Sequence of pBAD_His

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Sequence of pBAD_His_LacZ

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Sequence of pBAD/His/LacZ : 7241 BP; 1730 A; 1915 C; 1924 G; 1672 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... AgeI(74) .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaacccg 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... NcoI(317) SpeI(327) .......... .......... ..........

301 taacaggagg aattaaccat ggatccacta gtaacggccg ccagtgtgct ggaattcggc 360

301 attgtcctcc ttaattggta cctaggtgat cattgccggc ggtcacacga ccttaagccg 360

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

361 ttgttcgaaa cgatgataga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc 420

361 aacaagcttt gctactatct agggcagcaa aatgttgcag cactgaccct tttgggaccg 420

.......... ....... ORF 1 M13_pUC_fwd_pri lacZ .......

.......... .......... .......... .......... .......... ..........

421 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 480

421 caatgggttg aattagcgga acgtcgtgta gggggaaagc ggtcgaccgc attatcgctt 480

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt 540

481 ctccgggcgt ggctagcggg aagggttgtc aacgcgtcgg acttaccgct taccgcgaaa 540

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gcctggtttc cggtaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag 600

541 cggaccaaag gccatggtct tcgccacggc ctttcgaccg acctcacgct agaaggactc 600

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac 660

601 cggctatgac agcagcaggg gagtttgacc gtctacgtgc caatgctacg cgggtagatg 660

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg 720

661 tggttgcatt ggatagggta atgccagtta ggcggcaaac aagggtgcct cttaggctgc 720

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga 780

721 ccaacaatga gcgagtgtaa attacaacta ctttcgaccg atgtccttcc ggtctgcgct 780

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt 840

781 taataaaaac taccgcaatt gagccgcaaa gtagacacca cgttgcccgc gacccagcca 840

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga 900

841 atgccggtcc tgtcagcaaa cggcagactt aaactggact cgcgtaaaaa tgcgcggcct 900

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gaaaaccgcc tcgcggtgat ggtgctgcgt tggagtgacg gcagttatct ggaagatcag 960

901 cttttggcgg agcgccacta ccacgacgca acctcactgc cgtcaataga ccttctagtc 960

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... AatII(995) .......... ..........

961 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca 1020

961 ctatacaccg cctactcgcc gtaaaaggca ctgcagagca acgacgtatt tggctgatgt 1020

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg 1080

1021 gtttagtcgc taaaggtaca acggtgagcg aaattactac taaagtcggc gcgacatgac 1080

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta 1140

1081 ctccgacttc aagtctacac gccgctcaac gcactgatgg atgcccattg tcaaagaaat 1140

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ClaI(1196)

1141 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat 1200

1141 accgtcccac tttgcgtcca gcggtcgccg tggcgcggaa agccgccact ttaatagcta 1200

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg 1260

1201 ctcgcaccac caatacggct agcgcagtgt gatgcagact tgcagctttt gggctttgac 1260

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc 1320

1261 acctcgcggc tttagggctt agagatagca cgccaccaac ttgacgtgtg gcggctgccg 1320

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt 1380

1321 tgcgactaac ttcgtcttcg gacgctacag ccaaaggcgc tccacgccta acttttacca 1380

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat 1440

1381 gacgacgacg acttgccgtt cggcaacgac taagctccgc aattggcagt gctcgtagta 1440

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag 1500

1441 ggagacgtac cagtccagta cctactcgtc tgctaccacg tcctatagga cgactacttc 1500

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg 1560

1501 gtcttgttga aattgcggca cgcgacaagc gtaataggct tggtaggcga caccatgtgc 1560

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg 1620

1561 gacacgctgg cgatgccgga catacaccac ctacttcggt tataactttg ggtgccgtac 1620

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta 1680

1621 cacggttact tagcagactg gctactaggc gcgaccgatg gccgctactc gcttgcgcat 1680

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... BclI(1718) .......... ..........

