d0d0LOCUS pBC SK + 3400 bp DNA SYN 26-Aug-2007 DEFINITION pBC SK + ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..3400 /organism="pBC SK +" /mol_type="other DNA" rep_origin complement(135..441) /label="f1_origin" misc_feature complement(459..614) /label="lacZ_a" misc_feature 585..607 /label="M13_pUC_fwd_primer" misc_feature 600..616 /label="M13_forward20_primer" promoter 626..644 /label="T7_promoter" misc_feature 669..685 /label="pBluescriptKS_primer" misc_feature complement(720..736) /label="pBluescriptSK_primer" promoter complement(772..791) /label="T3_promoter" misc_feature complement(808..826) /label="M13_reverse_primer" misc_feature complement(825..847) /label="M13_pUC_rev_primer" promoter complement(861..890) /label="lac_promoter" rep_origin complement(1199..1818) /label="pBR322_origin" CDS 2125..2784 /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene 2125..2784 /label="CAT/CamR" /gene="CAT/CamR" gene complement(3071..3271) /label="Ampicillin(861-660)" /gene="Ampicillin(861-660)" promoter complement(3314..3342) /label="AmpR_promoter" ORIGIN 1 CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC 61 ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA 121 GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 181 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC 241 CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 301 CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA 361 AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC 421 CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG 481 CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG 541 GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG 601 TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCGG 661 GCCCCCCCTC GAGGTCGACG GTATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG 721 ATCCACTAGT TCTAGAGCGG CCGCCACCGC GGTGGAGCTC CAGCTTTTGT TCCCTTTAGT 781 GAGGGTTAAT TGCGCGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 841 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 901 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 961 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 1021 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 1081 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 1141 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 1201 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 1261 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 1321 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 1381 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 1441 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 1501 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 1561 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 1621 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 1681 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 1741 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 1801 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 1861 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTCGACCGAA 1921 TAAATACCTG TGACGGAAGA TCACTTCGCA GAATAAATAA ATCCTGGTGT CCCTGTTGAT 1981 ACCGGGAAGC CCTGGGCCAA CTTTTGGCGA AAATGAGACG TTGATCGGCA CGTAAGAGGT 2041 TCCAACTTTC ACCATAATGA AATAAGATCA CTACCGGGCG TATTTTTTGA GTTGTCGAGA 2101 TTTTCAGGAG CTAAGGAAGC TAAAATGGAG AAAAAAATCA CTGGATATAC CACCGTTGAT 2161 ATATCCCAAT GGCATCGTAA AGAACATTTT GAGGCATTTC AGTCAGTTGC TCAATGTACC 2221 TATAACCAGA CCGTTCAGCT GGATATTACG GCCTTTTTAA AGACCGTAAA GAAAAATAAG 2281 CACAAGTTTT ATCCGGCCTT TATTCACATT CTTGCCCGCC TGATGAATGC TCATCCGGAA 2341 TTACGTATGG CAATGAAAGA CGGTGAGCTG GTGATATGGG ATAGTGTTCA CCCTTGTTAC 2401 ACCGTTTTCC ATGAGCAAAC TGAAACGTTT TCATCGCTCT GGAGTGAATA CCACGACGAT 2461 TTCCGGCAGT TTCTACACAT ATATTCGCAA GATGTGGCGT GTTACGGTGA AAACCTGGCC 2521 TATTTCCCTA AAGGGTTTAT TGAGAATATG TTTTTCGTCT CAGCCAATCC CTGGGTGAGT 2581 TTCACCAGTT TTGATTTAAA CGTGGCCAAT ATGGACAACT TCTTCGCCCC CGTTTTCACC 2641 ATGGGCAAAT ATTATACGCA AGGCGACAAG GTGCTGATGC CGCTGGCGAT TCAGGTTCAT 2701 CATGCCGTTT GTGATGGCTT CCATGTCGGC AGAATGCTTA ATGAATTACA ACAGTACTGC 2761 GATGAGTGGC AGGGCGGGGC GTAATTTTTT TAAGGCAGTT ATTGGTGCCC TTAAACGCCT 2821 GGTTGCTACG CCTGAATAAG TGATAATAAG CGGATGAATG GCAGAAATTC GAAAGCAAAT 2881 TCGACCCGGT CGTCGGTTCA GGGCAGGGTC GTTAAATAGC CGCTTATGTC TATTGCTGGT 2941 TTACCGGTTT ATTGACTACC GGAAGCAGTG TGACCGTGTG CTTCTCAAAT GCCTGAGGCC 3001 AGTTTGCTCA GGCTCTCCCC GTGGAGGTAA TAATTGACGA TATGATCCTT TTTTTCTGAT 3061 CAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC 3121 TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA 3181 CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA 3241 TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 3301 TTTATCAAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 3361 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC //H pBC SK + "Times New Romand0CH  f1_origin"Times New Romand0CH flacZ_a"Times New Romand0CɆH I_M13_pUC_fwd_pri"Times New Romand0H XhM13_forward20_pri"Times New Roman*d0CH rT7_pro"Times New Romand0CH pBluescriptKS_pri"Times New Romand0CɆH pBluescriptSK_pri"Times New Romand0H T3_pro"Times New Romand0CH (:M13_reverse_pri"Times New Romand0CH 9OM13_pUC_rev_pri"Times New Romand0CɆH ]zlac_pro"Times New Romand0H  pBR322_origin"Times New Romand0CH M ORF 1"Times New Romand0CH M CAT/CamR"Times New Romand0CɆH Ampicillin(861-660)"Times New Romand0H  AmpR_pro"Times New Roman d0FspI"Times New RomanH kzg d0ͰKpnI(657),ApaI(663),XhoI(668),SalI(674),ClaI(684),HindIII(689),EcoRV(697),EcoRI(701),PstI(711),SmaI(715),BamHI(719),SpeI(725),XbaI(731),NotI(738),EagI(738),SacII(750),SacI(759)"Times New RomanH  W d0MscI(2605),NcoI(2639)"Times New Roman- H 542h#A d0BstBI(2869),AgeI(2943)"Times New Roman5 H  d0BclI"Times New Roman H 0$rcr d0test"Times New RomanH {{f