Sequence of pBAD44

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Sequence of pBAD44

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Sequence of pBAD44 : 5466 BP; 1595 A; 1279 C; 1221 G; 1371 T; 0 other;

ClaI(2) .......... .......... .......... .......... ..........

1 atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 60

1 tagctacgta ttacacggac agtttacctg cttcgtccct aagacgtttg ggatacgatg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 120

61 aggcagttcg gcagttaaca gactaagcaa tggttaatac tgttgaactg ccgatgtagt 120

.......... .......... .......... .......... AraC ORF 3 ..........

.......... .......... .......... .......... .......... ..........

121 ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 180

121 aagtgaaaaa gaagtgttgg ccgtgccttg agcgagcccg accggggcca cgtaaaaaat 180

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 240

181 ttatgggcgc tctttatctc aactagcagt tttggttgta acgctggctg ccaccgctat 240

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 300

241 ccgtaggccc accacgagtt ttcgtcgaag cggaccgact atgcaaccag gagcgcggtc 300

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 360

301 gaattctgcg attagggatt gacgaccgcc ttttctacac tgtctgcgct gccgctgttc 360

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 420

361 gtttgtacga cacgctgcga ccgctatagt tttaacgaca gacggtccac tagcgactac 420

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 480

421 atgactgttc ggagcgcatg ggctaatagg tagccaccta cctcgctgag caattagcga 480

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 540

481 aggtacgcgg cgtcattgtt aacgagttcg tctaaatagc ggtcgtcgag gcttatcgcg 540

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 600

541 ggaaggggaa cgggccgcaa ttactaaacg ggtttgtcca gcgactttac gccgaccacg 600

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 660

601 cgaagtaggc ccgctttctt ggggcataac cgtttataac tgccggtcaa ttcggtaagt 660

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... NruI(708) ..........

661 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 720

661 acggtcatcc gcgcgcctgc tttcatttgg gtgaccacta tggtaagcgc tcggaggcct 720

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 780

721 actgctggca tcactactta gagaggaccg cccttgtcgt tttatagtgg gccagccgtt 780

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 840

781 tgtttaagag cagggactaa aaagtggtgg gggactggcg cttaccactc taactcttat 840

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 900

841 attggaaagt aagggtcgcc agccagctat ttttttagct ctattggcaa ccggagttag 900

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 960

901 ccgcaatttg ggcggtggtc tacccgtaat ttgctcatag ggccgtcgtc ccctagtaaa 960

.......... .......... AraC ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 1020

961 acgcgaagtc ggtatgaaaa gtatgagggc ggtaagtctc ttctttggtt aacaggtata 1020

AraC .......... ORF 3 .......... .......... araO2

.......... .......... .......... .......... .......... ..........

1021 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 1080

1021 acgtagtctg taacggcagt gacgcagaaa atgaccgaga agagcgattg gtttggccat 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 1140

1081 tggggcgaat aattttcgta agacattgtt tcgccctggt ttcggtactg tttttgcgca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 1200

1141 ttgttttcac agatattagt gccgtctttt caggtgtaac taataaacgt gccgcagtgt 1200

.......... .......... araO1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 1260

1201 gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc taggatggac tgcgaaaaat 1260

CAP_BS pBAD_fwd_pri ........ AraI1I2 .......... ARA_pro

.......... .......... ......... NheI(1300) EcoRI(1306) .........

1261 tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcgaatt cgagctcggt 1320

1261 agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc gatcgcttaa gctcgagcca 1320

ARA_pro .......... .......... .......... .......... ..........

KpnI,SmaI XbaI SalI1339) PstI(1349) HindIII(1357)

1321 acccggggat cctctagagt cgacctgcag gcatgcaagc ttggctgttt tggcggatga 1380

1321 tgggccccta ggagatctca gctggacgtc cgtacgttcg aaccgacaaa accgcctact 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag 1440

1381 ctcttctaaa agtcggacta tgtctaattt agtcttgcgt cttcgccaga ctattttgtc 1440

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg 1500

1441 ttaaacggac cgccgtcatc gcgccaccag ggtggactgg ggtacggctt gagtcttcac 1500

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag 1560

1501 tttgcggcat cgcggctacc atcacacccc agaggggtac gctctcatcc cttgacggtc 1560

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1620

1561 cgtagtttat tttgctttcc gagtcagctt tctgacccgg aaagcaaaat agacaacaaa 1620

.......... .... rrnB_term rrnB_T1_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa 1680

