Sequence of pBAD_His

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Sequence of pBAD_His_B

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Sequence of pBAD/His B : 4092 BP; 1032 A; 1044 C; 1026 G; 990 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... AgeI(74) .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaacccg 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... NcoI(317) .......... .......... .......... ..........

301 taacaggagg aattaaccat ggggggttct catcatcatc atcatcatgg tatggctagc 360

301 attgtcctcc ttaattggta ccccccaaga gtagtagtag tagtagtacc ataccgatcg 360

.......... .......... .... 6xHis Xpress_fwd_pri T7_gene10_leader T7_leader

.......... .......... .......... .......... .......... ..........

361 atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa ggatccgagc 420

361 tactgaccac ctgtcgttta cccagcccta gacatgctgc tactgctatt cctaggctcg 420

Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK ..........

SacI,BglII PstI,PvuII KpnI EcoRI,BstBI,HindIII55)

421 tcgagatctg cagctggtac catatgggaa ttcgaagctt ggctgttttg gcggatgaga 480

421 agctctagac gtcgaccatg gtataccctt aagcttcgaa ccgacaaaac cgcctactct 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga taaaacagaa 540

481 cttctaaaag tcggactatg tctaatttag tcttgcgtct tcgccagact attttgtctt 540

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

541 tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact cagaagtgaa 600

541 aaacggaccg ccgtcatcgc gccaccaggg tggactgggg tacggcttga gtcttcactt 600

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

601 acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga actgccaggc 660

601 tgcggcatcg cggctaccat cacaccccag aggggtacgc tctcatccct tgacggtccg 660

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 720

661 tagtttattt tgctttccga gtcagctttc tgacccggaa agcaaaatag acaacaaaca 720

......... rrnB_term rrnB_T1_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac gttgcgaagc 780

721 gccacttgcg agaggactca tcctgtttag gcggccctcg cctaaacttg caacgcttcg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat caaattaagc 840

781 ttgccgggcc tcccaccgcc cgtcctgcgg gcggtatttg acggtccgta gtttaattcg 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg tttatttttc 900

841 tcttccggta ggactgccta ccggaaaaac gcaaagatgt ttgagaaaac aaataaaaag 900

......... rrnB_T2_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 960

901 atttatgtaa gtttatacat aggcgagtac tctgttattg ggactattta cgaagttatt 960

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 1020

961 ataacttttt ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa 1020

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

1021 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 1080

1021 cgccgtaaaa cggaaggaca aaaacgagtg ggtctttgcg accactttca ttttctacga 1080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 1140

1081 cttctagtca acccacgtgc tcacccaatg tagcttgacc tagagttgtc gccattctag 1140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 1200

1141 gaactctcaa aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat 1200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac 1260

1201 acaccgcgcc ataatagggc acaactgcgg cccgttctcg ttgagccagc ggcgtatgtg 1260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 1320

1261 ataagagtct tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg 1320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 1380

1321 tactgtcatt ctcttaatac gtcacgacgg tattggtact cactattgtg acgccggttg 1380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 1440

1381 aatgaagact gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc 1440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 1500

1441 ctagtacatt gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg 1500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 1560

1501 ctcgcactgt ggtgctacgg acatcgttac cgttgttgca acgcgtttga taattgaccg 1560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... AseI(1591) .......... ..........

1561 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 1620

1561 cttgatgaat gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa 1620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 1680

1621 cgtcctggtg aagacgcgag ccgggaaggc cgaccgacca aataacgact atttagacct 1680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 1740

1681 cggccactcg cacccagagc gccatagtaa cgtcgtgacc ccggtctacc attcgggagg 1740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 1800

1741 gcatagcatc aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc 1800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 1860

1801 tagcgactct atccacggag tgactaattc gtaaccattg acagtctggt tcaaatgagt 1860

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 1920

1861 atatatgaaa tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 1980

1921 gaaaaactat tagagtactg gttttaggga attgcactca aaagcaaggt gactcgcagt 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 2040

