Sequence of pBAD_myc-His

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Sequence of pBAD_myc-His_C

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Sequence of pBAD/myc-His C : 4093 BP; 1039 A; 1050 C; 1014 G; 990 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct 60

1 ttctttggtt aacaggtata acgtagtctg taacggcagt gacgcagaaa atgaccgaga 60

.......... araO2 .......... .......... .......... ..........

.......... AgeI(74) .......... .......... .......... ..........

61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca 120

61 agagcgattg gtttggccat tggggcgaat aattttcgta agacattgtt tcgccctggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg 180

121 ttcggtactg tttttgcgca ttgttttcac agatattagt gccgtctttt caggtgtaac 180

.......... .......... .......... .......... araO1 ..........

.......... .......... .......... .......... .......... ..........

181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg 240

181 taataaacgt gccgcagtgt gaaacgatac ggtatcgtaa aaataggtat tctaatcgcc 240

.......... ......... CAP_BS pBAD_fwd_pri AraI1I2 .........

.......... .......... .......... .......... .......... ..........

241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc 300

241 taggatggac tgcgaaaaat agcgttgaga gatgacaaag aggtatgggc aaaaaacccg 300

AraI1I2 .......... ARA_pro .......... .......... ..........

NcoI XhoI,SacI,BglII PstI42),PvuII(343) KpnI(350)

301 taacaggagg aattaaccat ggatccgagc tcgagatctg cagctggtac catatgggaa 360

301 attgtcctcc ttaattggta cctaggctcg agctctagac gtcgaccatg gtataccctt 360

.......... .......... .......... .......... .......... ..........

BstBI(362),HindIII(365) ...... .......... .......... SalI(415)

361 ttcgaagctt acgtagaaca aaaactcatc tcagaagagg atctgaatag cgccgtcgac 420

361 aagcttcgaa tgcatcttgt ttttgagtag agtcttctcc tagacttatc gcggcagctg 420

.......... .......... c_myc .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 catcatcatc atcatcattg agtttaaacg gtctccagct tggctgtttt ggcggatgag 480

421 gtagtagtag tagtagtaac tcaaatttgc cagaggtcga accgacaaaa ccgcctactc 480

6xHis .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga 540

481 tcttctaaaa gtcggactat gtctaattta gtcttgcgtc ttcgccagac tattttgtct 540

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

541 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga 600

541 taaacggacc gccgtcatcg cgccaccagg gtggactggg gtacggcttg agtcttcact 600

.......... .......... .......... rrnB_term .......... ..........

.......... .......... .......... .......... .......... ..........

601 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg 660

601 ttgcggcatc gcggctacca tcacacccca gaggggtacg ctctcatccc ttgacggtcc 660

.......... .......... rrnB_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 720

661 gtagtttatt ttgctttccg agtcagcttt ctgacccgga aagcaaaata gacaacaaac 720

.......... .... rrnB_term rrnB_T1_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 780

721 agccacttgc gagaggactc atcctgttta ggcggccctc gcctaaactt gcaacgcttc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag 840

781 gttgccgggc ctcccaccgc ccgtcctgcg ggcggtattt gacggtccgt agtttaattc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt 900

841 gtcttccggt aggactgcct accggaaaaa cgcaaagatg tttgagaaaa caaataaaaa 900

......... rrnB_T2_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata 960

901 gatttatgta agtttataca taggcgagta ctctgttatt gggactattt acgaagttat 960

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt 1020

961 tataactttt tccttctcat actcataagt tgtaaaggca cagcgggaat aagggaaaaa 1020

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

1021 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc 1080

1021 acgccgtaaa acggaaggac aaaaacgagt gggtctttgc gaccactttc attttctacg 1080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat 1140

1081 acttctagtc aacccacgtg ctcacccaat gtagcttgac ctagagttgt cgccattcta 1140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct 1200

1141 ggaactctca aaagcggggc ttcttgcaaa aggttactac tcgtgaaaat ttcaagacga 1200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca 1260

1201 tacaccgcgc cataataggg cacaactgcg gcccgttctc gttgagccag cggcgtatgt 1260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg 1320

1261 gataagagtc ttactgaacc aactcatgag tggtcagtgt cttttcgtag aatgcctacc 1320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa 1380

1321 gtactgtcat tctcttaata cgtcacgacg gtattggtac tcactattgt gacgccggtt 1380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg 1440

1381 gaatgaagac tgttgctagc ctcctggctt cctcgattgg cgaaaaaacg tgttgtaccc 1440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga 1500

1441 cctagtacat tgagcggaac tagcaaccct tggcctcgac ttacttcggt atggtttgct 1500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg 1560

1501 gctcgcactg tggtgctacg gacatcgtta ccgttgttgc aacgcgtttg ataattgacc 1560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... AseI(1592) .......... ..........

