Sequence of pBAD_His

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Sequence of pBAD_His_C

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Sequence of pBAD/His C : 4102 BP; 1035 A; 1046 C; 1027 G; 994 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... AgeI(74) .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... NcoI(317) .......... .......... .......... ..........

301 ctaacaggag gaattaacca tggggggttc tcatcatcat catcatcatg gtatggctag 360

301 gattgtcctc cttaattggt accccccaag agtagtagta gtagtagtac cataccgatc 360

.......... .......... .... 6xHis Xpress_fwd_pri T7_gene10_leader T7_leader

.......... .......... .......... .......... .......... ..........

361 catgactggt ggacagcaaa tgggtcggga tctgtacgac gatgacgata agcatcgatg 420

361 gtactgacca cctgtcgttt acccagccct agacatgctg ctactgctat tcgtagctac 420

Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK ..........

XhoI,BglII PstI KpnI EcoRI,BstBI460),HindIII(463)

421 gatccgacct cgagatctgc agatggtacc atatgggaat tcgaagcttg gctgttttgg 480

421 ctaggctgga gctctagacg tctaccatgg tataccctta agcttcgaac cgacaaaacc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat 540

481 gcctactctc ttctaaaagt cggactatgt ctaatttagt cttgcgtctt cgccagacta 540

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc 600

541 ttttgtctta aacggaccgc cgtcatcgcg ccaccagggt ggactggggt acggcttgag 600

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

601 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa 660

601 tcttcacttt gcggcatcgc ggctaccatc acaccccaga ggggtacgct ctcatccctt 660

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 720

661 gacggtccgt agtttatttt gctttccgag tcagctttct gacccggaaa gcaaaataga 720

.......... ......... rrnB_term rrnB_T1_term ......... ..........

.......... .......... .......... .......... .......... ..........

721 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg 780

721 caacaaacag ccacttgcga gaggactcat cctgtttagg cggccctcgc ctaaacttgc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc 840

781 aacgcttcgt tgccgggcct cccaccgccc gtcctgcggg cggtatttga cggtccgtag 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actctttttg 900

841 tttaattcgt cttccggtag gactgcctac cggaaaaacg caaagatgtt tgagaaaaac 900

.......... ......... rrnB_T2_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 960

901 aaataaaaag atttatgtaa gtttatacat aggcgagtac tctgttattg ggactattta 960

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 1020

961 cgaagttatt ataacttttt ccttctcata ctcataagtt gtaaaggcac agcgggaata 1020

.......... .......... .......... ....... ORF 3 Ampicillin .......

.......... .......... .......... .......... .......... ..........

1021 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 1080

1021 agggaaaaaa cgccgtaaaa cggaaggaca aaaacgagtg ggtctttgcg accactttca 1080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 1140

1081 ttttctacga cttctagtca acccacgtgc tcacccaatg tagcttgacc tagagttgtc 1140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 1200

1141 gccattctag gaactctcaa aagcggggct tcttgcaaaa ggttactact cgtgaaaatt 1200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 1260

1201 tcaagacgat acaccgcgcc ataatagggc acaactgcgg cccgttctcg ttgagccagc 1260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 1320

1261 ggcgtatgtg ataagagtct tactgaacca actcatgagt ggtcagtgtc ttttcgtaga 1320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 1380

1321 atgcctaccg tactgtcatt ctcttaatac gtcacgacgg tattggtact cactattgtg 1380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 1440

1381 acgccggttg aatgaagact gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt 1440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1500

1441 gttgtacccc ctagtacatt gagcggaact agcaaccctt ggcctcgact tacttcggta 1500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1560

1501 tggtttgctg ctcgcactgt ggtgctacgg acatcgttac cgttgttgca acgcgtttga 1560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... AseI(1599) .......... ..........

