Sequence of pBAD42

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Sequence of pBAD42

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Sequence of pBAD42 : 6175 BP; 1491 A; 1538 C; 1413 G; 1733 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cctgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc 60

1 ggactatatc cgcggtcgtt ggcgtggaca ccgcggccac tacggccggt gctacgcagg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ClaI(97) .......... ..........

61 ggcgtagagg atctgctcat gtttgacagc ttatcatcga tgcataatgt gcctgtcaaa 120

61 ccgcatctcc tagacgagta caaactgtcg aatagtagct acgtattaca cggacagttt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat 180

121 acctgcttcg tccctaagac gtttgggata cgatgaggca gttcggcagt taacagacta 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac 240

181 agcaatggtt aatactgttg aactgccgat gtagtaagtg aaaaagaagt gttggccgtg 240

.......... .......... .......... AraC ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat 300

241 ccttgagcga gcccgaccgg ggccacgtaa aaaatttatg ggcgctcttt atctcaacta 300

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca 360

301 gcagttttgg ttgtaacgct ggctgccacc gctatccgta ggcccaccac gagttttcgt 360

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct 420

361 cgaagcggac cgactatgca accaggagcg cggtcgaatt ctgcgattag ggattgacga 420

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga 480

421 ccgccttttc tacactgtct gcgctgccgc tgttcgtttg tacgacacgc tgcgaccgct 480

.......... .......... AraC ORF 2 .......... .......... ..........

EcoRV(481) .......... .......... .......... .......... ..........

481 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat 540

481 atagttttaa cgacagacgg tccactagcg actacatgac tgttcggagc gcatgggcta 540

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct 600

541 ataggtagcc acctacctcg ctgagcaatt agcgaaggta cgcggcgtca ttgttaacga 600

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga 660

601 gttcgtctaa atagcggtcg tcgaggctta tcgcgggaag gggaacgggc cgcaattact 660

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg 720

661 aaacgggttt gtccagcgac tttacgccga ccacgcgaag taggcccgct ttcttggggc 720

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt 780

721 ataaccgttt ataactgccg gtcaattcgg taagtacggt catccgcgcg cctgctttca 780

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... NruI(803) .......... .......... ..........

781 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc 840

781 tttgggtgac cactatggta agcgctcgga ggcctactgc tggcatcact acttagagag 840

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca 900

841 gaccgccctt gtcgttttat agtgggccag ccgtttgttt aagagcaggg actaaaaagt 900

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt 960

901 ggtgggggac tggcgcttac cactctaact cttatattgg aaagtaaggg tcgccagcca 960

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg 1020

961 gctatttttt tagctctatt ggcaaccgga gttagccgca atttgggcgg tggtctaccc 1020

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac 1080

1021 gtaatttgct catagggccg tcgtccccta gtaaaacgcg aagtcggtat gaaaagtatg 1080

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg 1140

1081 agggcggtaa gtctcttctt tggttaacag gtataacgta gtctgtaacg gcagtgacgc 1140

.......... ORF 2 araO2 .......... .......... ..........

.......... .......... AgeI(1169) .......... .......... ..........

1141 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt 1200

1141 agaaaatgac cgagaagagc gattggtttg gccattgggg cgaataattt tcgtaagaca 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca 1260

1201 ttgtttcgcc ctggtttcgg tactgttttt gcgcattgtt ttcacagata ttagtgccgt 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1320

1261 cttttcaggt gtaactaata aacgtgccgc agtgtgaaac gatacggtat cgtaaaaata 1320

.......... .......... .......... ..... CAP_BS pBAD_fwd_pri AraI1I2

.......... .......... .......... .......... .......... ..........

1321 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1380

1321 ggtattctaa tcgcctagga tggactgcga aaaatagcgt tgagagatga caaagaggta 1380

.......... AraI1I2 .......... ARA_pro .......... ..........