1681 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat 1740

1681 tgcgcttacc acgtcgcgct agcattagtg ggctcacact agtagaccag cgacccctta 1740

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct 1800

1741 cttagtccgg tgccgcgatt agtgctgcgc gacatagcga cctagtttag acagctagga 1800

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc 1860

1801 agggcgggcc acgtcatact tccgccgcct cggctgtggt gccggtggct ataataaacg 1860

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc 1920

1861 ggctacatgc gcgcgcacct acttctggtc gggaagggcc gacacggctt taccaggtag 1920

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac 1980

1921 ttttttaccg aaagcgatgg acctctctgc gcgggcgact aggaaacgct tatgcgggtg 1980

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc 2040

1981 cgctacccat tgtcagaacc gccaaagcga tttatgaccg tccgcaaagc agtcataggg 2040

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa 2100

2041 gcaaatgtcc cgccgaagca gaccctgacc cacctagtca gcgactaatt tatactactt 2100

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag 2160

2101 ttgccgttgg gcaccagccg aatgccgcca ctaaaaccgc tatgcggctt gctagcggtc 2160

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca 2220

2161 aagacatact tgccagacca gaaacggctg gcgtgcggcg taggtcgcga ctgccttcgt 2220

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc 2280

2221 tttgtggtcg tcgtcaaaaa ggtcaaggca aataggcccg tttggtagct tcactggtcg 2280

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... SacI(2312) .......... ..........

2281 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt 2340

2281 cttatggaca aggcagtatc gctattgctc gaggacgtga cctaccaccg cgacctacca 2340

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt 2400

2341 ttcggcgacc gttcgccact tcacggagac ctacagcgag gtgttccatt tgtcaactaa 2400

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta 2460

2401 cttgacggac ttgatggcgt cggcctctcg cggcccgttg agaccgagtg tcatgcgcat 2460

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg 2520

2461 cacgttggct tgcgctggcg taccagtctt cggcccgtgt agtcgcggac cgtcgtcacc 2520

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg 2580

2521 gcagaccgcc ttttggagtc acactgcgag gggcggcgca gggtgcggta gggcgtagac 2580

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc 2640

2581 tggtggtcgc tttacctaaa aacgtagctc gacccattat tcgcaaccgt taaattggcg 2640

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg 2700

2641 gtcagtccga aagaaagtgt ctacacctaa ccgctatttt ttgttgacga ctgcggcgac 2700

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc 2760

2701 gcgctagtca agtgggcacg tggcgaccta ttgctgtaac cgcattcact tcgctgggcg 2760

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca 2820

2761 taactgggat tgcggaccca gcttgcgacc ttccgccgcc cggtaatggt ccggcttcgt 2820

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac 2880

2821 cgcaacaacg tcacgtgccg tctatgtgaa cgactacgcc acgactaatg ctggcgagtg 2880

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt 2940

2881 cgcaccgtcg tagtcccctt ttggaataaa tagtcggcct tttggatggc ctaactacca 2940

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg 3000

2941 tcaccagttt accgctaatg gcaactacaa cttcaccgct cgctatgtgg cgtaggccgc 3000

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta 3060

3001 gcctaaccgg acttgacggt cgaccgcgtc catcgtctcg cccatttgac cgagcctaat 3060

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg 3120

3061 cccggcgttc ttttgatagg gctggcggaa tgacggcgga caaaactggc gaccctagac 3120

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg 3180

3121 ggtaacagtc tgtacatatg gggcatgcag aagggctcgc ttttgccaga cgcgacgccc 3180

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc 3240

3181 tgcgcgctta acttaatacc gggtgtggtc accgcgccgc tgaaggtcaa gttgtagtcg 3240

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa 3300

3241 gcgatgtcag ttgtcgttga ctacctttgg tcggtagcgg tagacgacgt gcgccttctt 3300

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc 3360

3301 ccgtgtaccg acttatagct gccaaaggta tacccctaac caccgctgct gaggacctcg 3360

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ccgtcagtat cggcggaatt ccagctgagc gccggtcgct accattacca gttggtctgg 3420

3361 ggcagtcata gccgccttaa ggtcgactcg cggccagcga tggtaatggt caaccagacc 3420

.......... .......... ORF 1 lacZ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgtcaaaaag cggccgcaag ccgaattctg cagatatcca tcacactggc ggccgctcga 3480

3421 acagtttttc gccggcgttc ggcttaagac gtctataggt agtgtgaccg ccggcgagct 3480

lacZ .......... ORF 1 .......... .......... ..........

.......... .......... ........ HindIII(3511) ........ ..........

3481 gatctgcagc tggtaccata tgggaattcg aagcttgggc ccgaacaaaa actcatctca 3540

3481 ctagacgtcg accatggtat acccttaagc ttcgaacccg ggcttgtttt tgagtagagt 3540

.......... .......... ORF 1 .......... .......... c_myc

.......... .......... SalI(3562) .......... .......... ..........

