Sequence of pBAD_myc-His

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Sequence of pBAD_myc-His_A

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Sequence of pBAD/myc-His A : 4094 BP; 1037 A; 1052 C; 1016 G; 989 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... AgeI(74) .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaacccg 300

AraI1I2 .......... ARA_pro .......... .......... ..........

NcoI XhoI,SacI,BglII PstI42),PvuII(343) KpnI(350)

301 taacaggagg aattaaccat ggatccgagc tcgagatctg cagctggtac catatgggaa 360

301 attgtcctcc ttaattggta cctaggctcg agctctagac gtcgaccatg gtataccctt 360

.......... .......... .......... .......... .......... ..........

BstBI(362),HindIII(365) ApaI(375) ...... .......... SalI(416)

361 ttcgaagctt gggcccgaac aaaaactcat ctcagaagag gatctgaata gcgccgtcga 420

361 aagcttcgaa cccgggcttg tttttgagta gagtcttctc ctagacttat cgcggcagct 420

.......... .......... .......... c_myc .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccatcatcat catcatcatt gagtttaaac ggtctccagc ttggctgttt tggcggatga 480

421 ggtagtagta gtagtagtaa ctcaaatttg ccagaggtcg aaccgacaaa accgcctact 480

6xHis .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct gataaaacag 540

481 ctcttctaaa agtcggacta tgtctaattt agtcttgcgt cttcgccaga ctattttgtc 540

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

541 aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg 600

541 ttaaacggac cgccgtcatc gcgccaccag ggtggactgg ggtacggctt gagtcttcac 600

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

601 aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag 660

601 tttgcggcat cgcggctacc atcacacccc agaggggtac gctctcatcc cttgacggtc 660

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 720

661 cgtagtttat tttgctttcc gagtcagctt tctgacccgg aaagcaaaat agacaacaaa 720

.......... .... rrnB_term rrnB_T1_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa 780

721 cagccacttg cgagaggact catcctgttt aggcggccct cgcctaaact tgcaacgctt 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa 840

781 cgttgccggg cctcccaccg cccgtcctgc gggcggtatt tgacggtccg tagtttaatt 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt 900

841 cgtcttccgg taggactgcc taccggaaaa acgcaaagat gtttgagaaa acaaataaaa 900

......... rrnB_T2_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 960

901 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 960

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1020

961 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1020

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

1021 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 1080

1021 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 1080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 1140

1081 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 1140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 1200

1141 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 1200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 1260

1201 atacaccgcg ccataatagg gcacaactgc ggcccgttct cgttgagcca gcggcgtatg 1260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 1320

1261 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 1320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 1380

1321 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 1380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 1440

1381 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 1440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 1500

1441 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 1500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 1560

1501 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 1560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... AseI(1593) .......... ..........

1561 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 1620

1561 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 1620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 1680

1621 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 1680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 1740

1681 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 1740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1800

1741 gggcatagca tcaatagatg tgctgcccct cagtccgttg atacctactt gctttatctg 1800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1860

1801 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 1860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1920

1861 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1980

1921 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 2040

1981 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 2040

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2041 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 2100

2041 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 2160

2101 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacagg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 2220

2161 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 2280

2221 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 2340

2281 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 2400

2341 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 2460

2401 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 2520

2461 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 2580

2521 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 2640

2581 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 2640

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 2700

2641 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 2760

2701 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 2820

2761 gtcactcgct ccttcgcctt ctcgcggact acgccataaa agaggaatgc gtagacacgc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 2880

2821 cataaagtgt ggcgtatacc acgtgagagt catgttagac gagactacgg cgtatcaatt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 2940

2881 cggtcatatg tgaggcgata gcgatgcact gacccagtac cgacgcgggg ctgtgggcgg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 3000

2941 ttgtgggcga ctgcgcggga ctgcccgaac agacgagggc cgtaggcgaa tgtctgttcg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 3060

3001 acactggcag aggccctcga cgtacacagt ctccaaaagt ggcagtagtg gctttgcgcg 3060

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat 3120

3061 ctccgtcgtc tagttaagcg cgcgcttccg cttcgccgta cgtattacac ggacagttta 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggacgaagca gggattctgc aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt 3180

