c/c/(#LOCUS pBG1 5715 bp DNA SYN 20-Sep-2007 DEFINITION pBG1 ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5715 /organism="pBG1" /mol_type="other DNA" misc_feature complement(29..51) /label="pGEX_3_primer" misc_feature complement(2013..2880) /label="ars1" misc_feature complement(3279..3422) /label="lacZ_a" misc_feature 3393..3415 /label="M13_pUC_fwd_primer" misc_feature 3408..3424 /label="M13_forward20_primer" misc_feature complement(3490..3508) /label="M13_reverse_primer" misc_feature complement(3507..3529) /label="M13_pUC_rev_primer" promoter complement(3543..3572) /label="lac_promoter" rep_origin complement(3881..4500) /label="pBR322_origin" CDS complement(4655..5515) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4655..5515) /label="Ampicillin" /gene="Ampicillin" promoter complement(5557..5585) /label="AmpR_promoter" ORIGIN 1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA 61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG 121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC 181 ACCATATGCA GGTCGACTCT AGAGGATCCC CATCACCTGC ATCTTAGGTT AATTATTAAT 241 TAATCTCATC GATCGCTTTC TTCTTACTTT AACAAAAACA CATCGAAAGA GATACGATGT 301 TTGATTTGAA TACTTGTCTT CGTAAAAATA TCTTAGAACT TCAGCCTTAT CGCTGTGCGA 361 GAGAGTATGT AATAACTTGA TAAACTAGAA AGCGTGATAT ATATTAACAT AGAATATAGT 421 GACTTCTCTG AGTATGTTTT ATCTTTATAT TTGGGTAATA ATTGATATGA GGGCTACTTC 481 TAGAAAGATA TTTTATTAGC TGCTTGGAAA GCAATGAAGT ACTCCCTGTT TACTGTTGTT 541 GTTGGAGCTG GCGATTTCAG GCCTTTGTTG TTTTTTACCC TTTTCTCGCC TATATATTTT 601 TTGCTAATCT TAATAGAGGT GTCCTTCTTG ATGCCAATGA ATGTGCTTAT GGTTCGGTTA 661 TTTCCGTGGA CGGTGTGGAA TTCAATCGTT ATCCGGATCC TAGACAGATC GAAGTAAAGC 721 AAAGATTATG TGATTTGAGA AACAAAGAGC TTTCCATTAC CAAGCCACTA ACACCAGATA 781 ACATTTGCAT GGGTGTTGGC AGCGATGAAA TTATTGACTC CTTAATTCGC ATTTCTTGTA 841 TTCCTGGAAA AGATAAGATT TTGATGTGTC CACCTTCGTA CGGAATGTAT ACGGTCTCTG 901 CAAAAATCAA CGATGTTGAG GTTGTTAAGG TTCTCCTTGA ACCAGATTTC AATTTGAACG 961 TCGATGCTAT TTGCGAAACA CTTTCTAAAG ATAGTGCCAT CAAAGTCTTT TTTGCTTGTT 1021 CCCCTGGTAA TCCAACAGCT AAGGCTCTTA AACTCGAGGA TATTAAGAAG ATTTTGGAGC 1081 ACCCTACATG GAATGGAATC GTTGTCGTCG ATGAGGCCTA TATTGATTTC TCTGCTCCCG 1141 ATATGTCTGC CTTAACTCTT GTCAATGAAT ATCCAAACCT TGCTGTTTGC CAAACCCTTT 1201 CGAAATCTTT CGGTCTAGCA GGAATTCGGT AAGTTAATCT AAGCTTTTAA GCATTAATAT 1261 TATTTCTTTA CTAACTATGA TGTTTAGTAT CGGATTTTGT TTGACAAGTA AACCTATTGC 1321 TACCATTATG AACTCATTAA AGGCTCCTTA TAACATTAGC GAACCCACCT CTCGTTTAGC 1381 TTTGGATGCT TTATCCCCTC AGTCGATTGA TAAAATGCAT ACATATAGAG ATGCTATTAT 1441 ACAGCAAAGA GTTCGTCTTT GCAAAGAGCT TACTACCATT AAAGGAATGG GCAAGATTAT 1501 AGGTGGTTAT GATGCAAATT TTATCCTCAT TCAGGTTTTA GATAGACCAG AAGGAGGTAA 1561 GCCTAGTAAC GATGCCGCAA AATATCTTTA TTTACAAATG GCCACGATGC ACAAAGTCGT 1621 TGTTAGATTT AGAGGTACAG AGCCTCTTTG CGAAGGAGCA TTGCGTATCA CTGTTGGCAC 1681 AGAAGAAGAG AATACGATAT TACTAAAAAC TATTAAACTT GTATTGCAGG AATACTATAC 1741 AAAAAAATGA GGTTTCCTTT TCTGGCCTAT TAGAACGTTT CAATTAGACA AATTATAACT 1801 CTTTCCTTTC CCCTTCCGGA CTCGTCACAT TTCTTTTCTG ATGTACCCAA TATATTTTGT 1861 GTTCGCTAAT TGCGCTTGCA TTCCTTCTTT CTTTATTAAA ATACGTTTTC TTCCTTTTTG 1921 GTGTGTTTCC GTATAGTTCG ATAAGCAATG AAAAGATGTT ATTTTTAATT TTGTAAAGGA 1981 GTTACGCTAA TTTGGGTACA ATTCGAGTCT AACTCCTTAA CCACTCGGAC ATAGTGACTT 2041 ATCTGACACT CATTGTAAAT TAATATATAT ATAGGCATTT TGTTTAGTTA AAGGTACTTA 2101 AGTAATTAGT ATAAACGAAC CAATTTTATA ATCAGGAAGT TAAGTGAATG GTAGCACATG 2161 TCGTAAAAAT TGTGAATTTT TATTGAATAA TATTTTAAAT ACAAGCCTTT CTAGACTAGG 2221 TATACTCATA AACATATATG AGCAAAAGGA TAGAGGAGAT TACATTGCAT CTTCTACAAA 2281 TTTATTTATT GCCCTTTACT GAAAAATTAA ATAATGAGTA CTAAATGATA AAAAGCGCTC 2341 AGTACAAGAA AGATTGCAAA AATATTGCAT TCTTCATGAA TTAAAGTTGC ATATAAGGCA 2401 TATTGAAAGT AATAGTACTA AAACAGCAGT TAGCGAAAAT TAATAGAATT ATATTCGCAA 2461 GACAATTGTG ACATTAAATT AAAAAATTGT AGAATTTTTA CTATCCTCTT TAACGCCATG 2521 AGCCTTTATA AAAAGGTTAA ATTAGTTTTA ACATTCTTTT TTTGAGTAAG AGTTTACAAT 2581 TTATCAAAAC CTGTGTTATT ATATTCATTA GTTTCAATTT ATTAGCATCT AGAGAAAAAT 2641 CAATTGGCAG TTACATTGTT GGAATTTATG