1621 cagccacttg cgagaggact catcctgttt aggcggccct cgcctaaact tgcaacgctt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa 1740

1681 cgttgccggg cctcccaccg cccgtcctgc gggcggtatt tgacggtccg tagtttaatt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt 1800

1741 cgtcttccgg taggactgcc taccggaaaa acgcaaagat gtttgagaaa acaaataaaa 1800

......... rrnB_T2_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tctaaataca ttcaaatatg tatccgctca tgagacaata accctagctt gactgggaaa 1860

1801 agatttatgt aagtttatac ataggcgagt actctgttat tgggatcgaa ctgacccttt 1860

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 1920

1861 tgggaccgca atgggttgaa ttagcggaac gtcgtgtagg gggaaagcgg tcgaccgcat 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 1980

1921 tatcgcttct ccgggcgtgg ctagcgggaa gggttgtcaa cgcgtcggac ttaccgctta 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... BclI(2022) ..........

1981 ggcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat 2040

1981 ccgcgattgg caaaaatagt ccgagaccct ccgtcttatt tactagtata gcagttaata 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac 2100

2041 atggaggtgc ccctctcgga ctcgtttgac cggagtccgt aaactcttcg tgtgccagtg 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg 2160

2101 tgacgaaggc catcagttat ttggccattt ggtcgttatc tgtattcgcc gataaattgc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... ......... BstBI(2198) ......... ..........

2161 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc 2220

2161 tgggacggga cttggctgct ggcccagctt aaacgaaagc ttaaagacgg taagtaggcg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa 2280

2221 aataatagtg aataagtccg catcgtggtc cgcaaattcc cgtggttatt gacggaattt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc 2340

2281 ttttaatgcg gggcgggacg gtgagtagcg tcatgacaac attaagtaat tcgtaagacg 2340

.......... .......... ........ ORF 2 CAT/CamR ........ ..........

.......... .......... .......... .......... .......... ..........

2341 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct 2400

2341 gctgtacctt cggtagtgtc tgccgtacta cttggactta gcggtcgccg tagtcgtgga 2400

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat 2460

2401 acagcggaac gcatattata aacgggtacc acttttgccc ccgcttcttc aacaggtata 2460

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

MscI(2463) .......... .......... .......... .......... ..........

2461 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca 2520

2461 accggtgcaa atttagtttt gaccactttg agtgggtccc taaccgactc tgctttttgt 2520

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt 2580

2521 ataagagtta tttgggaaat ccctttatcc ggtccaaaag tggcattgtg cggtgtagaa 2580

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa 2640

2581 cgcttatata cacatctttg acggccttta gcagcaccat aagtgaggtc tcgctacttt 2640

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca 2700

2641 tgcaaagtca aacgagtacc ttttgccaca ttgttcccac ttgtgatagg gtatagtggt 2700

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gctcaccgtc tttcattgcc atacgaaatt ccggatgagc attcatcagg cgggcaagaa 2760

2701 cgagtggcag aaagtaacgg tatgctttaa ggcctactcg taagtagtcc gcccgttctt 2760

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg 2820

2761 acacttattt ccggcctatt ttgaacacga ataaaaagaa atgccagaaa tttttccggc 2820

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa 2880

2821 attataggtc gacttgccag accaatatcc atgtaactcg ttgactgact ttacggagtt 2880

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct 2940

2881 ttacaagaaa tgctacggta accctatata gttgccacca tataggtcac taaaaaaaga 2940

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg 3000

2941 ggtaaaatcg aaggaatcga ggacttttag agctattgag ttttttatgc gggccatcac 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc 3060

3001 tagaataaag taataccact ttcaaccttg gagaatgcac ggctagttgc agagtaaaag 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt atttattctg 3120

3061 cggttttcaa ccgggtcccg aagggccata gttgtccctg tggtcctaaa taaataagac 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca 3180

3121 gcttcactag aaggcagtgt ccataaataa gccgcgtttc acgcagccca ctacgacggt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc 3240

3181 tgaatgacta aatcacatac taccacaaaa actccacgag gtcaccgaag acaaagatag 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga 3300