3541 gaagaggatc tgaatagcgc cgtcgaccat catcatcatc atcattgagt ttaaacggtc 3600

3541 cttctcctag acttatcgcg gcagctggta gtagtagtag tagtaactca aatttgccag 3600

c_myc .......... ORF 1 6xHis .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tccagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag 3660

3601 aggtcgaacc gacaaaaccg cctactctct tctaaaagtc ggactatgtc taatttagtc 3660

.......... .......... .......... . pBAD_rev_pri pTrcHis_rev_pri .

.......... .......... .......... .......... .......... ..........

3661 aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac 3720

3661 ttgcgtcttc gccagactat tttgtcttaa acggaccgcc gtcatcgcgc caccagggtg 3720

.......... .......... .......... .......... rrnB_term ..........

.......... .......... .......... .......... .......... ..........

3721 ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc 3780

3721 gactggggta cggcttgagt cttcactttg cggcatcgcg gctaccatca caccccagag 3780

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca g tcgaaagac 3840

3781 gggtacgctc tcatcccttg acggtccgta gtttattttg ctttccgagt cagctttctg 3840

.......... .......... rrnB_term ......... rrnB_T1_term .........

.......... .......... .......... .......... .......... ..........

3841 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg 3900

3841 acccggaaag caaaatagac aacaaacagc cacttgcgag aggactcatc ctgtttaggc 3900

rrnB_T1_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg 3960

3901 ggccctcgcc taaacttgca acgcttcgtt gccgggcctc ccaccgcccg tcctgcgggc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg 4020

3961 ggtatttgac ggtccgtagt ttaattcgtc ttccggtagg actgcctacc ggaaaaacgc 4020

.......... .......... .......... ......... rrnB_T2_term .........

.......... .......... .......... .......... .......... ..........

4021 tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 4080

4021 aaagatgttt gagaaaaaca aataaaaaga tttatgtaag tttatacata ggcgagtact 4080

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4081 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 4140

4081 ctgttattgg gactatttac gaagttatta taactttttc cttctcatac tcataagttg 4140

.......... .......... .......... .......... ....... ORF 3 Ampicillin

.......... .......... .......... .......... .......... ..........

4141 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 4200

4141 taaaggcaca gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa aaacgagtgg 4200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 4260

4201 gtctttgcga ccactttcat tttctacgac ttctagtcaa cccacgtgct cacccaatgt 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 4320

4261 agcttgacct agagttgtcg ccattctagg aactctcaaa agcggggctt cttgcaaaag 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 4380

4321 gttactactc gtgaaaattt caagacgata caccgcgcca taatagggca caactgcggc 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 4440

4381 ccgttctcgt tgagccagcg gcgtatgtga taagagtctt actgaaccaa ctcatgagtg 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 4500

4441 gtcagtgtct tttcgtagaa tgcctaccgt actgtcattc tcttaatacg tcacgacggt 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 4560

4501 attggtactc actattgtga cgccggttga atgaagactg ttgctagcct cctggcttcc 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 4620

4561 tcgattggcg aaaaaacgtg ttgtaccccc tagtacattg agcggaacta gcaacccttg 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 4680

4621 gcctcgactt acttcggtat ggtttgctgc tcgcactgtg gtgctacgga catcgttacc 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... AseI(4740)

4681 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 4740

4681 gttgttgcaa cgcgtttgat aattgaccgc ttgatgaatg agatcgaagg gccgttgtta 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 4800

4741 attatctgac ctacctccgc ctatttcaac gtcctggtga agacgcgagc cgggaaggcc 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 4860

4801 gaccgaccaa ataacgacta tttagacctc ggccactcgc acccagagcg ccatagtaac 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 4920

4861 gtcgtgaccc cggtctacca ttcgggaggg catagcatca atagatgtgc tgcccctcag 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 4980

4921 tccgttgata cctacttgct ttatctgtct agcgactcta tccacggagt gactaattcg 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 5040

4981 taaccattga cagtctggtt caaatgagta tatatgaaat ctaactaaat tttgaagtaa 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 5100

5041 aaattaaatt ttcctagatc cacttctagg aaaaactatt agagtactgg ttttagggaa 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 5160

5101 ttgcactcaa aagcaaggtg actcgcagtc tggggcatct tttctagttt cctagaagaa 5160

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

5161 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 5220

5161 ctctaggaaa aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 5280

5221 gccaccaaac aaacggccta gttctcgatg gttgagaaaa aggcttccat tgaccgaagt 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 5340

5281 cgtctcgcgt ctatggttta tgacaggaag atcacatcgg catcaatccg gtggtgaagt 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 5400

