Sequence of pBAD43

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pBAD43

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pBAD43 : 6179 BP; 1494 A; 1538 C; 1414 G; 1733 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cctgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc 60

1 ggactatatc cgcggtcgtt ggcgtggaca ccgcggccac tacggccggt gctacgcagg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ClaI(97) .......... ..........

61 ggcgtagagg atctgctcat gtttgacagc ttatcatcga tgcataatgt gcctgtcaaa 120

61 ccgcatctcc tagacgagta caaactgtcg aatagtagct acgtattaca cggacagttt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat 180

121 acctgcttcg tccctaagac gtttgggata cgatgaggca gttcggcagt taacagacta 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac 240

181 agcaatggtt aatactgttg aactgccgat gtagtaagtg aaaaagaagt gttggccgtg 240

.......... .......... .......... AraC ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat 300

241 ccttgagcga gcccgaccgg ggccacgtaa aaaatttatg ggcgctcttt atctcaacta 300

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca 360

301 gcagttttgg ttgtaacgct ggctgccacc gctatccgta ggcccaccac gagttttcgt 360

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct 420

361 cgaagcggac cgactatgca accaggagcg cggtcgaatt ctgcgattag ggattgacga 420

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga 480

421 ccgccttttc tacactgtct gcgctgccgc tgttcgtttg tacgacacgc tgcgaccgct 480

.......... .......... AraC ORF 2 .......... .......... ..........

EcoRV(481) .......... .......... .......... .......... ..........

481 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat 540

481 atagttttaa cgacagacgg tccactagcg actacatgac tgttcggagc gcatgggcta 540

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct 600

541 ataggtagcc acctacctcg ctgagcaatt agcgaaggta cgcggcgtca ttgttaacga 600

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga 660

601 gttcgtctaa atagcggtcg tcgaggctta tcgcgggaag gggaacgggc cgcaattact 660

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg 720

661 aaacgggttt gtccagcgac tttacgccga ccacgcgaag taggcccgct ttcttggggc 720

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt 780

721 ataaccgttt ataactgccg gtcaattcgg taagtacggt catccgcgcg cctgctttca 780

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... NruI(803) .......... .......... ..........

781 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc 840

781 tttgggtgac cactatggta agcgctcgga ggcctactgc tggcatcact acttagagag 840

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca 900

841 gaccgccctt gtcgttttat agtgggccag ccgtttgttt aagagcaggg actaaaaagt 900

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt 960

901 ggtgggggac tggcgcttac cactctaact cttatattgg aaagtaaggg tcgccagcca 960

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg 1020

961 gctatttttt tagctctatt ggcaaccgga gttagccgca atttgggcgg tggtctaccc 1020

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac 1080

1021 gtaatttgct catagggccg tcgtccccta gtaaaacgcg aagtcggtat gaaaagtatg 1080

.......... .......... AraC ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg 1140

1081 agggcggtaa gtctcttctt tggttaacag gtataacgta gtctgtaacg gcagtgacgc 1140

.......... ORF 2 araO2 .......... .......... ..........

.......... .......... AgeI(1169) .......... .......... ..........

1141 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt 1200

1141 agaaaatgac cgagaagagc gattggtttg gccattgggg cgaataattt tcgtaagaca 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca 1260

1201 ttgtttcgcc ctggtttcgg tactgttttt gcgcattgtt ttcacagata ttagtgccgt 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1320

1261 cttttcaggt gtaactaata aacgtgccgc agtgtgaaac gatacggtat cgtaaaaata 1320

.......... .......... .......... ..... CAP_BS pBAD_fwd_pri AraI1I2

.......... .......... .......... .......... .......... ..........

1321 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1380

1321 ggtattctaa tcgcctagga tggactgcga aaaatagcgt tgagagatga caaagaggta 1380

.......... AraI1I2 .......... ARA_pro .......... ..........

... NheI EcoRI NcoI) KpnI(1421),SmaI(1423) XbaI(1432)

1381 acccgttttt ttgggctagc aggaggaatt caccatggta cccggggatc ctctagagtc 1440

1381 tgggcaaaaa aacccgatcg tcctccttaa gtggtaccat gggcccctag gagatctcag 1440

.......... .......... .......... .......... .......... ..........

PstI(1448) HindIII(1456) ...... .......... .......... ..........