NheI EcoRI SacI) KpnI(1417),SmaI(1419) XbaI(1428)

1381 acccgttttt ttgggctagc gaattcgagc tcggtacccg gggatcctct agagtcgacc 1440

1381 tgggcaaaaa aacccgatcg cttaagctcg agccatgggc ccctaggaga tctcagctgg 1440

.......... .......... .......... .......... .......... ..........

PstI(1444) HindIII(1452) ...... .......... .......... ..........

1441 tgcaggcatg caagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga 1500

1441 acgtccgtac gttcgaaccg acaaaaccgc ctactctctt ctaaaagtcg gactatgtct 1500

.......... .......... .......... .......... . pBAD_rev_pri pTrcHis_rev_pri

.......... .......... .......... .......... .......... ..........

1501 ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg 1560

1501 aatttagtct tgcgtcttcg ccagactatt ttgtcttaaa cggaccgccg tcatcgcgcc 1560

.......... .......... .......... .......... rrnB_term ..........

.......... .......... .......... .......... .......... ..........

1561 tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg 1620

1561 accagggtgg actggggtac ggcttgagtc ttcactttgc ggcatcgcgg ctaccatcac 1620

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag 1680

1621 accccagagg ggtacgctct catcccttga cggtccgtag tttattttgc tttccgagtc 1680

.......... .......... rrnB_term .......... ......... rrnB_T1_term

.......... .......... .......... .......... .......... ..........

1681 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg 1740

1681 agctttctga cccggaaagc aaaatagaca acaaacagcc acttgcgaga ggactcatcc 1740

rrnB_term rrnB_T1_term ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca 1800

1741 tgtttaggcg gccctcgcct aaacttgcaa cgcttcgttg ccgggcctcc caccgcccgt 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc 1860

1801 cctgcgggcg gtatttgacg gtccgtagtt taattcgtct tccggtagga ctgcctaccg 1860

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

1861 ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa atatgtatcc 1920

1861 gaaaaacgca aagatgtttg agaaaacaaa taaaaagatt tatgtaagtt tatacatagg 1920

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

1921 gctcatgaga caataaccct agcttactgg ccgtcgtttt acaacgtcgt gactgggaaa 1980

1921 cgagtactct gttattggga tcgaatgacc ggcagcaaaa tgttgcagca ctgacccttt 1980

AmpR_pro .......... M13_forward20_pri M13_pUC_fwd_pri lacZ_a

.......... .......... .......... .......... .......... ..........

1981 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 2040

1981 tgggaccgca atgggttgaa ttagcggaac gtcgtgtagg gggaaagcgg tcgaccgcat 2040

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... FspI(2082) ..........

2041 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 2100

2041 tatcgcttct ccgggcgtgg ctagcgggaa gggttgtcaa cgcgtcggac ttaccgctta 2100

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt 2160

2101 ccgcggacta cgccataaaa gaggaatgcg tagacacgcc ataaagtgtg gcgtatacca 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 2220

2161 cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcggggct gtgggcggtt 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cacccgctga cgagcttagt aaagccctcg ctagatttta atgcggatgt tgcgattact 2280

2221 gtgggcgact gctcgaatca tttcgggagc gatctaaaat tacgcctaca acgctaatga 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcgccaacta ttgcgataac aagaaaaagc cagcctttca tgatatatct cccaatttgt 2340

2281 agcggttgat aacgctattg ttctttttcg gtcggaaagt actatataga gggttaaaca 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gtagggctta ttatgcacgc ttaaaaataa taaaagcaga cttgacctga tagtttggct 2400

2341 catcccgaat aatacgtgcg aatttttatt attttcgtct gaactggact atcaaaccga 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtgagcaatt atgtgcttag tgcatctaac gcttgagtta agccgcgccg cgaagcggcg 2460

2401 cactcgttaa tacacgaatc acgtagattg cgaactcaat tcggcgcggc gcttcgccgc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac 2520

2461 agccgaactt gcttaacaat ctgtaataaa cggctgatgg aaccactaga gcggaaagtg 2520

.......... .......... .......... ......... ORF 1 spect .........

.......... .......... .......... .......... .......... ..........