3121 cctgcttcgt ccctaagacg tttgggatac gatgaggcag ttcggcagtt aacagactaa 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgttaccaat tatgacaact tgacggctac atcattcact ttttcttcac aaccggcacg 3240

3181 gcaatggtta atactgttga actgccgatg tagtaagtga aaaagaagtg ttggccgtgc 3240

.......... .......... .......... AraC .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaactcgctc gggctggccc cggtgcattt tttaaatacc cgcgagaaat agagttgatc 3300

3241 cttgagcgag cccgaccggg gccacgtaaa aaatttatgg gcgctcttta tctcaactag 3300

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gtcaaaacca acattgcgac cgacggtggc gataggcatc cgggtggtgc tcaaaagcag 3360

3301 cagttttggt tgtaacgctg gctgccaccg ctatccgtag gcccaccacg agttttcgtc 3360

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cttcgcctgg ctgatacgtt ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg 3420

3361 gaagcggacc gactatgcaa ccaggagcgc ggtcgaattc tgcgattagg gattgacgac 3420

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... ......... EcoRV(3480)

3421 gcggaaaaga tgtgacagac gcgacggcga caagcaaaca tgctgtgcga cgctggcgat 3480

3421 cgccttttct acactgtctg cgctgccgct gttcgtttgt acgacacgct gcgaccgcta 3480

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atcaaaattg ctgtctgcca ggtgatcgct gatgtactga caagcctcgc gtacccgatt 3540

3481 tagttttaac gacagacggt ccactagcga ctacatgact gttcggagcg catgggctaa 3540

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 atccatcggt ggatggagcg actcgttaat cgcttccatg cgccgcagta acaattgctc 3600

3541 taggtagcca cctacctcgc tgagcaatta gcgaaggtac gcggcgtcat tgttaacgag 3600

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aagcagattt atcgccagca gctccgaata gcgcccttcc ccttgcccgg cgttaatgat 3660

3601 ttcgtctaaa tagcggtcgt cgaggcttat cgcgggaagg ggaacgggcc gcaattacta 3660

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttgcccaaac aggtcgctga aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt 3720

3661 aacgggtttg tccagcgact ttacgccgac cacgcgaagt aggcccgctt tcttggggca 3720

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 attggcaaat attgacggcc agttaagcca ttcatgccag taggcgcgcg gacgaaagta 3780

3721 taaccgttta taactgccgg tcaattcggt aagtacggtc atccgcgcgc ctgctttcat 3780

.......... .......... AraC .......... .......... ..........

.......... .......... NruI(3802) .......... .......... ..........

3781 aacccactgg tgataccatt cgcgagcctc cggatgacga ccgtagtgat gaatctctcc 3840

3781 ttgggtgacc actatggtaa gcgctcggag gcctactgct ggcatcacta cttagagagg 3840

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tggcgggaac agcaaaatat cacccggtcg gcaaacaaat tctcgtccct gatttttcac 3900

3841 accgcccttg tcgttttata gtgggccagc cgtttgttta agagcaggga ctaaaaagtg 3900

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 caccccctga ccgcgaatgg tgagattgag aatataacct ttcattccca gcggtcggtc 3960

3901 gtgggggact ggcgcttacc actctaactc ttatattgga aagtaagggt cgccagccag 3960

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gataaaaaaa tcgagataac cgttggcctc aatcggcgtt aaacccgcca ccagatgggc 4020

3961 ctattttttt agctctattg gcaaccggag ttagccgcaa tttgggcggt ggtctacccg 4020

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 attaaacgag tatcccggca gcaggggatc attttgcgct tcagccatac ttttcatact 4080

4021 taatttgctc atagggccgt cgtcccctag taaaacgcga agtcggtatg aaaagtatga 4080

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cccgccattc agag...... .......... .......... .......... .......... 4094

4081 gggcggtaag tctc...... .......... .......... .......... .......... 4094

.......... .......... .......... .......... .......... ..........