5LOCUS pBAD/myc-His/LacZ 7241 bp DNA SYN 22-Oct-2007 DEFINITION pBAD/myc-His/LacZ ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..7241 /organism="pBAD/myc-His/LacZ" /mol_type="other DNA" CDS complement(6337..25) /label="ORF frame 1" /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS*" misc_feature 4..19 /label="araO2" misc_feature 161..172 /label="araO1" misc_feature 203..216 /label="CAP_BS" misc_feature 208..227 /label="pBAD_fwd_primer" misc_feature 213..251 /label="AraI1I2" promoter 248..276 /label="ARA_promoter" misc_feature complement(387..409) /label="M13_pUC_fwd_primer" gene 385..3429 /label="lacZ" /gene="lacZ" CDS 319..3588 /label="lacZ" /gene="lacZ" /note="ORF frame 1" /translation="MDPLVTAASVLEFGLFETMIDPVVLQRRDWENPGVTQLNRLAAH PPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPVPEAVPESWLECDLPEADTVVVPS NWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGV NSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWR MSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQ GETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADG TLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILL MKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAM SERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTT ATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKY WQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFAD RTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEV PLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENL SVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQF TRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAW QHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGL GPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFN ISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHY QLVWCQKAAASRILQISITLAAARDLQLVPYGNSKLGPEQKLISEEDLNSAVDHHHHH H*" misc_feature 3523..3552 /label="c_myc" misc_feature 3568..3585 /label="6xHis" misc_feature complement(3641..3658) /label="pBAD_rev_primer" misc_feature complement(3641..3658) /label="pTrcHis_rev_primer" terminator 3691..3848 /label="rrnB_terminator" terminator 3814..3857 /label="rrnB_T1_terminator" terminator 3989..4016 /label="rrnB_T2_terminator" promoter 4058..4086 /label="AmpR_promoter" CDS 4128..4988 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 4128..4988 /label="Ampicillin" /gene="Ampicillin" rep_origin 5143..5762 /label="pBR322_origin" misc_feature 6159..6181 /label="pGEX_3_primer" misc_feature complement(6337..7215) /label="AraC" ORIGIN 1 AAGAAACCAA TTGTCCATAT TGCATCAGAC ATTGCCGTCA CTGCGTCTTT TACTGGCTCT 61 TCTCGCTAAC CAAACCGGTA ACCCCGCTTA TTAAAAGCAT TCTGTAACAA AGCGGGACCA 121 AAGCCATGAC AAAAACGCGT AACAAAAGTG TCTATAATCA CGGCAGAAAA GTCCACATTG 181 ATTATTTGCA CGGCGTCACA CTTTGCTATG CCATAGCATT TTTATCCATA AGATTAGCGG 241 ATCCTACCTG ACGCTTTTTA TCGCAACTCT CTACTGTTTC TCCATACCCG TTTTTTGGGC 301 TAACAGGAGG AATTAACCAT GGATCCACTA GTAACGGCCG CCAGTGTGCT GGAATTCGGC 361 TTGTTCGAAA CGATGATAGA TCCCGTCGTT