!LOCUS pBAD/Thio-TOPO 4454 bp DNA SYN 26-Oct-2007 DEFINITION pBAD/Thio-TOPO ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4454 /organism="pBAD/Thio-TOPO" /mol_type="other DNA" CDS complement(3550..25) /label="ORF frame 1" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 4..19 /label="araO2" misc_feature 161..172 /label="araO1" misc_feature 203..216 /label="CAP_BS" misc_feature 208..227 /label="pBAD_fwd_primer" misc_feature 213..251 /label="AraI1I2" promoter 248..276 /label="ARA_promoter" misc_feature 317..333 /label="T7_transl_en_RBS" misc_feature 346..674 /label="His_patch_thio" misc_feature 691..705 /label="EK" misc_feature 730..771 /label="V5" misc_feature 781..798 /label="6xHis" misc_feature complement(854..871) /label="pBAD_rev_primer" misc_feature complement(854..871) /label="pTrcHis_rev_primer" terminator 904..1061 /label="rrnB_terminator" terminator 1027..1070 /label="rrnB_T1_terminator" terminator 1202..1229 /label="rrnB_T2_terminator" promoter 1271..1299 /label="AmpR_promoter" CDS 1341..2201 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 1341..2201 /label="Ampicillin" /gene="Ampicillin" rep_origin 2356..2975 /label="pBR322_origin" misc_feature 3372..3394 /label="pGEX_3_primer" misc_feature complement(3550..4428) /label="AraC" ORIGIN 1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT 61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA 121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG 181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG 241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTTGGG 301 CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACA TACCCATGGG ATCTGATAAA 361 ATTATTCATC TGACTGATGA TTCTTTTGAT ACTGATGTAC TTAAGGCAGA TGGTGCAATC 421 CTGGTTGATT TCTGGGCACA CTGGTGCGGT CCGTGCAAAA TGATCGCTCC GATTCTGGAT 481 GAAATCGCTG ACGAATATCA GGGCAAACTG ACCGTTGCAA AACTGAACAT CGATCACAAC 541 CCGGGCACTG CGCCGAAATA TGGCATCCGT GGTATCCCGA CTCTGCTGCT GTTCAAAAAC 601 GGTGAAGTGG CGGCAACCAA AGTGGGTGCA CTGTCTAAAG GTCAGTTGAA AGAGTTCCTC 661 GACGCTAACC TGGCCGGCTC TGGATCCGGT GATGACGATG ACAAGCTCGC CCTTAAGGGC 721 GAGCTTGAAG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GCGTACCGGT 781 CATCATCACC ATCACCATTG AGTTTAAACG GTCTCCAGCT TGGCTGTTTT GGCGGATGAG 841 AGAAGATTTT CAGCCTGATA CAGATTAAAT CAGAACGCAG AAGCGGTCTG ATAAAACAGA 901 ATTTGCCTGG CGGCAGTAGC GCGGTGGTCC CACCTGACCC CATGCCGAAC TCAGAAGTGA 961 AACGCCGTAG CGCCGATGGT AGTGTGGGGT CTCCCCATGC GAGAGTAGGG AACTGCCAGG 1021 CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG 1081 TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG 1141 CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA TCAAATTAAG 1201 CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC AAACTCTTTT TGTTTATTTT 1261 TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT 1321 AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT 1381 TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG 1441 CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA 1501 TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC 1561 TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC 1621 ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG 1681 GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA 1741 ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG 1801 GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG 1861 ACGAGCGTGA CACCACGATG CCTGTAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG 1921 GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG 1981 TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG 2041 GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT 2101 CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC 2161 AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT 2221 CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA 2281 TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT 2341 CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT 2401 GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC 2461 TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC 2521 TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC 2581 TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG 2641 GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT 2701 CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG 2761 AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG 2821 GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT 2881 ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 2941 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 3001 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 3061 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 3121 CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG 3181 GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA 3241 GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC 3301 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC 3361 TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC 3421 GAGGCAGCAG ATCAATTCGC GCGCGAAGGC GAAGCGGCAT GCATAATGTG CCTGTCAAAT 3481 GGACGAAGCA GGGATTCTGC AAACCCTATG CTACTCCGTC AAGCCGTCAA TTGTCTGATT 3541 CGTTACCAAT TATGACAACT TGACGGCTAC ATCATTCACT TTTTCTTCAC AACCGGCACG 3601 GAACTCGCTC GGGCTGGCCC CGGTGCATTT TTTAAATACC CGCGAGAAAT AGAGTTGATC 3661 GTCAAAACCA ACATTGCGAC CGACGGTGGC GATAGGCATC CGGGTGGTGC TCAAAAGCAG 3721 CTTCGCCTGG CTGATACGTT GGTCCTCGCG CCAGCTTAAG ACGCTAATCC CTAACTGCTG 3781 GCGGAAAAGA TGTGACAGAC GCGACGGCGA CAAGCAAACA TGCTGTGCGA CGCTGGCGAT 3841 ATCAAAATTG CTGTCTGCCA GGTGATCGCT GATGTACTGA CAAGCCTCGC GTACCCGATT 3901 ATCCATCGGT GGATGGAGCG ACTCGTTAAT CGCTTCCATG CGCCGCAGTA ACAATTGCTC 3961 AAGCAGATTT ATCGCCAGCA GCTCCGAATA GCGCCCTTCC CCTTGCCCGG CGTTAATGAT 4021 TTGCCCAAAC AGGTCGCTGA AATGCGGCTG GTGCGCTTCA TCCGGGCGAA AGAACCCCGT 4081 ATTGGCAAAT ATTGACGGCC AGTTAAGCCA TTCATGCCAG TAGGCGCGCG GACGAAAGTA 4141 AACCCACTGG TGATACCATT CGCGAGCCTC CGGATGACGA CCGTAGTGAT GAATCTCTCC 4201 TGGCGGGAAC AGCAAAATAT CACCCGGTCG GCAAACAAAT TCTCGTCCCT GATTTTTCAC 4261 CACCCCCTGA CCGCGAATGG TGAGATTGAG AATATAACCT TTCATTCCCA GCGGTCGGTC 4321 GATAAAAAAA TCGAGATAAC CGTTGGCCTC AATCGGCGTT AAACCCGCCA CCAGATGGGC 4381 ATTAAACGAG TATCCCGGCA GCAGGGGATC ATTTTGCGCT TCAGCCATAC TTTTCATACT 4441 CCCGCCATTC AGAG // 0 pBAD/Thio "Times New Roman!C0 ORF 1"Times New Roman(CɆ0 AraC"Times New RomanC0araO2"Times New RomanCɆ0araO1"Times New Roman0CAP_BS"Times New RomanC0 pBAD_fwd_pri"Times New RomanC0AraI1I2"Times New Roman*CɆ0ARA_pro"Times New Roman0=MT7_transl_en_RBS"Times New RomanC0ZHis_patch_thio"Times New RomanC0EK"Times New RomanCɆ0V5"Times New Roman0 6xHis"Times New RomanC0DU pBAD_rev_pri"Times New RomanC0DUpTrcHis_rev_pri"Times New RomanCɆ0v rrnB_term"Times New Roman0 rrnB_T1_term"Times New RomanC0 rrnB_T2_term"Times New RomanC0AmpR_pro"Times New RomanCɆ0+ORF 3"Times New Roman0+ Ampicillin"Times New RomanC0"  pBR322_origin"Times New RomanC0 0  pGEX_3_pri"Times New Roman NcoI"Times New RomanX0#@ ClaI(530),SmaI(542)"Times New Roman0AO\\ HindIII"Times New Roman0e v   FspI(1887),AseI(1935)"Times New Roman_#0 l~##f NdeI"Times New Romani 0[ EcoRV"Times New Roman0 NruI"Times New Roman00RKC$b test"Times New Romanc0}O^U$S$ test"Times New Roman0E& test"Times New Roman0yII<