Sequence of pBAD

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Sequence of pBAD_Thio

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Sequence of pBAD/Thio : 4454 BP; 1124 A; 1138 C; 1103 G; 1089 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaaaccc 300

AraI1I2 .......... ARA_pro .......... .......... ..........

.......... .......... .......... .......... NcoI(344) ..........

301 ctagaaataa ttttgtttaa ctttaagaag gagatataca tacccatggg atctgataaa 360

301 gatctttatt aaaacaaatt gaaattcttc ctctatatgt atgggtaccc tagactattt 360

.......... ....... T7_transl_en_RBS ....... ........ His_patch_thio

.......... .......... .......... .......... .......... ..........

361 attattcatc tgactgatga ttcttttgat actgatgtac ttaaggcaga tggtgcaatc 420

361 taataagtag actgactact aagaaaacta tgactacatg aattccgtct accacgttag 420

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctggttgatt tctgggcaca ctggtgcggt ccgtgcaaaa tgatcgctcc gattctggat 480

421 gaccaactaa agacccgtgt gaccacgcca ggcacgtttt actagcgagg ctaagaccta 480

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 gaaatcgctg acgaatatca gggcaaactg accgttgcaa aactgaacat cgatcacaac 540

481 ctttagcgac tgcttatagt cccgtttgac tggcaacgtt ttgacttgta gctagtgttg 540

.......... ........ His_patch_thio ........ .......... ..........

SmaI(542) .......... .......... .......... .......... ..........

541 ccgggcactg cgccgaaata tggcatccgt ggtatcccga ctctgctgct gttcaaaaac 600

541 ggcccgtgac gcggctttat accgtaggca ccatagggct gagacgacga caagtttttg 600

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggtgaagtgg cggcaaccaa agtgggtgca ctgtctaaag gtcagttgaa agagttcctc 660

601 ccacttcacc gccgttggtt tcacccacgt gacagatttc cagtcaactt tctcaaggag 660

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... ......... HindIII(703) .........

661 gacgctaacc tggccggctc tggatccggt gatgacgatg acaagctcgc ccttaagggc 720

661 ctgcgattgg accggccgag acctaggcca ctactgctac tgttcgagcg ggaattcccg 720

His_patch_thio ...... .......... EK .......... V5

.......... .......... .......... .......... .......... ..........

721 gagcttgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt 780

721 ctcgaacttc cattcggata gggattggga gaggagccag agctaagatg cgcatggcca 780

.......... V5 .......... .......... .......... 6xHis

.......... .......... .......... .......... .......... ..........

781 catcatcacc atcaccattg agtttaaacg gtctccagct tggctgtttt ggcggatgag 840

781 gtagtagtgg tagtggtaac tcaaatttgc cagaggtcga accgacaaaa ccgcctactc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga 900

841 tcttctaaaa gtcggactat gtctaattta gtcttgcgtc ttcgccagac tattttgtct 900

pBAD_rev_pri pTrcHis_rev_pri .. .......... .......... rrnB_term

.......... .......... .......... .......... .......... ..........

901 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga 960

901 taaacggacc gccgtcatcg cgccaccagg gtggactggg gtacggcttg agtcttcact 960

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg 1020

961 ttgcggcatc gcggctacca tcacacccca gaggggtacg ctctcatccc ttgacggtcc 1020

.......... .......... rrnB_term .......... ......... rrnB_T1_term

.......... .......... .......... .......... .......... ..........

1021 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1080

1021 gtagtttatt ttgctttccg agtcagcttt ctgacccgga aagcaaaata gacaacaaac 1080

.... rrnB_term rrnB_T1_term .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 1140

1081 agccacttgc gagaggactc atcctgttta ggcggccctc gcctaaactt gcaacgcttc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag 1200

1141 gttgccgggc ctcccaccgc ccgtcctgcg ggcggtattt gacggtccgt agtttaattc 1200

.......... .......... .......... .......... ......... rrnB_T2_term

.......... .......... .......... .......... .......... ..........

