ACAAATCCGC CGGGAGCGGA TTTGAACGTT GCGAAGCAAC GGCCCGGAGG GTGGCGGGCA 1801 GGACGCCCGC CATAAACTGC CAGGCATCAA ATTAAGCAGA AGGCCATCCT GACGGATGGC 1861 CTTTTTGCGT TTCTACAAAC TCTTTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 1921 GCTCATGAGA CAATAACCCT AGCTTACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA 1981 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA 2041 ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT 2101 GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATGGT 2161 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGCCCCGA CACCCGCCAA 2221 CACCCGCTGA CGAGCTTAGT AAAGCCCTCG CTAGATTTTA ATGCGGATGT TGCGATTACT 2281 TCGCCAACTA TTGCGATAAC AAGAAAAAGC CAGCCTTTCA TGATATATCT CCCAATTTGT 2341 GTAGGGCTTA TTATGCACGC TTAAAAATAA TAAAAGCAGA CTTGACCTGA TAGTTTGGCT 2401 GTGAGCAATT ATGTGCTTAG TGCATCTAAC GCTTGAGTTA AGCCGCGCCG CGAAGCGGCG 2461 TCGGCTTGAA CGAATTGTTA GACATTATTT GCCGACTACC TTGGTGATCT CGCCTTTCAC 2521 GTAGTGGACA AATTCTTCCA ACTGATCTGC GCGCGAGGCC AAGCGATCTT CTTCTTGTCC 2581 AAGATAAGCC TGTCTAGCTT CACGTATGAC GGGCTGATAC TGGGCCGGCA GGCGCTCCAT 2641 TGCCCAGTCG GCAGCGACCT CCTTCGGCGC GATTTTGCCG GTTACTGCGC TGTACCAAAT 2701 GCGGGACAAC GTAAGCACTA CATTTCGCTC ATCGCCAGCC CAGTCGGGCG GCGAGTTCCA 2761 TAGCGTTAAG GTTTCATTTA GCGCCTCAAA TAGATCCTGT TCAGGAACCG GATCAAAGAG 2821 TTCCTCCGCC GCTGGACCTA CCAAGGCAAC GCTATGTTCT CTTGCTTTTG TCAGCAAGAT 2881 AGCCAGATCA ATGTCGATCG TGGCTGGCTC GAAGATACCT GCAAGAATGT CATTGCGCTG 2941 CCATTCTCCA AATTGCAGTT CGCGCTTAGC TGGATAACGC CACGGAATGA TGTCGTCGTG 3001 CACAACAATG GTGACTTCTA CAGCGCGGAG AATCTCGCTC TCTCCAGGGG AAGCCGAAGT 3061 TTCCAAAAGG TCGTTGATCA AAGCTCGCCG CGTTGTTTCA TCAAGCCTTA CGGTCACCGT 3121 AACCAGCAAA TCAATATCAC TGTGTGGCTT CAGGCCGCCA TCCACTGCGG AGCCGTACAA 3181 ATGTACGGCC AGCAACGTCG GTTCGAGATG GCGCTCGATG ACGCCAACTA CCTCTGATAG 3241 TTGAGTCGAT ACTTCGGCGA TCACCGCTTC CCTCATGATG TTTAACTTTG TTTTAGGGCG 3301 ACTGCCCTGC TGCGTAACAT CGTTGCTGCT CCATAACATC AAACATCGAC CCACGGCGTA 3361 ACGCGCTTGC TGCTTGGATG CCCGAGGCAT AGACTGTACC CCAAAAAAAC AGTCATAACA 3421 AGCCATGAAA ACCGCCACTG CGCCGTTACC ACCGCTGCGT TCGGTCAAGG TTCTGGACCA 3481 GTTGCGTGAG CGCATACGCT ACTTGCATTA CAGCTTACGA ACCGAACAGG CTTATGTCCA 3541 CTGGGTTCGT GCCTTCATCC GTTTCCACGG TGTGCGTCAC CCGGCAACCT TGGGCAGCAG 3601 CGAAGTCGAG GCATTTCTGT CCTGGCTGGC GAACGAGCGC AAGGTTTCGG TCTCCACGCA 3661 TCGTCAGGCA TTGGCGGCCT TGCTGTTCTT CTACGGCAAG GTGCTGTGCA CGGATCTGCC 3721 CTGGCTTCAG GAGATCGGAA GACCTCGGCC GTCGCGGCGC TTGCCGGTGG TGCTGACCCC 3781 GGATGAAGTA ATTCCCACGG GTTTTGCTGC CCGCAAACGG GCTGTTCTGG TGTTGCTAGT 3841 TTGTTATCAG AATCGCAGAT CCGGCTTCAG GTTTGCCGGC TGAAAGCGCT ATTTCTTCCA 3901 GAATTGCCAT GATTTTTTCC CCACGGGAGG CGTCACTGGC TCCCGTGTTG TCGGCAGCTT 3961 TGATTCGATA AGCAGCATCG CCTGTTTCAG GCTGTCTATG TGTGACTGTT GAGCTGTAAC 4021 AAGTTGTCTC AGGTGTTCAA TTTCATGTTC TAGTTGCTTT GTTTTACTGG TTTCACCTGT 4081 TCTATTAGGT GTTACATGCT GTTCATCTGT TACATTGTCG ATCTGTTCAT GGTGAACAGC 4141 TTTGAATGCA CCAAAAACTC GTAAAAGCTC TGATGTATCT ATCTTTTTTA CACCGTTTTC 4201 ATCTGTGCAT ATGGACAGTT TTCCCTTTGA TATGTAACGG TGAACAGTTG TTCTACTTTT 4261 GTTTGTTAGT CTTGATGCTT CACTGATAGA TACAAGAGCC ATAAGAACCT CAGATCCTTC 4321 CGTATTTAGC CAGTATGTTC TCTAGTGTGG TTCGTTGTTT TTGCGTGAGC