1441 gacctgcagg catgcaagct tggctgtttt ggcggatgag agaagatttt cagcctgata 1500

1441 ctggacgtcc gtacgttcga accgacaaaa ccgcctactc tcttctaaaa gtcggactat 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc 1560

1501 gtctaattta gtcttgcgtc ttcgccagac tattttgtct taaacggacc gccgtcatcg 1560

pBAD_rev_pri pTrcHis_rev_pri .. .......... .......... rrnB_term

.......... .......... .......... .......... .......... ..........

1561 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt 1620

1561 cgccaccagg gtggactggg gtacggcttg agtcttcact ttgcggcatc gcggctacca 1620

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc 1680

1621 tcacacccca gaggggtacg ctctcatccc ttgacggtcc gtagtttatt ttgctttccg 1680

.......... .......... rrnB_term .......... ......... rrnB_T1_term

.......... .......... .......... .......... .......... ..........

1681 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag 1740

1681 agtcagcttt ctgacccgga aagcaaaata gacaacaaac agccacttgc gagaggactc 1740

.... rrnB_term rrnB_T1_term .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg 1800

1741 atcctgttta ggcggccctc gcctaaactt gcaacgcttc gttgccgggc ctcccaccgc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga 1860

1801 ccgtcctgcg ggcggtattt gacggtccgt agtttaattc gtcttccggt aggactgcct 1860

.......... .......... .......... .......... ......... rrnB_T2_term

.......... .......... .......... .......... .......... ..........

1861 tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt 1920

1861 accggaaaaa cgcaaagatg tttgagaaaa caaataaaaa gatttatgta agtttataca 1920

rrnB_T2_term ........ .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

1921 atccgctcat gagacaataa ccctagctta ctggccgtcg ttttacaacg tcgtgactgg 1980

1921 taggcgagta ctctgttatt gggatcgaat gaccggcagc aaaatgttgc agcactgacc 1980

AmpR_pro .......... M13_forward20_pri M13_pUC_fwd_pri lacZ_a

.......... .......... .......... .......... .......... ..........

1981 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 2040

1981 cttttgggac cgcaatgggt tgaattagcg gaacgtcgtg tagggggaaa gcggtcgacc 2040

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... FspI(2086) ..........

2041 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 2100

2041 gcattatcgc ttctccgggc gtggctagcg ggaagggttg tcaacgcgtc ggacttaccg 2100

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 2160

2101 cttaccgcgg actacgccat aaaagaggaa tgcgtagaca cgccataaag tgtggcgtat 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg 2220

2161 accacgtgag agtcatgtta gacgagacta cggcgtatca attcggtcgg ggctgtgggc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccaacacccg ctgacgagct tagtaaagcc ctcgctagat tttaatgcgg atgttgcgat 2280

2221 ggttgtgggc gactgctcga atcatttcgg gagcgatcta aaattacgcc tacaacgcta 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tacttcgcca actattgcga taacaagaaa aagccagcct ttcatgatat atctcccaat 2340

2281 atgaagcggt tgataacgct attgttcttt ttcggtcgga aagtactata tagagggtta 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac ctgatagttt 2400

2341 aacacatccc gaataatacg tgcgaatttt tattattttc gtctgaactg gactatcaaa 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggctgtgagc aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc 2460

2401 ccgacactcg ttaatacacg aatcacgtag attgcgaact caattcggcg cggcgcttcg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggcgtcggct tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt 2520

2461 ccgcagccga acttgcttaa caatctgtaa taaacggctg atggaaccac tagagcggaa 2520

.......... .......... .......... ......... ORF 2 spect .........

.......... .......... .......... .......... .......... ..........

2521 tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt 2580

2521 agtgcatcac ctgtttaaga aggttgacta gacgcgcgct ccggttcgct agaagaagaa 2580

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtccaagata agcctgtcta gcttcacgta tgacgggctg atactgggcc ggcaggcgct 2640

2581 caggttctat tcggacagat cgaagtgcat actgcccgac tatgacccgg ccgtccgcga 2640

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccattgccca gtcggcagcg acctccttcg gcgcgatttt gccggttact gcgctgtacc 2700