AAGAAAAAGA ATCTACAACG GAGAATAAGT 2701 TGCTGATCGC TTTCCCTAAA ATTGTATATT TTGCTGAGCT TATTTTGACA TTTCGTTGAA 2761 GTTTTCTCTA ATTCGCATTC ATTTTAAGTA AAACAATGAG AAATAAAATT ACAAAAAATA 2821 CAAATTAAAA TACAATTTTT AGCTATAATT ATAGACGATG CCCTTGTATC CCATTCTGTC 2881 TCGCTTGCCC CTACTTTTTA TCTTTTATAT ACCATAATGA ACGCTGCCGC TACTAACCAT 2941 ACCCCGATTT TACATTTCGG ACTCCCAAGG ACGTACAAAA TAGAAAACTA TAGAAAAAAA 3001 TAATCAGAAA ATAGCATGTC ATCTCTTTGT AAAACGCGTT TGCAAGAAGA AAGGAAACAA 3061 TGGAGAAGAG ATCATCCATT TGTATGTAAA TTTTAGTAAA CTTGAAGAAA TCACTAACAA 3121 CTTCTCTTAC TTAGGGATTC TATGCAAAAC CTTGTAAATC ATCTGATGGA GGACTCGATT 3181 TAATGAATTG GAAGGTTGGA ATTGTACCGA GCTTATGCGG TGTGAAATAC CGCACAGATG 3241 CGTAAGGAGA AAATACCGCA TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA 3301 AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC 3361 AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC 3421 CAGTGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCCCCGGGTA CCGAGCTCGA 3481 ATTCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC 3541 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC 3601 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC 3661 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG 3721 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC 3781 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT 3841 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC 3901 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA 3961 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 4021 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG 4081 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC 4141 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC 4201 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA 4261 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 4321 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG 4381 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT 4441 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT 4501 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA 4561 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 4621 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC 4681 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA 4741 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC 4801 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA 4861 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA 4921 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG 4981 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC 5041 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG 5101 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT 5161 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT 5221 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA 5281 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC 5341 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC 5401 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA 5461 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT 5521 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT 5581 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC 5641 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA 5701 CGAGGCCCTT TCGTC //DpBG1 "Times New Romanc/CD3 pGEX_3_pri"Times New Romanc/CD@ ars1"Times New Romanc/CɆD ^ lacZ_a"Times New Romanc/DA W M13_pUC_fwd_pri"Times New Romanc/CDP ` M13_forward20_pri"Times New Roman*c/CD M13_reverse_pri"Times New Romanc/CɆD M13_pUC_rev_pri"Times New Romanc/D lac_pro"Times New Romanc/CD) pBR322_origin"Times New Romanc/CD/ORF 2"Times New Romanc/CɆD/ Ampicillin"Times New Romanc/DAmpR_pro"Times New Roman c/NdeI(184),ClaI(249)"Times New RomanDgS$E$'aE c/XhoI(1053),BstBI(1200)"Times New RomanD( c/MscI"Times New RomanADtx{t5 c/AflII"Times New Roman1D  c/NarI"Times New Roman D})t:V:I c/+PstI(3444),SmaI(3465),KpnI(3471),SacI(3477)"Times New Romant D`TGG[ c/AatII"Times New RomanDcO;,; c/test"Times New Roman$DcsaU c/test"Times New RomanL Djrzz'