CATGAGAACG 4381 AACCATTGAG ATCATACTTA CTTTGCATGT CACTCAAAAA TTTTGCCTCA AAACTGGTGA 4441 GCTGAATTTT TGCAGTTAAA GCATCGTGTA GTGTTTTTCT TAGTCCGTTA TGTAGGTAGG 4501 AATCTGATGT AATGGTTGTT GGTATTTTGT CACCATTCAT TTTTATCTGG TTGTTCTCAA 4561 GTTCGGTTAC GAGATCCATT TGTCTATCTA GTTCAACTTG GAAAATCAAC GTATCAGTCG 4621 GGCGGCCTCG CTTATCAACC ACCAATTTCA TATTGCTGTA AGTGTTTAAA TCTTTACTTA 4681 TTGGTTTCAA AACCCATTGG TTAAGCCTTT TAAACTCATG GTAGTTATTT TCAAGCATTA 4741 ACATGAACTT AAATTCATCA AGGCTAATCT CTATATTTGC CTTGTGAGTT TTCTTTTGTG 4801 TTAGTTCTTT TAATAACCAC TCATAAATCC TCATAGAGTA TTTGTTTTCA AAAGACTTAA 4861 CATGTTCCAG ATTATATTTT ATGAATTTTT TTAACTGGAA AAGATAAGGC AATATCTCTT 4921 CACTAAAAAC TAATTCTAAT TTTTCGCTTG AGAACTTGGC ATAGTTTGTC CACTGGAAAA 4981 TCTCAAAGCC TTTAACCAAA GGATTCCTGA TTTCCACAGT TCTCGTCATC AGCTCTCTGG 5041 TTGCTTTAGC TAATACACCA TAAGCATTTT CCCTACTGAT GTTCATCATC TGAGCGTATT 5101 GGTTATAAGT GAACGATACC GTCCGTTCTT TCCTTGTAGG GTTTTCAATC GTGGGGTTGA 5161 GTAGTGCCAC ACAGCATAAA ATTAGCTTGG TTTCATGCTC CGTTAAGTCA TAGCGACTAA 5221 TCGCTAGTTC ATTTGCTTTG AAAACAACTA ATTCAGACAT ACATCTCAAT TGGTCTAGGT 5281 GATTTTAATC ACTATACCAA TTGAGATGGG CTAGTCAATG ATAATTACTA GTCCTTTTCC 5341 TTTGAGTTGT GGGTATCTGT AAATTCTGCT AGACCTTTGC TGGAAAACTT GTAAATTCTG 5401 CTAGACCCTC TGTAAATTCC GCTAGACCTT TGTGTGTTTT TTTTGTTTAT ATTCAAGTGG 5461 TTATAATTTA TAGAATAAAG AAAGAATAAA AAAAGATAAA AAGAATAGAT CCCAGCCCTG 5521 TGTATAACTC ACTACTTTAG TCAGTTCCGC AGTATTACAA AAGGATGTCG CAAACGCTGT 5581 TTGCTCCTCT ACAAAACAGA CCTTAAAACC CTAAAGGCTT AAGTAGCACC CTCGCAAGCT 5641 CGGGCAAATC GCTGAATATT CCTTTTGTCT CCGACCATCA GGCACCTGAG TCGCTGTCTT 5701 TTTCGTGACA TTCAGTTCGC TGCGCTCACG GCTCTGGCAG TGAATGGGGG TAAATGGCAC 5761 TACAGGCGCC TTTTATGGAT TCATGCAAGG AAACTACCCA TAATACAAGA AAAGCCCGTC 5821 ACGGGCTTCT CAGGGCGTTT TATGGCGGGT CTGCTATGTG GTGCTATCTG ACTTTTTGCT 5881 GTTCAGCAGT TCCTGCCCTC TGATTTTCCA GTCTGACCAC TTCGGATTAT CCCGTGACAG 5941 GTCATTCAGA CTGGCTAATG CACCCAGTAA GGCAGCGGTA TCATCAACAG GCTTACCCGT 6001 CTTACTGTCG GGAATTAATT CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC 6061 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG 6121 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAG // pBAD42 "Times New Roman GC-AraC"Times New RomanGC`ORF 2"Times New RomanGCɆKZaraO2"Times New RomanGaraO1"Times New RomanGCCAP_BS"Times New RomanGC* pBAD_fwd_pri"Times New RomanGCɆBAraI1I2"Times New Roman*G?[ARA_pro"Times New RomanGC pBAD_rev_pri"Times New RomanGCpTrcHis_rev_pri"Times New RomanGCɆ rrnB_term"Times New RomanG rrnB_T1_term"Times New RomanGC.I rrnB_T2_term"Times New RomanGCrAmpR_pro"Times New RomanGCɆM13_forward20_pri"Times New RomanGM13_pUC_fwd_pri"Times New RomanGC2lacZ_a"Times New Roman*GC ORF 1"Times New RomanGCɆ spect"Times New RomanGORF 2"Times New RomanGClac_pro"Times New Roman GClaI"Times New RomanaG3 GEcoRV"Times New Romana%P.> /P> GNruI"Times New Roman#Ucqqqbq GAgeI"Times New Roman GfNheI(1395),EcoRI(1401),SacI(1411),KpnI(1417),SmaI(1419),XbaI(1428),SalI(1434),PstI(1444),HindIII(1452)"Times New Romans W GFspI"Times New Roman"u GBclI"Times New Roman &99H GEagI"Times New Roman"   GSpeI"Times New Romanp Gtest"Times New RomanvvZ4 Gtest"Times New Romano{s{b]bl