Sequence of pBAD_His

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Sequence of pBAD_His_A

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Sequence of pBAD/His A : 4104 BP; 1034 A; 1045 C; 1031 G; 994 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... AgeI(74) .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... NcoI(317) .......... .......... .......... ..........

301 ctaacaggag gaattaacca tggggggttc tcatcatcat catcatcatg gtatggctag 360

301 gattgtcctc cttaattggt accccccaag agtagtagta gtagtagtac cataccgatc 360

.......... .......... .... 6xHis Xpress_fwd_pri T7_gene10_leader T7_leader

.......... .......... .......... .......... .......... ..........

361 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata aggatcgatg 420

361 gtactgacca cctgtcgttt acccagccct agacatgctg ctactgctat tcctagctac 420

Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK ..........

XhoI,SacI,BglII PstI,PvuII KpnI EcoRI,BstBI,HindIII

421 gggatccgag ctcgagatct gcagctggta ccatatggga attcgaagct tggctgtttt 480

421 ccctaggctc gagctctaga cgtcgaccat ggtataccct taagcttcga accgacaaaa 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg 540

481 ccgcctactc tcttctaaaa gtcggactat gtctaattta gtcttgcgtc ttcgccagac 540

.......... .......... . pBAD_rev_pri pTrcHis_rev_pri . ..........

.......... .......... .......... .......... .......... ..........

541 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac 600

541 tattttgtct taaacggacc gccgtcatcg cgccaccagg gtggactggg gtacggcttg 600

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg 660

601 agtcttcact ttgcggcatc gcggctacca tcacacccca gaggggtacg ctctcatccc 660

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 720

661 ttgacggtcc gtagtttatt ttgctttccg agtcagcttt ctgacccgga aagcaaaata 720

.......... ......... rrnB_term rrnB_T1_term ......... ..........

.......... .......... .......... .......... .......... ..........

721 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa 780

721 gacaacaaac agccacttgc gagaggactc atcctgttta ggcggccctc gcctaaactt 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca 840

781 gcaacgcttc gttgccgggc ctcccaccgc ccgtcctgcg ggcggtattt gacggtccgt 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt 900

841 agtttaattc gtcttccggt aggactgcct accggaaaaa cgcaaagatg tttgagaaaa 900

.......... ......... rrnB_T2_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 960

901 acaaataaaa agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt 960

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

961 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 1020

961 tacgaagtta ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa 1020

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

1021 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 1080

1021 taagggaaaa aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt 1080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 1140

1081 cattttctac gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg 1140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 1200

1141 tcgccattct aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa 1200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt 1260

1201 tttcaagacg atacaccgcg ccataatagg gcacaactgc ggcccgttct cgttgagcca 1260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 1320

1261 gcggcgtatg tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta 1320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 1380

1321 gaatgcctac cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg 1380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 1440

1381 tgacgccggt tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac 1440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 1500

1441 gtgttgtacc ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg 1500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 1560

1501 tatggtttgc tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt 1560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... AseI(1601) ..........

1561 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 1620

1561 gataattgac cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 1680

1621 cgcctatttc aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 1740

1681 ctatttagac ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta 1740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 1800

1741 ccattcggga gggcatagca tcaatagatg tgctgcccct cagtccgttg atacctactt 1800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 1860

1801 gctttatctg tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg 1860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 1920

1861 gttcaaatga gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 1980

1921 atccacttct aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2040

1981 gtgactcgca gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac 2040

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2041 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2100

2041 gcgcattaga cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2160

2101 ctagttctcg atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 2220

2161 ttatgacagg aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 2280

2221 ggatgtatgg agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 2340

2281 acagaatggc ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 2400

2341 tgccccccaa gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 2460

2401 gatgtcgcac tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 2520

2461 ggccattcgc cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 2580

2521 accatagaaa tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 2640

2581 acgagcagtc cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 2700

2641 gaccggaaaa cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag 2760

2701 ctattggcat aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg 2820

2761 gcgtcgctca gtcactcgct ccttcgcctt ctcgcggact acgccataaa agaggaatgc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 2880

2821 gtagacacgc cataaagtgt ggcgtatacc acgtgagagt catgttagac gagactacgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 2940

2881 cgtatcaatt cggtcatatg tgaggcgata gcgatgcact gacccagtac cgacgcgggg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 3000

2941 ctgtgggcgg ttgtgggcga ctgcgcggga ctgcccgaac agacgagggc cgtaggcgaa 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 3060

3001 tgtctgttcg acactggcag aggccctcga cgtacacagt ctccaaaagt ggcagtagtg 3060

.......... .......... .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg 3120

3061 gctttgcgcg ctccgtcgtc tagttaagcg cgcgcttccg cttcgccgta cgtattacac 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cctgtcaaat ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa 3180

3121 ggacagttta cctgcttcgt ccctaagacg tttgggatac gatgaggcag ttcggcagtt 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttgtctgatt cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac 3240

3181 aacagactaa gcaatggtta atactgttga actgccgatg tagtaagtga aaaagaagtg 3240

.......... .......... .......... AraC .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aaccggcacg gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat 3300

3241 ttggccgtgc cttgagcgag cccgaccggg gccacgtaaa aaatttatgg gcgctcttta 3300

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agagttgatc gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc 3360

3301 tctcaactag cagttttggt tgtaacgctg gctgccaccg ctatccgtag gcccaccacg 3360

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcaaaagcag cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc 3420

3361 agttttcgtc gaagcggacc gactatgcaa ccaggagcgc ggtcgaattc tgcgattagg 3420

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctaactgctg gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga 3480

3421 gattgacgac cgccttttct acactgtctg cgctgccgct gttcgtttgt acgacacgct 3480

.......... .......... AraC .......... .......... ..........

EcoRV(3488) ........ .......... .......... .......... ..........

3481 cgctggcgat atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc 3540

3481 gcgaccgcta tagttttaac gacagacggt ccactagcga ctacatgact gttcggagcg 3540

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gtacccgatt atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta 3600

3541 catgggctaa taggtagcca cctacctcgc tgagcaatta gcgaaggtac gcggcgtcat 3600

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acaattgctc aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg 3660

3601 tgttaacgag ttcgtctaaa tagcggtcgt cgaggcttat cgcgggaagg ggaacgggcc 3660

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgttaatgat ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa 3720

3661 gcaattacta aacgggtttg tccagcgact ttacgccgac cacgcgaagt aggcccgctt 3720

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agaaccccgt attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg 3780

3721 tcttggggca taaccgttta taactgccgg tcaattcggt aagtacggtc atccgcgcgc 3780

.......... .......... AraC .......... .......... ..........

.......... .......... NruI(3810) .......... .......... ..........

3781 gacgaaagta aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat 3840

3781 ctgctttcat ttgggtgacc actatggtaa gcgctcggag gcctactgct ggcatcacta 3840

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gaatctctcc tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct 3900

3841 cttagagagg accgcccttg tcgttttata gtgggccagc cgtttgttta agagcaggga 3900

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gatttttcac caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca 3960

3901 ctaaaaagtg gtgggggact ggcgcttacc actctaactc ttatattgga aagtaagggt 3960

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcggtcggtc gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca 4020

3961 cgccagccag ctattttttt agctctattg gcaaccggag ttagccgcaa tttgggcggt 4020

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccagatgggc attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac 4080

4021 ggtctacccg taatttgctc atagggccgt cgtcccctag taaaacgcga agtcggtatg 4080

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttttcatact cccgccattc agag...... .......... .......... .......... 4104

4081 aaaagtatga gggcggtaag tctc...... .......... .......... .......... 4104

.......... .......... .......... .......... .......... ..........