5341 tcttgagaca tcgtggcgga tgtatggagc gagacgatta ggacaatggt caccgacgac 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 5460

5401 ggtcaccgct attcagcaca gaatggccca acctgagttc tgctatcaat ggcctattcc 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 5520

5461 gcgtcgccag cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 5580

5521 tgtggcttga ctctatggat gtcgcactcg atactctttc gcggtgcgaa gggcttccct 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 5640

5581 ctttccgcct gtccataggc cattcgccgt cccagccttg tcctctcgcg tgctccctcg 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 5700

5641 aaggtccccc tttgcggacc atagaaatat caggacagcc caaagcggtg gagactgaac 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 5760

5701 tcgcagctaa aaacactacg agcagtcccc ccgcctcgga tacctttttg cggtcgttgc 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 5820

5761 gccggaaaaa tgccaaggac cggaaaacga ccggaaaacg agtgtacaag aaaggacgca 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 5880

5821 ataggggact aagacaccta ttggcataat ggcggaaact cactcgacta tggcgagcgg 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 5940

5881 cgtcggcttg ctggctcgcg tcgctcagtc actcgctcct tcgccttctc gcggactacg 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta 6000

5941 ccataaaaga ggaatgcgta gacacgccat aaagtgtggc gtataccacg tgagagtcat 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 6060

6001 gttagacgag actacggcgt atcaattcgg tcatatgtga ggcgatagcg atgcactgac 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 6120

6061 ccagtaccga cgcggggctg tgggcggttg tgggcgactg cgcgggactg cccgaacaga 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 6180

6121 cgagggccgt aggcgaatgt ctgttcgaca ctggcagagg ccctcgacgt acacagtctc 6180

.......... .......... .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

6181 gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa 6240

6181 caaaagtggc agtagtggct ttgcgcgctc cgtcgtctag ttaagcgcgc gcttccgctt 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta 6300

6241 cgccgtacgt attacacgga cagtttacct gcttcgtccc taagacgttt gggatacgat 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc 6360

6301 gaggcagttc ggcagttaac agactaagca atggttaata ctgttgaact gccgatgtag 6360

.......... .......... .......... .......... AraC ..........

.......... .......... .......... .......... .......... ..........

6361 attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt 6420

6361 taagtgaaaa agaagtgttg gccgtgcctt gagcgagccc gaccggggcc acgtaaaaaa 6420

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat 6480

6421 tttatgggcg ctctttatct caactagcag ttttggttgt aacgctggct gccaccgcta 6480

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca 6540

6481 tccgtaggcc caccacgagt tttcgtcgaa gcggaccgac tatgcaacca ggagcgcggt 6540

.......... .......... AraC .......... .......... ..........

AflII(6542) ........ .......... .......... .......... ..........

6541 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa 6600

6541 cgaattctgc gattagggat tgacgaccgc cttttctaca ctgtctgcgc tgccgctgtt 6600

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat 6660

6601 cgtttgtacg acacgctgcg accgctatag ttttaacgac agacggtcca ctagcgacta 6660

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc 6720

6661 catgactgtt cggagcgcat gggctaatag gtagccacct acctcgctga gcaattagcg 6720

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg 6780

6721 aaggtacgcg gcgtcattgt taacgagttc gtctaaatag cggtcgtcga ggcttatcgc 6780

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg 6840

6781 gggaagggga acgggccgca attactaaac gggtttgtcc agcgacttta cgccgaccac 6840

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt taagccattc 6900

6841 gcgaagtagg cccgctttct tggggcataa ccgtttataa ctgccggtca attcggtaag 6900

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... NruI(6949) ..........

6901 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg 6960

6901 tacggtcatc cgcgcgcctg ctttcatttg ggtgaccact atggtaagcg ctcggaggcc 6960

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca 7020

6961 tactgctggc atcactactt agagaggacc gcccttgtcg ttttatagtg ggccagccgt 7020

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat 7080

7021 ttgtttaaga gcagggacta aaaagtggtg ggggactggc gcttaccact ctaactctta 7080

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat 7140

7081 tattggaaag taagggtcgc cagccagcta tttttttagc tctattggca accggagtta 7140

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt 7200

7141 gccgcaattt gggcggtggt ctacccgtaa tttgctcata gggccgtcgt cccctagtaa 7200

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ttgcgcttca gccatacttt tcatactccc gccattcaga g......... .......... 7241

7201 aacgcgaagt cggtatgaaa agtatgaggg cggtaagtct c......... .......... 7241

AraC .......... .......... .......... .......... ..........