1201 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt tgtttatttt 1260

1201 gtcttccggt aggactgcct accggaaaaa cgcaaagatg tttgagaaaa acaaataaaa 1260

rrnB_T2_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1320

1261 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1320

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1380

1321 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1380

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

1381 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1440

1381 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1500

1441 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 1500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 1560

1501 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 1560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 1620

1561 atacaccgcg ccataatagg gcacaactgc ggcccgttct cgttgagcca gcggcgtatg 1620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 1680

1621 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 1680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 1740

1681 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 1740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 1800

1741 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 1800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 1860

1801 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 1860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 1920

1861 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 1920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... AseI(1935) .......... .......... .......... ..........

1921 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 1980

1921 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 1980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2040

1981 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2100

2041 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 2160

2101 gggcatagca tcaatagatg tgctgcccct cagtccgttg atacctactt gctttatctg 2160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2220

2161 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2220

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2280

2221 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2340

2281 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2400

2341 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2460

2401 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 2520

2461 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacagg 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 2580

2521 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 2640

2581 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 2700

2641 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 2760

2701 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 2820

2761 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 2880

2821 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 2940

2881 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3000

2941 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 3000

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3060

3001 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3120

3061 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NdeI(3177)

3121 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 3180

3121 gtcactcgct ccttcgcctt ctcgcggact acgccataaa agaggaatgc gtagacacgc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 3240

3181 cataaagtgt ggcgtatacc acgtgagagt catgttagac gagactacgg cgtatcaatt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 3300

3241 cggtcatatg tgaggcgata gcgatgcact gacccagtac cgacgcgggg ctgtgggcgg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 3360

3301 ttgtgggcga ctgcgcggga ctgcccgaac agacgagggc cgtaggcgaa tgtctgttcg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 3420

3361 acactggcag aggccctcga cgtacacagt ctccaaaagt ggcagtagtg gctttgcgcg 3420

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat 3480

3421 ctccgtcgtc tagttaagcg cgcgcttccg cttcgccgta cgtattacac ggacagttta 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt 3540

3481 cctgcttcgt ccctaagacg tttgggatac gatgaggcag ttcggcagtt aacagactaa 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg 3600

3541 gcaatggtta atactgttga actgccgatg tagtaagtga aaaagaagtg ttggccgtgc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc 3660

3601 cttgagcgag cccgaccggg gccacgtaaa aaatttatgg gcgctcttta tctcaactag 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag 3720

3661 cagttttggt tgtaacgctg gctgccaccg ctatccgtag gcccaccacg agttttcgtc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg 3780

3721 gaagcggacc gactatgcaa ccaggagcgc ggtcgaattc tgcgattagg gattgacgac 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRV(3822) .........

3781 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat 3840

3781 cgccttttct acactgtctg cgctgccgct gttcgtttgt acgacacgct gcgaccgcta 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt 3900

3841 tagttttaac gacagacggt ccactagcga ctacatgact gttcggagcg catgggctaa 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc 3960

3901 taggtagcca cctacctcgc tgagcaatta gcgaaggtac gcggcgtcat tgttaacgag 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat 4020

3961 ttcgtctaaa tagcggtcgt cgaggcttat cgcgggaagg ggaacgggcc gcaattacta 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt 4080

4021 aacgggtttg tccagcgact ttacgccgac cacgcgaagt aggcccgctt tcttggggca 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta 4140

4081 taaccgttta taactgccgg tcaattcggt aagtacggtc atccgcgcgc ctgctttcat 4140

.......... .......... .......... .......... .......... ..........

NruI(4144) .......... .......... .......... .......... ..........

4141 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc 4200

4141 ttgggtgacc actatggtaa gcgctcggag gcctactgct ggcatcacta cttagagagg 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac 4260

4201 accgcccttg tcgttttata gtgggccagc cgtttgttta agagcaggga ctaaaaagtg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc 4320

4261 gtgggggact ggcgcttacc actctaactc ttatattgga aagtaagggt cgccagccag 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc 4380

4321 ctattttttt agctctattg gcaaccggag ttagccgcaa tttgggcggt ggtctacccg 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact 4440

4381 taatttgctc atagggccgt cgtcccctag taaaacgcga agtcggtatg aaaagtatga 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cccgccattc agag...... .......... .......... .......... .......... 4454

4441 gggcggtaag tctc...... .......... .......... .......... .......... 4454

.......... .......... .......... .......... .......... ..........