2641 ggtaacgggt cagccgtcgc tggaggaagc cgcgctaaaa cggccaatga cgcgacatgg 2700

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt 2760

2701 tttacgccct gttgcattcg tgatgtaaag cgagtagcgg tcgggtcagc ccgccgctca 2760

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa 2820

2761 aggtatcgca attccaaagt aaatcgcgga gtttatctag gacaagtcct tggcctagtt 2820

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca 2880

2821 tctcaaggag gcggcgacct ggatggttcc gttgcgatac aagagaacga aaacagtcgt 2880

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc 2940

2881 tctatcggtc tagttacagc tagcaccgac cgagcttcta tggacgttct tacagtaacg 2940

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt 3000

2941 cgacggtaag aggtttaacg tcaagcgcga atcgacctat tgcggtgcct tactacagca 3000

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg 3060

3001 gcacgtgttg ttaccactga agatgtcgcg cctcttagag cgagagaggt ccccttcggc 3060

.......... ......... ORF 2 spect ......... .......... ..........

.......... BclI(3079) .......... .......... .......... ..........

3061 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca 3120

3061 ttcaaaggtt ttccagcaac tagtttcgag cggcgcaaca aagtagttcg gaatgccagt 3120

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt 3180

3121 ggcattggtc gtttagttat agtgacacac cgaagtccgg cggtaggtga cgcctcggca 3180

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg 3240

3181 tgtttacatg ccggtcgttg cagccaagct ctaccgcgag ctactgcggt tgatggagac 3240

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 atagttgagt cgatacttcg gcgatcaccg cttccctcat gatgtttaac tttgttttag 3300

3241 tatcaactca gctatgaagc cgctagtggc gaagggagta ctacaaattg aaacaaaatc 3300

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg 3360

3301 ccgctgacgg gacgacgcat tgtagcaacg acgaggtatt gtagtttgta gctgggtgcc 3360

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacagtcat 3420

3361 gcattgcgcg aacgacgaac ctacgggctc cgtatctgac atggggtttt tttgtcagta 3420

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aacaagccat gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg 3480

3421 ttgttcggta cttttggcgg tgacgcggca atggtggcga cgcaagccag ttccaagacc 3480

.......... ......... ORF 2 spect ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 accagttgcg tgagcgcata cgctacttgc attacagctt acgaaccgaa caggcttatg 3540

3481 tggtcaacgc actcgcgtat gcgatgaacg taatgtcgaa tgcttggctt gtccgaatac 3540

ORF 2 spect ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tccactgggt tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca 3600

3541 aggtgaccca agcacggaag taggcaaagg tgccacacgc agtgggccgt tggaacccgt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gcagcgaagt cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca 3660

3601 cgtcgcttca gctccgtaaa gacaggaccg accgcttgct cgcgttccaa agccagaggt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgcatcgtca ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc 3720

3661 gcgtagcagt ccgtaaccgc cggaacgaca agaagatgcc gttccacgac acgtgcctag 3720

.......... .......... .......... .......... .......... ..........

.......... .......... EagI(3750) .......... .......... ..........

3721 tgccctggct tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga 3780

3721 acgggaccga agtcctctag ccttctggag ccggcagcgc cgcgaacggc caccacgact 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccccggatga agtaattccc acgggttttg ctgcccgcaa acgggctgtt ctggtgttgc 3840

3781 ggggcctact tcattaaggg tgcccaaaac gacgggcgtt tgcccgacaa gaccacaacg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tagtttgtta tcagaatcgc agatccggct tcaggtttgc cggctgaaag cgctatttct 3900

3841 atcaaacaat agtcttagcg tctaggccga agtccaaacg gccgactttc gcgataaaga 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca 3960

3901 aggtcttaac ggtactaaaa aaggggtgcc ctccgcagtg accgagggca caacagccgt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg 4020

3961 cgaaactaag ctattcgtcg tagcggacaa agtccgacag atacacactg acaactcgac 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac 4080

4021 attgttcaac agagtccaca agttaaagta caagatcaac gaaacaaaat gaccaaagtg 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa 4140

4081 gacaagataa tccacaatgt acgacaagta gacaatgtaa cagctagaca agtaccactt 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt 4200

4141 gtcgaaactt acgtggtttt tgagcatttt cgagactaca tagatagaaa aaatgtggca 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac 4260

4201 aaagtagaca cgtatacctg tcaaaaggga aactatacat tgccacttgt caacaagatg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc 4320

4261 aaaacaaaca atcagaacta cgaagtgact atctatgttc tcggtattct tggagtctag 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag 4380