1981 ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc gcattagacg 2040

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2041 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 2100

2041 acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt 2160

2101 ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt atgacaggaa 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 2220

2161 gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 2280

2221 cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac agaatggccc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 2340

2281 aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg ccccccaagc 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 2400

2341 acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 2460

2401 gatactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg ccattcgccg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 2520

2461 tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 2580

2521 tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac gagcagtccc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 2640

2581 cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg 2640

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 2700

2641 accggaaaac gagtgtacaa gaaaggacgc aataggggac taagacacct attggcataa 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 2760

2701 tggcggaaac tcactcgact atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt 2820

2761 cactcgctcc ttcgccttct cgcggactac gccataaaag aggaatgcgt agacacgcca 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc 2880

2821 taaagtgtgg cgtataccac gtgagagtca tgttagacga gactacggcg tatcaattcg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa 2940

2881 gtcatatgtg aggcgatagc gatgcactga cccagtaccg acgcggggct gtgggcggtt 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 3000

2941 gtgggcgact gcgcgggact gcccgaacag acgagggccg taggcgaatg tctgttcgac 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 3060

3001 actggcagag gccctcgacg tacacagtct ccaaaagtgg cagtagtggc tttgcgcgct 3060

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc tgtcaaatgg 3120

3061 ccgtcgtcta gttaagcgcg cgcttccgct tcgccgtacg tattacacgg acagtttacc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg 3180

3121 tgcttcgtcc ctaagacgtt tgggatacga tgaggcagtt cggcagttaa cagactaagc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga 3240

3181 aatggttaat actgttgaac tgccgatgta gtaagtgaaa aagaagtgtt ggccgtgcct 3240

.......... .......... .......... AraC .......... ..........

.......... .......... .......... .......... .......... ..........

3241 actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt 3300

3241 tgagcgagcc cgaccggggc cacgtaaaaa atttatgggc gctctttatc tcaactagca 3300

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct 3360

3301 gttttggttg taacgctggc tgccaccgct atccgtaggc ccaccacgag ttttcgtcga 3360

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc 3420

3361 agcggaccga ctatgcaacc aggagcgcgg tcgaattctg cgattaggga ttgacgaccg 3420

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... ......... EcoRV(3478)

3421 ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat 3480

3421 ccttttctac actgtctgcg ctgccgctgt tcgtttgtac gacacgctgc gaccgctata 3480

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat 3540

3481 gttttaacga cagacggtcc actagcgact acatgactgt tcggagcgca tgggctaata 3540

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa 3600

3541 ggtagccacc tacctcgctg agcaattagc gaaggtacgc ggcgtcattg ttaacgagtt 3600

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt 3660

3601 cgtctaaata gcggtcgtcg aggcttatcg cgggaagggg aacgggccgc aattactaaa 3660

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat 3720

3661 cgggtttgtc cagcgacttt acgccgacca cgcgaagtag gcccgctttc ttggggcata 3720

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa 3780

3721 accgtttata actgccggtc aattcggtaa gtacggtcat ccgcgcgcct gctttcattt 3780

.......... .......... AraC .......... .......... ..........

.......... NruI(3800) .......... .......... .......... ..........

3781 cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg 3840

3781 gggtgaccac tatggtaagc gctcggaggc ctactgctgg catcactact tagagaggac 3840

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca 3900

3841 cgcccttgtc gttttatagt gggccagccg tttgtttaag agcagggact aaaaagtggt 3900

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga 3960

3901 gggggactgg cgcttaccac tctaactctt atattggaaa gtaagggtcg ccagccagct 3960

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat 4020

3961 atttttttag ctctattggc aaccggagtt agccgcaatt tgggcggtgg tctacccgta 4020

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc 4080

4021 atttgctcat agggccgtcg tcccctagta aaacgcgaag tcggtatgaa aagtatgagg 4080

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgccattcag ag........ .......... .......... .......... .......... 4092

4081 gcggtaagtc tc........ .......... .......... .......... .......... 4092

.......... .......... .......... .......... .......... ..........