S}S}&LOCUS pBAD45 5470 bp DNA SYN 02-Sep-2007 DEFINITION pBAD45 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5470 /organism="pBAD45" /mol_type="other DNA" misc_feature complement(96..974) /label="AraC" CDS complement(96..1025) /label="ORF frame 3" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 1004..1019 /label="araO2" misc_feature 1161..1172 /label="araO1" misc_feature 1203..1216 /label="CAP_BS" misc_feature 1208..1227 /label="pBAD_fwd_primer" misc_feature 1213..1251 /label="AraI1I2" promoter 1248..1276 /label="ARA_promoter" misc_feature complement(1399..1416) /label="pBAD_rev_primer" misc_feature complement(1399..1416) /label="pTrcHis_rev_primer" terminator 1449..1606 /label="rrnB_terminator" terminator 1572..1615 /label="rrnB_T1_terminator" terminator 1747..1774 /label="rrnB_T2_terminator" promoter 1815..1843 /label="AmpR_promoter" CDS complement(2289..2948) /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 3" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene complement(2289..2948) /label="CAT/CamR" /gene="CAT/CamR" CDS 3819..4769 /label="ORF frame 3" /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRK ERTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAK FSSEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHK ANIEISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTL IFQVELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTS FEAKFLSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI*" promoter 5331..5360 /label="lac_promoter" ORIGIN 1 ATCGATGCAT AATGTGCCTG TCAAATGGAC GAAGCAGGGA TTCTGCAAAC CCTATGCTAC 61 TCCGTCAAGC CGTCAATTGT CTGATTCGTT ACCAATTATG ACAACTTGAC GGCTACATCA 121 TTCACTTTTT CTTCACAACC GGCACGGAAC TCGCTCGGGC TGGCCCCGGT GCATTTTTTA 181 AATACCCGCG AGAAATAGAG TTGATCGTCA AAACCAACAT TGCGACCGAC GGTGGCGATA 241 GGCATCCGGG TGGTGCTCAA AAGCAGCTTC GCCTGGCTGA TACGTTGGTC CTCGCGCCAG 301 CTTAAGACGC TAATCCCTAA CTGCTGGCGG AAAAGATGTG ACAGACGCGA CGGCGACAAG 361 CAAACATGCT GTGCGACGCT GGCGATATCA AAATTGCTGT CTGCCAGGTG ATCGCTGATG 421 TACTGACAAG CCTCGCGTAC CCGATTATCC ATCGGTGGAT GGAGCGACTC GTTAATCGCT 481 TCCATGCGCC GCAGTAACAA TTGCTCAAGC AGATTTATCG CCAGCAGCTC CGAATAGCGC 541 CCTTCCCCTT GCCCGGCGTT AATGATTTGC CCAAACAGGT CGCTGAAATG CGGCTGGTGC 601 GCTTCATCCG GGCGAAAGAA CCCCGTATTG GCAAATATTG ACGGCCAGTT AAGCCATTCA 661 TGCCAGTAGG CGCGCGGACG AAAGTAAACC CACTGGTGAT ACCATTCGCG AGCCTCCGGA 721 TGACGACCGT AGTGATGAAT CTCTCCTGGC GGGAACAGCA AAATATCACC CGGTCGGCAA 781 ACAAATTCTC GTCCCTGATT TTTCACCACC CCCTGACCGC GAATGGTGAG ATTGAGAATA 841 TAACCTTTCA TTCCCAGCGG TCGGTCGATA AAAAAATCGA GATAACCGTT GGCCTCAATC 901 GGCGTTAAAC CCGCCACCAG ATGGGCATTA AACGAGTATC CCGGCAGCAG GGGATCATTT 961 TGCGCTTCAG CCATACTTTT CATACTCCCG CCATTCAGAG AAGAAACCAA TTGTCCATAT 1021 TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT TCTCGCTAAC CAAACCGGTA 1081 ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA AAGCCATGAC AAAAACGCGT 1141 AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG ATTATTTGCA CGGCGTCACA 1201 CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG ATCCTACCTG ACGCTTTTTA 1261 TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG CTAGCAGGAG GAATTCACCA 1321 TGGTACCCGG GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTTGGCT GTTTTGGCGG 1381 ATGAGAGAAG ATTTTCAGCC TGATACAGAT TAAATCAGAA CGCAGAAGCG GTCTGATAAA 1441 ACAGAATTTG CCTGGCGGCA GTAGCGCGGT GGTCCCACCT GACCCCATGC CGAACTCAGA 1501 AGTGAAACGC CGTAGCGCCG ATGGTAGTGT GGGGTCTCCC CATGCGAGAG TAGGGAACTG 1561 CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT 1621 GTTTGTCGGT GAACGCTCTC CTGAGTAGGA CAAATCCGCC GGGAGCGGAT TTGAACGTTG 1681 CGAAGCAACG GCCCGGAGGG TGGCGGGCAG GACGCCCGCC ATAAACTGCC AGGCATCAAA 1741 TTAAGCAGAA GGCCATCCTG ACGGATGGCC TTTTTGCGTT TCTACAAACT CTTTTGTTTA 1801 TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTA GCTTGACTGG 1861 GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG 1921 CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC 1981 GAATGGCGCT AACCGTTTTT ATCAGGCTCT GGGAGGCAGA ATAAATGATC ATATCGTCAA 2041 TTATTACCTC CACGGGGAGA GCCTGAGCAA ACTGGCCTCA GGCATTTGAG AAGCACACGG 2101 TCACACTGCT TCCGGTAGTC AATAAACCGG TAAACCAGCA ATAGACATAA GCGGCTATTT 2161 AACGACCCTG CCCTGAACCG ACGACCGGGT CGAATTTGCT TTCGAATTTC TGCCATTCAT 2221 CCGCTTATTA TCACTTATTC AGGCGTAGCA CCAGGCGTTT AAGGGCACCA ATAACTGCCT 2281 TAAAAAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT 2341 CTGCCGACAT GGAAGCCATC ACAGACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC 2401 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC 2461 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA 2521 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA 2581 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT 2641 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC 2701 ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT CAGGCGGGCA 2761 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG 2821 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC 2881 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT 2941 TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT 3001 AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT 3061 TTTCGCCAAA AGTTGGCCCA GGGCTTCCCG GTATCAACAG GGACACCAGG ATTTATTTAT 3121 TCTGCGAAGT GATCTTCCGT CACAGGTATT TATTCGGCGC AAAGTGCGTC GGGTGATGCT 3181 GCCAACTTAC TGATTTAGTG TATGATGGTG TTTTTGAGGT GCTCCAGTGG CTTCTGTTTC 3241 TATCAGCTGT CCCTCCTGTT CAGCTACTGA CGGGGTGGTG CGTAACGGCA AAAGCACCGC 3301 CGGACATCAG CGCCTGTAGT GCCATTTACC CCCATTCACT GCCAGAGCCG TGAGCGCAGC 3361 GAACTGAATG TCACGAAAAA GACAGCGACT CAGGTGCCTG ATGGTCGGAG ACAAAAGGAA 3421 TATTCAGCGA TTTGCCCGAG CTTGCGAGGG TGCTACTTAA GCCTTTAGGG TTTTAAGGTC 3481 TGTTTTGTAG AGGAGCAAAC AGCGTTTGCG ACATCCTTTT GTAATACTGC GGAACTGACT 3541 AAAGTAGTGA GTTATACACA GGGCTGGGAT CTATTCTTTT TATCTTTTTT TATTCTTTCT 3601 TTATTCTATA AATTATAACC ACTTGAATAT AAACAAAAAA AACACACAAA GGTCTAGCGG 3661 AATTTACAGA GGGTCTAGCA GAATTTACAA GTTTTCCAGC AAAGGTCTAG CAGAATTTAC 3721 AGATACCCAC AACTCAAAGG AAAAGGACTA GTAATTATCA