TTACAACGTC GTGACTGGGA AAACCCTGGC 421 GTTACCCAAC TTAATCGCCT TGCAGCACAT CCCCCTTTCG CCAGCTGGCG TAATAGCGAA 481 GAGGCCCGCA CCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGCGCTTT 541 GCCTGGTTTC CGGTACCAGA AGCGGTGCCG GAAAGCTGGC TGGAGTGCGA TCTTCCTGAG 601 GCCGATACTG TCGTCGTCCC CTCAAACTGG CAGATGCACG GTTACGATGC GCCCATCTAC 661 ACCAACGTAA CCTATCCCAT TACGGTCAAT CCGCCGTTTG TTCCCACGGA GAATCCGACG 721 GGTTGTTACT CGCTCACATT TAATGTTGAT GAAAGCTGGC TACAGGAAGG CCAGACGCGA 781 ATTATTTTTG ATGGCGTTAA CTCGGCGTTT CATCTGTGGT GCAACGGGCG CTGGGTCGGT 841 TACGGCCAGG ACAGTCGTTT GCCGTCTGAA TTTGACCTGA GCGCATTTTT ACGCGCCGGA 901 GAAAACCGCC TCGCGGTGAT GGTGCTGCGT TGGAGTGACG GCAGTTATCT GGAAGATCAG 961 GATATGTGGC GGATGAGCGG CATTTTCCGT GACGTCTCGT TGCTGCATAA ACCGACTACA 1021 CAAATCAGCG ATTTCCATGT TGCCACTCGC TTTAATGATG ATTTCAGCCG CGCTGTACTG 1081 GAGGCTGAAG TTCAGATGTG CGGCGAGTTG CGTGACTACC TACGGGTAAC AGTTTCTTTA 1141 TGGCAGGGTG AAACGCAGGT CGCCAGCGGC ACCGCGCCTT TCGGCGGTGA AATTATCGAT 1201 GAGCGTGGTG GTTATGCCGA TCGCGTCACA CTACGTCTGA ACGTCGAAAA CCCGAAACTG 1261 TGGAGCGCCG AAATCCCGAA TCTCTATCGT GCGGTGGTTG AACTGCACAC CGCCGACGGC 1321 ACGCTGATTG AAGCAGAAGC CTGCGATGTC GGTTTCCGCG AGGTGCGGAT TGAAAATGGT 1381 CTGCTGCTGC TGAACGGCAA GCCGTTGCTG ATTCGAGGCG TTAACCGTCA CGAGCATCAT 1441 CCTCTGCATG GTCAGGTCAT GGATGAGCAG ACGATGGTGC AGGATATCCT GCTGATGAAG 1501 CAGAACAACT TTAACGCCGT GCGCTGTTCG CATTATCCGA ACCATCCGCT GTGGTACACG 1561 CTGTGCGACC GCTACGGCCT GTATGTGGTG GATGAAGCCA ATATTGAAAC CCACGGCATG 1621 GTGCCAATGA ATCGTCTGAC CGATGATCCG CGCTGGCTAC CGGCGATGAG CGAACGCGTA 1681 ACGCGAATGG TGCAGCGCGA TCGTAATCAC CCGAGTGTGA TCATCTGGTC GCTGGGGAAT 1741 GAATCAGGCC ACGGCGCTAA TCACGACGCG CTGTATCGCT GGATCAAATC TGTCGATCCT 1801 TCCCGCCCGG TGCAGTATGA AGGCGGCGGA GCCGACACCA CGGCCACCGA TATTATTTGC 1861 CCGATGTACG CGCGCGTGGA TGAAGACCAG CCCTTCCCGG CTGTGCCGAA ATGGTCCATC 1921 AAAAAATGGC TTTCGCTACC TGGAGAGACG CGCCCGCTGA TCCTTTGCGA ATACGCCCAC 1981 GCGATGGGTA ACAGTCTTGG CGGTTTCGCT AAATACTGGC AGGCGTTTCG TCAGTATCCC 2041 CGTTTACAGG GCGGCTTCGT CTGGGACTGG GTGGATCAGT CGCTGATTAA ATATGATGAA 2101 AACGGCAACC CGTGGTCGGC TTACGGCGGT GATTTTGGCG ATACGCCGAA CGATCGCCAG 2161 TTCTGTATGA ACGGTCTGGT CTTTGCCGAC CGCACGCCGC ATCCAGCGCT GACGGAAGCA 2221 AAACACCAGC AGCAGTTTTT CCAGTTCCGT TTATCCGGGC AAACCATCGA AGTGACCAGC 2281 GAATACCTGT TCCGTCATAG CGATAACGAG CTCCTGCACT GGATGGTGGC GCTGGATGGT 2341 AAGCCGCTGG CAAGCGGTGA AGTGCCTCTG GATGTCGCTC CACAAGGTAA ACAGTTGATT 2401 GAACTGCCTG AACTACCGCA GCCGGAGAGC GCCGGGCAAC TCTGGCTCAC AGTACGCGTA 2461 GTGCAACCGA ACGCGACCGC ATGGTCAGAA GCCGGGCACA TCAGCGCCTG GCAGCAGTGG 2521 CGTCTGGCGG AAAACCTCAG TGTGACGCTC CCCGCCGCGT CCCACGCCAT CCCGCATCTG 2581 ACCACCAGCG AAATGGATTT TTGCATCGAG CTGGGTAATA AGCGTTGGCA ATTTAACCGC 2641 CAGTCAGGCT TTCTTTCACA GATGTGGATT GGCGATAAAA AACAACTGCT GACGCCGCTG 2701 CGCGATCAGT TCACCCGTGC ACCGCTGGAT AACGACATTG GCGTAAGTGA AGCGACCCGC 2761 ATTGACCCTA ACGCCTGGGT CGAACGCTGG AAGGCGGCGG GCCATTACCA GGCCGAAGCA 2821 GCGTTGTTGC AGTGCACGGC AGATACACTT GCTGATGCGG TGCTGATTAC GACCGCTCAC 