Sequence of pBC_SK

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Sequence of pBC_SK_+

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Sequence of pBC SK + : 3400 BP; 877 A; 804 C; 863 G; 856 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60

1 gatttaacat tcgcaattat aaaacaattt taagcgcaat ttaaaaacaa tttagtcgag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120

61 taaaaaattg gttatccggc tttagccgtt ttagggaata tttagttttc ttatctggct 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180

121 ctatcccaac tcacaacaag gtcaaacctt gttctcaggt gataatttct tgcacctgag 180

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240

181 gttgcagttt cccgcttttt ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg 240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300

241 gattagttca aaaaacccca gctccacggc atttcgtgat ttagccttgg gatttccctc 300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360

301 gggggctaaa tctcgaactg cccctttcgg ccgcttgcac cgctctttcc ttcccttctt 360

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420

361 tcgctttcct cgcccgcgat cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg 420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... FspI(479)

421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 480

421 gtgtgggcgg cgcgaattac gcggcgatgt cccgcgcagg gtaagcggta agtccgacgc 480

.......... f1_origin .......... .......... lacZ_a ..........

.......... .......... .......... .......... .......... ..........

481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540

481 gttgacaacc cttcccgcta gccacgcccg gagaagcgat aatgcggtcg accgctttcc 540

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600

541 ccctacacga cgttccgcta attcaaccca ttgcggtccc aaaagggtca gtgctgcaac 600

.......... .......... lacZ_a .......... ....... M13_pUC_fwd_pri

.......... .......... .......... .......... .......... ..........

601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 660

601 attttgctgc cggtcactcg cgcgcattat gctgagtgat atcccgctta acccatggcc 660

lacZ_a M13_forward20_pri ...... T7_pro .......... ..........

ApaI,XhoI SalI ClaI,HindIII EcoRV(697),EcoRI(701)

661 gccccccctc gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg 720

661 cgggggggag ctccagctgc catagctatt cgaactatag cttaaggacg tcgggccccc 720

...... pBluescriptKS_pri ...... .......... .......... ..........

SpeI XbaI NotI738),EagI(738) SacII(750) SacI(759)

721 atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt 780

721 taggtgatca agatctcgcc ggcggtggcg ccacctcgag gtcgaaaaca agggaaatca 780

pBluescriptSK_pri .. .......... .......... .......... T3_pro

.......... .......... .......... .......... .......... ..........

781 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 840

781 ctcccaatta acgcgcgaac cgcattagta ccagtatcga caaaggacac actttaacaa 840

T3_pro .......... ....... M13_reverse_pri .... M13_pUC_rev_pri

.......... .......... .......... .......... .......... ..........

841 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 900

841 taggcgagtg ttaaggtgtg ttgtatgctc ggccttcgta tttcacattt cggaccccac 900

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

901 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 960

901 ggattactca ctcgattgag tgtaattaac gcaacgcgag tgacgggcga aaggtcagcc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 1020

961 ctttggacag cacggtcgac gtaattactt agccggttgc gcgcccctct ccgccaaacg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 1080

1021 cataacccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 1140

1081 ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 1200

1141 tgcgtccttt cttgtacact cgttttccgg tcgttttccg gtccttggca tttttccggc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 1260

1201 gcaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt ttagctgcga 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 1320

1261 gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa gggggacctt 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 1380

1321 cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac aggcggaaag 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 1440

1381 agggaagccc ttcgcaccgc gaaagagtat cgagtgcgac atccatagag tcaagccaca 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 1500

1441 tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 1560

1501 ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat agcggtgacc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 1620

1561 gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga tgtctcaaga 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 1680

1621 acttcaccac cggattgatg ccgatgtgat cttcctgtca taaaccatag acgcgagacg 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 1740

1681 acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt gtttggtggc 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 1800

1741 gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt tttcctagag 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 1860

1801 ttcttctagg aaactagaaa agatgcccca gactgcgagt caccttgctt ttgagtgcaa 1860

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttcgaccgaa 1920

1861 ttccctaaaa ccagtactct aatagttttt cctagaagtg gatctaggaa aagctggctt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat 1980

1921 atttatggac actgccttct agtgaagcgt cttatttatt taggaccaca gggacaacta 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt 2040

1981 tggcccttcg ggacccggtt gaaaaccgct tttactctgc aactagccgt gcattctcca 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttgtcgaga 2100

2041 aggttgaaag tggtattact ttattctagt gatggcccgc ataaaaaact caacagctct 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat 2160

2101 aaaagtcctc gattccttcg attttacctc tttttttagt gacctatatg gtggcaacta 2160

.......... .......... .......... ........ ORF 1 CAT/CamR ........