TTGACTAGCC CATCTCAATT 3781 GGTATAGTGA TTAAAATCAC CTAGACCAAT TGAGATGTAT GTCTGAATTA GTTGTTTTCA 3841 AAGCAAATGA ACTAGCGATT AGTCGCTATG ACTTAACGGA GCATGAAACC AAGCTAATTT 3901 TATGCTGTGT GGCACTACTC AACCCCACGA TTGAAAACCC TACAAGGAAA GAACGGACGG 3961 TATCGTTCAC TTATAACCAA TACGCTCAGA TGATGAACAT CAGTAGGGAA AATGCTTATG 4021 GTGTATTAGC TAAAGCAACC AGAGAGCTGA TGACGAGAAC TGTGGAAATC AGGAATCCTT 4081 TGGTTAAAGG CTTTGAGATT TTCCAGTGGA CAAACTATGC CAAGTTCTCA AGCGAAAAAT 4141 TAGAATTAGT TTTTAGTGAA GAGATATTGC CTTATCTTTT CCAGTTAAAA AAATTCATAA 4201 AATATAATCT GGAACATGTT AAGTCTTTTG AAAACAAATA CTCTATGAGG ATTTATGAGT 4261 GGTTATTAAA AGAACTAACA CAAAAGAAAA CTCACAAGGC AAATATAGAG ATTAGCCTTG 4321 ATGAATTTAA GTTCATGTTA ATGCTTGAAA ATAACTACCA TGAGTTTAAA AGGCTTAACC 4381 AATGGGTTTT GAAACCAATA AGTAAAGATT TAAACACTTA CAGCAATATG AAATTGGTGG 4441 TTGATAAGCG AGGCCGCCCG ACTGATACGT TGATTTTCCA AGTTGAACTA GATAGACAAA 4501 TGGATCTCGT AACCGAACTT GAGAACAACC AGATAAAAAT GAATGGTGAC AAAATACCAA 4561 CAACCATTAC ATCAGATTCC TACCTACGTA ACGGACTAAG AAAAACACTA CACGATGCTT 4621 TAACTGCAAA AATTCAGCTC ACCAGTTTTG AGGCAAAATT TTTGAGTGAC ATGCAAAGTA 4681 AGCATGATCT CAATGGTTCG TTCTCATGGC TCACGCAAAA ACAACGAACC ACACTAGAGA 4741 ACATACTGGC TAAATACGGA AGGATCTGAG GTTCTTATGG CTCTTGTATC TATCAGTGAA 4801 GCATCAAGAC TAACAAACAA AAGTAGAACA ACTGTTCACC GTTAGATATC AAAGGGAAAA 4861 CTGTCCATAT GCACAGATGA AAACGGTGTA AAAAAGATAG ATACATCAGA GCTTTTACGA 4921 GTTTTTGGTG CATTTAAAGC TGTTCACCAT GAACAGATCG ACAATGTAAC AGATGAACAG 4981 CATGTAACAC CTAATAGAAC AGGTGAAACC AGTAAAACAA AGCAACTAGA ACATGAAATT 5041 GAACACCTGA GACAACTTGT TACAGCTCAA CAGTCACACA TAGACAGCCT GAAACAGGCG 5101 ATGCTGCTTA TCGAATCAAA GCTGCCGACA ACACGGGAGC CAGTGACGCC TCCCGTGGGG 5161 AAAAAATCAT GGCAATTCTG GAAGAAATAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 5221 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT 5281 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT 5341 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCCTGA TATAGGCGCC AGCAACCGCA 5401 CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCTG CTCATGTTTG 5461 ACAGCTTATC //^pBAD45 "Times New RomanS}C^`AraC"Times New RomanS}C^`ORF 3"Times New RomanS}CɆ^araO2"Times New RomanS}^araO1"Times New RomanS}C^CAP_BS"Times New RomanS}C^ pBAD_fwd_pri"Times New RomanS}CɆ^AraI1I2"Times New Roman*S}^ARA_pro"Times New RomanS}C^w pBAD_rev_pri"Times New RomanS}C^wpTrcHis_rev_pri"Times New RomanS}CɆ^F rrnB_term"Times New RomanS}^$O rrnB_T1_term"Times New RomanS}C^ rrnB_T2_term"Times New RomanS}C^3AmpR_pro"Times New RomanS}CɆ^ ORF 3"Times New RomanS}^ CAT/CamR"Times New RomanS}C^ORF 3"Times New RomanS}C^lac_pro"Times New Roman S}ClaI"Times New Roman^S?++ S}NruI"Times New Roman^<JX}Xn} S}[NheI(1300),EcoRI(1311),KpnI(1326),SmaI(1328),XbaI(1337),SalI(1343),PstI(1353),HindIII(1361)"Times New Roman^|$##_ S}FspI(1966),BclI(2026)"Times New Roman^IYhh S}BstBI"Times New Roman^)44x" S}MscI"Times New Roman ^&99 H S}SpeI"Times New Roman^a S}NdeI"Times New Roman^yiZi S}test"Times New Roman^|Ev?`;`~