2881 GCGTGGCAGC ATCAGGGGAA AACCTTATTT ATCAGCCGGA AAACCTACCG GATTGATGGT 2941 AGTGGTCAAA TGGCGATTAC CGTTGATGTT GAAGTGGCGA GCGATACACC GCATCCGGCG 3001 CGGATTGGCC TGAACTGCCA GCTGGCGCAG GTAGCAGAGC GGGTAAACTG GCTCGGATTA 3061 GGGCCGCAAG AAAACTATCC CGACCGCCTT ACTGCCGCCT GTTTTGACCG CTGGGATCTG 3121 CCATTGTCAG ACATGTATAC CCCGTACGTC TTCCCGAGCG AAAACGGTCT GCGCTGCGGG 3181 ACGCGCGAAT TGAATTATGG CCCACACCAG TGGCGCGGCG ACTTCCAGTT CAACATCAGC 3241 CGCTACAGTC AACAGCAACT GATGGAAACC AGCCATCGCC ATCTGCTGCA CGCGGAAGAA 3301 GGCACATGGC TGAATATCGA CGGTTTCCAT ATGGGGATTG GTGGCGACGA CTCCTGGAGC 3361 CCGTCAGTAT CGGCGGAATT CCAGCTGAGC GCCGGTCGCT ACCATTACCA GTTGGTCTGG 3421 TGTCAAAAAG CGGCCGCAAG CCGAATTCTG CAGATATCCA TCACACTGGC GGCCGCTCGA 3481 GATCTGCAGC TGGTACCATA TGGGAATTCG AAGCTTGGGC CCGAACAAAA ACTCATCTCA 3541 GAAGAGGATC TGAATAGCGC CGTCGACCAT CATCATCATC ATCATTGAGT TTAAACGGTC 3601 TCCAGCTTGG CTGTTTTGGC GGATGAGAGA AGATTTTCAG CCTGATACAG ATTAAATCAG 3661 AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG CAGTAGCGCG GTGGTCCCAC 3721 CTGACCCCAT GCCGAACTCA GAAGTGAAAC GCCGTAGCGC CGATGGTAGT GTGGGGTCTC 3781 CCCATGCGAG AGTAGGGAAC TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC 3841 TGGGCCTTTC GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG 3901 CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC AGGACGCCCG 3961 CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC TGACGGATGG CCTTTTTGCG 4021 TTTCTACAAA CTCTTTTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA 4081 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC 4141 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC 4201 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA 4261 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC 4321 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG 4381 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC 4441 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA 4501 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG 4561 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC 4621 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG 4681 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT 4741 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG 4801 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG 4861 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC 4921 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC 4981 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT 5041 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT 5101 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT 5161 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG 5221 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA 5281 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA 5341 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG 5401 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG 5461 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT 5521 ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA 5581 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC 5641 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG 5701 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG 5761 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT 5821 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC 5881 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC 5941 GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA 6001 CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG 6061 GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT 6121 GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG 6181 GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCAGATC AATTCGCGCG CGAAGGCGAA 6241 GCGGCATGCA TAATGTGCCT GTCAAATGGA CGAAGCAGGG ATTCTGCAAA CCCTATGCTA 6301 CTCCGTCAAG CCGTCAATTG TCTGATTCGT TACCAATTAT GACAACTTGA CGGCTACATC 6361 ATTCACTTTT TCTTCACAAC CGGCACGGAA CTCGCTCGGG CTGGCCCCGG TGCATTTTTT 6421 AAATACCCGC GAGAAATAGA GTTGATCGTC AAAACCAACA TTGCGACCGA CGGTGGCGAT 6481 AGGCATCCGG GTGGTGCTCA AAAGCAGCTT CGCCTGGCTG ATACGTTGGT CCTCGCGCCA 6541 GCTTAAGACG CTAATCCCTA ACTGCTGGCG GAAAAGATGT GACAGACGCG ACGGCGACAA 6601 GCAAACATGC TGTGCGACGC TGGCGATATC AAAATTGCTG TCTGCCAGGT GATCGCTGAT 6661 GTACTGACAA GCCTCGCGTA CCCGATTATC CATCGGTGGA TGGAGCGACT CGTTAATCGC 6721 TTCCATGCGC CGCAGTAACA ATTGCTCAAG CAGATTTATC GCCAGCAGCT CCGAATAGCG 6781 CCCTTCCCCT TGCCCGGCGT TAATGATTTG CCCAAACAGG TCGCTGAAAT GCGGCTGGTG 6841 CGCTTCATCC GGGCGAAAGA ACCCCGTATT GGCAAATATT GACGGCCAGT TAAGCCATTC 6901 ATGCCAGTAG GCGCGCGGAC GAAAGTAAAC CCACTGGTGA TACCATTCGC GAGCCTCCGG 6961 ATGACGACCG TAGTGATGAA TCTCTCCTGG CGGGAACAGC AAAATATCAC CCGGTCGGCA 7021 AACAAATTCT CGTCCCTGAT TTTTCACCAC CCCCTGACCG CGAATGGTGA GATTGAGAAT 7081 ATAACCTTTC ATTCCCAGCG GTCGGTCGAT AAAAAAATCG AGATAACCGT TGGCCTCAAT 7141 CGGCGTTAAA CCCGCCACCA GATGGGCATT AAACGAGTAT CCCGGCAGCA GGGGATCATT 7201 TTGCGCTTCA GCCATACTTT TCATACTCCC GCCATTCAGA G //IpBAD/myc-His/LacZ "Times New Roman#CIORF 1"Times New Roman(CIaraO2"Times New RomanCɆIaraO1"Times New RomanICAP_BS"Times New RomanCI pBAD_fwd_pri"Times New RomanCIAraI1I2"Times New Roman*CɆIARA_pro"Times New RomanCI?ORF 1"Times New RomanCIe lacZ"Times New RomanIM13_pUC_fwd_pri"Times New Roman)CɆI c_myc"Times New RomanI 6xHis"Times New RomanCI9J pBAD_rev_pri"Times New RomanCI9JpTrcHis_rev_pri"Times New RomanCɆIk rrnB_term"Times New RomanI rrnB_T1_term"Times New RomanCI rrnB_T2_term"Times New RomanCIAmpR_pro"Times New RomanCɆI |ORF 3"Times New RomanI | Ampicillin"Times New RomanCI pBR322_origin"Times New RomanCI% pGEX_3_pri"Times New RomanCɆI/AraC"Times New Roman AgeI"Times New RomanJI NcoI(317),SpeI(327)"Times New Roman=I %55r AatII"Times New RomanIG9++ ClaI"Times New RomanIg]RCR BclI"Times New RomanI, SacI"Times New Roman I;CKDR :XhoI(3476),BglII(3480),HindIII(3511),ApaI(3521),SalI(3562)"Times New Roman Ii2 AseI"Times New RomanI(amxx AflII"Times New RomanIa!UI I NruI"Times New Roman%I test"Times New Roman)I dE test"Times New RomanI g,