2521 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc 2580

2521 catcacctgt ttaagaaggt tgactagacg cgcgctccgg ttcgctagaa gaagaacagg 2580

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aagataagcc tgtctagctt cacgtatgac gggctgatac tgggccggca ggcgctccat 2640

2581 ttctattcgg acagatcgaa gtgcatactg cccgactatg acccggccgt ccgcgaggta 2640

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tgcccagtcg gcagcgacct ccttcggcgc gattttgccg gttactgcgc tgtaccaaat 2700

2641 acgggtcagc cgtcgctgga ggaagccgcg ctaaaacggc caatgacgcg acatggttta 2700

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca 2760

2701 cgccctgttg cattcgtgat gtaaagcgag tagcggtcgg gtcagcccgc cgctcaaggt 2760

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag 2820

2761 atcgcaattc caaagtaaat cgcggagttt atctaggaca agtccttggc ctagtttctc 2820

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat 2880

2821 aaggaggcgg cgacctggat ggttccgttg cgatacaaga gaacgaaaac agtcgttcta 2880

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg 2940

2881 tcggtctagt tacagctagc accgaccgag cttctatgga cgttcttaca gtaacgcgac 2940

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg 3000

2941 ggtaagaggt ttaacgtcaa gcgcgaatcg acctattgcg gtgccttact acagcagcac 3000

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt 3060

3001 gtgttgttac cactgaagat gtcgcgcctc ttagagcgag agaggtcccc ttcggcttca 3060

.......... ......... ORF 1 spect ......... .......... ..........

.......... BclI(3075) .......... .......... .......... ..........

3061 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt 3120

3061 aaggttttcc agcaactagt ttcgagcggc gcaacaaagt agttcggaat gccagtggca 3120

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa 3180

3121 ttggtcgttt agttatagtg acacaccgaa gtccggcggt aggtgacgcc tcggcatgtt 3180

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag 3240

3181 tacatgccgg tcgttgcagc caagctctac cgcgagctac tgcggttgat ggagactatc 3240

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg ttttagggcg 3300

3241 aactcagcta tgaagccgct agtggcgaag ggagtactac aaattgaaac aaaatcccgc 3300

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta 3360

3301 tgacgggacg acgcattgta gcaacgacga ggtattgtag tttgtagctg ggtgccgcat 3360

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca 3420

3361 tgcgcgaacg acgaacctac gggctccgta tctgacatgg ggtttttttg tcagtattgt 3420

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca 3480

3421 tcggtacttt tggcggtgac gcggcaatgg tggcgacgca agccagttcc aagacctggt 3480

.......... ......... ORF 1 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg cttatgtcca 3540

3481 caacgcactc gcgtatgcga tgaacgtaat gtcgaatgct tggcttgtcc gaatacaggt 3540

ORF 1 spect ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag 3600

3541 gacccaagca cggaagtagg caaaggtgcc acacgcagtg ggccgttgga acccgtcgtc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca 3660

3601 gcttcagctc cgtaaagaca ggaccgaccg cttgctcgcg ttccaaagcc agaggtgcgt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc 3720

3661 agcagtccgt aaccgccgga acgacaagaa gatgccgttc cacgacacgt gcctagacgg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... EagI(3746) .......... .......... ..........

3721 ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc 3780

3721 gaccgaagtc ctctagcctt ctggagccgg cagcgccgcg aacggccacc acgactgggg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggatgaagta attcccacgg gttttgctgc ccgcaaacgg gctgttctgg tgttgctagt 3840

3781 cctacttcat taagggtgcc caaaacgacg ggcgtttgcc cgacaagacc acaacgatca 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttgttatcag aatcgcagat ccggcttcag gtttgccggc tgaaagcgct atttcttcca 3900

3841 aacaatagtc ttagcgtcta ggccgaagtc caaacggccg actttcgcga taaagaaggt 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gaattgccat gattttttcc ccacgggagg cgtcactggc tcccgtgttg tcggcagctt 3960

3901 cttaacggta ctaaaaaagg ggtgccctcc gcagtgaccg agggcacaac agccgtcgaa 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgattcgata agcagcatcg cctgtttcag gctgtctatg tgtgactgtt gagctgtaac 4020