1561 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt 1620

1561 gcttgatgaa tgagatcgaa gggccgttgt taattatctg acctacctcc gcctatttca 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg 1680

1621 acgtcctggt gaagacgcga gccgggaagg ccgaccgacc aaataacgac tatttagacc 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc 1740

1681 tcggccactc gcacccagag cgccatagta acgtcgtgac cccggtctac cattcgggag 1740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca 1800

1741 ggcatagcat caatagatgt gctgcccctc agtccgttga tacctacttg ctttatctgt 1800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc 1860

1801 ctagcgactc tatccacgga gtgactaatt cgtaaccatt gacagtctgg ttcaaatgag 1860

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat 1920

1861 tatatatgaa atctaactaa attttgaagt aaaaattaaa ttttcctaga tccacttcta 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc 1980

1921 ggaaaaacta ttagagtact ggttttaggg aattgcactc aaaagcaagg tgactcgcag 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg 2040

1981 tctggggcat cttttctagt ttcctagaag aactctagga aaaaaagacg cgcattagac 2040

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 2100

2041 gacgaacgtt tgtttttttg gtggcgatgg tcgccaccaa acaaacggcc tagttctcga 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 2160

2101 tggttgagaa aaaggcttcc attgaccgaa gtcgtctcgc gtctatggtt tatgacagga 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 2220

2161 agatcacatc ggcatcaatc cggtggtgaa gttcttgaga catcgtggcg gatgtatgga 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 2280

2221 gcgagacgat taggacaatg gtcaccgacg acggtcaccg ctattcagca cagaatggcc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 2340

2281 caacctgagt tctgctatca atggcctatt ccgcgtcgcc agcccgactt gccccccaag 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 2400

2341 cacgtgtgtc gggtcgaacc tcgcttgctg gatgtggctt gactctatgg atgtcgcact 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 2460

2401 cgatactctt tcgcggtgcg aagggcttcc ctctttccgc ctgtccatag gccattcgcc 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2520

2461 gtcccagcct tgtcctctcg cgtgctccct cgaaggtccc cctttgcgga ccatagaaat 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2580

2521 atcaggacag cccaaagcgg tggagactga actcgcagct aaaaacacta cgagcagtcc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2640

2581 ccccgcctcg gatacctttt tgcggtcgtt gcgccggaaa aatgccaagg accggaaaac 2640

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2700

2641 gaccggaaaa cgagtgtaca agaaaggacg caatagggga ctaagacacc tattggcata 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2760

2701 atggcggaaa ctcactcgac tatggcgagc ggcgtcggct tgctggctcg cgtcgctcag 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg 2820

2761 tcactcgctc cttcgccttc tcgcggacta cgccataaaa gaggaatgcg tagacacgcc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag 2880

2821 ataaagtgtg gcgtatacca cgtgagagtc atgttagacg agactacggc gtatcaattc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca 2940

2881 ggtcatatgt gaggcgatag cgatgcactg acccagtacc gacgcggggc tgtgggcggt 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 3000

2941 tgtgggcgac tgcgcgggac tgcccgaaca gacgagggcc gtaggcgaat gtctgttcga 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 3060

3001 cactggcaga ggccctcgac gtacacagtc tccaaaagtg gcagtagtgg ctttgcgcgc 3060

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aggcagcaga tcaattcgcg cgcgaaggcg aagcggcatg cataatgtgc ctgtcaaatg 3120

3061 tccgtcgtct agttaagcgc gcgcttccgc ttcgccgtac gtattacacg gacagtttac 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gacgaagcag ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc 3180

3121 ctgcttcgtc cctaagacgt ttgggatacg atgaggcagt tcggcagtta acagactaag 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gttaccaatt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg 3240

3181 caatggttaa tactgttgaa ctgccgatgt agtaagtgaa aaagaagtgt tggccgtgcc 3240

.......... .......... .......... AraC .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg 3300

3241 ttgagcgagc ccgaccgggg ccacgtaaaa aatttatggg cgctctttat ctcaactagc 3300

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc 3360

3301 agttttggtt gtaacgctgg ctgccaccgc tatccgtagg cccaccacga gttttcgtcg 3360

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg 3420

3361 aagcggaccg actatgcaac caggagcgcg gtcgaattct gcgattaggg attgacgacc 3420

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... ......... EcoRV(3479)

3421 cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata 3480

3421 gccttttcta cactgtctgc gctgccgctg ttcgtttgta cgacacgctg cgaccgctat 3480

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta 3540

3481 agttttaacg acagacggtc cactagcgac tacatgactg ttcggagcgc atgggctaat 3540

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca 3600

3541 aggtagccac ctacctcgct gagcaattag cgaaggtacg cggcgtcatt gttaacgagt 3600

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt 3660

3601 tcgtctaaat agcggtcgtc gaggcttatc gcgggaaggg gaacgggccg caattactaa 3660

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta 3720

3661 acgggtttgt ccagcgactt tacgccgacc acgcgaagta ggcccgcttt cttggggcat 3720

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa 3780

3721 aaccgtttat aactgccggt caattcggta agtacggtca tccgcgcgcc tgctttcatt 3780

.......... .......... AraC .......... .......... ..........

.......... .......... NruI(3801) .......... .......... ..........

3781 acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct 3840

3781 tgggtgacca ctatggtaag cgctcggagg cctactgctg gcatcactac ttagagagga 3840

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc 3900

3841 ccgcccttgt cgttttatag tgggccagcc gtttgtttaa gagcagggac taaaaagtgg 3900

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg 3960

3901 tgggggactg gcgcttacca ctctaactct tatattggaa agtaagggtc gccagccagc 3960

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca 4020

3961 tattttttta gctctattgg caaccggagt tagccgcaat ttgggcggtg gtctacccgt 4020

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc 4080

4021 aatttgctca tagggccgtc gtcccctagt aaaacgcgaa gtcggtatga aaagtatgag 4080

.......... .......... AraC .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccgccattca gag....... .......... .......... .......... .......... 4093

4081 ggcggtaagt ctc....... .......... .......... .......... .......... 4093

.......... .......... .......... .......... .......... ..........