4321 gaaggcataa atcggtcata caagagatca caccaagcaa caaaaacgca ctcggtactc 4380

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg 4440

4381 ttgcttggta actctagtat gaatgaaacg tacagtgagt ttttaaaacg gagttttgac 4440

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg 4500

4441 cactcgactt aaaaacgtca atttcgtagc acatcacaaa aagaatcagg caatacatcc 4500

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc 4560

4501 atccttagac tacattacca acaaccataa aacagtggta agtaaaaata gaccaacaag 4560

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca 4620

4561 agttcaagcc aatgctctag gtaaacagat agatcaagtt gaacctttta gttgcatagt 4620

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta 4680

4621 cagcccgccg gagcgaatag ttggtggtta aagtataacg acattcacaa atttagaaat 4680

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc 4740

4681 gaataaccaa agttttgggt aaccaattcg gaaaatttga gtaccatcaa taaaagttcg 4740

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt 4800

4741 taattgtact tgaatttaag tagttccgat tagagatata aacggaacac tcaaaagaaa 4800

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac 4860

4801 acacaatcaa gaaaattatt ggtgagtatt taggagtatc tcataaacaa aagttttctg 4860

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc 4920

4861 aattgtacaa ggtctaatat aaaatactta aaaaaattga ccttttctat tccgttatag 4920

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg 4980

4921 agaagtgatt tttgattaag attaaaaagc gaactcttga accgtatcaa acaggtgacc 4980

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct 5040

4981 ttttagagtt tcggaaattg gtttcctaag gactaaaggt gtcaagagca gtagtcgaga 5040

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg 5100

5041 gaccaacgaa atcgattatg tggtattcgt aaaagggatg actacaagta gtagactcgc 5100

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg 5160

5101 ataaccaata ttcacttgct atggcaggca agaaaggaac atcccaaaag ttagcacccc 5160

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga 5220

5161 aactcatcac ggtgtgtcgt attttaatcg aaccaaagta cgaggcaatt cagtatcgct 5220

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct 5280

5221 gattagcgat caagtaaacg aaacttttgt tgattaagtc tgtatgtaga gttaaccaga 5280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... SpeI(5331)

5281 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt 5340

5281 tccactaaaa ttagtgatat ggttaactct acccgatcag ttactattaa tgatcaggaa 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ttcctttgag ttgtgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat 5400

5341 aaggaaactc aacacccata gacatttaag acgatctgga aacgaccttt tgaacattta 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa 5460

5401 agacgatctg ggagacattt aaggcgatct ggaaacacac aaaaaaaaca aatataagtt 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc 5520

5461 caccaatatt aaatatctta tttctttctt atttttttct atttttctta tctagggtcg 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg 5580

5521 ggacacatat tgagtgatga aatcagtcaa ggcgtcataa tgttttccta cagcgtttgc 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca 5640

5581 gacaaacgag gagatgtttt gtctggaatt ttgggatttc cgaattcatc gtgggagcgt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg 5700

5641 tcgagcccgt ttagcgactt ataaggaaaa cagaggctgg tagtccgtgg actcagcgac 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg 5760

5701 agaaaaagca ctgtaagtca agcgacgcga gtgccgagac cgtcacttac ccccatttac 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc 5820

5761 cgtgatgtcc gcggaaaata cctaagtacg ttcctttgat gggtattatg ttcttttcgg 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt 5880

5821 gcagtgcccg aagagtcccg caaaataccg cccagacgat acaccacgat agactgaaaa 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg 5940

5881 acgacaagtc gtcaaggacg ggagactaaa aggtcagact ggtgaagcct aatagggcac 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac 6000

5941 tgtccagtaa gtctgaccga ttacgtgggt cattccgtcg ccatagtagt tgtccgaatg 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ccgtcttact gtcgggaatt aattcgttgg ccgattcatt aatgcagctg gcacgacagg 6060

6001 ggcagaatga cagcccttaa ttaagcaacc ggctaagtaa ttacgtcgac cgtgctgtcc 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 6120

6061 aaagggctga cctttcgccc gtcactcgcg ttgcgttaat tacactcaat cgagtgagta 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgag. 6179

6121 atccgtgggg tccgaaatgt gaaatacgaa ggccgagcat acaacacacc ttaacactc. 6179

.......... .......... lac_pro .......... .......... ..........