3241 tcgacaggga ggacaagtcg atgactgccc caccacgcat tgccgttttc gtggcggcct 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 catcagcgcc tgtagtgcca tttaccccca ttcactgcca gagccgtgag cgcagcgaac 3360

3301 gtagtcgcgg acatcacggt aaatgggggt aagtgacggt ctcggcactc gcgtcgcttg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgaatgtcac gaaaaagaca gcgactcagg tgcctgatgg tcggagacaa aaggaatatt 3420

3361 acttacagtg ctttttctgt cgctgagtcc acggactacc agcctctgtt ttccttataa 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cagcgatttg cccgagcttg cgagggtgct acttaagcct ttagggtttt aaggtctgtt 3480

3421 gtcgctaaac gggctcgaac gctcccacga tgaattcgga aatcccaaaa ttccagacaa 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttgtagagga gcaaacagcg tttgcgacat ccttttgtaa tactgcggaa ctgactaaag 3540

3481 aacatctcct cgtttgtcgc aaacgctgta ggaaaacatt atgacgcctt gactgatttc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tagtgagtta tacacagggc tgggatctat tctttttatc tttttttatt ctttctttat 3600

3541 atcactcaat atgtgtcccg accctagata agaaaaatag aaaaaaataa gaaagaaata 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tctataaatt ataaccactt gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt 3660

3601 agatatttaa tattggtgaa cttatatttg ttttttttgt gtgtttccag atcgccttaa 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tacagagggt ctagcagaat ttacaagttt tccagcaaag gtctagcaga atttacagat 3720

3661 atgtctccca gatcgtctta aatgttcaaa aggtcgtttc cagatcgtct taaatgtcta 3720

.......... .......... .......... .......... .......... ..........

.......... .......... SpeI(3743) .......... .......... ..........

3721 acccacaact caaaggaaaa ggactagtaa ttatcattga ctagcccatc tcaattggta 3780

3721 tgggtgttga gtttcctttt cctgatcatt aatagtaact gatcgggtag agttaaccat 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tagtgattaa aatcacctag accaattgag atgtatgtct gaattagttg ttttcaaagc 3840

3781 atcactaatt ttagtggatc tggttaactc tacatacaga cttaatcaac aaaagtttcg 3840

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

3841 aaatgaacta gcgattagtc gctatgactt aacggagcat gaaaccaagc taattttatg 3900

3841 tttacttgat cgctaatcag cgatactgaa ttgcctcgta ctttggttcg attaaaatac 3900

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ctgtgtggca ctactcaacc ccacgattga aaaccctaca aggaaagaac ggacggtatc 3960

3901 gacacaccgt gatgagttgg ggtgctaact tttgggatgt tcctttcttg cctgccatag 3960

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gttcacttat aaccaatacg ctcagatgat gaacatcagt agggaaaatg cttatggtgt 4020

3961 caagtgaata ttggttatgc gagtctacta cttgtagtca tcccttttac gaataccaca 4020

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 attagctaaa gcaaccagag agctgatgac gagaactgtg gaaatcagga atcctttggt 4080

4021 taatcgattt cgttggtctc tcgactactg ctcttgacac ctttagtcct taggaaacca 4080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 taaaggcttt gagattttcc agtggacaaa ctatgccaag ttctcaagcg aaaaattaga 4140

4081 atttccgaaa ctctaaaagg tcacctgttt gatacggttc aagagttcgc tttttaatct 4140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 attagttttt agtgaagaga tattgcctta tcttttccag ttaaaaaaat tcataaaata 4200

4141 taatcaaaaa tcacttctct ataacggaat agaaaaggtc aattttttta agtattttat 4200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 taatctggaa catgttaagt cttttgaaaa caaatactct atgaggattt atgagtggtt 4260

4201 attagacctt gtacaattca gaaaactttt gtttatgaga tactcctaaa tactcaccaa 4260

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 attaaaagaa ctaacacaaa agaaaactca caaggcaaat atagagatta gccttgatga 4320

4261 taattttctt gattgtgttt tcttttgagt gttccgttta tatctctaat cggaactact 4320

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 atttaagttc atgttaatgc ttgaaaataa ctaccatgag tttaaaaggc ttaaccaatg 4380

4321 taaattcaag tacaattacg aacttttatt gatggtactc aaattttccg aattggttac 4380

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ggttttgaaa ccaataagta aagatttaaa cacttacagc aatatgaaat tggtggttga 4440