.......... .......... .......... .......... .......... ..........

2161 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc 2220

2161 tatagggtta ccgtagcatt tcttgtaaaa ctccgtaaag tcagtcaacg agttacatgg 2220

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag 2280

2221 atattggtct ggcaagtcga cctataatgc cggaaaaatt tctggcattt ctttttattc 2280

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa 2340

2281 gtgttcaaaa taggccggaa ataagtgtaa gaacgggcgg actacttacg agtaggcctt 2340

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ttacgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac 2400

2341 aatgcatacc gttactttct gccactcgac cactataccc tatcacaagt gggaacaatg 2400

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat 2460

2401 tggcaaaagg tactcgtttg actttgcaaa agtagcgaga cctcacttat ggtgctgcta 2460

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc 2520

2461 aaggccgtca aagatgtgta tataagcgtt ctacaccgca caatgccact tttggaccgg 2520

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt 2580

2521 ataaagggat ttcccaaata actcttatac aaaaagcaga gtcggttagg gacccactca 2580

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... MscI(2605) .......... .......... NcoI(2639)

2581 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc 2640

2581 aagtggtcaa aactaaattt gcaccggtta tacctgttga agaagcgggg gcaaaagtgg 2640

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat 2700

2641 tacccgttta taatatgcgt tccgctgttc cacgactacg gcgaccgcta agtccaagta 2700

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc 2760

2701 gtacggcaaa cactaccgaa ggtacagccg tcttacgaat tacttaatgt tgtcatgacg 2760

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct 2820

2761 ctactcaccg tcccgccccg cattaaaaaa attccgtcaa taaccacggg aatttgcgga 2820

........ ORF 1 CAT/CamR ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc gaaagcaaat 2880

2821 ccaacgatgc ggacttattc actattattc gcctacttac cgtctttaag ctttcgttta 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcgacccggt cgtcggttca gggcagggtc gttaaatagc cgcttatgtc tattgctggt 2940

2881 agctgggcca gcagccaagt cccgtcccag caatttatcg gcgaatacag ataacgacca 2940

.......... .......... .......... .......... .......... ..........

AgeI(2943) .......... .......... .......... .......... ..........

2941 ttaccggttt attgactacc ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc 3000

2941 aatggccaaa taactgatgg ccttcgtcac actggcacac gaagagttta cggactccgg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... BclI(3057)

3001 agtttgctca ggctctcccc gtggaggtaa taattgacga tatgatcctt tttttctgat 3060

3001 tcaaacgagt ccgagagggg cacctccatt attaactgct atactaggaa aaaaagacta 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 caaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 3120

3061 gttttcacga gtagtaacct tttgcaagaa gccccgcttt tgagagttcc tagaatggcg 3120

.......... .......... ..... Ampicillin(861-660) ..... ..........

.......... .......... .......... .......... .......... ..........

3121 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 3180

3121 acaactctag gtcaagctac attgggtgag cacgtgggtt gactagaagt cgtagaaaat 3180

.......... ..... Ampicillin(861-660) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 3240

3181 gaaagtggtc gcaaagaccc actcgttttt gtccttccgt tttacggcgt tttttccctt 3240

.......... ..... Ampicillin(861-660) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 3300

3241 attcccgctg tgcctttaca acttatgagt atgagaagga aaaagttata ataacttcgt 3300

..... Ampicillin(861-660) ..... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tttatcaagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 3360

3301 aaatagttcc caataacaga gtactcgcct atgtataaac ttacataaat ctttttattt 3360

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 caaatagggg ttccgcgcac atttccccga aaagtgccac .......... .......... 3400

3361 gtttatcccc aaggcgcgtg taaaggggct tttcacggtg .......... .......... 3400

.......... .......... .......... .......... .......... ..........