3961 actaagctat tcgtcgtagc ggacaaagtc cgacagatac acactgacaa ctcgacattg 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aagttgtctc aggtgttcaa tttcatgttc tagttgcttt gttttactgg tttcacctgt 4080

4021 ttcaacagag tccacaagtt aaagtacaag atcaacgaaa caaaatgacc aaagtggaca 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tctattaggt gttacatgct gttcatctgt tacattgtcg atctgttcat ggtgaacagc 4140

4081 agataatcca caatgtacga caagtagaca atgtaacagc tagacaagta ccacttgtcg 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tttgaatgca ccaaaaactc gtaaaagctc tgatgtatct atctttttta caccgttttc 4200

4141 aaacttacgt ggtttttgag cattttcgag actacataga tagaaaaaat gtggcaaaag 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 atctgtgcat atggacagtt ttccctttga tatgtaacgg tgaacagttg ttctactttt 4260

4201 tagacacgta tacctgtcaa aagggaaact atacattgcc acttgtcaac aagatgaaaa 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtttgttagt cttgatgctt cactgataga tacaagagcc ataagaacct cagatccttc 4320

4261 caaacaatca gaactacgaa gtgactatct atgttctcgg tattcttgga gtctaggaag 4320

.......... .......... .......... .......... .......... ORF 2

.......... .......... .......... .......... .......... ..........

4321 cgtatttagc cagtatgttc tctagtgtgg ttcgttgttt ttgcgtgagc catgagaacg 4380

4321 gcataaatcg gtcatacaag agatcacacc aagcaacaaa aacgcactcg gtactcttgc 4380

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aaccattgag atcatactta ctttgcatgt cactcaaaaa ttttgcctca aaactggtga 4440

4381 ttggtaactc tagtatgaat gaaacgtaca gtgagttttt aaaacggagt tttgaccact 4440

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gctgaatttt tgcagttaaa gcatcgtgta gtgtttttct tagtccgtta tgtaggtagg 4500

4441 cgacttaaaa acgtcaattt cgtagcacat cacaaaaaga atcaggcaat acatccatcc 4500

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aatctgatgt aatggttgtt ggtattttgt caccattcat ttttatctgg ttgttctcaa 4560

4501 ttagactaca ttaccaacaa ccataaaaca gtggtaagta aaaatagacc aacaagagtt 4560

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gttcggttac gagatccatt tgtctatcta gttcaacttg gaaaatcaac gtatcagtcg 4620

4561 caagccaatg ctctaggtaa acagatagat caagttgaac cttttagttg catagtcagc 4620

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ggcggcctcg cttatcaacc accaatttca tattgctgta agtgtttaaa tctttactta 4680

4621 ccgccggagc gaatagttgg tggttaaagt ataacgacat tcacaaattt agaaatgaat 4680

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ttggtttcaa aacccattgg ttaagccttt taaactcatg gtagttattt tcaagcatta 4740

4681 aaccaaagtt ttgggtaacc aattcggaaa atttgagtac catcaataaa agttcgtaat 4740

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 acatgaactt aaattcatca aggctaatct ctatatttgc cttgtgagtt ttcttttgtg 4800

4741 tgtacttgaa tttaagtagt tccgattaga gatataaacg gaacactcaa aagaaaacac 4800

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttagttcttt taataaccac tcataaatcc tcatagagta tttgttttca aaagacttaa 4860

4801 aatcaagaaa attattggtg agtatttagg agtatctcat aaacaaaagt tttctgaatt 4860

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 catgttccag attatatttt atgaattttt ttaactggaa aagataaggc aatatctctt 4920

4861 gtacaaggtc taatataaaa tacttaaaaa aattgacctt ttctattccg ttatagagaa 4920

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cactaaaaac taattctaat ttttcgcttg agaacttggc atagtttgtc cactggaaaa 4980

4921 gtgatttttg attaagatta aaaagcgaac tcttgaaccg tatcaaacag gtgacctttt 4980

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tctcaaagcc tttaaccaaa ggattcctga tttccacagt tctcgtcatc agctctctgg 5040

