Vh"Vh"&LOCUS pBAD44 5466 bp DNA SYN 20-Sep-2007 DEFINITION pBAD44 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5466 /organism="pBAD44" /mol_type="other DNA" misc_feature complement(96..974) /label="AraC" CDS complement(96..1025) /label="ORF frame 3" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 1004..1019 /label="araO2" misc_feature 1161..1172 /label="araO1" misc_feature 1203..1216 /label="CAP_BS" misc_feature 1208..1227 /label="pBAD_fwd_primer" misc_feature 1213..1251 /label="AraI1I2" promoter 1248..1276 /label="ARA_promoter" misc_feature complement(1395..1412) /label="pBAD_rev_primer" misc_feature complement(1395..1412) /label="pTrcHis_rev_primer" terminator 1445..1602 /label="rrnB_terminator" terminator 1568..1611 /label="rrnB_T1_terminator" terminator 1743..1770 /label="rrnB_T2_terminator" promoter 1811..1839 /label="AmpR_promoter" CDS complement(2285..2944) /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 2" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene complement(2285..2944) /label="CAT/CamR" /gene="CAT/CamR" CDS 3815..4765 /label="ORF frame 2" /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI*" promoter 5327..5356 /label="lac_promoter" ORIGIN 1 ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC 61 TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA 121 TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA 181 AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA 241 GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG 301 CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG 361 CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG 421 TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT 481 TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC 541 CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC 601 GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA 661 TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA 721 TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA 781 ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA 841 TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC 901 GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT 961 TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT 1021 TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA 1081 ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT 1141 AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA 1201 CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG ATCCTACCTG ACGCTTTTTA 1261 TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCGAATT CGAGCTCGGT 1321 ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTGGCTGTTT TGGCGGATGA 1381 GAGAAGATTT TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG 1441 AATTTGCCTG GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG 1501 AAACGCCGTA GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG 1561 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT 1621 GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA GCGGATTTGA ACGTTGCGAA 1681 GCAACGGCCC GGAGGGTGGC GGGCAGGACG CCCGCCATAA ACTGCCAGGC ATCAAATTAA 1741 GCAGAAGGCC ATCCTGACGG ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT 1801 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTAGCTT GACTGGGAAA 1861 ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA 1921 ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT 1981 GGCGCTAACC GTTTTTATCA GGCTCTGGGA GGCAGAATAA ATGATCATAT CGTCAATTAT 2041 TACCTCCACG GGGAGAGCCT GAGCAAACTG GCCTCAGGCA TTTGAGAAGC ACACGGTCAC 2101 ACTGCTTCCG GTAGTCAATA AACCGGTAAA CCAGCAATAG ACATAAGCGG CTATTTAACG 2161 ACCCTGCCCT GAACCGACGA CCGGGTCGAA TTTGCTTTCG AATTTCTGCC ATTCATCCGC 2221 TTATTATCAC TTATTCAGGC GTAGCACCAG GCGTTTAAGG GCACCAATAA CTGCCTTAAA 2281 AAAATTACGC CCCGCCCTGC CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC 2341 CGACATGGAA GCCATCACAG ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT 2401 TGTCGCCTTG CGTATAATAT TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT 2461 TGGCCACGTT TAAATCAAAA CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA 2521 TATTCTCAAT AAACCCTTTA GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT 2581 GCGAATATAT GTGTAGAAAC TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA 2641 ACGTTTCAGT TTGCTCATGG AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA 2701 GCTCACCGTC TTTCATTGCC ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA 2761 TGTGAATAAA GGCCGGATAA AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG 2821 TAATATCCAG CTGAACGGTC TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA 2881 AATGTTCTTT ACGATGCCAT TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT 2941 CCATTTTAGC TTCCTTAGCT CCTGAAAATC TCGATAACTC AAAAAATACG CCCGGTAGTG 3001 ATCTTATTTC ATTATGGTGA AAGTTGGAAC CTCTTACGTG CCGATCAACG TCTCATTTTC 3061 GCCAAAAGTT GGCCCAGGGC TTCCCGGTAT CAACAGGGAC ACCAGGATTT ATTTATTCTG 3121 CGAAGTGATC TTCCGTCACA GGTATTTATT CGGCGCAAAG TGCGTCGGGT GATGCTGCCA 3181 ACTTACTGAT TTAGTGTATG ATGGTGTTTT TGAGGTGCTC CAGTGGCTTC TGTTTCTATC 3241 AGCTGTCCCT CCTGTTCAGC TACTGACGGG GTGGTGCGTA ACGGCAAAAG CACCGCCGGA 3301 CATCAGCGCC TGTAGTGCCA TTTACCCCCA TTCACTGCCA GAGCCGTGAG CGCAGCGAAC 3361 TGAATGTCAC GAAAAAGACA GCGACTCAGG TGCCTGATGG TCGGAGACAA AAGGAATATT 3421 CAGCGATTTG CCCGAGCTTG CGAGGGTGCT ACTTAAGCCT TTAGGGTTTT AAGGTCTGTT 3481 TTGTAGAGGA GCAAACAGCG TTTGCGACAT CCTTTTGTAA TACTGCGGAA CTGACTAAAG 3541 TAGTGAGTTA TACACAGGGC TGGGATCTAT TCTTTTTATC TTTTTTTATT CTTTCTTTAT 3601 TCTATAAATT ATAACCACTT GAATATAAAC AAAAAAAACA CACAAAGGTC TAGCGGAATT 3661 TACAGAGGGT CTAGCAGAAT TTACAAGTTT TCCAGCAAAG GTCTAGCAGA ATTTACAGAT 3721 ACCCACAACT CAAAGGAAAA GGACTAGTAA TTATCATTGA CTAGCCCATC TCAATTGGTA 3781 TAGTGATTAA AATCACCTAG ACCAATTGAG ATGTATGTCT GAATTAGTTG TTTTCAAAGC 3841 AAATGAACTA GCGATTAGTC GCTATGACTT AACGGAGCAT GAAACCAAGC TAATTTTATG 3901 CTGTGTGGCA CTACTCAACC CCACGATTGA AAACCCTACA AGGAAAGAAC GGACGGTATC 3961 GTTCACTTAT AACCAATACG CTCAGATGAT GAACATCAGT AGGGAAAATG CTTATGGTGT 4021 ATTAGCTAAA GCAACCAGAG AGCTGATGAC GAGAACTGTG GAAATCAGGA ATCCTTTGGT 4081 TAAAGGCTTT GAGATTTTCC AGTGGACAAA CTATGCCAAG TTCTCAAGCG AAAAATTAGA 4141 ATTAGTTTTT AGTGAAGAGA TATTGCCTTA TCTTTTCCAG TTAAAAAAAT TCATAAAATA 4201 TAATCTGGAA CATGTTAAGT CTTTTGAAAA CAAATACTCT ATGAGGATTT ATGAGTGGTT 4261 ATTAAAAGAA CTAACACAAA AGAAAACTCA CAAGGCAAAT ATAGAGATTA GCCTTGATGA 4321 ATTTAAGTTC ATGTTAATGC TTGAAAATAA CTACCATGAG TTTAAAAGGC TTAACCAATG 4381 GGTTTTGAAA CCAATAAGTA AAGATTTAAA CACTTACAGC AATATGAAAT TGGTGGTTGA 4441 TAAGCGAGGC CGCCCGACTG ATACGTTGAT TTTCCAAGTT GAACTAGATA GACAAATGGA 4501 TCTCGTAACC GAACTTGAGA ACAACCAGAT AAAAATGAAT GGTGACAAAA TACCAACAAC 4561 CATTACATCA GATTCCTACC TACGTAACGG ACTAAGAAAA ACACTACACG ATGCTTTAAC 4621 TGCAAAAATT CAGCTCACCA GTTTTGAGGC AAAATTTTTG AGTGACATGC AAAGTAAGCA 4681 TGATCTCAAT GGTTCGTTCT CATGGCTCAC GCAAAAACAA CGAACCACAC TAGAGAACAT 4741 ACTGGCTAAA TACGGAAGGA TCTGAGGTTC TTATGGCTCT TGTATCTATC AGTGAAGCAT 4801 CAAGACTAAC AAACAAAAGT AGAACAACTG TTCACCGTTA GATATCAAAG GGAAAACTGT 4861 CCATATGCAC AGATGAAAAC GGTGTAAAAA AGATAGATAC ATCAGAGCTT TTACGAGTTT 4921 TTGGTGCATT TAAAGCTGTT CACCATGAAC AGATCGACAA TGTAACAGAT GAACAGCATG 4981 TAACACCTAA TAGAACAGGT GAAACCAGTA AAACAAAGCA ACTAGAACAT GAAATTGAAC 5041 ACCTGAGACA ACTTGTTACA GCTCAACAGT CACACATAGA CAGCCTGAAA CAGGCGATGC 5101 TGCTTATCGA ATCAAAGCTG CCGACAACAC GGGAGCCAGT GACGCCTCCC GTGGGGAAAA 5161 AATCATGGCA ATTCTGGAAG AAATAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT 5221 GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC 5281 GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC CCAGGCTTTA CACTTTATGC 5341 TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCCTGATATA GGCGCCAGCA ACCGCACCTG 5401 TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCTGCTCA TGTTTGACAG 5461 CTTATC //ZpBAD44 "Times New RomanVh"CZ`AraC"Times New RomanVh"CZ`ORF 3"Times New RomanVh"CɆZaraO2"Times New RomanVh"ZaraO1"Times New RomanVh"CZCAP_BS"Times New RomanVh"CZ pBAD_fwd_pri"Times New RomanVh"CɆZAraI1I2"Times New Roman*Vh"ZARA_pro"Times New RomanVh"CZs pBAD_rev_pri"Times New RomanVh"CZspTrcHis_rev_pri"Times New RomanVh"CɆZB rrnB_term"Times New RomanVh"Z K rrnB_T1_term"Times New RomanVh"CZ rrnB_T2_term"Times New RomanVh"CZ/AmpR_pro"Times New RomanVh"CɆZ ORF 2"Times New RomanVh"Z CAT/CamR"Times New RomanVh"CZORF 2"Times New RomanVh"CZlac_pro"Times New Roman Vh"ClaI"Times New RomanZVB.n. Vh"NruI"Times New RomanZ'5CCq{ Vh"fNheI(1300),EcoRI(1306),SacI(1316),KpnI(1322),SmaI(1324),XbaI(1333),SalI(1339),PstI(1349),HindIII(1357)"Times New RomanZg'{&$g Vh"FspI(1962),BclI(2022)"Times New RomanZ4DSS Vh"BstBI"Times New RomanZ c% Vh"NcoI(2425),MscI(2463)"Times New Romany Z%44-R Vh"SpeI"Times New RomanZL Vh"NdeI"Times New RomanZ|l]l Vh"test"Times New RomanZgDWDH@H