1561 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1620

1561 taattgaccg cttgatgaat gagatcgaag ggccgttgtt aattatctga cctacctccg 1620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1680

1621 cctatttcaa cgtcctggtg aagacgcgag ccgggaaggc cgaccgacca aataacgact 1680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1740

1681 atttagacct cggccactcg cacccagagc gccatagtaa cgtcgtgacc ccggtctacc 1740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1800

1741 attcgggagg gcatagcatc aatagatgtg ctgcccctca gtccgttgat acctacttgc 1800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1860

1801 tttatctgtc tagcgactct atccacggag tgactaattc gtaaccattg acagtctggt 1860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1920

1861 tcaaatgagt atatatgaaa tctaactaaa ttttgaagta aaaattaaat tttcctagat 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1980

1921 ccacttctag gaaaaactat tagagtactg gttttaggga attgcactca aaagcaaggt 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 2040

1981 gactcgcagt ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc 2040

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2041 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 2100

2041 gcattagacg acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 2160

2101 agttctcgat ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 2220

2161 atgacaggaa gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 2280

2221 atgtatggag cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 2340

2281 agaatggccc aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 2400

2341 ccccccaagc acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 2460

2401 tgtcgcactc gatactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2520

2461 ccattcgccg tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2580

2521 catagaaata tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2640

2581 gagcagtccc cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2700

2641 ccggaaaacg accggaaaac gagtgtacaa gaaaggacgc aataggggac taagacacct 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2760

2701 attggcataa tggcggaaac tcactcgact atggcgagcg gcgtcggctt gctggctcgc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2820

2761 gtcgctcagt cactcgctcc ttcgccttct cgcggactac gccataaaag aggaatgcgt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2880

2821 agacacgcca taaagtgtgg cgtataccac gtgagagtca tgttagacga gactacggcg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2940

2881 tatcaattcg gtcatatgtg aggcgatagc gatgcactga cccagtaccg acgcggggct 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 3000

2941 gtgggcggtt gtgggcgact gcgcgggact gcccgaacag acgagggccg taggcgaatg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 3060

3001 tctgttcgac actggcagag gccctcgacg tacacagtct ccaaaagtgg cagtagtggc 3060

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 3120

3061 tttgcgcgct ccgtcgtcta gttaagcgcg cgcttccgct tcgccgtacg tattacacgg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 3180

3121 acagtttacc tgcttcgtcc ctaagacgtt tgggatacga tgaggcagtt cggcagttaa 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 3240

3181 cagactaagc aatggttaat actgttgaac tgccgatgta gtaagtgaaa aagaagtgtt 3240

.......... .......... .......... AraC .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 3300

3241 ggccgtgcct tgagcgagcc cgaccggggc cacgtaaaaa atttatgggc gctctttatc 3300

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 3360

3301 tcaactagca gttttggttg taacgctggc tgccaccgct atccgtaggc ccaccacgag 3360

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 3420

3361 ttttcgtcga agcggaccga ctatgcaacc aggagcgcgg tcgaattctg cgattaggga 3420

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3480

3421 ttgacgaccg ccttttctac actgtctgcg ctgccgctgt tcgtttgtac gacacgctgc 3480

.......... .......... AraC .......... .......... ..........

EcoRV(3486) ........ .......... .......... .......... ..........

3481 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3540

3481 gaccgctata gttttaacga cagacggtcc actagcgact acatgactgt tcggagcgca 3540

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3600

3541 tgggctaata ggtagccacc tacctcgctg agcaattagc gaaggtacgc ggcgtcattg 3600

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3660

3601 ttaacgagtt cgtctaaata gcggtcgtcg aggcttatcg cgggaagggg aacgggccgc 3660

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3720

3661 aattactaaa cgggtttgtc cagcgacttt acgccgacca cgcgaagtag gcccgctttc 3720

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3780

3721 ttggggcata accgtttata actgccggtc aattcggtaa gtacggtcat ccgcgcgcct 3780

.......... .......... AraC .......... .......... ..........

.......... .......... NruI(3808) .......... .......... ..........

3781 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3840

3781 gctttcattt gggtgaccac tatggtaagc gctcggaggc ctactgctgg catcactact 3840

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3900

3841 tagagaggac cgcccttgtc gttttatagt gggccagccg tttgtttaag agcagggact 3900

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3960

3901 aaaaagtggt gggggactgg cgcttaccac tctaactctt atattggaaa gtaagggtcg 3960

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 4020

3961 ccagccagct atttttttag ctctattggc aaccggagtt agccgcaatt tgggcggtgg 4020

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 4080

4021 tctacccgta atttgctcat agggccgtcg tcccctagta aaacgcgaag tcggtatgaa 4080

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttcatactcc cgccattcag ag........ .......... .......... .......... 4102

4081 aagtatgagg gcggtaagtc tc........ .......... .......... .......... 4102

.......... .......... .......... .......... .......... ..........