4381 ccaaaacttt ggttattcat ttctaaattt gtgaatgtcg ttatacttta accaccaact 4440

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 taagcgaggc cgcccgactg atacgttgat tttccaagtt gaactagata gacaaatgga 4500

4441 attcgctccg gcgggctgac tatgcaacta aaaggttcaa cttgatctat ctgtttacct 4500

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tctcgtaacc gaacttgaga acaaccagat aaaaatgaat ggtgacaaaa taccaacaac 4560

4501 agagcattgg cttgaactct tgttggtcta tttttactta ccactgtttt atggttgttg 4560

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cattacatca gattcctacc tacgtaacgg actaagaaaa acactacacg atgctttaac 4620

4561 gtaatgtagt ctaaggatgg atgcattgcc tgattctttt tgtgatgtgc tacgaaattg 4620

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgcaaaaatt cagctcacca gttttgaggc aaaatttttg agtgacatgc aaagtaagca 4680

4621 acgtttttaa gtcgagtggt caaaactccg ttttaaaaac tcactgtacg tttcattcgt 4680

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgatctcaat ggttcgttct catggctcac gcaaaaacaa cgaaccacac tagagaacat 4740

4681 actagagtta ccaagcaaga gtaccgagtg cgtttttgtt gcttggtgtg atctcttgta 4740

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 actggctaaa tacggaagga tctgaggttc ttatggctct tgtatctatc agtgaagcat 4800

4741 tgaccgattt atgccttcct agactccaag aataccgaga acatagatag tcacttcgta 4800

.......... ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 caagactaac aaacaaaagt agaacaactg ttcaccgtta gatatcaaag ggaaaactgt 4860

4801 gttctgattg tttgttttca tcttgttgac aagtggcaat ctatagtttc ccttttgaca 4860

.......... .......... .......... .......... .......... ..........

NdeI(4863) .......... .......... .......... .......... ..........

4861 ccatatgcac agatgaaaac ggtgtaaaaa agatagatac atcagagctt ttacgagttt 4920

4861 ggtatacgtg tctacttttg ccacattttt tctatctatg tagtctcgaa aatgctcaaa 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ttggtgcatt taaagctgtt caccatgaac agatcgacaa tgtaacagat gaacagcatg 4980

4921 aaccacgtaa atttcgacaa gtggtacttg tctagctgtt acattgtcta cttgtcgtac 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 taacacctaa tagaacaggt gaaaccagta aaacaaagca actagaacat gaaattgaac 5040

4981 attgtggatt atcttgtcca ctttggtcat tttgtttcgt tgatcttgta ctttaacttg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 acctgagaca acttgttaca gctcaacagt cacacataga cagcctgaaa caggcgatgc 5100

5041 tggactctgt tgaacaatgt cgagttgtca gtgtgtatct gtcggacttt gtccgctacg 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tgcttatcga atcaaagctg ccgacaacac gggagccagt gacgcctccc gtggggaaaa 5160

5101 acgaatagct tagtttcgac ggctgttgtg ccctcggtca ctgcggaggg cacccctttt 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aatcatggca attctggaag aaatagcgcc caatacgcaa accgcctctc cccgcgcgtt 5220

5161 ttagtaccgt taagaccttc tttatcgcgg gttatgcgtt tggcggagag gggcgcgcaa 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 5280

5221 ccggctaagt aattacgtcg accgtgctgt ccaaagggct gacctttcgc ccgtcactcg 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 5340

5281 cgttgcgtta attacactca atcgagtgag taatccgtgg ggtccgaaat gtgaaatacg 5340

.......... .......... .......... .......... .......... lac_pro

.......... .......... .......... .......... .......... ..........

5341 ttccggctcg tatgttgtgt ggaattgtga gcctgatata ggcgccagca accgcacctg 5400

5341 aaggccgagc atacaacaca ccttaacact cggactatat ccgcggtcgt tggcgtggac 5400

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctgctca tgtttgacag 5460

5401 accgcggcca ctacggccgg tgctacgcag gccgcatctc ctagacgagt acaaactgtc 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cttatc.... .......... .......... .......... .......... .......... 5466

5461 gaatag.... .......... .......... .......... .......... .......... 5466

.......... .......... .......... .......... .......... ..........