4981 agagtttcgg aaattggttt cctaaggact aaaggtgtca agagcagtag tcgagagacc 5040

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttgctttagc taatacacca taagcatttt ccctactgat gttcatcatc tgagcgtatt 5100

5041 aacgaaatcg attatgtggt attcgtaaaa gggatgacta caagtagtag actcgcataa 5100

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ggttataagt gaacgatacc gtccgttctt tccttgtagg gttttcaatc gtggggttga 5160

5101 ccaatattca cttgctatgg caggcaagaa aggaacatcc caaaagttag caccccaact 5160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gtagtgccac acagcataaa attagcttgg tttcatgctc cgttaagtca tagcgactaa 5220

5161 catcacggtg tgtcgtattt taatcgaacc aaagtacgag gcaattcagt atcgctgatt 5220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tcgctagttc atttgctttg aaaacaacta attcagacat acatctcaat tggtctaggt 5280

5221 agcgatcaag taaacgaaac ttttgttgat taagtctgta tgtagagtta accagatcca 5280

.......... ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... SpeI(5327) ..........

5281 gattttaatc actataccaa ttgagatggg ctagtcaatg ataattacta gtccttttcc 5340

5281 ctaaaattag tgatatggtt aactctaccc gatcagttac tattaatgat caggaaaagg 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tttgagttgt gggtatctgt aaattctgct agacctttgc tggaaaactt gtaaattctg 5400

5341 aaactcaaca cccatagaca tttaagacga tctggaaacg accttttgaa catttaagac 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ctagaccctc tgtaaattcc gctagacctt tgtgtgtttt ttttgtttat attcaagtgg 5460

5401 gatctgggag acatttaagg cgatctggaa acacacaaaa aaaacaaata taagttcacc 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ttataattta tagaataaag aaagaataaa aaaagataaa aagaatagat cccagccctg 5520

5461 aatattaaat atcttatttc tttcttattt ttttctattt ttcttatcta gggtcgggac 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tgtataactc actactttag tcagttccgc agtattacaa aaggatgtcg caaacgctgt 5580

5521 acatattgag tgatgaaatc agtcaaggcg tcataatgtt ttcctacagc gtttgcgaca 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ttgctcctct acaaaacaga ccttaaaacc ctaaaggctt aagtagcacc ctcgcaagct 5640

5581 aacgaggaga tgttttgtct ggaattttgg gatttccgaa ttcatcgtgg gagcgttcga 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt 5700

5641 gcccgtttag cgacttataa ggaaaacaga ggctggtagt ccgtggactc agcgacagaa 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac 5760

5701 aaagcactgt aagtcaagcg acgcgagtgc cgagaccgtc acttaccccc atttaccgtg 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga aaagcccgtc 5820

5761 atgtccgcgg aaaataccta agtacgttcc tttgatgggt attatgttct tttcgggcag 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg actttttgct 5880

5821 tgcccgaaga gtcccgcaaa ataccgccca gacgatacac cacgatagac tgaaaaacga 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat cccgtgacag 5940

5881 caagtcgtca aggacgggag actaaaaggt cagactggtg aagcctaata gggcactgtc 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gtcattcaga ctggctaatg cacccagtaa ggcagcggta tcatcaacag gcttacccgt 6000

5941 cagtaagtct gaccgattac gtgggtcatt ccgtcgccat agtagttgtc cgaatgggca 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 cttactgtcg ggaattaatt cgttggccga ttcattaatg cagctggcac gacaggtttc 6060

6001 gaatgacagc ccttaattaa gcaaccggct aagtaattac gtcgaccgtg ctgtccaaag 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 6120

6061 ggctgacctt tcgcccgtca ctcgcgttgc gttaattaca ctcaatcgag tgagtaatcc 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgag..... 6175

6121 gtggggtccg aaatgtgaaa tacgaaggcc gagcatacaa cacaccttaa cactc..... 6175

.......... .......... lac_pro .......... .......... ..........